RESUMO
Metazoan histone mRNAs are unique in that they lack a 3'-polyadenylated tail, but instead end in a conserved stem loop that is bound by SLBP (stem-loop binding protein). SLBP is required for efficient histone mRNA synthesis and translation. Removal of SLBP by RNA interference causes an increase in the number of cells in S-phase and this effect can be reversed by expressing an exogenous SLBP resistant to the small interfering RNA. Cells with decreased SLBP levels progress slowly through S-phase when released from a double-thymidine block. Thus SLBP is required for efficient DNA replication probably because a decreased ability to assemble chromatin results in a decrease in the rate of DNA replication.
Assuntos
Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Interferência de RNA , Fase S , Fatores de Poliadenilação e Clivagem de mRNA/genética , Fatores de Poliadenilação e Clivagem de mRNA/metabolismo , Animais , Linhagem Celular , HumanosRESUMO
The ability of fast thire-and fourth-generation scanning devices to supply images of sufficient quality to delineate the kidney, its vascular supply, and surrounding fascial envelope has provided a modality which permits preoperative staging of tumors of the kidney with an accuracy of about 90% in limited series of 20 specimen cases. The unreliability of bolus injection to evaluate tumor extension to the renal vein accounted for two errors.
Assuntos
Adenocarcinoma/diagnóstico por imagem , Neoplasias Renais/diagnóstico por imagem , Estadiamento de Neoplasias/métodos , Tomografia Computadorizada por Raios X , Adenocarcinoma/patologia , Humanos , Neoplasias Renais/patologiaRESUMO
Potentially lethal consequences can quickly occur once the mediastinum is subjected to the ravages of an anaerobic infection. Mediastinitis from odontogenic or deep cervical infections is extremely rare in the era of antibiotic drugs. We have recently encountered five such cases, with a rapid spread of the inflammatory process into the mediastinum resulting in a number of local and systemic complications. All were caused by anaerobic bacteria. Awareness of such complications and early roentgenographic diagnosis lead to prompt surgical drainage, proper antibiotic therapy, and survival after a stormy clinical course. The anatomic pathways between the various fascial planes of the neck and mediastinum will be described.
Assuntos
Infecções Bacterianas/etiologia , Infecção Focal Dentária , Mediastinite/etiologia , Pescoço , Adolescente , Adulto , Idoso , Criança , Feminino , Infecção Focal Dentária/etiologia , Humanos , MasculinoRESUMO
Radionuclide venography in two patients with suspected deep venous disease revealed double channel drainage with cross-pelvic collateral circulation. Contrast inferior venacavograms revealed that one patient had persistent right and left supracardinal veins (double inferior vena cava) and the other had iliac vein occlusion with ascending lumbar collateral circulation. The similarity of radionuclide venographic findings in these two patients emphasizes the importance of contrast venacavography in patients with this appearance on radionuclide venography.
Assuntos
Veia Ilíaca/diagnóstico por imagem , Flebografia , Veia Cava Inferior/anormalidades , Adulto , Circulação Colateral , Feminino , Humanos , Masculino , Cintilografia , Doenças Vasculares/diagnóstico por imagem , Veia Cava Inferior/diagnóstico por imagemRESUMO
We have classified esophageal trauma into three major categories: (1) Iatrogenic trauma including endoscopic perforation, trauma due to dilatation, surgical trauma, tube trauma, radiation trauma, and drug trauma; (2) Self-induced trauma including foreign bodies, corrosive or drug ingestion, and postemetic trauma; (3) Direct trauma including penetrating trauma and blunt trauma. Discussion and illustrative cases are presented.
Assuntos
Esôfago/lesões , Cateteres de Demora/efeitos adversos , Cáusticos/efeitos adversos , Dilatação , Perfuração Esofágica/diagnóstico por imagem , Perfuração Esofágica/etiologia , Esofagoscopia/efeitos adversos , Esôfago/anatomia & histologia , Esôfago/diagnóstico por imagem , Corpos Estranhos , Humanos , Complicações Pós-Operatórias , Lesões por Radiação , Radiografia , Vômito/complicações , Ferimentos não Penetrantes , Ferimentos PenetrantesRESUMO
Entrapment of chest tubes under one or more sternal sutures is an uncommon complication of median sternotomy. Five cases occurred among 759 median sternotomies performed during a 2 year period. A radiographic sign of chest tube entrapment is presented. Early recognition may prevent a second surgical procedure.
Assuntos
Intubação , Complicações Pós-Operatórias , Esterno/cirurgia , Humanos , Radiografia Torácica , SuturasRESUMO
Radionuclide angiography is a rapid, safe, and reliable method of imaging aneurysms of the abdominal aorta in patients who are too ill to undergo contrast angiography or when time is a critical factor.
Assuntos
Aneurisma Aórtico/diagnóstico , Cintilografia , Idoso , Aorta Abdominal , Humanos , Masculino , Ruptura Espontânea/diagnóstico , TecnécioRESUMO
The postoperative chest radiographs of 173 patients who underwent median sternternal line. A total of 52 (30%) showed a thin lucent line one or more days after the operation. Clinical dehiscence developed in 4 patients, of whom 2 had a midsternal stripe and 2 did not. The authors feel that the presence of a sternal stripe is of little value in the radiographic diagnosis of sternal dehiscence.
Assuntos
Esterno/diagnóstico por imagem , Deiscência da Ferida Operatória/diagnóstico por imagem , Humanos , Radiografia , Esterno/cirurgiaRESUMO
Granulomatous inflammation of the peritoneal surfaces resulting from exposure to cornstarch granules from surgical gloves produces a syndrome of abdominal pair, adynamic ileus, fever, peritonitis, variable white blood cell count, and inflammatory ascites. Symptoms develop three to four weeks after a routine abdominal surgical procedure. Recognition of this entity by nonsurgical means is necessary to avoid reoperation. Paracentesis with examination of fluid by polarized light offers the best method of non-surgical diagnosis. Treatment is with steroids or indomethacin or conservative measures. To prevent the disease, gloves must be washed effectively before operation.