RESUMO
Microalgae have been reputed as novel biological materials due to their unique structure, surface functionality and optical activity, making them worthwhile agents in biosensing and theranostic applications. However, further scrutiny is required for utilizing them in routine optical techniques due to their complex structure and diverse chemical components. Here, laser induced fluorescence (LIF) features of a bio-compatible microalgae i. e. Arthrospira platensis (Spirulina) have been assessed. Typical fluorescence properties as well as the inner filter effects (IFEs) were examined and revealed to be strongly dependent on concentration, excitation wavelength, and detection geometry as well. IFEs and resulting spectral shifts have been analyzed considering various SP chromophores, reabsorption processes, and resonance energy transfer (RET) mainly from "Carotenoids to Phycobilisomes" as well as "Phycobilisomes to Chlorophyll-a". As a result, LIF spectral shift due to the re-absorption events (secondary-IFE) is introduced as a credible parameter for design of precise fluorescence-based sensors, due to being less dependent on ambient noises. We hope that the findings provide novel features regarding the LIF of Spirulina (SP) that could be utilized to design and develop optical sensors in the field of photonics, material diagnosis and biomedical theranostics.
