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1.
Cureus ; 16(8): e67247, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39301345

RESUMO

Bibliometry is a popular research method that is used to explore and analyze large volumes of data in an effort to highlight trends, patterns, and impacts within a specific field. This review aimed at highlighting the characteristics and citation patterns of the high-impact bibliometric research studies that were published in the neurosurgical literature. Using PubMed and Google Scholar, the 50 (52 due to identical citation numbers for the lowest three articles) most cited bibliometric research publications were identified and reviewed. Information relating to the articles' publication and bibliometric features were retrieved. The articles' citation numbers were collected. The median article age and journal impact factor (IF) were eight years and 2.76, respectively. The majority of studies were published in World Neurosurgery and the Journal of Neurosurgery, which were the publishing journals for 18 (35%) and 12 (23%) articles, respectively. Twenty-six (50%) articles were first authored by researchers from the United States of America (USA). The highest bibliometric component was science mapping, which was the theme in 30 (58%) articles. The majority of the bibliometric focus was clinical topics/fields (22 (42%) articles) and neurosurgeons/departments (21 (40%) articles). The most popular bibliometric metric was the h-index (±variants), which was employed in 22 (42%) articles. The median size of analyzed data was 188, and the most frequently utilized databases were Scopus (22 (42%) articles) and Web of Science (21 (40%) articles). The median (range) citation numbers were 52 (29-238). The citation analysis showed significantly higher citation numbers for older articles (aged ≥ 8 years) and studies published in the Journal of Neurosurgery. The citation rates were not influenced by the size of the data, the searched databases, or the bibliometric features. In conclusion, the most cited bibliometric research publications in the neurosurgical literature were predominantly descriptive analyses of clinical topics/fields and performance analyses of neurosurgeons/departments. Their citation numbers were relatively modest and were positively influenced by the publication's age and by a specific publishing journal but not by the bibliometric features of the study. Bibliometric research provides useful analytic tools that can be utilized in review studies and other practical purposes such as scholarly practices and policy decision-making.

2.
Ann Med Surg (Lond) ; 86(9): 5276-5282, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39239065

RESUMO

Background: Surgical correction of post-infarct ventricular septal defect (PIVSD) is associated with a significant incidence of morbidity and mortality. The authors aimed to evaluate the effectiveness and safety of surgical versus transcatheter approaches in the management of PIVSD. Methods: A systematic review and meta-analysis of retrospective from five databases including the Cochrane Library, PubMed, Web of Science, Ovid, and Scopus) until 9 March 2024 was conducted. Risk ratio (RR) for dichotomous outcomes was used and data with a 95% CI are presented. Results: A total of 7 retrospective observational studies with 603 patients were included in the analysis. Surgical closure was associated with a significantly lower short-term mortality and lower number of residual shunt or re-intervention rate compared to percutaneous closure, with a relative risk (RR) of 1.21 (95% CI:1:00-1.46, P = 0.05) and 2.68 (95% CI: 1.46-4.91, P = 0.001), respectively. Surgical closure was associated with a non-significantly lower long-term mortality rate compared to percutaneous closure, with a relative risk (RR) of 1.10 (95% CI: 0.82-1.48, P = 0.52). No difference is reported when time from acute myocardial infarction (AMI) or PIVSD to intervention is compared groups, with a relative risk (RR) of -0.24 (95% CI: -4.49 to 4.2, P = 0.91). Conclusion: Our meta-analysis shied the light on the significance of surgical closure in terms of short-term mortality and the need for re-intervention. However, no significant difference was observed in terms of long-term mortality and time to intervention.

3.
Cureus ; 16(7): e64785, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39156328

RESUMO

Survey research enables the gathering of information on individual perspectives in a large cohort. It can be epidemiological, attitude or knowledge focussed. Assessment of survey studies sampling neurosurgeons is currently lacking in the literature. This study aimed to highlight the characteristics, quality, and citation predictors of the most influential survey research studies published in the neurosurgical literature. Using PubMed and Google Scholar, the 50 most cited survey research publications were identified and reviewed. Data relating to the characteristics of the articles, participants and questionnaires were retrieved. The studies' quality and citation patterns were assessed. The median articles' age and publishing journal impact factor (IF) were 15.5 years and 2.82, respectively. Thirty-two (64%) articles were first authored by researchers from the USA while 28(56%) studies were focussed on specific disease management. The median number of participants and response rates were 222 and 51%, respectively. A full version of the questionnaire was provided in 18 (36%) articles. Only four (8%) articles reported validation of the questionnaire. The overall quality of reporting of the surveys was considered fair (based on good grading in five parameters, fair grading in one parameter, and poor grading in four parameters). The median citation number was 111. The citation analysis showed that the participant number, article age (≥15.5 years), and questionnaire category (surgical complications) were significant predictors of citation numbers. The citation rates were not influenced by the response rates or the journal's IF. In conclusion, high-impact survey publications in the neurosurgical literature were moderately cited and of fair quality. Their citation numbers were not affected by response rates but were positively influenced by the publication age, number of participants, and by novel data or the questions raised in the survey category. Surveys are valuable forms of research that require extensive planning, time, and effort in order to produce meaningful results. Increasing awareness of the factors that could affect citations may be useful to those who wish to undertake survey research.

4.
Sci Rep ; 14(1): 17506, 2024 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-39080349

RESUMO

In this paper, a single stage buck-boost DC-AC converter based on coupled inductors is presented for renewable energy and electric vehicle applications. The proposed topology works with only three semiconductor switches, two diodes, and three coupled inductors to transfer input DC voltage to a high gain or low gain output AC voltage. A coupled inductor is used instead of normal inductors, which will reduce core and size requirements. The sinusoidal pulse width modulation strategy is used in this paper for controlling the main switch. There are many merits in the presented topology, like high gain up to five times of input voltage, compact size, less number of components, which results in reducing the overall cost, reducing switching loss, and increasing the converter efficiency. The simulation study is carried out using MATLAB/SIMULINK to simulate the operation of the proposed converter. Also, an experimental setup is built up to examine the actual operation of the proposed converter. There is a good agreement between simulation and experimental results which increases the validation and confidence of the model.

5.
Ann Med Surg (Lond) ; 86(5): 2591-2597, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38694304

RESUMO

Background: In Palestine, cervical cancer is the third most common gynaecological cancer, exhibiting higher mortality rates compared to regional counterparts. Late-stage diagnosis and limited awareness contribute to this disparity. This study aims to assess the awareness of Palestinian medical students regarding cervical cancer prevention. Methods: A questionnaire-based survey involving 532 medical students from various Palestinian universities was conducted between October 2023 and December 2023. The survey encompassed closed-ended quantitative and demographic questions distributed through social media. Results: The study was carried out on a group of 532 medical students (66.3% women and 33.6% men), (average age 21.7 years). Most students (77.7%) knew that the main risk factor for cervical cancer was human papillomavirus (HPV) infection. Participants doing the internship had the greatest knowledge, whereas students from the first year had the poorest knowledge about the main risk factors for cancer of the cervix. Only about half, 50.2% of all students, knew that in Palestine, there is no screening program. 84.4% of students correctly indicated that the cervical cancer screening test is a pap smear test. 41.4% of respondents knew that microscopic evaluation of the exfoliated cells from the vaginal part of the cervix is the screening test for cervical cancer which is used. Only about half of the students (45.1%) knew that there were no specific early symptoms of cervical cancer. Conclusions: This study highlights the need for enhanced education, particularly regarding screening program awareness, among Palestinian medical students. Addressing these knowledge gaps is crucial for effective preventive strategies.

7.
Foods ; 12(22)2023 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-38002190

RESUMO

This study investigated the effects of fermentation and germination on the physicochemical, nutritional, functional, and bioactive quality attributes of samh seeds. Regardless of the processing treatment, samh seeds were found to be a rich source of phenolic compounds, namely gallic acid (79.6-96.36 mg/100 g DW), catechol (56.34-77.34 mg/100 g DW), and catechin (49.15-84.93 mg/100 g DW), and they possessed high DPPH antiradical activity (65.27-78.39%). They also contained high protein content (19.29-20.41%), essential amino acids content (39.07-44.16% of total amino acids), and unsaturated fatty acid content (81.95-83.46% of total fatty acids) and a low glycemic index (39.61-41.43). Fermentation and germination increased L*, b*, foaming capacity, oil absorption capacity (OAC), water absorption capacity (WAC), swelling power, microbial counts, antioxidant activity, total flavonoid content (TFC), total phenolic content (TPC), in vitro protein digestibility, protein efficiency ratio, and total essential amino acids and reduced water solubility, emulsion stability, tannin, and phytate contents compared to raw samh seeds (p < 0.05). The highest levels of pH, ash, carbohydrate, fiber, and glycemic index were observed in raw samh seeds, and both germination and fermentation processes reduced these attributes to various degrees (p < 0.05). Germination increased the redness (a*), moisture content, essential and non-essential amino acids, potassium, zinc, phosphorous, stearic acid, and oleic and unsaturated fatty acids and reduced total solids, fat content, iron, zinc, calcium, magnesium, sodium, palmitic acid, and total saturated fatty acids of the samh seeds compared to the raw ones. Fermentation increased the total solid, acidity, fat, protein, calcium, magnesium, sodium, phosphorous, iron, zinc, palmitic acid, and total saturated fatty acids and reduced the a* value, moisture, non-essential amino acids, and total unsaturated fatty acids of the samh seeds compared to the raw ones. In conclusion, samh seeds are a rich source of nutrients that could generally be enhanced by germination and fermentation processes. The reported information facilitates strategies towards the application of these underutilized seeds in foods.

8.
Ann Med Surg (Lond) ; 85(7): 3634-3637, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37427231

RESUMO

Cardiac tumors are a rare condition that typically presents with nonspecific symptoms. Among the histologic patterns, myxoid sarcomas are rarely identified and may have a less favorable prognosis. Reporting a case of this type of cardiac tumor can increase awareness about this condition and aid in early diagnosis, potentially leading to better outcomes. Case presentation: We are presenting a case of a 41-year-old female with left atrial myxoid sarcoma, which was presented with a cardiogenic shock picture. She underwent surgical excision of the mass and was discharged in good condition. After discharge, she deteriorated and was found to have lung metastases. Clinical discussion: Primary cardiac sarcomas, due to their rarity and poor prognosis, are often diagnosed at an advanced stage of the disease and lack sufficient data to establish a standard course of treatment. The cornerstone of therapy is surgical resection. However, novel therapeutic approaches must be developed. Conclusions: Primary cardiac tumors should be suspected in adult patients with progressive dyspnea, and a biopsy should also be done to determine the histopathological pattern of the mass and estimate the overall prognosis and outcomes.

9.
Cureus ; 14(10): e29864, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36337784

RESUMO

Background This study aims to evaluate the clinical features, laboratory findings, and outcomes of children and adults diagnosed with meningitis in Jordan. Methodology This is a retrospective chart review study that targeted patients diagnosed with meningitis at King Abdullah University Hospital, a tertiary care center in Northern Jordan, from March 21, 2015, to March 31, 2019. Patients were included in this study if they were older than 28 days and had no risk factors for meningitis. Results A total of 169 patients met the inclusion criteria. Males were overrepresented (67%) and were significantly younger than females (6 vs. 17 years, p = 0.01). Positive meningeal signs were not predictive of greater cerebrospinal fluid leukocytosis (p = 0.348), and they did not provide sufficient sensitivity to be used as screening tools. The most common etiology was aseptic (49%), followed by enterovirus (43%), while bacterial meningitis was an uncommon diagnosis (3.5%). Nearly half of the patients took antibiotics prior to their hospital presentation. During in-hospital admission, six patients died, four of whom had bacterial and two had aseptic meningitis. Enteroviral meningitis showed neutrophil predominance in 44% of cases on lumbar puncture and had a higher neutrophil proportion compared to aseptic meningitis (p = 0.026). Streptococcus pneumoniae was the most common bacterial etiology identified. Conclusions Meningitis in Jordan is most commonly of aseptic and enteroviral origin, and these etiologies carry significantly more favorable outcomes compared to bacterial meningitis. Enteroviral meningitis displays a higher percentage of neutrophils in cerebrospinal fluid compared to aseptic meningitis. S. pneumoniae is the leading cause of bacterial meningitis. Slight neutrophil predominance above half is a weak predictor of bacterial meningitis due to the small contribution of bacteria as a cause among enteroviruses and aseptic etiologies.

10.
Cureus ; 14(8): e28236, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-36158333

RESUMO

OBJECTIVES: To highlight the disparity in the regional distribution of neurosurgical workforce in the Kingdom of Saudi Arabia (KSA) and to correlate the provision of neurosurgeons across the regions with several parameters. METHODS:  The 13 administrative emirates of provinces in KSA were grouped into five geographical regions (central, western, eastern, southern, and northern). The density of neurosurgeons was calculated for each region. The distribution of neurosurgeons across the regions was correlated with several parameters using Pearson coefficient test. RESULTS:  This study examined 238 neurosurgeons working in 85 neurosurgical centers in KSA. The regional median (range) density of neurosurgeons was 7.1 (3.1-10.2) per million population and 9.3 (2.3-23.3) per thousand square kilometer area. The regional provision of neurosurgeons correlated significantly with the distribution of KSA-national (p=0.031), KSA-certified (p=0.0004), Government Hospitals (GHs) (p=0.0012), and private hospitals (PHs) (p=0.0359) funded neurosurgeons. The regional allocation of neurosurgeons also correlated positively with the distribution of the total neurosurgical centers (p=0.048), the PHs centers (p=0.0057) but not the GHs centers (p=0.3296). Furthermore, a mismatch was observed between the regional distribution of the neurosurgical workforce and the provision of neurosurgeons according to their GHs' sub-divisions, regional population, and area. CONCLUSIONS:  The regional distribution of neurosurgeons in KSA was uneven. The density of neurosurgeons was the lowest in the southern and northern regions. There was disparity in the number of neurosurgeons employed by the various GHs' sub-divisions and in the allocation of GHs' neurosurgical centers across the regions. Easy access to quality neurosurgical care is imperative. Policy makers should take this into consideration in the future planning of regional neurosurgical services in KSA.

11.
J Nurs Meas ; 30(3): 530-572, 2022 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-33408189

RESUMO

Background and Purpose: Item analysis is important in examining the quality of items. The main aims of this sub-study were to assess and improve the psychometric properties of the modified version of the Routine Blood Transfusion Knowledge Questionnaire (RBTKQ-2) and produce an optimized version (RBTKQ-O) of it. Methods: Statistical item analysis was performed on the RBTKQ-2 after completion by 305 nurses. Results: There were one (3%) easy, 15 (47%) moderately difficult, and 16 (50%) difficult items. Twenty six (81%) items and six (19%) had positive significant and weak point-biserial correlation respectively. The majority of distractors (n = 66, 64%) were functional. Four sources of information, including item analysis data, informed the development of the RBTKQ-O. Conclusions: The RBTKQ-O addressed the limitations that were identified in the versions published earlier. This version can be used by researchers and academics worldwide.


Assuntos
Transfusão de Sangue , Análise Fatorial , Humanos , Psicometria , Reprodutibilidade dos Testes , Inquéritos e Questionários
13.
Cureus ; 13(5): e15313, 2021 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-34211813

RESUMO

Background Palliative care (PC) has a positive effect on symptom burden, quality of life, psychosocial communication, prognostic understanding, mood, and quality of care at the end of life of patients with advanced cancer. Objectives To investigate the timing of the first palliative consultation and referral of advanced cancer patients to the palliative care service and their determinants at King Faisal Specialist Hospital and Research Center (KFSHRC), Jeddah, Saudi Arabia. Subjects and methods A retrospective cohort study was conducted at KFSHRC. It included advanced cancer patients who died between January 1, 2019 and Jun 30, 2020. The dependent variable of primary interest is the timing of PC consultation and the timing of PC referral. The independent variables included age, sex, marital status, nationality, date of death, types of cancer, Eastern Cooperative Oncology Group (ECOG), palliative performance status (PPS), palliative prognostic index (PPI), code status (do not resuscitate [DNR]), the severity of symptoms (assessed by the Edmonton Symptom Assessment System - Revised [ESAS-r]), referral to home health care (HHC), referral to long-term care (LTC), referral to interdisciplinary team (IDT), length of survival after the first PC consultation, length of survival after the referral to the PC service, length of hospital stay, frequency of emergency room (ER) visits and hospital admission in the last year before death, and involvement in bereavement with advanced care planning (ACP) services. Results Of the 210 advanced cancer patients, 109 (51.9%) were male, and their ages ranged between 18 and 90 years. More than half of patients (56.7%) had a history of PC consultation. Among them, PC consultation was described as late in 60.5% of patients. Concerning the timing of palliative care referral among advanced cancer patients, it was too late and much too late among 25.7% and 58.1% of them, respectively. Patients who visited ER more frequently (≥3 times) (p=0.014) and those who referred to HHC (p=0.005) were more likely to consult PC early compared to their counterparts. Length of survival was significantly higher among patients who reported early PC consultation compared to those without PC consultation and those with late PC consultation, p<0.001. Referral to PC for both transfer of care and symptom management was associated with earlier PC consultation, p=0.021. Patients who were admitted to the hospital three times or more were less likely to be much too late referred to PC services, p=0.046. Also, patients who were not referred to long-term care or home health care were more likely to be referred to PC services much too late, p<0.001. Among 28.8% of patients whose PPS ranged between 30% and 50% compared to 14.9% of those whose PPS ranged between 10% and 20% expressed too late referral time to PC, p=0.040. Conclusion In a considerable proportion of terminal cancer patients, palliative care was consulted late, and the timing of palliative care referral was too late/much too late among most of those consulted palliative care. Length of survival was higher among patients who reported early PC consultation and who with ideal referral time to PC services than others. Therefore, future considerations to facilitate early integration of palliative care in cancer patients are highly recommended through mainly improving staff education in communication skills and palliative care approach.

14.
J Magn Reson ; 326: 106934, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33684681

RESUMO

In this paper, we introduce 3D printing as a possibility for realizing lightweight, yet high-precision NMR magnets. Using a commercially available filament containing steel particles allows for the realization of critical components of NMR magnets such as pole pieces and even the flux-conducting yoke. In contrast to shimming structures made of iron, 3D printed structures made of the lightweight filament allow for a robust and inexpensive way of realizing high-performance NMR magnets for future portable NMR applications. We demonstrate the versatility and achievable high performance of the proposed solution with two different H-shaped NMR magnets. In the first magnet, the 3D-printed filament is used to realize the yoke that guides the magnetic flux inside the magnet, providing the potential for a substantial weight reduction compared to a conventional iron yoke. In the second magnet, we use the 3D-printed material to realize arbitrarily shaped passive shim structures. Numerical size and shape optimizations using non-uniform rational basis splines (NURBS) have been applied to obtain the optimal geometry. The two manufactured magnets achieve measured NMR spectral line widths of 54 ppm and 250 ppm, respectively. Our results clearly demonstrate the efficiency and versatility of the proposed design and optimization approach.

15.
Avicenna J Med ; 10(2): 68-75, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32500045

RESUMO

Over the last 5 years, there have been more than 140 new drug approvals in the field of Oncology alone, all based on newly published clinical trials. These approvals have led to an ongoing change in clinical practice, offering new therapeutic options for patients. Therefore, it is important for healthcare providers to be able to appraise a clinical trial and determine its validity, understand its results, and be able to apply such results to their patients. In this guide, we provide a simplified approach tailored to practicing clinicians and trainees. The same concepts and principles apply to other medical specialties.

16.
Crit Care Nurs Q ; 43(3): 274-285, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32433068

RESUMO

The management of critically ill patients is complicated and often involves complex devices including mechanical ventilators (MVs), which may be associated with many complications. Protective lung strategies (PLSs) are used to prevent complications associated with MVs, but nurses may not possess adequate knowledge to optimize the efficacy of PLSs. This article reports findings from what is thought to be the first study in Palestine that explores critical care nurses' knowledge about PLSs. The purpose of this study was to assess the critical care nurses' attitude, behavior, and knowledge regarding PLSs of mechanically ventilated patients. This descriptive, cross-sectional study was conducted at both public and private hospitals in Palestine. The sample included nurses who worked in intensive care units and cardiac care units and utilized a previously developed and validated questionnaire. The result of the study concludes that the majority of the Palestinian critical care nurses agree with the utilization or application of PLSs but have a severe lack of knowledge about the strategies. This highlights the need to provide additional educational programs related to the optimum use of mechanical ventilation.


Assuntos
Atitude do Pessoal de Saúde , Enfermagem de Cuidados Críticos , Conhecimentos, Atitudes e Prática em Saúde , Papel do Profissional de Enfermagem/psicologia , Respiração Artificial/enfermagem , Adulto , Árabes , Estudos Transversais , Feminino , Humanos , Unidades de Terapia Intensiva , Pulmão/fisiopatologia , Masculino , Inquéritos e Questionários
17.
Eur J Clin Microbiol Infect Dis ; 36(6): 993-997, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28161744

RESUMO

In Bangladesh there are several published papers on superficial mycoses. Deep mycoses are also recognized as an important emerging problem. Here, we estimate the annual incidence and prevalence of serious fungal infections in Bangladesh. Demographic data were obtained from world population reports and the data on TB and HIV extracted from the online publications on tuberculosis in Bangladesh and Asia Pacific research statistical data information resources AIDS Data HUB. All the published papers on fungal infections in Bangladesh were identified through extensive search of literature. We estimated the number of affected people from populations at risk and local epidemiological data. Bangladesh has a population of ∼162.6 million, 31% children and only 6% over the age of 60 years. The pulmonary TB caseload reported in 2014 was 119,520, and we estimate a prevalence of 30,178 people with chronic pulmonary aspergillosis, 80% attributable to TB. An anticipated 90,262 and 119,146 patients have allergic bronchopulmonary aspergillosis or severe asthma with fungal sensitization. Only 8,000 people are estimated to be HIV-infected, of whom 2900 are not on ART with a CD4 count <350 µL, Pneumocystis pneumonia and cryptococcal meningitis being rare. Superficial mycoses are very common with Trichophyton rubrum as the predominant etiological agent (80.6%). Numerous cases of mycotic keratitis have been reported from several parts of Bangladesh. Candida bloodstream infection was estimated based on a 5 per 100,000 rate (8100 cases) and invasive aspergillosis based primarily on leukemia and COPD rates, at 5166 cases. Histoplasmosis was documented in 16 cases mostly with disseminated disease and presumed in 21 with HIV infection. This study constitutes the first attempt to estimate the burden of several types of serious fungal infections in Bangladesh.


Assuntos
Micoses/epidemiologia , Micoses/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Bangladesh/epidemiologia , Criança , Pré-Escolar , Comorbidade , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Prevalência , Medição de Risco , Adulto Jovem
18.
Opt Lett ; 40(10): 2237-40, 2015 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-26393708

RESUMO

We report the fabrication of a tellurite optical fiber with a suspended core design, formed on a 220-nm-wide filament of glass. The fiber was pumped at two different wavelengths (1500 and 2400 nm) using femtosecond pulses generated from an optical parametric oscillator (OPO) in order to produce mid-infrared supercontinuum (SC). We observed that SC spectra extending to 3 µm were readily generated. To further optimize the design, detailed numerical study was performed, which revealed how the fiber structural characteristics dramatically influence the spectral broadening because of the changes in the dispersion profile and in turn, the interplay of nonlinear effects that give rise to SC generation. We found that an accurate control of the core shape can be employed to contain the generated SC spectra within well-defined spectral regions or to provide a broad extension of the continuum to beyond 4 µm.

19.
Naunyn Schmiedebergs Arch Pharmacol ; 388(12): 1271-82, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26264806

RESUMO

The antitumor activity of carbonic anhydrase (CA) inhibitors is attributed to their ability to induce a state of intracellular acidification. In fact, acidic intracellular pH was demonstrated to upregulate several tumor suppressor proteins and increase the activity of many chemotherapies. The present study aimed to investigate the antitumor activity of the CA inhibitor, dorzolamide, in combination with mitomycin C and to study the effect of these drugs on tumoral thioredoxin-interacting protein (TXNIP) as well as tumor cell proliferation and apoptosis. Solid tumors were induced by subcutaneous inoculation of Ehrlich's ascites carcinoma (EAC) cells in female mice. Mice were treated with dorzolamide (3, 10, or 30 mg/kg/day, i.p.) and/or mitomycin C (1 mg/kg, i.p.) weekly for 3 weeks. Treatment with mitomycin C increased TXNIP level in EAC solid tumors in mice. Likewise, treatment with dorzolamide upregulated TXNIP and p53 while downregulated bcl-2. Both drug therapies increased tumoral caspase 9, caspase 3, and PARP-1 cleavage in addition to decreasing the proliferative Ki-67-stained nuclear fraction. Indeed, a synergistic effect was detected between mitomycin C and dorzolamide. The current data demonstrated that the antitumor activity of mitomycin C and dorzolamide was, at least in part, mediated through stimulating tumoral expression of TXNIP and enhancing tumor apoptosis.


Assuntos
Antibióticos Antineoplásicos/uso terapêutico , Carcinoma de Ehrlich/tratamento farmacológico , Proteínas de Transporte/fisiologia , Mitomicina/uso terapêutico , Sulfonamidas/uso terapêutico , Tiofenos/uso terapêutico , Tiorredoxinas/fisiologia , Animais , Carcinoma de Ehrlich/patologia , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Feminino , Camundongos
20.
Opt Express ; 22(9): 10544-9, 2014 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-24921756

RESUMO

We report the first demonstration of an inline few-mode thulium doped fiber amplifier (TDFA) operating at 2µm for mode division multiplexed transmission. Similar gain and noise figure performance for both the LP(01) and LP(11) modes are obtained in a cladding pumped 2-mode group TDFA. A maximum gain of 18.3dB was measured at 1970nm with a 3dB gain bandwidth of 75nm while the average noise figure was measured to be between 7 and 8dB for wavelengths longer than 1970nm.

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