RESUMO
Subgroups of patients with oral pre-malignant lesions (OPLs) are at extremely high risk for developing invasive cancer in spite of surgical excision. The objective of this study was to evaluate the utility of specific genes and their associated centromeres as markers to stratify OPLs for their cancer risk. Samples used in this study included 35 oral dysplasia with known outcome and 20 normal oral mucosa. Of the dysplasias, 20 were from an ongoing longitudinal study showing progression. The remaining 15 cases (2 of which progressed) were chosen from the population-based, provincial BC Oral Biopsy Service (OBS). Copy number alterations at EGFR, CEP7, CCND1, and CEP11 were evaluated by fluorescent in situ hybridization (FISH). There was no significant difference in demographics between progressors and non-progressors. Specific FISH profiles at these genes and their corresponding centromeres were associated with progression. High gene gain of CCND1 was associated with an 8-fold elevated risk of progression compared with those with no gain in time-to-progression analysis. Numerical alterations of EGFR and CCND1 and their centromeres might be an effective means for identifying OPLs at risk. Future studies will expand on this analysis and set the stage for application of this approach in routine clinical practice.
Assuntos
Carcinoma de Células Escamosas/patologia , Transformação Celular Neoplásica/genética , Dosagem de Genes/genética , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/patologia , Biomarcadores Tumorais , Carcinoma de Células Escamosas/genética , Estudos de Casos e Controles , Centrômero/patologia , Instabilidade Cromossômica/genética , Ciclina D1/genética , Progressão da Doença , Receptores ErbB/genética , Feminino , Humanos , Hibridização in Situ Fluorescente , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Neoplasias Bucais/genética , Poliploidia , Lesões Pré-Cancerosas/genética , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Fatores de Risco , Sensibilidade e EspecificidadeRESUMO
BACKGROUND: Despite their histological resemblance to colorectal adenocarcinomas, there is little information about the molecular events involved in the pathogenesis of intestinal-type sinonasal adenocarcinomas (ITACs). AIMS: To evaluate the possible role of DNA mismatch repair (MMR) gene defects or disruptions of the E cadherin-beta catenin complex in ITAC by investigating the immunohistochemical expression of the MMR gene products, beta catenin, and E cadherin in a group of sporadic ITACs. METHODS: Ten sporadic cases of ITAC were stained with antibodies against MLH1, MSH2, MSH3, MSH6, beta catenin, and E cadherin. RESULTS: Nine cases showed strong nuclear expression of MLH1, whereas one case showed moderate staining. All 10 cases were strongly positive for MSH2 and MSH3. MSH6 was strong in nine cases, and moderate in one. Membranous beta catenin expression was strong in all 10 cases, and no case showed cytoplasmic or nuclear staining. E cadherin was strong in seven cases, and moderate in three cases. CONCLUSIONS: The preserved nuclear expression of MLH1, MSH2, MSH3, and MSH6 suggests that mutations or promoter methylation of MMR genes do not play a role in the pathogenesis of ITAC. The strong membranous staining for E cadherin and beta catenin and lack of abnormal cytoplasmic or nuclear expression is in keeping with the preservation of E cadherin-beta catenin complexes and beta catenin pathways.
Assuntos
Adenocarcinoma/química , Biomarcadores Tumorais/análise , Caderinas/análise , Proteínas do Citoesqueleto/análise , Neoplasias Intestinais/química , Neoplasias dos Seios Paranasais/química , Transativadores/análise , Proteínas Adaptadoras de Transdução de Sinal , Adulto , Idoso , Idoso de 80 Anos ou mais , Pareamento Incorreto de Bases , Proteínas de Transporte , Núcleo Celular/química , Citoplasma/química , Reparo do DNA , Proteínas de Ligação a DNA/análise , Feminino , Humanos , Imuno-Histoquímica/métodos , Masculino , Pessoa de Meia-Idade , Proteína 1 Homóloga a MutL , Proteína 2 Homóloga a MutS , Proteína 3 Homóloga a MutS , Proteínas de Neoplasias/análise , Proteínas Nucleares , Proteínas Proto-Oncogênicas/análise , beta CateninaRESUMO
BACKGROUND: Intestinal-type sinonasal adenocarcinoma (ITAC) is an uncommon neoplasm, which resembles adenocarcinoma of the gastrointestinal tract. ITAC occurs sporadically or in association with occupational exposure to hardwood dust and other agents. AIMS: To investigate the phenotype and possible pathogenetic mechanisms of primary sinonasal and nasopharyngeal adenocarcinomas by staining for cytokeratin 7 (CK7), CK20, CDX-2, and villin. METHODS: Twelve sporadic sinonasal and nasopharyngeal adenocarcinomas were stained with monoclonal antibodies to CK7, CK20, CDX-2, and villin. The ITACs were classified as papillary, colonic, solid, mixed, or mucinous types. RESULTS: The diagnosis of ITAC was confirmed in 10 cases: five were colonic type and five were papillary. One was a sinonasal papillary low grade adenocarcinoma, and one a papillary nasopharyngeal adenocarcinoma, and these tumours were CK7 positive, but CK20, CDX-2, and villin negative. All ITACs were positive for CK20, CDX-2, and villin, and six were CK7 positive. One ITAC had a focus of intestinal metaplasia away from the invasive carcinoma. CONCLUSIONS: Sinonasal ITACs have a distinctive phenotype, with all cases expressing CK20, CDX-2, and villin. Most ITACs also express CK7, although a proportion of tumours are CK7 negative. ITAC seems to be preceded by intestinal metaplasia of the respiratory mucosa, which is accompanied by a switch to an intestinal phenotype. Although ITACs are morphologically similar, differences in cytokeratin expression patterns suggest two distinct types. The expression pattern of CK7, CK20, CDX-2, and villin positive may be useful in separating these tumours from other non-ITAC adenocarcinomas of the sinonasal tract and nasopharynx.
Assuntos
Adenocarcinoma/química , Biomarcadores Tumorais/análise , Neoplasias Intestinais/química , Queratinas/análise , Neoplasias Nasais/química , Neoplasias dos Seios Paranasais/química , Adenocarcinoma/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Fator de Transcrição CDX2 , Proteínas de Transporte/análise , Feminino , Proteínas de Homeodomínio/análise , Humanos , Imuno-Histoquímica/métodos , Indústrias , Proteínas de Filamentos Intermediários/análise , Mucosa Intestinal/patologia , Neoplasias Intestinais/patologia , Queratina-20 , Queratina-7 , Masculino , Metaplasia , Proteínas dos Microfilamentos/análise , Pessoa de Meia-Idade , Neoplasias Nasais/patologia , Doenças Profissionais/patologia , Neoplasias dos Seios Paranasais/patologia , Transativadores , MadeiraRESUMO
BACKGROUND: Oral squamous cell carcinoma (SCC) is increasingly found in transplant recipients, although little is known of the natural history of the disease or the mechanism underlying this increase. METHODS: In this article we describe the history of development of 5 oral post-transplant SCCs (PSCCs) and compare their genetic profiles to 34 non-posttransplant SCCs (NPSCCs). RESULTS: Of the five patients with PSCCs, 3 had bone marrow transplants and two, kidney. All three PSCCs from bone marrow recipients were preceded locally by graft-vs.-host disease (GVHD). Two of the GVHD were biopsied and demonstrated dysplasia. Similar frequencies of loss of heterozygosity (LOH) occurred in PSCCs and NPSCCs at 3p, 9p, 17p and 8p, with lower frequencies in PSCCs at 4q (39% vs. 0%), 11q (53% vs. 20%) and 13q (45% vs. 20%), although the latter were not significantly different. Only 1 PSCC had a p53 mutation, compared to historical values of 40-60% for NPSCC. Interestingly, human papillomavirus (HPV) DNA was detected in 3 (60%) PSCCs, in comparison to only 4 (12%) of the 34 NPSCCs (P = 0.0346). CONCLUSIONS: Dysplasia in oral GVHD may be a strong indicator of cancer risk and should not be regarded as reactive changes to lichenoid mucosites. The low level of p53 mutation and increased HPV infection support the involvement of HPV in the development of PSCC, while the similarity in LOH patterns suggests that other aspects of carcinogenesis may be comparable in these two types of SCCs.
Assuntos
Transplante de Medula Óssea/efeitos adversos , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/virologia , Genes p53/genética , Doença Enxerto-Hospedeiro/complicações , Transplante de Rim/efeitos adversos , Neoplasias Bucais/genética , Neoplasias Bucais/virologia , Infecções por Papillomavirus/complicações , Infecções Tumorais por Vírus/complicações , Adulto , Idoso , Análise Mutacional de DNA , DNA Viral/análise , Doença Enxerto-Hospedeiro/etiologia , Doença Enxerto-Hospedeiro/patologia , Humanos , Perda de Heterozigosidade , Masculino , Pessoa de Meia-Idade , Papillomaviridae/genética , Papillomaviridae/isolamento & purificaçãoAssuntos
Adenoma/classificação , Carcinoma Papilar/classificação , Terminologia como Assunto , Neoplasias da Glândula Tireoide/classificação , Adenoma/diagnóstico , Carcinoma Papilar/diagnóstico , Carcinoma Papilar/secundário , Diagnóstico Diferencial , Humanos , Neoplasias da Glândula Tireoide/diagnósticoRESUMO
One of the best approaches to identifying genetic changes critical to oral cancer progression is to compare progressing and nonprogressing oral premalignant lesions. However, such samples are rare, and they require long-term follow-up. The current study used the large archive network and clinical database in British Columbia to study loss of heterozygosity (LOH) in cases of early oral premalignancies, comparing those with a history of progression to carcinoma in situ or invasive cancer and those without a history of progression (referred to as nonprogressing cases). Each of 116 cases was analyzed for LOH at 19 microsatellite loci on seven chromosome arms (3p, 4q, 8p, 9p, 11q, 13q, and 17p). The progressing and nonprogressing cases showed dramatically different LOH patterns of multiple allelic losses. An essential step for progression seems to involve LOH at 3p and/or 9p because virtually all progressing cases showed such loss. However, LOH at 3p and/or 9p also occurred in nonprogressing cases. Individuals with LOH at 3p and/or 9p but at no other arms exhibit only a slight increase of 3.8-fold in relative risk for developing cancer. In contrast, individuals with additional losses (on 4q, 8p, 11q, or 17p), which appeared uncommon in nonprogressing cases, showed 33-fold increases in relative cancer risk. In conclusion, analysis of LOH at 3p and 9p could serve as an initial screening for cancer risk of early premalignancies. Follow-up investigation for additional losses would be essential for predicting cancer progression.
Assuntos
Mapeamento Cromossômico , Perda de Heterozigosidade , Mucosa Bucal/patologia , Neoplasias Bucais/genética , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/genética , Lesões Pré-Cancerosas/patologia , Cromossomos Humanos Par 11 , Cromossomos Humanos Par 13 , Cromossomos Humanos Par 17 , Cromossomos Humanos Par 3 , Cromossomos Humanos Par 4 , Cromossomos Humanos Par 8 , Cromossomos Humanos Par 9 , Progressão da Doença , Feminino , Humanos , Masculino , Repetições de Microssatélites , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Retrospectivos , Fatores de RiscoAssuntos
Tonsila Faríngea/diagnóstico por imagem , Tonsila Faríngea/patologia , Bainha de Mielina/patologia , Neoplasias do Sistema Nervoso Periférico/diagnóstico por imagem , Neoplasias do Sistema Nervoso Periférico/patologia , Músculos Pterigoides/diagnóstico por imagem , Músculos Pterigoides/patologia , Sarcoma/diagnóstico por imagem , Sarcoma/patologia , Tonsila Faríngea/cirurgia , Adulto , Humanos , Imageamento por Ressonância Magnética , Masculino , Neoplasias do Sistema Nervoso Periférico/cirurgia , Músculos Pterigoides/cirurgia , Sarcoma/cirurgia , Tomografia Computadorizada por Raios XRESUMO
The distinction between a new primary oral tumor and recurrence may bear significant prognostic implications. Currently, this differentiation relies mainly on tumor location: when both lesions are at or near the same site, the new one is regarded as a recurrence; when the two are at different sites, the second lesion is regarded as a new primary. Recent investigations using molecular analysis have demonstrated that some oral squamous cell carcinomas (SCC) arising from different sites show the same clonogenical changes. In this case report, we studied the clonality of three SCC (one primary, two apparent recurrences) from the right lateral tongue of a young, non-smoking woman by using microsatellite analysis for loss of heterozygosity. The results showed that while the first two tumors were clonogenically similar, the third tumor was clonogenically different and was consistent with the development of a new primary. This result indicates that location of tumors alone is not always reliable in determining whether a new tumor is a recurrence or a new primary lesion.
Assuntos
Carcinoma de Células Escamosas/diagnóstico , Recidiva Local de Neoplasia/diagnóstico , Segunda Neoplasia Primária/diagnóstico , Neoplasias da Língua/diagnóstico , Adulto , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Diagnóstico Diferencial , Feminino , Humanos , Perda de Heterozigosidade , Recidiva Local de Neoplasia/genética , Recidiva Local de Neoplasia/patologia , Segunda Neoplasia Primária/genética , Segunda Neoplasia Primária/patologia , Neoplasias da Língua/genética , Neoplasias da Língua/patologiaRESUMO
A case of inverted Meckel's diverticulum is described. This presented as an ileal polyp in an individual with chronic unexplained iron deficiency anemia. Most prolapsed Meckel's diverticula occur acutely as intussusceptions with bowel obstruction and characteristically develop in childhood. This case therefore represents an unusual surgical problem in an older individual in which the diagnosis was clinically unexpected.
Assuntos
Neoplasias do Íleo/patologia , Pólipos Intestinais/patologia , Divertículo Ileal/patologia , Diagnóstico Diferencial , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Although it is widely accepted that clonal genetic alterations are an essential component of tumor progression, little is known of the distribution of such changes in high-risk lesions or how such clones are altered over time. We explored the feasibility of using exfoliative cells collected by scraping the mucosal surface to detect allelic loss in oral lesions of 22 patients (14 squamous cell carcinomas, 2 carcinomas in situ, and 6 dysplasias). The data show that the patterns of allelic loss observed in these samples closely represent those observed in biopsies of the same region. Furthermore, early indications are that this approach can be used to detect recurrent outgrowth of clones of altered cells in patients after therapy.
Assuntos
Carcinoma de Células Escamosas/genética , Deleção Cromossômica , Mapeamento Cromossômico , Mucosa Bucal/patologia , Neoplasias Bucais/genética , Lesões Pré-Cancerosas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Biópsia , Carcinoma in Situ/genética , Carcinoma in Situ/patologia , Carcinoma de Células Escamosas/patologia , Cromossomos Humanos , Feminino , Neoplasias Gengivais/genética , Neoplasias Gengivais/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/patologia , Fatores de Risco , Manejo de Espécimes , Neoplasias da Língua/genética , Neoplasias da Língua/patologiaAssuntos
Tumor Misto Maligno/diagnóstico , Mioepitelioma/diagnóstico , Neoplasias de Tecidos Moles/diagnóstico , Diagnóstico Diferencial , Humanos , Queratinas/análise , Tumor Misto Maligno/química , Tumor Misto Maligno/patologia , Mioepitelioma/química , Mioepitelioma/patologia , Proteínas S100/análise , Neoplasias de Tecidos Moles/química , Neoplasias de Tecidos Moles/patologiaRESUMO
Immunohistochemical analysis of the apoptosis-effector protease CPP32 (Caspase-3) in normal lymph nodes, tonsils, and nodes affected with reactive hyperplasia (n = 22) showed strong immunoreactivity in the apoptosis-prone germinal center B-lymphocytes of secondary follicles, but little or no reactivity in the surrounding long-lived mantle zone lymphocytes. Immunoblot analysis of fluorescence-activated cell sorted germinal center and mantle zone B cells supported the immunohistochemical results. In 22 of 27 (81%) follicular small cleaved cell non-Hodgkin's B-cell lymphomas, the CPP32-immunopositive germinal center lymphocytes were replaced by CPP32-negative tumor cells. In contrast, the large cell component of follicular mixed cells (FMs) and follicular large cell lymphomas (FLCLs) was strongly CPP32 immunopositive in 12 of 17 (71%) and in 8 of 14 (57%) cases, respectively, whereas the residual small-cleaved cells were poorly stained for CPP32 in all FLCLs and in 12 of 17 (71%) FMs, suggesting that an upregulation of CPP32 immunoreactivity occurred during progression. Similarly, cytosolic immunostaining for CPP32 was present in 10 of 12 (83%) diffuse large cell lymphomas (DLCLs) and 2 of 3 diffuse mixed B-cell lymphomas (DMs). Immunopositivity for CPP32 was also found in the majority of other types of non-Hodgkin's lymphomas studied. Plasmacytomas were CPP32 immunonegative in 4 of 12 (33%) cases, in contrast to normal plasma cells, which uniformly contained intense CPP32 immunoreactivity, implying downregulation of CPP32 in a subset of these malignancies. All 12 peripheral blood B-cell chronic lymphocyte leukemia specimens examined were CPP32 immunopositive, whereas 3 of 3 small lymphocytic lymphomas were CPP32 negative, suggesting that CPP32 expression may vary depending on the tissue compartment in which these neoplastic B cells reside. The results show dynamic regulation of CPP32 expression in normal and malignant lymphocytes.
Assuntos
Linfócitos B/enzimologia , Caspases , Cisteína Endopeptidases/análise , Leucemia Linfocítica Crônica de Células B/enzimologia , Linfonodos/enzimologia , Linfoma não Hodgkin/enzimologia , Proteínas de Neoplasias/análise , Animais , Caspase 3 , Progressão da Doença , Feminino , Centro Germinativo/citologia , Centro Germinativo/enzimologia , Humanos , Immunoblotting , Leucemia Linfocítica Crônica de Células B/patologia , Linfoma não Hodgkin/patologia , Tonsila Palatina/citologia , CoelhosAssuntos
Querubismo/patologia , Adulto , Querubismo/diagnóstico por imagem , Feminino , Humanos , Mandíbula/patologia , RadiografiaRESUMO
Prostate-specific antigen (PSA) is a 30 kDa glycoprotein serine protease that shows high tissue specificity for prostatic tissue, both benign and malignant. However, recent reports have shown that a variety of normal and neoplastic tissue types express PSA immunohistochemically. In addition, rare instances of the secretion of PSA by nonprostatic cancers have been reported in the literature. The authors present a case of salivary duct carcinoma associated with elevated serum levels of PSA. Both the primary tumor and metastases stained positively with anti-PSA monoclonal antibodies, but were negative with antibodies directed against prostate-specific acid phosphatase. Elevated serum PSA levels were confirmed with three different immunoassay methods. A peak serum level of 140 micrograms/L was measured and this correlates with levels of PSA associated with metastatic prostatic carcinoma. High performance liquid chromatography with a molecular sieve column characterized the serum PSA into both free protein (approximately 20%) and protein bound to alpha-1-antichymotrypsin (PSA-ACT)(approximately 80%). Molecular weights of the free PSA and PSA-ACT subfractions were 27-31 kDa and 100-110 kDa, respectively.
Assuntos
Adenocarcinoma/metabolismo , Biomarcadores Tumorais/análise , Antígeno Prostático Específico/sangue , Ductos Salivares/patologia , Neoplasias das Glândulas Salivares/metabolismo , Adenocarcinoma/química , Adenocarcinoma/patologia , Adenocarcinoma/secundário , Anticorpos Monoclonais/análise , Neoplasias Ósseas/secundário , Cromatografia Líquida de Alta Pressão , Evolução Fatal , Humanos , Técnicas Imunoenzimáticas , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Neoplasias das Glândulas Salivares/patologiaRESUMO
Quantitative image cytometry was used to compare 18 parameters relating to ploidy, nuclear area, and chromatin texture to axillary lymph node status, tumor size, and histological grade for 34 infiltrating ductal carcinomas, each of which had been graded independently by each of six surgical pathologists. Zinc formalin-fixed, paraffinembedded tumors were assessed using the Elston and Ellis modification of the Bloom and Richardson histological grading scheme. When axillary lymph node-negative tumors were compared with those involving four or more nodes, % 2 c (diploid) cells, nuclear area, and eight of 12 chromatin texture parameters showed statistically significant differences. Carcinomas < 2 cm had more % 2 c (diploid) cells and fewer % > 4 c (hypertetraploid) cells than larger neoplasms. For tumors having nuclear pleomorphism score two versus those with score three, nuclear area, four of five parameters related to ploidy level, each of five parameters related to run-length matrix features and one of four co-occurrence matrix features showed significant differences. Nearly all of these cytometric parameters also showed significant differences for histological grade and mitotic count, which was strongly correlated with nuclear pleomorphism. In examining the cytometric parameters in relation to the interobserver reproducibility of histological grade and its components, the largest number of statistically significant parameters related to the nonreproducibility of nuclear pleomorphism. The findings indicate that as the grade of infiltrating ductal carcinomas increases, there are fewer % 2 c (diploid) cells and more % > 4 c (hypertetraploid) and % > or = 5 c (polyploid) cells. In addition, the cells of high grade tumors have larger nuclear areas and more small and large dense chromatin clumps, which increase in such number that they tend to join together. When compared with the cytometric parameters, nuclear pleomorphism is the most sensitive component of grade to nonreproducibility.
Assuntos
Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/patologia , Processamento de Imagem Assistida por Computador , Neoplasias da Mama/genética , Carcinoma Ductal de Mama/genética , Feminino , Humanos , Ploidias , Prognóstico , Reprodutibilidade dos TestesRESUMO
A primary intraneural biphasic synovial sarcoma that arose within the common digital nerve of the hand is reported. The patient, a 16-year-old girl, presented with a small soft tissue mass that clinically and intraoperatively resembled a nerve sheath tumor. The diagnosis of synovial sarcoma was established on the basis of a typical light microscopic appearance and immunohistochemical staining profile. Intraneural biphasic synovial sarcoma resembles "glandular" peripheral nerve sheath tumor, because both are composed of a mixture of spindled mesenchymal cells with admixed glandular epithelial elements. The features allowing differentiation of these two unusual tumors of peripheral nerve are discussed.
Assuntos
Neoplasias Epiteliais e Glandulares/imunologia , Neoplasias Epiteliais e Glandulares/patologia , Neoplasias do Sistema Nervoso Periférico/patologia , Sarcoma Sinovial/patologia , Adolescente , Feminino , Humanos , Imuno-Histoquímica , Neoplasias do Sistema Nervoso Periférico/imunologia , Sarcoma Sinovial/imunologiaRESUMO
Olfactory neuroblastoma (ONB) is a malignant tumor of the nasal mucosa whose histogenesis is unclear. A relationship to neuroblastoma (NB), a pediatric tumor of the sympathetic nervous system, is based on morphologic similarities and the expression of similar neural antigens. However, the clinical presentation of ONB differs from that of NB, and MYCN amplification characteristic of NB is not observed. We have therefore examined the relationship of this malignancy to other classes of neural tumors. In previous studies, two ONB cell lines demonstrated cytogenetic features and patterns of protooncogene expression suggestive of a relationship to the Ewing sarcoma family of childhood peripheral primitive neuroectodermal tumors (pPNETs). The pPNETs show t(11;22)(q24;q12) or t(21;22)(q22;q12) chromosomal translocations fusing the EWS gene from 22q12 with either the FL11 gene on 11q24 or the ERG gene on 21q22. We therefore analyzed ONBs for the presence of pPNET-associated gene fusions. Both cell lines showed rearrangement of the EWS gene, and fluorescence in situ hybridization (FISH) of each case demonstrated fusion of EWS and FL11 genomic sequences. Moreover, both lines expressed EWS/FL11 fusion transcripts with in-frame junctions between exon 7 of EWS and exon 6 of FL11 as described for pPNETs. We identified similar gene fusions in four of six primary ONB cases. None of the cases expressed tyrosine hydroxylase, a catecholamine biosynthetic enzyme widely expressed in NB. Our studies indicate that ONB is not a NB but is a member of the pPNET family.
Assuntos
Neoplasias Ósseas/genética , Neuroblastoma/genética , Tumores Neuroectodérmicos Primitivos/genética , Neoplasias Nasais/genética , Sarcoma de Ewing/genética , Adulto , Neoplasias Ósseas/classificação , Linhagem Celular , Criança , Mapeamento Cromossômico , Cromossomos Humanos Par 11 , Cromossomos Humanos Par 21 , Cromossomos Humanos Par 22 , Primers do DNA , Feminino , Expressão Gênica , Rearranjo Gênico , Ribonucleoproteínas Nucleares Heterogêneas , Humanos , Hibridização in Situ Fluorescente , Masculino , Pessoa de Meia-Idade , Mucosa Nasal , Proteínas de Neoplasias/genética , Neuroblastoma/classificação , Tumores Neuroectodérmicos Primitivos/classificação , Neoplasias Nasais/classificação , Reação em Cadeia da Polimerase , Proteína EWS de Ligação a RNA , Proteínas Recombinantes de Fusão/biossíntese , Ribonucleoproteínas/biossíntese , Ribonucleoproteínas/genética , Sarcoma de Ewing/classificação , Translocação Genética , Células Tumorais CultivadasRESUMO
PURPOSE: To their knowledge, the authors report the first recognized case of ductal adenocarcinoma of the lacrimal gland (histologic equivalent of salivary duct carcinoma). Primary adenocarcinoma of the lacrimal gland is rare and has been described generically. In contrast, primary adenocarcinomas of the major and minor salivary glands are much more common and have been classified into histopathologic subtypes that have different clinical characteristics and outcomes. METHODS: A 68-year-old man presented with a 6-month history of a painless mass in the right upper outer eyelid. The authors describe the clinical, radiologic, and histopathologic features of this case and review the lacrimal gland literature. RESULTS: A modified en bloc orbitectomy was performed, and postoperative radiotherapy was administered. The patient was alive and well without evidence of tumor recurrence 10 months after surgery. CONCLUSION: The World Health Organization classification of salivary adenocarcinomas (1991) provides a framework for further insight into the presentation and biologic behavior of the less common lacrimal carcinomas.
Assuntos
Carcinoma Ductal de Mama/patologia , Doenças do Aparelho Lacrimal/patologia , Idoso , Carcinoma Ductal de Mama/química , Carcinoma Ductal de Mama/terapia , Humanos , Técnicas Imunoenzimáticas , Queratinas/análise , Doenças do Aparelho Lacrimal/terapia , Masculino , Órbita/diagnóstico por imagem , Radioterapia Adjuvante , Ductos Salivares/patologia , Neoplasias das Glândulas Salivares/patologia , Tomografia Computadorizada por Raios XRESUMO
Solitary oncocytic cyst of the larynx is a recognized clinical and pathologic entity. Multiple oncocytic cysts have only rarely been described. A 67-year-old female presented with rapidly progressive airway obstruction requiring emergency tracheotomy. The obstruction was caused by multiple cystic lesions throughout the supraglottic region. Microscopic examination of laryngeal biopsies showed cysts within the lamina propria, lined by oncocytic epithelium. The differential diagnosis and pathogenesis of oncocytic cysts is discussed with a review of other laryngeal cystic lesions.