Mitochondria-Assisted Photooxidation to Track Singlet Oxygen at Homeostatic Membrane Microviscosity.

Using intracellular-controlled photochemistry to track dynamic organelle processes is gaining attention due to its broad applications. However, most of the employed molecular probes usually require toxic photosensitizers and complex bioanalytical protocols. Here, the synthesis and performance of two new subcellular probes (MitoT1 and MitoT2) are described. The probes undergo photooxidation in the damaged tissue of zebrafish, a model system for tissue regeneration studies. Using high-resolution confocal microscopy and fluorescence spectroscopy, we combine the mentioned photoinduced interconversion at the homeostatic membrane viscosity to track singlet oxygen activity selectively. The continuous and real-time biosensing method reported here provides a new approach for simultaneously detecting endogenous singlet oxygen and viscosity status.

Organotin(iv) differential fluorescent probe for controlled subcellular localization and nuclear microviscosity monitoring.

A dual-emissive fluorescent probe enabling dynamic changes in nuclear local microviscosity monitoring was developed. The new sensing scenario involves probe subcellular localization redistribution, allowing a quantitative analysis of the local microviscosity related to nuclear damage in the presence of agents perturbing the nuclear morphology. With the aid of an organotin(iv) in situ formed complex we propose a different scenario of bioanalytical applications through confocal microscopy.