RESUMO
Umbilical discharge is a symptom of a varied pathology. We analysed the files of 22 patients with umbilical discharge operated on in our hospital over a period of 23 years. In 13 patients the umbilical discharge was accompanied by an acute umbilical inflammation. In 10 patients the symptoms were caused by embryonic anomalies and in 12 patients by an acquired pathology. We propose a surgical technique that can be applied regardless of the pathology, and that does not involve a pre-operative assessment of the causes.
Assuntos
Fístula Cutânea/diagnóstico , Exsudatos e Transudatos , Umbigo , Adenocarcinoma/diagnóstico , Adenocarcinoma/secundário , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Endometriose/diagnóstico , Feminino , Humanos , Inflamação/diagnóstico , Masculino , Pessoa de Meia-Idade , Artérias Umbilicais/anormalidades , Umbigo/anormalidades , Umbigo/embriologia , Cisto do Úraco/diagnóstico , Ducto Vitelino/anormalidadesRESUMO
A quantitative evaluation of IgA, IgD, IgG and IgM plasmocytes in sequential bone marrow aspirates of patients with acute leukemia using the peroxidase-antiperoxidase method was undertaken. Plasmocytosis resembled that of normal controls or was slightly subnormal on admission. When remission was obtained, bone marrow plasmocytosis was similar to normal controls, irrespective of the type of acute leukemia and the cytostatic treatment. Occurrence of infection strongly augmented the number of plasmocytes, with an initial increase in IgM and later in IgG plasmocytes. This suggests that the immune response is preserved in patients with acute leukemia.
Assuntos
Medula Óssea/patologia , Leucemia/patologia , Plasmócitos/patologia , Doença Aguda , Adulto , Idoso , Medula Óssea/imunologia , Feminino , Humanos , Imunoglobulina A/análise , Imunoglobulina D/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Leucemia/imunologia , Masculino , Pessoa de Meia-Idade , Plasmócitos/imunologiaRESUMO
Plasma cell cytoplasmic immunoglobulin was stained using the peroxidase-antiperoxidase technic in Bouin-fixed, paraffin-embedded human tissues from different origins. Bone marrow (BM), tonsils, and appendices were examined. IgA-, IgD-, and IgM-secreting plasmocytes were easily studied using highly diluted rabbit antihuman antisera in all tissues, including BM. IgG plasmocytes showed good stainability in tonsils and appendices, but variable results were obtained in BM. Bone marrow IgG plasmocytes from persons without infection required a tenfold higher concentration of rabbit antihuman IgG than plasmocytes derived from patients with infection. Stainability of BM plasmocytes from patients with infection was equal to BM plasmocytes from myeloma patients. Because the same rabbit antihuman IgG concentration could be applied for staining plasmocytes derived from tonsils and appendices, it is most probable that the difference in staining ability is due to a difference in activity of the plasmocytes, i.e., a different IgG concentration in the plasmocytes.
Assuntos
Apêndice/patologia , Medula Óssea/patologia , Técnicas Imunoenzimáticas , Imunoglobulina G , Tonsila Palatina/patologia , Plasmócitos/imunologia , Coloração e Rotulagem/métodos , Doença Aguda , Citoplasma/imunologia , Humanos , Infecções/imunologia , Leucemia/imunologiaRESUMO
Detectable ABH blood group antigens (BGAg) on tumor cells from transitional carcinoma of the urinary bladder, used for diagnosis and prognosis may be evidenced with the existing immunoperoxidase methods. In this study, a modification of the immunoperoxidase method was shown to enable the detection of BGAg in deparaffinized tissue section of normal and malignant human urothelium. To detect antigens A and B a peroxidase-conjugated rabbit anti-human IgM was used and antigen H was visualized by biotinylated lectin.
