Characterization and evasion of backscattered light in the squeezed-light enhanced gravitational wave interferometer GEO 600.

Squeezed light is injected into the dark port of gravitational wave interferometers, in order to reduce the quantum noise. A fraction of the interferometer output light can reach the OPO due to sub-optimal isolation of the squeezing injection path. This backscattered light interacts with squeezed light generation process, introducing additional measurement noise. We present a theoretical description of the noise coupling mechanism and we prove the model with experimental results. We propose a control scheme to achieve a de-amplification of the backscattered light inside the OPO with a consequent reduction of the noise caused by it. The scheme was implemented at the GEO 600 detector and has proven to be crucial in maintaining a good level of quantum noise reduction of the interferometer for high parametric gain of the OPO. In particular, the mitigation of the backscattered light noise helped in reaching 6 dB of quantum noise reduction [Phys. Rev. Lett.126, 041102 (2021)10.1103/PhysRevLett.126.041102]. We show that the impact of backscattered-light-induced noise on the squeezing performance is phenomenologically equivalent to increased phase noise of the squeezing angle control. The results discussed in this paper provide a way for a more accurate estimation of the residual phase noise of the squeezed light field. Finally, the knowledge of the backscattered light noise coupling mechanism is a useful tool to inform the design of the squeezing injection path in terms of path stability and optical isolation.

Direct limits for scalar field dark matter from a gravitational-wave detector.

The nature of dark matter remains unknown to date, although several candidate particles are being considered in a dynamically changing research landscape1. Scalar field dark matter is a prominent option that is being explored with precision instruments, such as atomic clocks and optical cavities2-8. Here we describe a direct search for scalar field dark matter using a gravitational-wave detector, which operates beyond the quantum shot-noise limit. We set new upper limits on the coupling constants of scalar field dark matter as a function of its mass, by excluding the presence of signals that would be produced through the direct coupling of this dark matter to the beam splitter of the GEO600 interferometer. These constraints improve on bounds from previous direct searches by more than six orders of magnitude and are, in some cases, more stringent than limits obtained in tests of the equivalence principle by up to four orders of magnitude. Our work demonstrates that scalar field dark matter can be investigated or constrained with direct searches using gravitational-wave detectors and highlights the potential of quantum-enhanced interferometry for dark matter detection.

First Demonstration of 6 dB Quantum Noise Reduction in a Kilometer Scale Gravitational Wave Observatory.

Photon shot noise, arising from the quantum-mechanical nature of the light, currently limits the sensitivity of all the gravitational wave observatories at frequencies above one kilohertz. We report a successful application of squeezed vacuum states of light at the GEO 600 observatory and demonstrate for the first time a reduction of quantum noise up to 6.03±0.02 dB in a kilometer scale interferometer. This is equivalent at high frequencies to increasing the laser power circulating in the interferometer by a factor of 4. Achieving this milestone, a key goal for the upgrades of the advanced detectors required a better understanding of the noise sources and losses and implementation of robust control schemes to mitigate their contributions. In particular, we address the optical losses from beam propagation, phase noise from the squeezing ellipse, and backscattered light from the squeezed light source. The expertise gained from this work carried out at GEO 600 provides insight toward the implementation of 10 dB of squeezing envisioned for third-generation gravitational wave detectors.

Essential role of antigen-presenting cell-derived BAFF for antibody responses.

Antigen-presenting cells (APC) are directly involved in survival, growth and differentiation of naive B cells and in immunoglobulin class switch recombination. Less is known about the contribution of APC to memory B cell responses. We employed an in vitro model to investigate the secondary humoral response against foot-and-mouth disease virus, with cells from a natural host of the virus - the pig. This response is T cell-dependent. Under conditions of limited T cell help, defined as a low T-to-B cell ratio or by the replacement of T cells with interleukin-2 only, the antibody response was dependent on APC. These included monocytes and monocyte-derived DC, but not plasmacytoid DC. APC mediated their help through soluble factors, particularly soluble B cell-activating factor belonging to the TNF family (BAFF). Our results suggest that the 'ménage à trois' concept, saying that both APC and T cells have a direct effect in B cell activation, is also valid for secondary B cell responses, and imply an important role for BAFF under conditions that might be physiologically relevant in secondary lymphoid organs.

Porcine B-cell activating factor promotes anti-FMDV antibodies in vitro but not in vivo after DNA vaccination of pigs.

'B-cell activating factor belonging to the TNF family' (BAFF) represents a cytokine produced by antigen presenting cells promoting B-cell maturation, activation and immunoglobulin class switching. In the present study, we demonstrate expression of BAFF on cultured monocyte-derived dendritic cells, which is further enhanced by interferon-alpha or interferon-gamma treatment. From these cells, porcine BAFF was cloned and the recombinant protein was expressed in mammalian cells with and without a FLAG tag at the carboxyl terminus. Only the protein without the FLAG tag was bioactive in vitro, and promoted B-cell survival and the differentiation of foot-and-mouth disease virus (FMDV)-specific memory B cells into antibody producing cells. Based on this result it was tested whether BAFF can enhance FMDV antibody responses in the context of a DNA vaccination. To this end, pigs were immunised with the anti-FMDV DNA vaccine plasmid pcDNA3.1/P1-2A3C3D and a pCI plasmid expressing porcine BAFF. Using a needle-free transdermal application method, also referred to as 'jet injection', pigs were vaccinated three times and their humoral response quantified by ELISA and a virus neutralisation test. After the third vaccination, three out of six animals vaccinated with the pcDNA3.1/P1-2A3C3D alone but none of the animals that also received the BAFF expressing plasmid had seroconverted. These data suggest that BAFF is not appropriate as a genetic adjuvant when applied as a simple co-injection with the antigen-encoding plasmid.

Plasmacytoid dendritic cell activation by foot-and-mouth disease virus requires immune complexes.

Natural IFN-producing cells (NIPC), also called plasmacytoid dendritic cells, represent an essential component of the innate immune defense against infection. Despite this, not much is known about the pathways involved in their activation by non-enveloped viruses. The present study demonstrates that the non-enveloped foot-and-mouth disease virus (FMDV) cannot stimulate IFN-alpha responses in NIPC, unless complexed with FMDV-specific immunoglobulins. Stimulation of NIPC with such immune complexes employs FcgammaRII ligation, leading to strong secretion of IFN-alpha. In contrast to the stimulation of NIPC by many enveloped viruses, FMDV induction of IFN-alpha production requires live virus. It is necessary for the virus to initiate its replicative cycle. Moreover, it is an abortive replication, as witnessed by the decrease of dsRNA levels and viral titers with time post infection. Sensitivity of the NIPC stimulation to wortmannin and chloroquin, but not leupeptin, indicates an essential role for the pre-lysosomal stage endosomal compartment. In conclusion, the present study demonstrates that immune complexes provide the means for a non-interferogenic virus to induce IFN-alpha responses by NIPC. This indicates an important link between NIPC and antibodies in immune responses against non-enveloped viruses such as FMDV.