Elucidating the polycyclic aromatic hydrocarbons involved in soot inception.

Polycyclic aromatic hydrocarbons are the main precursors to soot particles in combustion systems. A lack of direct experimental evidence has led to controversial theoretical explanations for the transition from gas-phase species to organic soot clusters. This work focuses on sampling infant soot particles from well-defined flames followed by analysis using state-of-the-art mass spectrometry. We found that PAH molecules present in soot particles are all stabilomers. Kinetic Monte Carlo simulations and thermodynamic stability calculations further identify the detected PAHs as peri-condensed and without aliphatic chains. Van der Waals forces can easily link PAHs of such size and shape to form PAH dimers and larger clusters under the specified flame conditions. Our results provide direct experimental evidence that soot inception is initiated by a physical process under typical flame conditions. This work improves our understanding of aerosol particulates, which has implications for their environmental and climate change impacts.

Bullseye dielectric cavities for photon collection from a surface-mounted quantum-light-emitter.

Coupling light from a point source to a propagating mode is an important problem in nano-photonics and is essential for many applications in quantum optics. Circular "bullseye" cavities, consisting of concentric rings of alternating refractive index, are a promising technology that can achieve near-unity coupling into a first lens. Here we design a bullseye structure suitable for enhancing the emission from dye molecules, 2D materials and nano-diamonds positioned on the surface of these cavities. A periodic design of cavity, meeting the Bragg scattering condition, achieves a Purcell factor of 22.5 and collection efficiency of 80%. We also tackle the more challenging task of designing a cavity for coupling to a low numerical aperture fibre in the near field. Finally, using an iterative procedure, we study how the collection efficiency varies with apodised (non-periodic) rings.

Coherence protection of spin qubits in hexagonal boron nitride.

Spin defects in foils of hexagonal boron nitride are an attractive platform for magnetic field imaging, since the probe can be placed in close proximity to the target. However, as a III-V material the electron spin coherence is limited by the nuclear spin environment, with spin echo coherence times of ∽100 ns at room temperature accessible magnetic fields. We use a strong continuous microwave drive with a modulation in order to stabilize a Rabi oscillation, extending the coherence time up to ∽ 4µs, which is close to the 10 µs electron spin lifetime in our sample. We then define a protected qubit basis, and show full control of the protected qubit. The coherence times of a superposition of the protected qubit can be as high as 0.8 µs. This work establishes that boron vacancies in hexagonal boron nitride can have electron spin coherence times that are competitive with typical nitrogen vacancy centres in small nanodiamonds under ambient conditions.

Training and Match Demands of Elite Rugby Union.

ABSTRACT: Cousins, BEW, Morris, JG, Sunderland, C, Bennett, AM, Shahtahmassebi, G, and Cooper, SB. Training and match demands of elite rugby union. J Strength Cond Res 37(1): 141-148, 2023-This study aimed to examine training and match demands associated with elite Rugby Union. Eighty-nine elite players were monitored using subjective (session ratings of perceived exertion) and objective (global positioning systems: distance and high-speed running [defined as >70% of individual maximum speed] distance) methods, alongside key performance indicator variables in matches (e.g., number of tackles made). These were compared between positions (forwards vs. backs) and league of competition (Premiership vs. Championship). Statistical significance was accepted as p < 0.05. Analysis revealed that backs covered greater distance (by 704 m, p < 0.001) in training and greater distance (by 7.6 m·min-1, p < 0.001) and high-speed running distance (by 1.22 m·min-1, p < 0.001) in matches, compared with forwards. In matches, the forwards experience greater key performance indicator demand (tackles: 78%; tackle assists: 207%; breakdown entries: 324%; contact events: 117%; all p < 0.001) compared with backs. The number of tackles (53%, p < 0.001) and tackles missed (35%, p = 0.001) were greater, whereas contact carries (12%, p = 0.010) and breakdown entries (10%, p = 0.024) were lower, in the Premiership compared with the Championship. Overall, these findings confirm that the running demands of Rugby Union are higher in backs, whereas contact actions are higher in forwards, with further differences between the Premiership and Championship. This comprehensive examination of the demands of elite Rugby Union could be used to ensure the specificity of training protocols for elite Rugby Union clubs, specific to both playing position (forward or back) and level of competition (Premiership or Championship).

Synthetic playing surfaces increase the incidence of match injuries in an elite Rugby Union team.

OBJECTIVES: To examine differences in match injury incidence between three playing surfaces in elite Rugby Union. DESIGN: Prospective cohort. METHODS: Match injury incidence was assessed in 89 elite Rugby Union players over two-seasons of professional competition (44 matches, 1014 h player exposure). Match injury incidence was assessed on three different playing surfaces; natural grass, hybrid (natural grass combined with approximately 3% synthetic fibres) and fully synthetic (sand and rubber infill). Overall injury incidence, contact and non-contact injury incidence, and the incidence of minor (≤7 d lost) and major (≥8 d lost) injuries were considered using mixed effect models. RESULTS: Overall match injury incidence doubled on hybrid and synthetic surfaces compared to natural grass (hybrid: OR = 2.58 [95% CI 1.65-4.03], p < 0.001; synthetic: OR = 2.16 [95% CI 1.07-4.37], p = 0.033). Furthermore, the odds of sustaining a contact injury on a pitch containing any synthetic content also increased compared to natural grass (hybrid: OR = 2.31 [95% CI 1.41-3.78], p = 0.001; synthetic: OR = 2.19 [95% CI 1.00-4.77], p = 0.049). The hybrid surface elicited a four times greater likelihood of non-contact injury incidence compared to natural grass (OR = 4.18 [95% CI 1.16-15.04], p = 0.028). However, the playing surface did not affect the severity of match injuries (all p > 0.05). CONCLUSIONS: The present study suggests that even a small percentage (3%) of synthetic content in the playing surface significantly increases match injury incidence, with an effect seen on both contact and non-contact injury incidence. These findings are important to enable practitioners to be aware of the injury implications of playing matches on hybrid and synthetic pitches.

Augmenting an online self-directed intervention for gambling disorder with a single motivational interview: study protocol for a randomized controlled trial.

BACKGROUND: Despite the success of gold standard cognitive-behavioral therapy for problem and disordered gambling, the majority of individuals with gambling problems do not seek or receive professional treatment. Thus, the development of less intrusive self-directed interventions has been encouraged. Bibliotherapy for problem gambling has shown promise, both alone and in combination with motivational interviews, but there is still a lack of online self-directed intervention research. The current randomized controlled trial proposes to assess the additive benefit of a single digital motivational interview delivered in conjunction with an online self-directed treatment program for problem gambling and gambling disorder. METHODS: A two-arm randomized controlled trial will be conducted, wherein eligible participants (N=270) will be recruited across Canada via internet advertisements posted to several platforms. All participants will receive access to an online self-directed gambling intervention program. Participants will be randomly assigned to either complete the online program alone or receive a digital motivational interview, conducted through an online audioconferencing platform (i.e., Microsoft Teams) to supplement the online program. The primary outcomes of gambling severity, frequency, and expenditures will be tracked along with secondary outcomes (i.e., depression, anxiety, general distress, alcohol use, and online program user data) over a 24-month period. It is expected that participants in both groups will experience a reduction in symptoms across the board, but more substantial improvements will be observed in the group that receives a supplemental motivational interview. DISCUSSION: The results of this trial will expand upon prior gambling intervention research by informing best practices for the provision of online self-help for problem gambling. TRIAL REGISTRATION: ISRCTN ISRCTN13009468 . Registered on 7 July 2020.

Tailoring topological edge states with photonic crystal nanobeam cavities.

The realization of topological edge states (TESs) in photonic systems has provided unprecedented opportunities for manipulating light in novel manners. The Su-Schrieffer-Heeger (SSH) model has recently gained significant attention and has been exploited in a wide range of photonic platforms to create TESs. We develop a photonic topological insulator strategy based on SSH photonic crystal nanobeam cavities. In contrast to the conventional photonic SSH schemes which are based on alternately tuned coupling strength in one-dimensional lattice, our proposal provides higher flexibility and allows tailoring TESs by manipulating mode coupling in a two-dimensional manner. We reveal that the proposed hole-array based nanobeams in a dielectric membrane can selectively tailor single or double TESs in the telecommunication region by controlling the coupling strength of the adjacent SSH nanobeams in both transverse and axial directions. Our finding provides an additional degree of freedom in exploiting the SSH model for integrated topological photonic devices and functionalities based on the well-established photonic crystal nanobeam cavity platforms.

Room-Temperature Quantum Emitter in Aluminum Nitride.

A device that is able to produce single photons is a fundamental building block for a number of quantum technologies. Significant progress has been made in engineering quantum emission in the solid state, for instance, using semiconductor quantum dots as well as defect sites in bulk and two-dimensional materials. Here we report the discovery of a room-temperature quantum emitter embedded deep within the band gap of aluminum nitride. Using spectral, polarization, and photon-counting time-resolved measurements we demonstrate bright (>105 counts s-1), pure (g (2)(0) < 0.2), and polarized room-temperature quantum light emission from color centers in this commercially important semiconductor.

Match and Training Load Exposure and Time-Loss Incidence in Elite Rugby Union Players.

OBJECTIVE: To investigate the impact of match and training load on time-loss incidence in elite, professional Rugby Union players. MATERIALS AND METHODS: Eighty-nine Rugby Union players were monitored over two seasons of training and competition. Load was measured for all training sessions and matches using subjective [session ratings of perceived exertion (sRPE) load; RPE × session duration] and objective [global positioning systems (GPS); distance and high-speed running distance] methods and quantified using multiple approaches; absolute match and training load, acute:chronic workload ratio (ACWR), exponentially weighted moving average (EWMA) and cumulative 7, 14, 21, and 28 d sums. Mixed effect models were used to assess the effect of each variable on time-loss incidence. RESULTS: Of the 474 time-loss incidences that occurred across the two seasons, 50.0% were contact injuries (86.5% occurred during matches and 13.5% during training), 34.8% were non-contact injuries (31.5% occurred during matches and 68.5% during training) and 15.2% were cases of illness. The absolute match and training load variables provided the best explanation of the variance in time-loss incidence occurrence [sRPE load: p < 0.001, Akaike information criterion (AIC) = 2936; distance: p < 0.001, AIC = 3004; high-speed running distance: p < 0.001, AIC = 3025]. The EWMA approach (EWMA sRPE load: p < 0.001, AIC = 2980; EWMA distance: p < 0.001, AIC = 2980; EWMA high-speed running distance: p = 0.002, AIC = 2987) also explained more of the variance in time-loss incidence occurrence than the ACWR approach (ACWR sRPE load: p = 0.091, AIC = 2993; ACWR distance: p = 0.008, AIC = 2990; ACWR high-speed running distance: p = 0.153, AIC = 2994). CONCLUSION: Overall, the absolute sRPE load variable best explained the variance in time-loss incidence, followed by absolute distance and absolute high-speed running distance. Whilst the model fit using the EWMA approach was not as good as the absolute load variables, it was better than when the same variables were calculated using the ACWR method. Overall, these findings suggest that the absolute match and training load variables provide the best predictors of time-loss incidence rates, with sRPE load likely to be the optimal variant of those examined here.

Does providing a brief internet intervention for hazardous alcohol use to people seeking online help for depression reduce both alcohol use and depression symptoms among participants with these co-occurring disorders? Study protocol for a randomised controlled trial.

INTRODUCTION: Hazardous alcohol consumption is common among people experiencing depression, often acting to exacerbate depressive symptoms. While many people with these co-occurring disorders do not seek face-to-face treatment, they do seek help online. There are effective internet interventions that target hazardous alcohol consumption or depression separately but none that combine these online interventions without the involvement of a therapist. In order to realise the potential of internet interventions, we need to develop an evidence base supporting the efficacy of internet interventions for co-occurring depression and hazardous alcohol use without any therapist involvement. This study aims to evaluate the effects on drinking, and on depressive symptoms, of combining an internet intervention targeting hazardous alcohol consumption with one for depression. METHODS AND ANALYSIS: A double blinded, parallel group randomised controlled trial will be used. Participants with current depression who also drink in a hazardous fashion (n=986) will be recruited for a study to 'help improve an online intervention for depression'. Participants will be randomised either to receive an established online intervention for depression (MoodGYM) or to receive MoodGYM plus a brief internet intervention for hazardous alcohol consumption (Check Your Drinking; CYD). Participants will be contacted 3 and 6 months after receiving the interventions to assess changes in drinking and depression symptoms. It is predicted that participants receiving the CYD intervention in addition to MoodGYM will report greater postintervention reductions in alcohol consumption and depressive symptoms compared with those who received MoodGYM only. Hypothesised mediation and moderation effects will also be investigated. Using an intention-to-treat basis for the analyses, the hypotheses will be tested using a generalised linear hypothesis framework, and longitudinal analyses will use either generalised linear mixed modelling or generalised estimating equation approach where appropriate. ETHICS AND DISSEMINATION: This research comprises the crucial first steps in developing lower-cost and efficacious internet interventions for people suffering from depression who also drink in a hazardous fashion-promoting the widespread availability of care for those in need. This study has been approved by the standing ethics review committee of the Centre for Addiction and Mental Health, and findings will be disseminated in the form of at least one peer-reviewed article and presentations at conferences. TRIAL REGISTRATION NUMBER: NCT03421080; Pre-results.

Online interventions for problem gamblers with and without co-occurring problem drinking: study protocol of a randomized controlled trial.

BACKGROUND: The current randomized controlled trial seeks to evaluate whether providing access to an Internet intervention for problem drinking in addition to an Internet intervention for problem gambling is beneficial for participants with gambling problems who do or do not have co-occurring problem drinking. METHODS: Potential participants will be recruited online via a comprehensive advertisement strategy, if they meet the criteria for problem gambling. As part of the baseline measures, problem drinking will also be assessed. Eligible participants (N = 280) who agree to partake in the study and to be followed up for 6 months will be randomized into one of two versions of an Internet intervention for gamblers: an intervention that targets only gambling issues (G-only) and one that combines a gambling intervention with an intervention for problem drinking (G + A). For problem gamblers who exhibit co-occurring problem drinking, it is predicted that participants who are provided access to the G + A intervention will demonstrate a significantly greater level of reduction in gambling outcomes at 6 months compared to those provided access to the G-only intervention. DISCUSSION: This trial will expand upon the current research on Internet interventions for addictions and inform the development of treatments for those with co-occurring problem drinking and gambling. TRIAL REGISTRATION: ClinicalTrials.gov, NCT03323606 . Registered on 24 October 2017.

Outcomes of an online computerized cognitive behavioral treatment program for treating chinese patients with depression: A pilot study.

OBJECTIVE: This pilot study examined the feasibility, safety, and effectiveness of using an online computerized cognitive behavioral therapy (CBT) for treating Chinese patients with depression. METHODS: Seventy-five Chinese patients with depression in outpatient clinics were randomized into a 5-week intervention. The intervention group received the Chinese translated version of MoodGYM in addition to usual treatment, and the control group continued with usual treatment. Participants completed the 20-item Center for Epidemiologic Studies Depression Scale (CES-D) pre- and post-intervention. RESULTS: We conducted multivariate linear regression analyses to compare the change in CES-D scores for completers after the intervention. Seventy-eight percent (n=29) of participants in the intervention group and 84% (n=32) of patients in the control group completed the post-treatment assessments, and no serious adverse events were reported. Results indicated that while both groups significantly improved at post test, the intervention group improved significantly more than the control group (t(59)=2.37, p=0.02). CONCLUSION: Computerized CBT can be a cost-effective adjunct to medication treatment, particularly in areas with limited access to mental health services. The use of online computerized CBT has been found to be effective in many studies in Western countries, but has not been studied among Chinese outpatients. This study suggests that online computerized CBT is acceptable, and that MoodGYM is a feasible and efficacious augmentative treatment, specifically when used within an outpatient clinic population. With this small sample size we were able to demonstrate that the addition of MoodGYM to usual care improved treatment outcomes for outpatients with depression in China.

Effectiveness and cost-effectiveness of a fully self-guided internet-based intervention for sub-clinical social anxiety symptoms: Protocol for a randomised controlled trial.

DESIGN AND OBJECTIVE: This paper describes the protocol for a large-scale pragmatic, randomised controlled trial and economic evaluation to investigate the effectiveness and cost-effectiveness of the self-directed E-Couch social anxiety module versus a waiting list control condition, for reducing sub-clinical social anxiety symptoms in the general population. STUDY POPULATION: Community-based adults (aged 18+) with social anxiety symptoms that do not meet the criteria for social anxiety disorder recruited via a direct-to-consumer advertisement on national websites. INTERVENTION AND CONTROL: Intervention is the self-guided E-Couch social anxiety module. Control group participants are placed on a waiting list to receive the intervention at the end of the trial. Both groups receive email and text message reminders. OUTCOME MEASURES: The primary outcome will be change in self-reported social anxiety score using the Social Phobia Inventory (SPIN). Secondary outcomes will be the changes in the following self-report measures: Brief Fear of Negative Evaluation scale (BFNE-S); depression (CES-D); mental wellbeing (SWEMWEBS); health status (SF36); use of health services; safety events; and adherence, retention, and attrition rates. All measures will be administered at baseline, 6 weeks, and 3, 6 and 12 months. ANALYSIS: A mixed effects model will be used to analyse the effect of the intervention on the primary and secondary outcomes (intention to treat analysis). Secondary analyses will explore moderators and mediators of effect. A prospective economic evaluation, conducted from a NHS and social care perspective, will provide estimates of cost utility and cost-effectiveness. An interview study will be conducted with 20 participants to explore issues including acceptability, adherence, retention and attrition. TRIAL REGISTRATION NUMBERS: NCT02451878 and ISRCTN15819951.

User characteristics and usage of an open access moderated internet support group for depression and other mental disorders: A prospective study.

BACKGROUND: Internet support groups (ISGs) for mental ill-health are common but little is known about the characteristics of users, the usage and predictors of ISG usage and if and how these change over time. AIM: This study evaluated the attributes of a publically accessible ISG for depression and other mental disorders including: (1) the demographic and other characteristics of its users; (2) their patterns of usage; and (3) the factors which predict posts to and retention on the ISG. METHOD: User characteristics (gender, age, user type, country and location of residence) were collected at the time of registration on the ISG BlueBoard (blueboard.anu.edu.au). All board log data were downloaded for the period October 2008 to May 2014. Predictors of post frequency and retention on the board were examined using logistic regressions. Other data were analysed using descriptive statistics. RESULTS: 2932 users contributed 131,004 posts to the ISG. The majority were female, aged 20 to 34 years, and mental health consumers. Although most users were city dwellers, 19% resided in rural or remote regions. Frequency of posts and retention on the board varied across users, with a moderate association between retention and number of posts. Growth in posts substantially exceeded the growth in new users over the monitoring period. Multivariate analysis demonstrated that consumers posted more often and remained longer than carers or others, and that younger users posted less often; however, the model predicted very little of the variance. CONCLUSIONS: A small minority of active users are sufficient to ensure the sustainability and growth of an online mental health ISG. Further research is required to understand why so many support group members limit their contributions to one or a very small number of posts and what factors predict and promote active engagement and long-term retention in virtual mental health communities.

What's all the talk about? Topic modelling in a mental health Internet support group.

BACKGROUND: The majority of content in an Internet Support Group (ISG) is contributed by 1 % of the users ('super users'). Computational methods, such as topic modelling, can provide a large-scale quantitative objective description of this content. Such methods may provide a new perspective on the nature of engagement on ISGs including the role of super users and their possible effect on other users. METHODS: A topic model was computed for all posts (N = 131,004) in the ISG BlueBoard using Latent Dirichlet Allocation. A model containing 25 topics was selected on the basis of intelligibility as determined by diagnostic metrics and qualitative investigation. This model yielded 21 substantive topics for further analysis. Two chi-square tests were conducted separately for each topic to ascertain: (i) if the odds of super users' and other users' posting differed for each topic; and (ii) if for super users the odds of posting differed depending on whether the response was to a super user or to another user. RESULTS: The 21 substantive topics covered a range of issues related to mental health and peer-support. There were significantly higher odds that super users wrote content on 13 topics, with the greatest effects being for Parenting Role (OR [95%CI] = 7.97 [7.85-8.10]), Co-created Fiction (4.22 [4.17-4.27]), Mental Illness (3.13 [3.11-3.16]) and Positive Change (2.82 [2.79-2.84]). There were significantly lower odds for super users on 7 topics, with the greatest effects being for the topics Depression (OR = 0.27 [0.27-0.28]), Medication (0.36 [0.36-0.37]), Therapy (0.55 [0.54-0.55]) and Anxiety (0.55 [0.55-0.55]). However, super users were significantly more likely to write content on 5 out of these 7 topics when responding to other users than when responding to fellow super users. CONCLUSIONS: The findings suggest that super users serve the role of emotionally supportive companions with a focus on topics broadly resembling the consumer/carer model of recovery. Other users engage in topics with a greater focus on experiential knowledge, disclosure and informational support, a pattern resembling the clinical symptom-focussed approach to recovery. However, super users modify their content in response to other users in a manner consistent with being 'active help providers'.

Online interventions for problem gamblers with and without co-occurring mental health symptoms: Protocol for a randomized controlled trial.

BACKGROUND: Comorbidity between problem gambling and depression or anxiety is common. Further, the treatment needs of people with co-occurring gambling and mental health symptoms may be different from those of problem gamblers who do not have a co-occurring mental health concern. The current randomized controlled trial (RCT) will evaluate whether there is a benefit to providing access to mental health Internet interventions (G + MH intervention) in addition to an Internet intervention for problem gambling (G-only intervention) in participants with gambling problems who do or do not have co-occurring mental health symptoms. METHODS: Potential participants will be screened using an online survey to identify participants meeting criteria for problem gambling. As part of the baseline screening process, measures of current depression and anxiety will be assessed. Eligible participants agreeing (N = 280) to take part in the study will be randomized to one of two versions of an online intervention for gamblers - an intervention that just targets gambling issues (G-only) versus a website that contains interventions for depression and anxiety in addition to an intervention for gamblers (G + MH). It is predicted that problem gamblers who do not have co-occurring mental health symptoms will display no significant difference between intervention conditions at a six-month follow-up. However, for those with co-occurring mental health symptoms, it is predicted that participants receiving access to the G + MH website will display significantly reduced gambling outcomes at six-month follow-up as compared to those provided with G-only website. DISCUSSION: The trial will produce information on the best means of providing online help to gamblers with and without co-occurring mental health symptoms. TRIAL REGISTRATION: ClinicalTrials.gov NCT02800096 ; Registration date: June 14, 2016.

Community Structure of a Mental Health Internet Support Group: Modularity in User Thread Participation.

BACKGROUND: Little is known about the community structure of mental health Internet support groups, quantitatively. A greater understanding of the factors, which lead to user interaction, is needed to explain the design information of these services and future research concerning their utility. OBJECTIVE: A study was conducted to determine the characteristics of users associated with the subgroup community structure of an Internet support group for mental health issues. METHODS: A social network analysis of the Internet support group BlueBoard (blueboard.anu.edu.au) was performed to determine the modularity of the community using the Louvain method. Demographic characteristics age, gender, residential location, type of user (consumer, carer, or other), registration date, and posting frequency in subforums (depression, generalized anxiety, social anxiety, panic disorder, bipolar disorder, obsessive compulsive disorder, borderline personality disorder, eating disorders, carers, general (eg, "chit chat"), and suggestions box) of the BlueBoard users were assessed as potential predictors of the resulting subgroup structure. RESULTS: The analysis of modularity identified five main subgroups in the BlueBoard community. Registration date was found to be the largest contributor to the modularity outcome as observed by multinomial logistic regression. The addition of this variable to the final model containing all other factors improved its classification accuracy by 46.3%, that is, from 37.9% to 84.2%. Further investigation of this variable revealed that the most active and central users registered significantly earlier than the median registration time in each group. CONCLUSIONS: The five subgroups resembled five generations of BlueBoard in distinct eras that transcended discussion about different mental health issues. This finding may be due to the activity of highly engaged and central users who communicate with many other users. Future research should seek to determine the generalizability of this finding and investigate the role that highly active and central users may play in the formation of this phenomenon.

Cavity-enhanced coherent light scattering from a quantum dot.

The generation of coherent and indistinguishable single photons is a critical step for photonic quantum technologies in information processing and metrology. A promising system is the resonant optical excitation of solid-state emitters embedded in wavelength-scale three-dimensional cavities. However, the challenge here is to reject the unwanted excitation to a level below the quantum signal. We demonstrate this using coherent photon scattering from a quantum dot in a micropillar. The cavity is shown to enhance the fraction of light that is resonantly scattered toward unity, generating antibunched indistinguishable photons that are 16 times narrower than the time-bandwidth limit, even when the transition is near saturation. Finally, deterministic excitation is used to create two-photon N00N states with which we make superresolving phase measurements in a photonic circuit.

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system.

Biotherapeutics have many promising applications, such as anti-cancer treatments, immune suppression, and vaccines. However, due to their biological nature, some biotherapeutics can be challenging to rapidly express and screen for activity through traditional recombinant methods. For example, difficult-to-express proteins may be cytotoxic or form inclusion bodies during expression, increasing the time, labor, and difficulty of purification and downstream characterization. One potential pathway to simplify the expression and screening of such therapeutics is to utilize cell-free protein synthesis. Cell-free systems offer a compelling alternative to in vivo production, due to their open and malleable reaction environments. In this work, we demonstrate the use of cell-free systems for the expression and direct screening of the difficult-to-express cytotoxic protein onconase. Using cell-free systems, onconase can be rapidly expressed in soluble, active form. Furthermore, the open nature of the reaction environment allows for direct and immediate downstream characterization without the need of purification. Also, we report the ability of a "just-add-water" lyophilized cell-fee system to produce onconase. This lyophilized system remains viable after being stored above freezing for up to one year. The beneficial features of these cell-free systems make them compelling candidates for future biotherapeutic screening and production.

Effectiveness of MH-Guru, a brief online mental health program for the workplace: A randomised controlled trial.

BACKGROUND: Depression and anxiety interfere with an individual's quality of life and result in substantial economic costs to the workplace through lost productivity. The internet offers an unparalleled opportunity for the large scale, cost-effective delivery of mental health awareness and destigmatisation programs in the workplace. However, to date high quality assessments of the effectiveness of such workplace programs have been confined to health professional settings. The current study reports the effectiveness of Mental Health Guru (MH-Guru), a two-module online mental health workplace induction program. METHODS: A total of 507 employees from a large multi-departmental government workplace were randomised to a 2-week online depression and anxiety educational program (Mental Health Guru; MH-Guru), or a Wait List Control condition. Participants completed online surveys at baseline, 1 week post-intervention and at 6-month follow-up. Primary outcome measures included depression and anxiety literacy, personal stigma about depression and anxiety, and help seeking intentions for anxiety and depression at post-test. Secondary measures included help-seeking attitudes and self-reported help seeking behaviour. Satisfaction measures were also collected. RESULTS: Dropout at post intervention was 26.7% and 21.0% for the MH-Guru and Control conditions respectively. Relative to Control, MH-Guru participants showed significantly greater improvements in depression and anxiety literacy at post intervention. Between group standardized effect sizes at post-test and 6-months were 0.78 and 0.81 for depression literacy and 0.80 and 0.79 for anxiety literacy. Compared to the Control participants, the MH-Guru group also showed significantly greater reductions in depression and anxiety personal stigma. Between group effect sizes in stigma for depression were - 0.56 and - 0.47 at post-test and 6-months respectively and - 0.42 at both time points for anxiety. The MH-Guru intervention was not associated with a differentially greater increase in anxiety or depression help-seeking intentions or improvement in help seeking attitudes compared to the Control group. However, self-reported help-seeking behaviour was significantly greater in the MH-Guru group at post-test. In addition, the MH-Guru group showed greater intentions to seek help for depression from the Internet at 6-month follow-up. Satisfaction items suggested that the program was acceptable to employees. CONCLUSIONS: Brief online programs such as MH-Guru have the potential to play an important role in increasing mental health awareness and decreasing stigmatizing attitudes in the workplace, and by extension the general community.