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Int J Mol Sci ; 14(4): 7155-79, 2013 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-23538843

RESUMO

In Brazil, common bean (Phaseolus vulgaris L.) productivity is severely affected by drought stress due to low technology cultivation systems. Our purpose was to identify differentially expressed genes in roots of a genotype tolerant to water deficit (BAT 477) when submitted to an interruption of irrigation during its development. A SSH library was constructed taking as "driver" the genotype Carioca 80SH (susceptible to drought). After clustering and data mining, 1572 valid reads were obtained, resulting in 1120 ESTs (expressed sequence tags). We found sequences for transcription factors, carbohydrates metabolism, proline-rich proteins, aquaporins, chaperones and ubiquitins, all of them organized according to their biological processes. Our suppressive subtractive hybridization (SSH) library was validated through RT-qPCR experiment by assessing the expression patterns of 10 selected genes in both genotypes under stressed and control conditions. Finally, the expression patterns of 31 ESTs, putatively related to drought responses, were analyzed in a time-course experiment. Our results confirmed that such genes are more expressed in the tolerant genotype during stress; however, they are not exclusive, since different levels of these transcripts were also detected in the susceptible genotype. In addition, we observed a fluctuation in gene regulation over time for both the genotypes, which seem to adopt and adapt different strategies in order to develop tolerance against this stress.


Assuntos
Adaptação Fisiológica/genética , Secas , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Phaseolus/genética , Phaseolus/fisiologia , Raízes de Plantas/genética , Análise por Conglomerados , Etiquetas de Sequências Expressas , Genes de Plantas , Genótipo , Umidade , Raízes de Plantas/fisiologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Software , Solo , Técnicas de Hibridização Subtrativa , Fatores de Tempo , Fatores de Transcrição/metabolismo , Transcrição Gênica , Água
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