A simple and robust LC-ESI single quadrupole MS-based method to analyze polyphenols in plant extracts using deep eutectic solvents.

Currently, the interest in polyphenols is increasing due to their significant properties in health. Polyphenols exist in a range of natural products, however their extraction as well as their characterization are important issues as they are mainly present in complex matrices. Therefore, sensitive and selective analytical methods based on liquid chromatography coupled to tandem mass spectrometry are essential. Nevertheless, access to such high-resolution techniques is quite rare. Thus, in this work we present a simple, selective and robust method based on a single-quadrupole (Q) MS technique) for the analysis of a wide range of polyphenols such as flavonoids, phenolic acids and anthocyanins. Specifically, we present:•A simple liquid chromatography electro-spray ionization (LC-ESI) single-quadrupole mass selective (MS) method for the analysis of 18 different polyphenols.•Application of the method to three plant-based extracts that are derived after green extraction methods.

Antioxidants and Bioactive Compounds in Food: Critical Review of Issues and Prospects.

This review paper gives an insight into the effective delivery mechanisms for health-promoting substances and highlights the challenges of using antioxidants and bioactives in foods. The selection criteria for choosing bioactives and their extraction in bioavailable form with their adequate incorporation techniques and delivery mechanisms are covered. Moreover, an overview of existing methods for determination of bioactivity is given. The importance of scientifically evaluating the effects of foods or food components on consumer health before making claims about the healthiness is aligned. Finally, a scientific perspective on how to respond to the booming demand for health-promoting products is given, and we acknowledge that despite the work done, there are still many challenges that need to be overcome.