RESUMO
The murine homologue of the human motility-stimulating protein autotaxin (ATX) was identified as a BMP2 upregulated gene by subtractive cloning from mesenchymal progenitors C3H10T1/2 (Bächner, D., Ahrens, M., Betat, N., Schröder, D., Hoffmann. A., Lauber, J., Steinert, P., Flohe, L., Gross, G., 1998. Bmp-2 downstream targets in mesenchymal development identified by subtractive cloning from recombinant mesenchymal progenitors (C3H10T1/2). Dev. Dyn. 213, 398-411). ATX mRNA transcription is induced during BMP2 mediated osteo-/chondrogenic differentiation in vitro several orders of magnitude. To delineate a potential role for ATX in osteo-/chondrogenic development, its expression pattern during murine embryogenesis was examined in comparison with Col1a1 and Col2a1, a marker either of osteoblast, odontoblast and tendon or of chondrocyte development, respectively. Localization of murine ATX was first observed in the floor plate of the neural tube at day 9.5 of mouse embryonic development. Later, enhanced ATX expression levels were observed in proliferating subepithelial mesenchyme, during osteo-/chondrogenic and tooth development, in choroid plexus epithelium, in late kidney development, and in smooth muscles of the ductus deferens and the bladder.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Glucose-6-Fosfato Isomerase/genética , Glicoproteínas/genética , Complexos Multienzimáticos , Animais , Animais Recém-Nascidos , Osso e Ossos/embriologia , Osso e Ossos/metabolismo , Região Branquial/embriologia , Região Branquial/metabolismo , Colágeno/genética , Colágeno/metabolismo , Embrião de Mamíferos/metabolismo , Glucose-6-Fosfato Isomerase/metabolismo , Glicoproteínas/metabolismo , Humanos , Rim/embriologia , Rim/metabolismo , Botões de Extremidades/metabolismo , Mesoderma/metabolismo , Camundongos , Camundongos Endogâmicos , Fosfodiesterase I , Diester Fosfórico Hidrolases , Pirofosfatases , Dente/embriologia , Dente/metabolismoRESUMO
Apolipoprotein E (ApoE) was identified as upregulated by Bmp-2 (bone morphogenetic protein-2) in the murine mesenchymal progenitor cell line C3H10T1/2 by a subtractive cloning strategy. Expression of recombinant Bmps in mesenchymal C3H10T1/2 progenitors results in the differentiation into the osteogenic, the chondrogenic, and the adipogenic lineage. In addition, ApoE is also expressed in primary osteoblasts isolated from murine calvariae late in the in vitro osteoblast developmental sequence. To infer possible roles of ApoE in organogenesis and tissue differentiation, ApoE expression during mouse embryonic development was analyzed in murine midgestation and late embryonic development by in situ hybridization. ApoE is highly expressed at many sites of organ development (liver, brain, heart, eye, lung), probably in a subset of neural crest cells and ectodermal derivatives suggestive for important functions of ApoE during embryonic differentiation and organ development.
Assuntos
Apolipoproteínas E/genética , Proteínas Morfogenéticas Ósseas/fisiologia , Embrião de Mamíferos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Mesoderma/citologia , Células-Tronco/metabolismo , Animais , Apolipoproteínas E/metabolismo , Proteínas Morfogenéticas Ósseas/genética , Diferenciação Celular , Linhagem Celular , Linhagem da Célula , Células Cultivadas , Clonagem Molecular , Embrião de Mamíferos/citologia , Desenvolvimento Embrionário e Fetal , Hibridização In Situ , Mesoderma/metabolismo , Camundongos , Camundongos Endogâmicos , Crista Neural/citologia , Crista Neural/embriologia , Crista Neural/metabolismo , Osteoblastos/citologia , Osteoblastos/metabolismo , Proteínas Recombinantes/metabolismo , Transfecção , Regulação para CimaRESUMO
ABmp-dependent in vitro model was used to identify cDNAs during the manifestation of mesenchymal lineages. This model involves the recombinant expression of Bmps (Bmp-2, Bmp-4-7) in murine mesenchymal C3H10T1/2 progenitors, which leads to the differentiation into three lineages: the osteogenic, the chondrogenic and the adipogenic lineage, albeit in varying efficiencies. By subtractive cloning, 21 Bmp-2-regulated cDNAs from C3H10T1/2 mesenchymal progenitors were identified; 20 were related to known sequences and 1 was not. During mouse embryonic development, many of these cDNAs are expressed in chondrogenic, osteogenic, and in adipogenic tissues. Novel findings include a G0/G1 switch gene (G0S2), which was demonstrated to be predominantly expressed in adipose tissue during late murine embryonic development. Furthermore, the membrane-standing glycoprotein autotaxin (ATX) is expressed, at precartilage condensations, joint regions, and during tooth development. An as yet undescribed cDNA, 29A, which encodes a putative secreted factor, is expressed in developing osteo-/chondrogenic tissues of vertebrae, ribs, tooth, and the limb bud. C3H10T1/2-progenitors, therefore, may serve as a legitimate model for the investigation of the Bmp-mediated events during mesenchymal differentiation.
Assuntos
Proteínas Morfogenéticas Ósseas/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Fator de Crescimento Transformador beta , Regulação para Cima , Sequência de Aminoácidos , Animais , Sequência de Bases , Proteína Morfogenética Óssea 2 , Proteínas Morfogenéticas Ósseas/genética , Proteínas Estimuladoras de Ligação a CCAAT , Linhagem da Célula , Condrócitos , DNA Complementar , Proteínas de Ligação a DNA/metabolismo , Mesoderma , Camundongos , Camundongos Endogâmicos C3H , Dados de Sequência Molecular , Proteínas Nucleares/metabolismo , Osteoblastos , Células-TroncoRESUMO
The complete cDNA for murine Beta-trace protein was isolated by RT-PCR using degenerate primers designed according to amino acid sequences derived from tryptic peptides. It encodes a protein of 165 amino acids (calculated molecular weight 18,472 Da) with a predicted 24-amino-acid leader peptide. In situ analyses during mouse embryonic development and in adult animals revealed a specific temporal expression pattern of Beta-trace. Beta-Trace mRNA was initially detected at 14.5 days postconception in mesenchymal cells destined to become leptomeninges and in the developing testis. Later in development, a lower level of expression was additionally observed in choroid plexus epithelium, in strictly confined regions of the eye (pigment and ciliary epithelium), in the ear (cochlear duct), and within single cells in the brain. Expression was also found in epithelia of the epididymis and the testis Leydig cells of postpubertal animals. The highly specific expression at blood-tissue barriers such as the blood-cerebrospinal fluid, blood-retina blood-aqueous humor, and blood-testis barriers indicates a potential role for this lipocalin in transport and/or in maturation and maintenance of these barriers.