RESUMO
A radioimmunoassay determination method was developed for 17-epimethyltestosterone (17 alpha-hydroxy-17-methyl-4-androstein-3-one). Excretion of metabolites during and after methandienone (17 beta-hydroxy-17-methyl-1,4-androstadien-3-one) administration was followed in human urine samples by RIA tests for methandienone and 17-epimethyltestosterone. While alternating peaks were found in both measured excretion curves, their addition results in a normal curve showing a plateau between the 3rd and 6th day of the drug administration. Furthermore, due to the presence of higher amounts of epi-configurated metabolites, the new test has a higher effectiveness in the detection of the metabolites.
Assuntos
Metandrostenolona/urina , Metiltestosterona/urina , Animais , Reações Cruzadas , Masculino , Coelhos , Radioimunoensaio/métodosAssuntos
Pseudouridina/urina , Uridina/análogos & derivados , Animais , Pseudouridina/sangue , Ratos , Resorcinóis , EspectrofotometriaRESUMO
Daily urinary excretion of pseudouridine, creatinine and creatine of chronically irradiated Wistar rats was estimated. The irradiation conditions were: 60Co gamma source, dose-rate 10 rad/day, total dose 200, 400 and 600 rad. Control groups were kept under similar conditions. Urine samples were taken three times after the end of the irradiation period. It was found that: (1) pseudouridine excretion seems more suitable for indicating radiation injury than the creatine/creatinine ratio in chronic irradiation in rats; (ii) there are significant changes in dose dependence of pseudouridine excretion in the post-irradiation period; (iii) a new method for pseudouridine estimation gives closely similar data to those of earlier investigations.
