Determining the heritable component of dairy cattle foot lesions.

Lameness and hoof health affect dairy cows as an animal welfare issue, in decreased milk production, and in premature culling. Selection schemes for dairy cattle focus on sire contribution to milk production, with little consideration of the cow's physical structure or disease probability. On 3 commercial California dairies, 6 phenotypic binary hoof traits that contribute to lameness were recorded: white line disease, sole ulcer, other claw horn lesions, foot rot (interdigital phlegmon), foot warts (digital dermatitis), and other lesions. Monthly lactation records were collected from December 2006 to April 2009 with weekly observations of hoof lesions for lame and dry cows. In addition to hoof lesion information, data on cows (n=5,043) included parentage, birth date, freshening date, lactation number, and date of lameness diagnosis. The prevalence of hoof lesions ranged from a low of 2.2% (foot rot) to a high of 17.1% (foot warts). The farm environment increased the odds ratio depending upon the lesion. Lameness was more common in early lactation and as lactation number increased. Using a threshold model, heritabilities and repeatabilities were estimated for each binary trait. The heritability for risk varied by lesion, with the higher estimates being 0.40 (95% confidence interval: 0.20-0.67) for digital dermatitis and 0.30 (95% confidence interval: 0.08-0.63) for sole ulcer. Including terms to account for cow productivity on either a 305-d mature-equivalent basis or a per-lactation basis had minimal effect on the heritability estimates, suggesting that selection for hoof health is not correlated with response to selection for greater milk production and that improvement could be made for both traits. The genetic component lends support for further genetic studies to identify loci contributing to some of the lesion phenotypes such as foot warts or sole ulcers, 2 of the top 3 causes of lameness in dairy cattle.

Restless behavior increases over time, but not with compressibility of the flooring surface, during forced standing at the feed bunk.

Interest in the use of rubber flooring in freestall barns has increased, but little is known about which design features of these surfaces are important for cattle. In 2 experiments, we evaluated how the type and compressibility of the flooring surface in front of the feed bunk influenced the behavioral response to 4 h of forced standing after morning milking. Two flooring types were compared: rubber and concrete. Rubber was tested at 3 levels of compressibility: 2, 4, and 35 times as compressible as concrete. Four hours of forced standing was evaluated because it mimicked conditions that can occur on dairies, particularly when waiting for artificial insemination or veterinary treatment. The effects of cow weight and hoof surface area, gait score, and hoof health on the response to treatment were evaluated. Restless behavior, as measured by number of steps, almost doubled over the 4h of forced standing, regardless of flooring material. Cows lay down, on average, within 5 min after access to the lying area was provided. These results indicate that the 4 h of forced standing was uncomfortable. No differences in restless behavior were observed in association with the type or compressibility of the flooring surface in front of the feed bunk. Cow size, hoof health, or gait score did not consistently explain the response to the flooring treatments or stepping rate, although these populations of animals were generally healthy. It is unclear if comfort did not differ between the flooring options tested during 4 h of forced standing or if alterative methodology, such as measuring more subtle shifts in weight, is required to assess design features of rubber flooring.

Effect of rubber flooring on claw health in lactating dairy cows housed in free-stall barns.

Multiparous dairy cows between 10 to 30 d in milk (DIM) were enrolled in a clinical trial to evaluate the effects of rubber flooring on the development of claw lesions, locomotion scores, clinical lameness, and rates of hoof growth and wear. Two groups of cows were housed in identical free-stall facilities, except that 1 pen (rubber, n = 84) had rubber alley mats covering the entire concrete floor of the pen, whereas cows in the second pen were exposed to concrete flooring (concrete, n = 82) without rubber alley mats. All cows were evaluated 3 times between 10 and 30, 74 and 94, and 110 and 130 DIM for 1) the presence of claw lesions on their rear feet, 2) the occurrence of clinical lameness based on a locomotion score, and 3) rates of claw growth and wear as observed on the dorsal wall of the right lateral claw. No differences between flooring groups at the time of enrollment were detected for lactation number, mean DIM at first examination, body condition score, and proportion of cows with claw lesions at the first examination. Odds of developing claw lesions between examinations were not different for cows exposed to the rubber surface compared with those exposed to concrete. Cows on concrete, however, had greater odds of developing or exacerbating existing heel erosion than cows on rubber flooring. Regardless of the flooring surface, the lateral claw was more likely to develop lesions than the medial claw. Odds of becoming lame by the third examination and the proportion of cows requiring therapeutic hoof trimming because of lameness were greater for concrete-exposed cows than those on rubber. Cows on rubber flooring had decreased claw growth and wear between the first and last examination compared with cows on concrete. Regardless of flooring surface, second-lactation cows had greater wear rates than those in third or greater parities. Results of our study suggest that a soft flooring surface, such as interlocking rubber, is beneficial for hoof health.

A comparison of three animal welfare assessment programs on California dairies.

Due to increased awareness of animal welfare issues by consumers, 3 voluntary programs were developed to assess animal welfare on commercial dairies in the United States. These programs include: 1) Humane Farm Animal Care (HFAC), with minimum standards and a third-party audit system; 2) Dairy Quality Assurance Center (DQAC); and 3) University of California-Davis (UCD) program with self-assessments. The objective of this article was to provide an overview of the similarities and differences in content and scope of these programs. Furthermore, to ascertain the practical application of these programs on commercial dairies, a study was designed to compare the rank order of compliance scores of each program on 10 commercial California dairies using a survey and personal interviews with dairy owners or managers. The survey combined 316 statements from the DQAC checklist, the UCD assessment, and the HFAC standards. The mean (+/-SD) percentage compliance for the DQAC, HFAC, and UCD programs was 83 +/- 8, 94 +/- 3, and 85 +/- 6%, respectively. A ranking order from 1 to 10 was assigned to each dairy using the compliance score for each assessment program. Comparisons of ranking order between programs were not significant, indicating that different indices were being measured by each program. All programs, however, identified the same 2 lowest-ranking dairies, which indicated that the programs were able to identify facilities with the weakest welfare practices. The survey results show that selection of the available assessment programs for animal welfare on the dairy is important in determining the outcomes.

Efficacy of a nonantimicrobial cream administered topically for treatment of digital dermatitis in dairy cattle.

OBJECTIVE: To compare efficacy of a topically administered nonantimicrobial cream with that of lincomycin for treatment of digital dermatitis in dairy cattle. DESIGN: Randomized clinical trial. ANIMALS: 98 cows from a commercial Holstein dairy herd. PROCEDURE: Cows with active lesions of digital dermatitis identified on a single observation day were randomly assigned to receive a nonantimicrobial cream, lincomycin paste, or no treatment. Cows were examined approximately every 4 weeks for 130 days after treatment for lesion maturity score, score for signs of pain, lesion size, and lesion activity. RESULTS: 29 days after a single treatment, both treated groups had significantly reduced scores for signs of pain, lesion activity, lesion size, and the decision to retreat, compared with findings in the untreated group. Efficacy of the 2 treatments was not significantly different for decreasing pain score or lesion activity or for increasing lesion maturity score, but lincomycin was significantly more efficacious in decreasing lesion size and avoiding retreatment. By use of multivariate logistic regression, lactation number was a significant treatment effect modifier on the outcome of a healed lesion after treatment. Cows with > or = 3 lactations were more likely to have a healed lesion at 29 days, compared with first- and second-lactation cows. CONCLUSIONS AND CLINICAL RELEVANCE: Because antimicrobial treatments for digital dermatitis in cows require a veterinarian's prescription, the nonantimicrobial cream could serve as a viable but less consistently effective alternative to antimicrobials and could be applied by veterinarians, hoof trimmers, and others.

Diseases of the digital soft tissues.

This article reviews diseases of the digital soft tissues, including digital dermatitis, interdigital dermatitis, interdigital phlegmon, and interdigital hyperplasia. The clinical signs and diagnosis, epidemiology and pathogenesis, etiology, and treatment and prevention of each disease are discussed.

Pain location and psychological characteristics of patients with chronic pain.

The authors assessed psychological characteristics of 140 medical outpatients with chronic non-malignant pain referred for psychiatric consultation. Subjects completed the Toronto Alexithymia Scale, Somatosensory Amplification Scale (SSAS), and Counter-dependency Scale (CDS). The only psychological measure able to differentiate the chronic pain group from the control subjects was the CDS. However, SSAS scores were significantly higher in subjects having pain involving the head, chest, abdomen, or pelvis than in subjects having pain only in their backs or extremities. The latter subgroup had significantly higher CDS scores. The findings suggest that there are discrete subgroups within the chronic pain population defined by pain location and specific psychological characteristics.

Switching antipsychotic therapies.

BACKGROUND: Atypical antipsychotics are superior to conventional antipsychotics in improving positive and negative psychotic symptoms. Atypical antipsychotics do not exacerbate mood symptoms, and may improve mood symptoms and cognitive functioning; additionally, they have better adverse effect profiles than conventional antipsychotics. OBJECTIVE: To review the benefits of switching patients with schizophrenia or schizoaffective disorder from a conventional to an atypical antipsychotic, or from one atypical antipsychotic to another. In spite of the higher acquisition cost of atypical antipsychotics, overall treatment costs may decrease due to lower relapse and hospitalization rates. DATA SOURCES: A MEDLINE search (January 1977-January 1999) was conducted for articles written in English about efficacy, adverse effects, compliance, and pharmacoeconomics for atypical and conventional antipsychotics. STUDY SELECTION: Large, multicenter, double-blind, controlled studies were used for efficacy, safety, tolerability, and pharmaco-economic data. Where appropriate, recent review articles were also used. RESULTS: Atypical antipsychotics are superior to conventional antipsychotics in the treatment of schizophrenia. Atypical and conventional antipsychotics have different adverse effect profiles, costs, and compliance rates. CONCLUSIONS: Some patients may benefit by switching from a conventional to an atypical antipsychotic, from an atypical to a conventional antipsychotic, or from one atypical antipsychotic to another. Methods of switching antipsychotic therapies include tapering and cross-over strategies.

Measuring counterdependency in patients with chronic pain.

OBJECTIVE: Some reports have characterized patients with chronic pain as counterdependent, that is, having emotional suppression, idealization of relationships, strong work ethic, a caregiver role-identity, and self-reliance. However, research has been hampered because formal measures of these traits have been lacking. In this article, we describe a five-item self-report questionnaire, the Counterdependency Scale (CDS), designed to elicit each of these traits on a Likert scale. METHODS: The CDS was administered to 150 consecutive patients evaluated in an outpatient psychiatry consultation program. RESULTS: CDS scores were normally distributed and had significant interitem correlations and test-retest reliability (r = 0.68). As expected, subjects with chronic pain (N = 100) had higher mean CDS scores than those without chronic pain (t = 5.6, p = .000). CDS scores were independent of demographic variables and measures of anxiety, depression, alexithymia, and somatic amplification. CONCLUSIONS: These results suggest that counterdependency can be described by a distinct and measurable cluster of traits associated with chronic pain.

Phenelzine-induced sexual dysfunction treated with sildenafil.

This article describes the presence, in a 56-year-old male, of sexual dysfunction that was associated with phenelzine (Nardil) and was treated successfully with sildenafil (Viagra). The sexual dysfunction was assessed using the Arizona Sexual Experiences Scale for males (ASEX-Male).

Effects of antimicrobial treatment at the end of lactation on milk yield, somatic cell count, and incidence of clinical mastitis during the subsequent lactation in a dairy herd with a low prevalence of contagious mastitis.

OBJECTIVE: To determine whether treating cows with antimicrobials at the end of lactation would lower the incidence of clinical mastitis, improve milk production, and decrease somatic cell count (SCC) in the subsequent lactation. DESIGN: Randomized blind field trial. ANIMALS: 233 Holstein cows from a single herd. All cows were in lactation 2 or greater. PROCEDURE: Cows were randomly assigned to treatment groups. Treated cows were given procaine penicillin G and novobiocin by intramammary infusion. Control cows were not treated. Farm personnel recorded cases of clinical mastitis. Milk yield and SCC were recorded during the subsequent lactation. RESULTS: Treatment did not significantly reduce the incidence of clinical mastitis when data for all cows were grouped or when data were stratified by lactation groups (lactation 2 vs lactation > or = 3) or by last SCC (< or = 500,000 cells/ml vs > 500,000 cells/ml). Somatic cell counts (first, mean of first 5, maximum of first 5) for treated and control cows were similar, and proportions of treated and control cows with SCC > 500,000 cells/ml at least once were not significantly different. Treated cows produced 179 kg (394 lb) more milk during the first 17 weeks of lactation than did control cows. CLINICAL IMPLICATIONS: Treating cows with antimicrobials at the end of lactation increased 17-week milk production during the subsequent lactation and, at current milk prices, was financially preferable to not treating them.

Humoral response of dairy cattle to spirochetes isolated from papillomatous digital dermatitis lesions.

OBJECTIVE: To determine whether a humoral response against spirochetes isolated from papillomatous digital dermatitis (PDD) lesions is elicited in dairy cattle affected with PDD. SAMPLE POPULATION: 41 cattle with PDD from 8 dairies (study population) and 30 cattle from 2 dairies free of PDD (control population). Additionally evaluated were 32 cattle from a dairy with a past history of PDD but no current disease, and 52 cattle from a dairy with high prevalence of PDD, 25 with and 27 without detectable lesions. PROCEDURE: ELISA were used to evaluate the humoral response of all cattle to representative isolates from 2 groups of spirochetes of unknown species isolated from PDD lesions. Specificity of the response was evaluated, using immune sera prepared against each of the spirochetes, and by adsorption studies of immune and field sera. The potential for confounding by an antibody response to other spirochetes associated with diseases of cattle was assessed. RESULTS: The antibody response (specific) to both PDD spirochete groups of cows with PDD was significantly increased, compared with that of cows from PDD-free dairies. There was no association between antibody response to PDD-associated spirochetes and antibody response to other spirochetal diseases of cattle. None of the cattle from the dairy with previous history of PDD but without current disease were classified as test positive by either PDD ELISA. There was a significant (P < 0.01) difference in classification results for both PDD ELISA for cattle with PDD from the dairy with a high herd prevalence of PDD, compared with cattle without detectable disease from the same dairy. CONCLUSIONS AND CLINICAL RELEVANCE: The humoral response in cattle with PDD lesions was significantly different from that in cattle without detectable lesions, thus providing additional information regarding the potential role of spirochetes isolated from PDD lesions in the etiopathogenesis of PDD.

Pregnancy status and feedlot performance of beef heifers actively immunized against gonadotropin-releasing hormone.

The contraceptive effect of active immunization against GnRH was evaluated in beef heifers. Crossbred heifers were randomized by breed and weight (initial weight = 227.3 +/- 1.2 kg) and assigned to one of three treatment groups. Animals in Group IB (n = 48) were actively immunized against GnRH; heifers in Groups NB (n = 48) and NN (n = 49) did not receive the anti-GnRH vaccine. Sixteen weeks after primary immunization, bulls of proven fertility were introduced into pens containing Groups IB and NB. Bulls were maintained with heifers for 2 mo. Heifers in group NN were not intentionally exposed to fertile males. At the end of the breeding period, heifers received Synovex H implants and entered a commercial feedlot. Heifers were slaughtered after 116 d of feedlot confinement. Anti-GnRH titer was evident in all heifers (48 of 48) immunized against GnRH. Gravid uteri were present at slaughter in 40 of 48 (83.3%) NB heifers. In contrast, only four (8.3%) IB and two (4.1%) NN heifers carried gravid uteri at slaughter. Although ADG did not differ between groups during feedlot confinement, ADG during the breeding period was higher (P < .05) in IB heifers than in NN control animals. Dressing percentage and longissimus muscle area were decreased (P < .05) and marbling and quality grade were increased (P < .05) in NB heifers compared with NN control heifers. Carcass traits of IB heifers were intermediate between those of the NB and NN groups. Taken together, these data indicate that active immunization against GnRH reduces the fertility of terminal heifers. These observations suggest that immunoneutralization of GnRH may be an effective management tool that will reduce the incidence of unintended pregnancy in heifers destined for feedlots.

Sensitivity and specificity analysis for somatic cell count (SCC) used to predict bacteriologically positive subclinical mastitis at calving in a dairy herd with low SCC.

OBJECTIVE: Validate, by sensitivity and specificity analyses, use of somatic cell count (SCC) to predict bacteriologically positive subclinical mastitis in a California dairy herd with low SCC. DESIGN: Study of monthly dairy herd improvement SCC obtained from the immediate preceding lactation and individual cow composite milk sample microbiologic isolates collected at calving. ANIMALS: 515 California dairy cows with SCC and culture data. PROCEDURE: Somatic cell count sensitivity and specificity analyses with combinations of SCC parameter and at various thresholds were done, using the bacterial isolates as the standard. RESULTS: Combination of SCC threshold and SCC parameters could not be developed that had sufficient sensitivity and specificity to be a useful predictor of cows that would calve with subclinical mastitis. CLINICAL IMPLICATIONS: Under the conditions at this particular dairy, SCC could not be used as a basis of prediction of cows that would calve with bacteriologically positive subclinical mastitis or require selective nonlactating-cow antibiotic treatment.

Characterisation of proteins from two-dimensional electrophoresis gels by matrix-assisted laser desorption mass spectrometry and amino acid compositional analysis.

Amino acid compositional analysis and peptide mass fingerprinting by matrix assisted laser desorption mass spectrometry have been used to characterise proteins obtained from two-dimensional electrophoresis (2-DE) separations of human cardiac proteins. A group of twelve protein spots was selected for analysis. The identities of eight of the proteins had been determined by conventional protein characterisation methods, two were unknown proteins and two had putative identities from protein database spot comparison. Amino acid analysis and peptide mass fingerprinting gave corresponding identities for seven of the twelve proteins, which also agreed with our initial identifications. Three proteins which had been identified previously were not confirmed in this study and putative identities were obtained for the two unknown proteins. The advantages, problems and use of amino acid analysis and peptide mass fingerprinting for the analysis of proteins from 2-DE are discussed. The data highlight the need to use orthogonal techniques for the unequivocal identification of proteins from 2-DE gels.

Psychological factors affecting reported pain in juvenile rheumatoid arthritis.

Examined the extent to which psychological variables are correlated with pain reported by children with juvenile rheumatoid arthritis (JRA). In a hierarchical multiple regression analysis with pain as the dependent variable, four psychological measures of child and family functioning resulted in a significant increase in R2 = .31, p < .0001, after the effects of disease characteristics were considered. Greater emotional distress in the child, greater emotional distress of the mother, and greater family harmony were related to higher reported pain. Findings suggest that more attention should be given to nonpharmacological aspects of pain and pain management in children with JRA.

Clinical syndrome and diagnosis of hyperkalaemic periodic paralysis in quarter horses.

Of the 16 horses studied, 14 belonged to a family previously shown to be susceptible to hyperkalaemic periodic paralysis (HPP), and 8 were shown to have HPP. Diagnosis of HPP by electromyographic detection of myotonic discharges or by oral administration of KCl to induce clinical signs and hyperkalaemia had similar reliability and gave the same result in 80% of cases. KCl had to be administered at doses up to 0.2 g/kg bodyweight to produce signs in some horses. KCl challenge testing was more time consuming than EMG and resulted in one fatality. Overall, electromyography and potassium challenge testing together gave the most accurate diagnosis. Horses were more likely to manifest signs of HPP if they were immature. There was no sex predisposition. The most frequent sign of HPP was muscle fasciculation. Other signs were sweating, muscle spasm, and weakness. Respiratory rate increased greatly during some attacks. Attacks of muscle fasciculation without hyperkalaemia were observed in 2 HPP-affected horses. Affected horses had a greater frequency of spontaneous clinical abnormalities, due mainly to trailering problems, traumatic abrasions and episodes of continuous muscle fasciculation. Hyperkalaemia was confirmed in only one of these instances.

Conceptions of illness by children with juvenile rheumatoid arthritis: a cognitive developmental approach.

Investigated the conceptions of illness and accuracy of understanding about their disease for children with juvenile rheumatoid arthritis (JRA). 54 children between the ages of 6 and 17 were interviewed individually about various aspects of JRA, with results suggesting that accuracy and illness conceptions could be reliably measured. As predicted, children's understanding about their disease followed a developmental progression, with older children demonstrating a more sophisticated understanding of JRA than younger children (significant differences between age groups on 3 of the 5 questions). Multiple regression analysis indicated that conceptual level (p < .001) was a better predictor of the child's accuracy of knowledge than was age (ns). Despite the developmental progression, there were a significant number of children functioning below the level expected for their age. In fact, the majority (75%) of children exhibited an understanding of JRA at the concrete operational level of cognitive development. The within-subject variability and striking misconceptions argue for ongoing evaluation of each child's understanding as a way to improve educational efforts.

Evaluation of a psychological treatment package for treating pain in juvenile rheumatoid arthritis.

We examined the utility of psychological treatment procedures for children with high levels of pain associated with juvenile rheumatoid arthritis (JRA). By the use of a multiple baseline across subjects design, four children were assigned to an immediate treatment group, and four children to a delayed treatment group. The six-session treatment included relaxation training, electromyogram, and thermal biofeedback for the child; mothers were trained in the use of behavioral techniques for managing physical therapy and school attendance. Visual inspection of the data indicates small changes on children's self-reported pain diary scores for mean pain and ratings of high (greater than 5 on a 10-point visual analogue scale) pain periods, with 50% to 62% showing at least a 25% reduction in pain immediately after treatment, and 62% to 88% showing a 25% reduction by 6-month follow-up. Maternal reports of changes paralleled those of the children. Comparisons of Mann-Whitney U-tests conducted pre- and posttreatment indicated no differences for children's ratings of mean pain or +5 pain ratings between the immediate and delayed treatment groups; greater improvement for the immediate treatment group was noted on maternal reports of both mean pain (p < 0.05) and +5 pain (p < 0.5) ratings. The reduction of pain reports from pretreatment to follow-up was significant for children's mean pain (p = 0.02), +5 pain ratings (p = 0.02), and mother's reports of mean pain (p = 0.03) and +5 pain periods (p = 0.01). Maternal reports of the number of pain-related behaviors that the child exhibited also declined (p < 0.05). No reduction in physical therapist's ratings of pain during evaluation were noted. No increases in maternal reports of child's psychological adjustment problems were reported following treatment. Results provide modest support for the use of psychological interventions with patients with JRA.