[The migration activity of leukocytes as indicator of hyper-sensitivity of delayed type in patients with erysipelas.]

The hyper-sensitivity of delayed type is one of important manifestations of cellular immunity. The successful studying of this phenomenon can favor solution of actual issues of pathogenesis, diagnostics and treatment of bacterial infections. The present review considers hyper-sensitivity of delayed type as an important area of cellular immunity under infectious pathology and macrophage-inhibiting factor as an important lymphocyte mediator: cytokine and hormone. The model of intestinal infections and erysipelas was used to demonstrate an important diagnostic and prognostic value of screening test of cellular migration as a simple, handy in application and reading of results, relatively new technique permitting to evaluate both acceleration of migration of leukocytes at height of disease and inhibition of migration in re-convalescence of disease. The establishment of character and dynamics of migration activity of leukocytes on partial genes antigens S.pyogenes in screening test of cellular migration can be used in diagnostics of various forms of erysipelas, evaluation of expression of intoxication, prognosis of complications and relapses. The disturbance of dynamics of migration activity of leukocytes testifies failure of immune response to agent.

[IMMUNOLOGIC MONITORING OF HELICOBACTER PYLORI PERSISTENCE IN THE ORGANISM].

Generalized results of 15-year prospective studies of frequency of occurrence and dynamics of circulation of pathogenetically significant LPS/O-antigens, high molecular weight proteins, including CagA, and VacA of Helicobacter pylori in biological media of organism in patients with gastrointestinal diseases and asymptomatic volunteers due to effects of external and internal factors are presented. Features of antigen circulation and reciprocal immune reaction of the organism are established, that reflect their interaction in the parasite-host tandem, risk and prognosis of possible complications in the process of long-term persistence of Helicobacter pylori in the organism.

Diagnostic significance of the coagglutination reaction in patients with the diarrhoea syndrome.

Specific soluble Shigella, Salmonella and Yersinia enterocolitica antigens were determined in biological fluids (saliva, urine, coprofiltrate) from 268 patients with the diarrhoea syndrome using the coagglutination reaction. The findings suggest that the coagglutination reaction (COA) is a simple and efficient method suitable for the fast diagnosing of acute intestinal infection (AII) in the early days from the onset of the disease. COA enables the identification of specific antigens associated with the causative agents of intestinal infections in 79-54% of patients with shigellosis and salmonellosis. COA was shown to possess a high diagnostic potential in AII of unknown etiology. Shigella, Salmonella and Yersinia antigens were determined in 47.7, 23.4 and 10.8% of cases respectively where no bacterial excretion could be confirmed. Two and less frequently three antigens being identified simultaneously in 10.8% cases. The identification of opportunistic microorganisms in AII using the COA does not appear to be sufficient to confirm their etiological significance as Shigella and Salmonella antigens were simultaneously determined in most patients.