RESUMO
The importance of evaluating receptors for estrogen and progestin in human breast cancer has been pointed out by many authors. In the absence of a reference standard, receptor assays must be controlled by intra and interlaboratory quality control programs. Much interlaboratory variability exists due to non-uniform analytical protocols, non-uniform ligands, intrinsic errors and also errors in computation methods. The goals of our Italian Quality Control Program on Multicenter Trials are to standardize the analytical procedures and computation methods. Twenty Italian laboratories participated in the Quality Control Program. Each specimen was assayed for steroid receptor content according to the standardized dextran-coated-charcoal method. Data were subjected to computerized analyses by 5 different methods of calculation (Scatchard plot, direct plot, Lineweaver-Burk method, Brunauer-Emmet-Teller analysis, single-point approach). The results were than evaluated to identify intra- and inter-assay variation coefficients and to define other statistical parameters. The authors suggest different calculation methods depending on the specific experimental and/or physiopathological conditions.
Assuntos
Neoplasias da Mama/análise , Receptores de Estrogênio/análise , Computadores , Feminino , Humanos , Itália , Matemática , Métodos , Controle de Qualidade , Receptores de Progesterona/análise , Estatística como AssuntoRESUMO
This study concerns the relationship between spontaneous revertants and number of bacterial cells plated in the Ames test. These two parameters do not appear to bear a direct relationship: marked variations in cell input are attended by negligible variations in spontaneous revertants. However, a relationship, described by a quadratic equation that approximates a hyperbole, exists between the spontaneous revertants and the number of total cellular divisions permitted by the histidine concentration in the medium. Furthermore a direct linear relationship has been observed between cell input and diameter of the plate background microcolonies. The problem of the toxic effect in the Ames test performed with drugs or substances having high bactericidal activity is discussed.
Assuntos
Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Testes de Mutagenicidade/métodos , Divisão Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Doxorrubicina/toxicidade , Histidina/análise , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/crescimento & desenvolvimentoRESUMO
This article presents results of the first Italian quality control program for determining the estradiol receptor on lypophilized guinea pig and calf uteri. Despite considerable variability in quantitative terms, the results concur in ability to define samples as positive or negative for receptor content. One of the parameters that most strongly influences accuracy of determination of receptor concentration is protein assay. The evaluation of several lyophilized preparations at scalar concentrations permitted identification, by linear regression, for each laboratory of the systematic and non-systematic variables. More comparable results will be forthcoming when a standardized methodology program has been fully adopted.