[Analysis of the spectrum of bacterial pathogens isolated from patients with complicated skin and soft tissue infections presumably due to grampositive or mixed flora in the countries of the Central and East Europe].

The data on the microbiological investigation of clinical materials from patients with complicated skin and soft tissue infections in 6 European countries were analysed. The analysis of the bacterial pathogens spectrum provided the microbial view and efficient use of novel antimicrobials in clinical trials.

[Photo-hemo-correction in the complex treatment of patients with obliterating atherosclerosis of the arteries of the lower limbs]. / Fotogemokorrektsiia v kompleksnom lechenii bol'nykh s obliteriruiushchim aterosklerozom arterii nizhnikh konechnostei.

Based on the assessment of clinical efficiency of the intravascular and percutaneous photomodification of blood with a helium-neon laser in 170 patients the authors have shown that it is expedient to include the photohemocorrection in the medical programs for the I-III degree ischemia.

[Feasibility of correction of blood aggregation properties in the course of extracorporeal hemosorption in atherosclerosis patients]. / Vozmozhnosti korrektsii agregatnogo sostoianiia krovi pri provedenii ékstrakorporal'noi gemokorrektsii bol'nym aterosklerozom.

Aggregation and rheological properties of blood were evaluated in 20 atherosclerotic patients after cryoplasmosorption. The change of the patients' plasma for extracorporeally modified autoplasma reduced coagulation potential, enhanced fibrinolysis and suppressed anticoagulation systems. Clinical efficacy manifested in decreased frequency and duration of anginal episodes, intensity of intermittent claudication. Biochemically, cholesterol levels and atherogenic index lowering was evident.

[Cryohemosorption--a method of removal of lipoprotein-antibody immune complexes and other atherogenic substances in patients with atherosclerosis]. / Krioplazmosorbtsiia--metod udaleniia immunnykh kompleksov lipoproteid-antitelo i drugikh aterogennykh substantsii u bol'nykh aterosklerozom.

The efficacy of extracorporeal cryohemosorption was estimated in the treatment of atherosclerotic patients in terms of the autoimmune theory of the pathogenesis of this disease. There was a slight decrease in the plasma levels of total cholesterol and triglycerides, there was over 2-fold reduction in the plasma levels of fibrinogen and fibronectin. All major components of apo B-containing lipoproteins and immunoglobulin G were found as part of the cryoprecipitate. An extremely high concentration of lipid hydroperoxides in the precipitate suggest that cryoprecipitation removes not only autoimmune complexes, but highly-atherogenic peroxide-modified lipoproteins. The three-fold decrease in the levels of lipoprotein-antibody complexes resulted in lower atherogenicity of apo B-containing lipoproteins. It is suggested that the mechanism responsible for beneficial clinical action of cryohemosorption sessions is largely associated with the removal of autoimmune lipoprotein-antibody complexes and peroxide-modified apo B-containing lipoproteins than correction of lipid metabolism.

[Effects of plasmapheresis on the activity of lipid peroxidation in peritonitis]. / Vliianie plazmafereza na aktivnost' protsessov perekisnogo okisleniia lipidov pri peritonite.

The destructive process in the abdominal cavity leading to peritonitis is accompanied by the activation of free-radical lipid oxidation which promotes the disturbances of cellular membrane integrity and the development of endotoxemia. Hemodilution and plasmapheresis decreased the blood level of lipid peroxidation products and activated the system of antioxidant protection.

[The storage and preparation of autologous plasma for transfusions to patients with arteriosclerosis obliterans of the vessels of the lower extremities]. / O khranenii i podgotovke autoplazmy k transfuziiam bol'nym obliteriruiushchim aterosklerozom sosudov nizhnikh konechnostei.

An investigation of the regimen of storage and sorption of plasma in patients with obliterating atherosclerosis of lower extremity vessels has shown that the optimum regimen of storage of plasma is freezing (with addition of 1 thousand of heparin units per 100 ml of plasma) at t = -20 degrees C followed by thawing at t = +6(+/- 2) degrees C in running water before sorption. The optimal sorbent for sorption of plasma of patients with obliterating atherosclerosis of lower extremity vessels is "Actilen" charcoal. The optimal method of sorption is sorption after thawing the plasma. The sufficient degree of plasma purification can be reached in the ratio sorbent/sorbate for charcoal "Actilen" equal to 1:100 and a single perfusion of plasma through the column.

[Endogenous intoxication syndrome in patients in late stages of obliterating atherosclerosis in vessels of lower extremities]. / Sindrom éndogennoi intoksikatsii u bol'nykh pozdnimi stadiiami obliteriruiushchego ateroskleroza sosudov nizhnikh konechnostei.

The composition of blood was evaluated (parameters of blood clinical analysis, figures of lipid peroxidation processes and system of antioxidant protection, traditional biochemical blood parameters, total proteolytic activity and peptides of blood plasma, quantity and activity of some parameters of humoral immunity) in patients with late stages of peripheral atherosclerosis. It was showed that chronic endotoxicosis appeared in these patients. The processes of chronic endotoxicosis are compensated in patients with the 3-d stage of low extremities ischemia. The quantity of endogenic toxins and markers of endogenic intoxication increased in patients with the 4-th stage of ischemia and loading on the system of detoxication was enlarged; and as a result, compensation reserves of detoxication system of organism decreased.

[Response of the pro- and antioxidant systems to plasmapheresis in patients with advanced atherosclerosis obliterans of the legs]. / Reaktsiia pro- i antioksidantnykh sistem na provedenie plazmafereza u bol'nykh obliteriruiushchim aterosklerozom sosudov nizhnikh konechnostei pozdnikh stadii.

The effects of fractional apparatus plasmapheresis in a volume of 25% of circulating blood plasma on the processes of lipid peroxidation and on the antioxidant defence system parameters were studied in 31 patients with ischemia of the lower limbs, stage III-IV. It has been shown that plasmapheresis induces elevation of ceruloplasmin activity and superoxide dismutase activity of blood plasma, lowers the ratio of lipid oxidation to lipid peroxidation products simultaneously with intensification of lipid peroxidation that leads to regeneration of biological membranes of the body.

[The effect of hemosorption on the activity of free radical oxidation in destructive diseases of the abdominal cavity organs]. / Vliianie gemosorbtsii na aktivnost' svobodnoradikal'nogo okisleniia pri destruktivnykh zabolevaniiakh organov briushnoi polosti.

Depletion of antioxidant system which accompanies excessive formation of oxygen radicals and increased activity of lipid peroxidation in destructive diseases of the abdominal organs leads to the generalization of the pathological process and severe endogenous intoxication. Hemosorption as part of intensive therapy in patients with peritonitis ensures a decrease in endotoxemia degree due to elimination of toxic LPO metabolites from the blood flow and stabilizes in the post-hemosorption period the activity of free radical reactions due to an increase in antioxidant activity.

[The role of lipid peroxidation processes in the development of complications following reconstructive surgery on the vessels of the lower extremities]. / Rol' protsessov perekisnogo okisleniia lipidov v razvitii oslozhnenii posle rekonstruktivnykh operatsii na sosudakh nizhnikh konechnostei.

Reconstructive surgery on the vessels of the lower extremities accompanied by ischemia and the following tissue oxygenation leads to the activation of lipid peroxidation causing the destruction of cellular membranes and formation of toxic products, namely medium mass oligopeptides. The accumulation of lipid peroxidation metabolites in the blood correlates with the onset of renal-hepatic failure complicating the postoperative period.

[The role of the activation of lipid peroxidation in the pathogenesis of experimental peritonitis]. / Rol' aktivatsii perekisnogo okisleniia lipidov v patogeneze éksperimental'nogo peritonita.

Investigation was carried out for studying the role of lipid peroxidation (LP) in the genesis of destructive process in the abdominal cavity. The model of experimental peritonitis was made on the dogs. According to the concentration of fat acids conjugates with double couplings (diene conjugates--DC), malondialdehyde (MDA) the LP activity was discovered. Simultaneously the activity of ceruloplasmin (CP), superoxide dismutase activity of plasma, total proteolytic blood activity, summary indicators of antioxidants plasma status and the level of medium-mass molecules was indicated. It was established that LP activation began in the first hours of peritonitis development yet, concentration of LP metabolites (MDA and DC) in plasma increased during disease progressing. CP and superoxide dismutase activity of plasma decreased. Total proteolytic blood activity and level of medium-mass molecules increased after the growth of LP intensity.

[Increase in the effectiveness of microbial absorption on activated charcoal upon sorbent polarization]. / Povyshenie éffektivnosti sorbtsii mikroorganizamov na aktivirovannom ugle pri poliarizatsii sorbenta.

The effect of Staphylococcus albus and the contact time on hydrolytic adsorption of ions on activated charcoal SKN-2K in the physiological solution (0.9% NaCl) was investigated. Experiments were performed without polarization or with cathodic and anodic polarization of the adsorbent. The adsorbed microorganisms decreased the rate of electrolyte ion adsorption, especially in case of cathodic polarization. When activate charcoal was at negative potential (-0.2 V), up to 95% of active microorganisms was adsorbed at the initial concentration of 5.75.10(6) microorganisms per ml, while in case of anodic polarization only 43-44% of microorganisms was adsorbed.