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In order to enhance natural products value, Melissa officinalis (lemon balm) aerial part (leaves) has been studied in this work. Hence, the objective of this study is to determine the chemical composition of the studied plant polyphenols extracts using HPLC/DAD, as well as evaluate their flavonoid extracts' antioxidant and antimicrobial activities using DPPH⢠and disk diffusion methods, respectively. The results of phenols chemical composition showed the existence of two phenolic acids, five flavonic aglycones and six heterosides, while the biologic results of the plant flavonoid extracts exhibited the existence of a good antioxidant and antimicrobial activities.
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Teucrium polium L. is commonly used in folk medicine to treat hypertension and diabetes and to heal wounds. The present work aimed to evaluate the different biological activities of T. polium hydroalcoholic extract, its total phenol and flavonoid content, and its mineral elements. Results showed that T. polium extract showed significant antioxidant potential in 2-diphenyl-1-picrylhydrazyl (DPPH) assay with IC50 equal to 8.68 µg/mL but with moderate activity in galvinoxyl assay with IC50 of 21.82 µg/mL and mild activity in the ß-carotene assay. It also showed a pronounced anti-hyperglycemic activity using α-amylase inhibitory assay (IC50 = 111.68 µg/mL) and exceeds that of acarbose. T. polium showed excellent activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with IC50 values of 28.69 and 4.93 µg/mL, respectively, postulating its promising anti-Alzheimer potential. The plant extract exhibited a strong anti-inflammatory effect with Bovine Serum Albumin (BSA) denaturation inhibitory potential estimated by 97.53% at 2 mg/mL, which was further confirmed by the in vivo carrageen-induced edema model. The extract revealed its richness in flavonoids and phenols, evidenced by its polyphenols content (36.35 ± 0.294 µg GAE/mg) and flavonoids (24.30 ± 0.44 µg QE/mg). It is rich in minerals necessary for human health, such as calcium, potassium, iron, sodium, magnesium, manganese and zinc. Molecular docking performed for previously identified compounds on human α-amylase, 5-lipoxygenase (5-LOX) and acetylcholine esterase confirmed the results. Thus, it can be concluded that T. polium can be a good candidate for alleviating many health-debilitating problems and can be highly beneficial in the pharmaceutical industry and medical research.
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Lobularia libyca (L. libyca) is a traditional plant that is popular for its richness in phenolic compounds and flavonoids. The aim of this study was to comprehensively investigate the phytochemical profile by liquid chromatography, electrospray ionization and tandem mass spectrometry (LC-ESI-MS), the mineral contents and the biological properties of L. libyca methanol extract. L. libyca contains significant amounts of phenolic compounds and flavonoids. Thirteen compounds classified as flavonoids were identified. L. libyca is rich in nutrients such as Na, Fe and Ca. Moreover, the methanol extract of L. libyca showed significant antioxidant activity without cytotoxic activity on HCT116 cells (human colon cancer cell line) and HepG2 cells (human hepatoma), showing an inhibition zone of 13 mm in diameter. In silico studies showed that decanoic acid ethyl ester exhibited the best fit in ß-lactamase and DNA gyrase active sites; meanwhile, oleic acid showed the best fit in reductase binding sites. Thus, it can be concluded that L. libyca can serve as a beneficial nutraceutical agent, owing to its significant antioxidant and antibacterial potential and due to its richness in iron, calcium and potassium, which are essential for maintaining a healthy lifestyle.
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Antioxidantes , Brassicaceae , Antibacterianos/farmacologia , Antioxidantes/análise , Antioxidantes/farmacologia , Flavonoides/análise , Humanos , Metanol/química , Fenóis/análise , Compostos Fitoquímicos/química , Extratos Vegetais/química , Extratos Vegetais/farmacologiaRESUMO
Spices in general owned a very important reputation in the herbal plantae Kingdom; they have been used for food flavoring, preservation, aroma, and coloring for more than 2000 years. Moreover, spices are classified as all natural, and for this reason, they attracted a large scale of consumers worldwide; however, since most of spices are consumed in their natural form (without grounded them), it is necessary to monitor their consumed quantities. Hence, the aim of this work was to quantify major and trace elements contained in some Algerian kitchen frequently used spices (Piper nigrum L., Nigella sativa L., and Pimpinella anisum L.). Spice materials were washed well then pass through an instrumental neutron activation analysis (INAA). Results revealed the existence of twenty-six elements among them: K, Ca, Fe, and Na which showed significant concentrations, consecutively, while Ce, Eu, Lu, Sm, and Tb were presented at low concentrations. Furthermore, these spices consumption assessment results were found to be well below the tolerance limits compared with the recommended values (RDA) suggested by the FAO association.
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Especiarias , Oligoelementos , Argélia , Humanos , Análise de Ativação de Nêutrons , Medição de Risco , Especiarias/análise , Oligoelementos/análiseRESUMO
This study describes the minerals elements, chemical composition, antioxidant and antimicrobial activities of Algerian Melissa officinalis plant. The essential oil (EO) was extracted by hydrodistillation (HD) using a Clevenger-type apparatus of dry leaves of M. officinalis and was analyzed by two techniques, gas chromatography coupled with flame ionization (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Eighteen minerals comprising both macro- and microelements (As, Br, K, La, Na, Sb, Sm, Ba, Ca, Ce, Co, Cr, Cs, Fe, Rb, Sc, Th, and Zn) were determined using neutron activation analysis technique for the first time from Algerian Melissa officinalis plant. Seventy-eight compounds were identified in the essential oil, representing 94.090% of the total oil and the yields were 0.470%. The major component was geranial (45.060%). Other predominant components were neral (31.720%) and citronellal (6.420%). The essential oil presented high antimicrobial activity against microorganisms, mainly five human pathogenic bacteria, one yeast, Candida albicans, and two phytopathogenic fungi. The results can be used as a source of information for the pharmaceutical industry and medical research.
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A versatile gamma ionization chamber (VGIC) has been designed, developed and characterized, in order to study experimentally its characteristics according to the geometry of the electrodes, the volume and pressure of the filler gas for the design of a gamma sealed chamber. The tests were conducted under the IEC (International Electro-technical Commission). The results obtained in various nuclear tests of the characterization and calibration of the ionization chamber gamma VGIC developed at our laboratory were presented in this paper. Three irradiators were operated, irradiator intensive gamma (60Co: 1.25 MeV), medium intensive gamma (137Cs: 0.662 MeV) and low-intensity gamma (60Co). Saturation curves and linearity were identified and the operating range and the sensitivity of the chamber have been deducted. The current, voltage (I,V) characteristics of the chamber filled, with argon gas at 0.4 M pa pressure, for gamma ray irradiator sources were studied, the chamber was irradiated with gamma rays using different gamma sources. The plateau region is reached above 200 V and the detector operating voltage is found to be 600V. It is observed that in the plateau region the slope is constant with an increase in the exposure rate. The (1/I, 1/V) and (I, l/V2) characteristic curves reveal the presence of the initial and volume recombination losses. The volume recombination losses are found to be smaller than the initial recombination losses.
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This study describes the chemical composition, antitumor, antioxidant, and antimicrobial activities of the plant Thymus algeriensis Boiss. Essential oils (EOs) were collected in different periods (before, during, and after flowering stage) from the El-Guetfa region, M'sila, Algeria. The EOs extraction was achieved using three distinguishing techniques: hydro (Clevenger trap), steam, and microwave distillations, targeting different aerial parts of the plant (stems, flowers, and leaves). The EOs chemical components were estimated using GC-FID and GC-MS apparatuses. The resulting yield of the extracted oil was moderate and ranged between 0.84 and 1.53% (wt/vol). In total, eighty-five components were identified, in which the oxygenated monoterpenes family formed the main portion, starting from 40.56 up to 70.66%. The obtained essential oil was dominated by five major components that varied from low to quite moderate percentages: camphor (17.45-32.56%), borneol (11.16-22.2%), camphene (7.53-12.86%), 1.8-cineole (5.16-11.21%), and bornyl acetate (3.86-7.92%). The biological results of this oil pointed out that the EOs extracted from the leaves part exposed a weak radical scavenging activity afterward using two well-known antioxidant assays DPPH (IC50 = 8.37 mg/mL) and ABTS (10.84 mg/mL). Meanwhile, this oil presented strong inhibition activity against colon cancer cell line HCT116 (LC50 = 39.8 µg/mL) and a moderate inhibitory against hepatocellular cancer cells HePG2 (LC50 > 100 µg/mL). In addition, this oil antimicrobial activity was quite important against Micrococcus luteus (M. luteus), Staphylococcus aureus CIP 7625, Escherichia coli ATCC 10536, Saccharomyces cerevisiae ATCC 4226, Candida albicans IPA200, Candida tropicalis (Ct), and Candida glabrata (Cg) after using Amoxicillin and Itraconazole as references.
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Our study evaluated the in vitro antioxidant properties, antibacterial and antifungal activities, anti-inflammatory properties, and chemical composition of the essential oils (EOs), total phenol, and total flavonoid of wild Mentha pulegium L. This study also determined the mineral (nutritional and toxic) elements in the plant. The EOs were extracted using three techniques-hydro distillation (HD), steam distillation (SD), and microwave-assisted distillation (MAD)-and were analyzed using chromatography coupled with flame ionization (GC-FID) and gas chromatography attached with mass spectrometry detector (GC-MS). The antioxidant effects of the EOs were tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), while the antibacterial and antifungal activities of the EO and methanolic extract were tested using Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Twenty-six compounds were identified in the essential oil, representing 97.73% of the total oil, with 0.202% yield. The major components were pulegone (74.81%), menthone (13.01%) and piperitone (3.82%). Twenty-one elements, including macro- and micro-elements (Ba, Br, Ca, Cl, Co, Cr, Cs, Eu, Fe, K, Mg, Mn, Mo, Na, Rb, Sb, Sc, Sr, Th, U and Zn), were detected using neutron activation analysis (INAA) and inductively coupled plasma optical emission spectrometry (ICP-OES), with the concentration of mineral element close to the FAO recommendation. The results show that the EOs and extracts from Mentha pulegium L. had significant antimicrobial activities against the microorganisms, including five human pathogenic bacteria, one yeast (Candida albicans), and one phytopathogenic fungi. The in vivo anti-inflammatory activities of the leaf extracts were confirmed. The results indicate that the EOs and extracts from Mentha pulegium L. have promising applications in the pharmaceutical industries, clinical applications, and in medical research.
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Flavonoides/química , Mentha pulegium/química , Minerais/química , Óleos Voláteis/química , Extratos Vegetais/química , Polifenóis/química , Animais , Anti-Infecciosos/farmacologia , Antioxidantes/farmacologia , Monoterpenos Cicloexânicos/química , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Masculino , Mentol/química , Camundongos , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologiaRESUMO
In this study, two kinds of Artemisia plant, Artemisia campestris L. and Artemisia herba-alba Asso., collected from different locations in Djelfa province, Algeria, were subjected to an instrumental neutron activation analysis (INAA) in order to determine their essential and toxic elements for the first time. The obtained results for both types revealed the existence of twenty-one elements, namely, As, Ba, Br, Ca, Ce, Co, Cr, Cs, Eu, Fe, Hf, K, La, Na, Rb, Sb, Sc, Sm, Sr, Yb, and Zn, where, the elements K, Ca, Fe, and Na respectively showed a significant concentration. On the other hand, the tolerable daily intake (TDI) of the studied plants for an adult person per day was within the tolerance limits imposed by the World Health Organization (WHO). Hence, these findings might therefore be used to offer scientific basis for an optimum usage of the studied plants and so enriches the database of medicinal herbs.
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Artemisia , Oligoelementos , Adulto , Argélia , Humanos , Análise de Ativação de Nêutrons , Sódio , Oligoelementos/análiseRESUMO
In Algeria, Data and studies on the non-metal trace element selenium (Se) are presently lacking, therefore, the aim of this investigation is to provide new data on (Se) element via its determination for the first time from Mentha pulegium L. plant. The plant samples were collected in summer of 2012 from Ain-Oussera region, Djelfa province, Algeria; they were dried and powdered. After the neutron irradiation, the samples were digested using high oxidative reagents including H2SO4, HNO3, H2O2 and HCl. The end of this process gave two phases, organic and aqueous discard phase. By using a separating funnel, the organic phase was transferred into a vial in order to measure their induce radionuclide 75Se using gamma-ray spectrometer. A non-chromatographic and sensitive analytical technique RNAA (Radiochemical Neutron Activation Analysis), was applied in this investigation due to its great significant minor systematic error. Results were determined using two distinguish calculation methods, relative-RNAA and k0-RNAA, the findings were quite significant, whereas, the average separation yield was about 85% for both calculation methodologies. Moreover, (Se) concentration obtained from M. pulegium L., is close to the minimal FAO (Food and Agriculture Organization of the United Nations) recommended consumption.
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Extração Líquido-Líquido/métodos , Mentha pulegium/química , Radioquímica/métodos , Selênio/análise , Humanos , Selênio/toxicidade , Espectrometria gamaRESUMO
Instrumental neutron activation analysis (INAA) has been used to determine the concentration of some toxic chemical elements in a variety of aromatic plants samples collected from Djelfa region. In the present work, eight medicinal plants were examined, such as Artemisia herba-alba Asso., Artemisia compestris L., Laurus nobilis L., Origanum vulgare L., Mentha spicata L., Rosmarinus officinalis L., Mentha pulegium L., and Pistacia lentiscus L. The levels of toxic elements were compared to their daily total intake; Arsenic was present in all plant species examined, with a concentration ranging from 0.18 to 5.44 µg g- 1. Bromine was also detected in all the medicinal plant species, with high concentrations, compared to arsenic except in the case of Laurus nobilis that has the highest concentration of arsenic. Cerium, cobalt, chromium, and antimony were presented in all plant species. The exactitude of the results was assessed by analyzing the certified reference material of SRM-NIST 1573a and CRM GB07605 (GSV4). These data analysis for this medicinal plant can be useful for therapeutics and pharmaceutical purposes.
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Antimônio/análise , Arsênio/análise , Bromo/análise , Cério/análise , Cromo/análise , Cobalto/análise , Plantas Medicinais/química , Plantas Medicinais/toxicidade , Argélia , Antimônio/toxicidade , Arsênio/toxicidade , Bromo/toxicidade , Cério/toxicidade , Cromo/toxicidade , Cobalto/toxicidadeRESUMO
This study presents the elemental concentration obtained in two Algerian spices (Coriandrum sativum L. and Cuminum cyminum L.) evaluated by instrumental neutron activation analysis (INAA), in order to highlight the importance of these spices as a potential source of micronutrients. The daily intake of micronutrients and potentially toxic elements were determined and compared with the recommended values (RDA) and were found to be well below the tolerance limits. Twenty-two elements were assessed, eight essential chemicals were quantified with tendency K > Ca > Na > Fe > Zn > Cr > Co > Se, and three potential toxic elements were present in the descending content pattern Br > As > Sb.