RESUMO
The seven member, human MORF4 related gene (MRG) family was recently identified based on the ability of Mortality factor on chromosome 4 (MORF4) to induce replicative senescence in immortal cell lines assigned to complementation group B (Bertram et al., 1999. Mol. Cell Biol. 19, 1479-1485). Initial computer based similarity searches identified human retinoblastoma binding protein 1 (RBP-1), Drosophila melanogaster male specific lethal-3 (Msl-3), S. pombe altered polarity-13 (Alp13) and S. cerevisiae Eaf3p, a component of the yeast NuA4 HAT complex (Galarneau et al., 2000. Mol. Cell 5, 927-937), as having similarity to the human MRG protein family. This suggested that the MRG family might be found in multiple species, and analysis of other homologs would provide functional and evolutionary insights into this gene family. Here, we report that MRG family members are present in twenty-three species based on molecular assays and sequence similarity searches. The new family members were divided into two groups based on similarity to the predominant human MRG family members, MRG15 and MRGX. The family members similar to MRG15 define a new, highly conserved subsection of the chromo domain superfamily. Additionally, conservation in the C-terminal two thirds of all the MRG family members and the Drosophila and human MSL-3 proteins defines a new protein domain, the MRG domain. These results indicate a highly conserved role for the MRG family in transcriptional regulation via chromatin remodeling by histone acetylation.
Assuntos
Sequência Conservada/genética , Fatores de Transcrição/genética , Motivos de Aminoácidos/genética , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Southern Blotting , Bovinos , Galinhas , Cromatina/metabolismo , DNA/genética , Bases de Dados Factuais , Cães , Haplorrinos , Humanos , Camundongos , Dados de Sequência Molecular , Filogenia , Coelhos , Ratos , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/metabolismoRESUMO
Approximately 50% of immortal human keratinocyte lines show loss of heterozygosity of chromosome region 4q33-q34, and the reintroduction of chromosome 4 into one such line, BICR 6, causes proliferation arrest and features of replicative senescence. Recently, a candidate gene, mortality factor 4 (MORF4), was identified in this region and sequenced in 21 immortal keratinocyte lines. There were no mutations or deletions, and two of the seven lines that showed loss of heterozygosity at 4q33-q34 were heterozygous for MORF4 itself. Furthermore, the transfer of a chromosomal segment containing the entire MORF4 gene did not mimic the senescence effect of chromosome 4 in BICR 6. These results suggest that the inactivation of MORF4 is not required for human keratinocyte immortality.
Assuntos
Senescência Celular/genética , Queratinócitos/citologia , Fatores de Transcrição/fisiologia , Linhagem Celular Transformada , Cromossomos Humanos Par 4/genética , Fibroblastos/citologia , Genótipo , Humanos , Perda de Heterozigosidade , Reação em Cadeia da Polimerase , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologia , Fatores de Transcrição/genética , Células Tumorais CultivadasRESUMO
We have isolated and characterized a novel cDNA, C1q-Related Factor (CRF), that is predicted to encode a 258 amino acid polypeptide with a hydrophobic signal sequence, a collagenous region, and a globular domain at the carboxy terminus that shares homology to the C1q signature domain. Human CRF transcript is expressed at highest levels in the brain, particularly in the brainstem. In situ hybridization to mouse brain sections demonstrated that CRF transcripts are most abundant in areas of the nervous system involved in motor function, such as the Purkinje cells of the cerebellum, the accessory olivary nucleus, the pons and the red nucleus. The mouse CRF homolog is highly similar to the human gene at both the nucleotide and protein level, suggesting an important conserved role for this protein.
Assuntos
Química Encefálica/fisiologia , Ativação do Complemento , Complemento C1q/isolamento & purificação , Atividade Motora/fisiologia , Sequência de Aminoácidos , Animais , Clonagem Molecular , Humanos , Camundongos , Dados de Sequência Molecular , Biossíntese de Proteínas , RNA/biossíntese , Homologia de Sequência de AminoácidosRESUMO
Based on the dominance of cellular senescence over immortality, immortal human cell lines have been assigned to four complementation groups for indefinite division. Human chromosomes carrying senescence genes have been identified, including chromosome 4. We report the cloning and identification of a gene, mortality factor 4 (MORF 4), which induces a senescent-like phenotype in immortal cell lines assigned to complementation group B with concomitant changes in two markers for senescence. MORF 4 is a member of a novel family of genes with transcription factor-like motifs. We present here the sequences of the seven family members, their chromosomal locations, and a partial characterization of the three members that are expressed. Elucidation of the mechanism of action of these genes should enhance our understanding of growth regulation and cellular aging.
Assuntos
Senescência Celular/genética , Fatores de Transcrição/genética , Sequência de Aminoácidos , Sequência de Bases , Divisão Celular/genética , Linhagem Celular , Núcleo Celular/metabolismo , Expressão Gênica , Teste de Complementação Genética , Humanos , Dados de Sequência Molecular , Família Multigênica , Sondas de Oligonucleotídeos/genética , Fenótipo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/metabolismoRESUMO
The accessory gland of male insects produces components of the seminal fluid that alter the behavior, physiology and life span of the mated female, and contribute to her efficient storage and utilization of sperm. As a step towards understanding how this occurs, we have isolated genes encoding 12 previously unreported accessory gland-specific mRNAs from the fruit fly Drosophila melanogaster. We report here the restriction maps of the new genes, the chromosome positions--which are all autosomal--of the 11 non-repetitive genes, their expression patterns, and the sequences of the accessory gland proteins (Acps) encoded by nine of the genes. Eight of the proteins predicted from these sequences begin with putative secretion signals. Following their signal sequences, three of the predicted molecules are peptides and the other five are larger polypeptides with characteristics of cleavable prohormones. The ninth molecule, which has an N-terminal hydrophobic region but no consensus signal peptide cleavage site, is predicted to be a 716 amino acid glycoprotein. Of the nine proteins, two have intriguing similarities to sequences in protein databases. Acp76A is a 388 amino acid pro-protein which contains a signature sequence for the serpin class of protease inhibitors. The 115 amino acid Acp62F has a 28 amino acid region of high sequence similarity to a neurotoxin of the Brazilian armed spider Phoneutria nigriventer. Models are discussed in which Acp76A plays a role in the observed regulation of Acp proteolysis and/or in the coagulation of seminal fluid to form a mating plug, and in which Acp62F contributes to the reported toxicity of Drosophila seminal fluid.
Assuntos
Drosophila melanogaster/genética , Genes de Insetos , Proteínas de Insetos/genética , Sêmen/fisiologia , Animais , Sequência de Bases , Feminino , Expressão Gênica , Proteínas de Insetos/biossíntese , Masculino , Dados de Sequência Molecular , Biossíntese Peptídica/genética , RNA Mensageiro/genética , Reprodução/genética , Reprodução/fisiologia , Mapeamento por Restrição , Análise de Sequência de DNARESUMO
In many insect species, sperm transferred in a single mating are stored by the female in specialized organs and are gradually used to fertilize eggs. Thus, insects must have mechanisms to ensure that substantial numbers of sperm reach and become stored in the storage organs. We report here that a glycoprotein, Acp36DE, made by the accessory glands of Drosophila melanogaster males, shows localization in the mated female suggesting a role in sperm storage. In the mated female, Acp36DE associates with the wall of the oviduct, just anterior to the openings of the sperm storage organs. Acp36DE also associates with the leading edge of the sperm mass. It does not enter the hemolymph. Complete localization of Acp36DE in the mated female requires sperm and the presence of eggs in the ovaries. We hypothesize that Acp36DE, or a complex containing it, forms a barrier that "corrals" sperm near the openings to the sperm storage organs. Concentration of sperm here could facilitate their efficient storage. Acp36DE remains in the genital tract for several hours after mating, concurrent with the time that sperm are being stored. Facilitation of sperm storage by Acp36DE may also explain the previously observed effect of this protein on sperm competition.
Assuntos
Drosophila melanogaster/metabolismo , Glicoproteínas/metabolismo , Espermatozoides/metabolismo , Animais , Feminino , Genitália Feminina/metabolismo , Masculino , Oviductos/metabolismo , Comportamento Sexual AnimalRESUMO
The accessory gland of the male Drosophila melanogaster plays a vital role in reproduction. This secretory organ synthesizes products that are transferred to the female and are necessary to elicit the proper physiological and behavioral responses in the female. The accessory gland is composed of two morphologically distinct secretory cell types, the main cells and the secondary cells. Previous studies identified some genes expressed in main cells or in all accessory gland cells. In this paper we use P-element mediated enhancer traps to examine gene expression in the accessory gland. We show that, in addition to genes expressed in main cells only or in all accessory gland secretory cells, there are genes expressed specifically in secondary cells. Each cell type is uniform in the expression of its genes. Our results demonstrate that the two cell types are not only morphologically distinct but also biochemically distinct. We also show that the two cell types differ in their regulation of gene expression in response to mating activity.