Effect of physical cues of altered extract media from biodegradable magnesium implants on human gingival fibroblasts.

Volume stable barrier membranes made of magnesium are very promising in Guided Bone Regeneration (GBR) to treat periodontal bone defects in dentistry due to their excellent biocompatibility and biodegradability. During the degradation process the cells are exposed to the alteration of various parameters, so called physical cues, involving surface alterations due to the formed corrosion layer and medium alterations arising from the dissolved corrosion products. Cell migration of human gingival fibroblasts (HGF), as a crucial parameter for optimal healing process in GBR, has been investigated on magnesium membranes and revealed that medium alterations by dissolved corrosion products have a higher impact on cell migration than surface alterations. However, the effect of each altered medium parameter on cell migration has not been adequately studied, but their roles are crucial to explain the slower migration rate on magnesium surfaces compared to titanium and tissue culture plastic surfaces. Our study investigates the single effect of Mg2+, Ca2+, H2 and increased osmolality as well as the effect of magnesium extracts, which contain a dynamic mixture of previous parameters on cell migration, proliferation and viability of HGF. We showed that at 75 mM Mg2+ concentration and at 0 mM Ca2+, respectively, the cell migration rate is greatly reduced. In complex magnesium extract media, we found that a temporarily increased ratio of Mg2+ to Ca2+ conditioned a slow HGF migration rate. Based on these findings and the characterization of supernatants from HGF migration assays on Mg membranes, we propose, that the slower migration rate of HGF can be explained by the altered ratio of Mg2+ to Ca2+, caused by increasing concentrations of Mg2+ and decreasing concentrations of Ca2+ in the vicinity of the corroding Mg implant, combined with a constantly increased molecular hydrogen concentration in the supernatant. These results are cell type specific and should be checked carefully, if necessary, for Mg implant performance. STATEMENT OF SIGNIFICANCE: The study is providing a systematic approach to explain the main effects of extract medium parameters (physical cues) such as magnesium or calcium ion concentration, osmolality and dissolved molecular hydrogen and CO2 in cell culture media modified by co-incubating with corroding magnesium implants on the migration rate of human gingival fibroblasts (HGF). This study uncovers for the first time the combinatory effect of slightly increased molecular hydrogen and the change in Mg2+/Ca2+ ratio on HGF cell migration.

Implementing a new curriculum for computer-assisted restorations in prosthetic dentistry.

INTRODUCTION: Computer-aided design/computer-aided manufacturing (CAD/CAM) of fixed prosthetic restorations has gained popularity in the last decade. However, this field of dentistry has not been integrated in the dental curriculum at most universities. MATERIAL AND METHODS: According to the method of Kern, a curriculum was designed and established on a voluntary basis in the prosthetic education of a German dental school. The success of the implementation was measured by evaluation carried out by the participants on a visual analogue scale. Furthermore, the clinical performance of the fabricated restorations was evaluated. RESULTS: Ninety-four percent of all students participated in the CAD/CAM curriculum indicating considerable interest. Nearly half of all students used the acquired knowledge to design crowns for their patients. All restorations fabricated by participants of the new CAD/CAM programme showed good clinical performance. DISCUSSION: By phasing-in the CAD/CAM training programme, independent CAD/CAM-based fabrication of all-ceramic crowns increased student's self-confidence in tooth preparation. A tendency was found that students using CAD/CAM technology prepared more teeth than their fellow students who did not use CAD/CAM technology. Further studies are required to investigate the influence of independent CAD/CAM-based single-crown fabrication on the quality of the preparation.

Histo-anatomic 3D printing of dental structures.

The creation of dental restorations with natural appearance and biomechanics represents a major challenge for the restorative team. The manufacturing-process of high-aesthetic restorations from tooth-coloured restorative materials is currently dominated by manual manufacturing procedures and the outcome is highly dependent on the knowledge and skills of the performing dental technician. On the other hand, due to the simplicity of the manufacturing process, CAD/CAM restorations from different material classes gain more and more acceptance in the daily routine. Multi-layered restorations show significant aesthetic advantages versus monolithic ones, but are difficult to fabricate using digital technologies. The key element for the successful automated digital fabrication of aesthetic anterior restorations seems to be the form of the individual dentine core as defined by dentine enamel junction (DEJ) covered by a more transparent layer of material imitating the enamel layer to create the outer enamel surface (OES). This article describes the possibilities and technologies available for so-called '4D-printing'. It introduces the digital manufacturing process of multilayered anterior teeth using 3D multipart printing, taking the example of manufacturing replicas of extracted intact natural teeth.

Wearing complete dental prostheses - Effects on perioral morphology.

BACKGROUND: To adequately perform rehabilitation of edentulous patients by a complete removable dental prosthesis (CRDP) is from basic interest to dentists to understand the morphologic changes caused by re-establishment of a physiologic jaw relationship. Anthropometric analyses of standardized frontal view and profile photographs may help elucidate such changes. MATERIAL AND METHODS: Photographs of 31 edentulous patients were compared in relaxed lip closure and after insertion of a CRDP in stable occlusion. 2232 anthropometric distances were raised. Eighteen anthropometric indices reflecting the perioral morphology and its integration in the vertical facial harmony were investigated. RESULTS: The intercanthal - mouth width index (p>.001), medial - lateral cutaneous upper lip height index (p=.007), lower vermilion contour index (p=.022), vermilion - total upper lip height index (p=.018), cutaneous - total upper lip height index (p=.023), upper lip - nose height index (p=.001), nose - upper face height index (p=.002), chin - mandible height index (p=.013), upper lip - mandible height index (p=.045), nose - lower face height index (p=.018), and nose - face height index (p=.029) showed significant pre- to post-treatment changes. CONCLUSIONS: The investigated anthropometric indices presented reproducible results related to an increase in occlusal vertical dimension. Their application may be helpful in assessment, planning, and explanation of morphologic effects of CRDPs on the perioral and overall facial morphology, which may helps to improve the aesthetic outcome.

Influence of plasma pretreatment on shear bond strength of self-adhesive resin cements to polyetheretherketone.

OBJECTIVES: The aim of this study is to evaluate the adhesion between PEEK and two self-adhesive resin cements after plasma treatment. METHODS: Eight hundred sixty-four polyetheretherketone (PEEK) disks were cut and polished to silicon carbide (SIC) P4000. One half of the specimens were randomly selected and pretreated with plasma, whereas the remaining 432 specimens remained untreated. Subsequently, specimens were randomly allocated to four groups (n = 108/group): Visio.link (Bredent), Signum PEEK Bond (Heraeus Kulzer), Ambarino P60 (Creamed), and a control group without additional treatment. Half of the specimens of each group (n = 54) were then cemented with either RelyX Unicem Automix 2 (3 M ESPE) or with Clearfil SA (Kuraray). All specimens were stored in water for 24 h (37 °C). Afterwards, specimens were divided into three groups (n = 18) for different aging levels: (1) no aging (baseline measurement), (2) thermal aging for 5,000 cycles (5/55 °C), and (3) thermal aging for 10,000 cycles (5/55 °C). Thereafter, shear bond strengths (SBS) were measured, and failure types (adhesive, mixed, and cohesive) were assessed. Data were analyzed using descriptive statistics, four- and one-way ANOVA followed by a post hoc Scheffé test (p < 0.05). RESULTS: No adhesion could be established without adhesive pretreatment, irrespectively, whether plasma was applied or not. Also, no bond strength was measured when Ambarino P60 was applied. In contrast, adhesive pretreatment resulted in SBS ranging between 8 and 15 MPa. No significant differences were found between the resin cements used. In general, no cohesive failures were observed. Groups without plasma treatment combined with Visio.link or Signum PEEK Bond showed predominantly mixed failure types. Control groups, plasma treated, or treated using Ambarino P60 groups fractured predominantly adhesively. CONCLUSION: The use of methyl methacrylate (MMA)-based adhesives allows bonding between PEEK and self-adhesive resin cements. Plasma treatment has no impact on bond to resin cements. CLINICAL SIGNIFICANCE: PEEK reconstructions can be cemented using self-adhesive resin cements combined with pretreatment with MMA-based adhesives.

Are implants more reliable than severely compromised endodontically treated teeth as abutments for zirconia-based FPDs? : In vitro results of long-term preclinical load simulation.

OBJECTIVES: The aim was to study the impact of the defect size of endodontically treated incisors compared to dental implants as abutments on the survival of zirconia two-unit anterior cantilever-fixed partial dentures (2U-FPDs) during 10-year simulation. MATERIALS AND METHODS: Human maxillary central incisors were endodontically treated and divided into three groups (n = 24): I, access cavities rebuilt with composite core; II, teeth decoronated and restored with composite; and III as II supported by fiber posts. In group IV, implants with individual zirconia abutments were used. Specimens were restored with zirconia 2U-FPDs and exposed to two sequences of thermal cycling and mechanical loading. STATISTICS: Kaplan-Meier; log-rank tests. RESULTS: During TCML in group I two tooth fractures and two debondings with chipping were found. Solely chippings occurred in groups II (2×), IV (2×), and III (1×). No significant different survival was found for the different abutments (p = 0.085) or FPDs (p = 0.526). Load capability differed significantly between groups I (176 N) and III (670 N), and III and IV (324 N) (p < 0.024). CONCLUSION: Within the limitations of an in vitro study, it can be concluded that zirconia-framework 2U-FPDs on decoronated teeth with/without post showed comparable in vitro reliability as restorations on implants. The results indicated that restorations on teeth with only access cavity perform worse in survival and linear loading. CLINICAL RELEVANCE: Even severe defects do not justify per se a replacement of this particular tooth by a dental implant from load capability point of view.

Two-body wear of occlusal splint materials.

This study investigates the wear resistance of four different types of occlusal splint materials based on two-body wear simulations under wet and dry conditions. Twenty specimens of each splint material (Dentalon Plus, Orthoplast, Biocryl C, and Eclipse), each with a diameter of 16 mm and a thickness of 3 mm, were tested, half under wet and half under dry conditions. Each wear test was performed using a device called chewing simulator CS-4 (n=10; test load: 50 N; number of cycles: 10000, 20000, and 30000; continuous rinsing with 30°C water for wet conditions); the antagonists were simulated using steel balls. Wear was determined using a 3D laser scanner and a surface analysis program. To detect significant statistical differences, wear data after 10000; 20000; and 30000 cycles were compared using the Kruskal-Wallis test and the Mann-Whitney U-test. The level of significance was set at 5%. Significant differences were found between the groups of different materials tested under wet conditions (P<0.05), whereas no differences between them were found under dry conditions (P>0.05). No significant difference was found between the wet and dry conditions for all materials and cycles (P>0.05). For groups of different materials tested under wet conditions, the degree of volume loss generated in the Chewing Simulator CS-4 was found to differ significantly for different numbers of cycles. The presence of water had no effect on the volume loss in the different material groups that were tested.

Use of a modified shell technique for three-dimensional bone grafting: description of a technique.

BACKGROUND: Defects of the alveolar crest often lead to three-dimensional bone loss after tooth extraction. Therefore, hard tissue grafting is required prior to implant placement. Different techniques have been described in the literature. METHODS: In this case report three-dimensional hard tissue grafting was performed with a modified shell technique and autogenous bone harvested from the mandibular ramus. The shells were trimmed to a thickness of 1 mm and placed to recontour the ideal shape of the alveolar ridge. The shells were then fixed with micro titanium screws, and the gap between the shells and the alveolar ridge was filled with autogenous bone chips. RESULTS: Wound healing was uneventful. Consolidation of the bone graft showed almost no resorption and the implant was placed into vital bone. CONCLUSIONS: The described shell technique for rebuilding three-dimensional alveolar defects showed promising results and could be an alternative treatment to other hard tissue grafting techniques.

Enhancing the predictability of complex rehabilitation with a removable CAD/CAM-fabricated long-term provisional prosthesis: a clinical report.

Implementing any definitive prosthetic treatment of the residual edentulous ridge involves several risks. The patient's expectations may not be completely fulfilled as such treatment procedures include major changes in function and esthetics. Innovative materials, such as high-density polymers based on a highly cross-linked polymethylmetacrylate (PMMA) or composite resin for CAD/CAM-manufacturing are an alternative treatment option. They enhance the predictability of complex rehabilitations, especially in situations where the prognosis of the residual ridge behavior over time is challenging. This article describes an innovative approach of an extended pretreatment phase by using intraoral scanning and CAD/CAM technology for the fabrication of a long-term provisional removable dental prosthesis made of high-density polymer.

Self-adhesive cements as core build-ups for one-stage post-endodontic restorations?

AIM: To investigate the load capability of root filled teeth restored with glass fibre posts when the same self-adhesive composite resin cement was used as post cement and core build-up material. METHODOLOGY: Human maxillary central incisors were divided into four groups (n=10). Teeth were root filled, decoronated and restored using glass fibre posts luted with different cements and composite resins for core build-up (i) RelyX Unicem/Clearfil Core (RXU/CC), (ii) RelyX Unicem/ RelyX Unicem (RXU/RXU), (iii) RelyX Unicem/LuxaCore-Dual (RXU/LCD) and (iv) LuxaCore-Dual/Clearfil (LCD/CC). A 2-mm ferrule crown preparation was always performed. All specimens were restored with adhesively luted all-ceramic crowns and were exposed to thermal cycling and mechanical loading (TCML) and subsequently statically loaded. For analysis of cycles-to-failure during TCML, log-rank statistics were calculated. The nonparametric Kruskal-Wallis test was applied to study group mean differences. Differences in the frequency of the failure modes between the groups were evaluated by Fisher's exact test. All tests were two-sided (α=0.05). RESULTS: Three specimens of RXU/LCD and two of RXU/RXU and LCD/CC, respectively, failed during TCML (P=0.379). For these specimens, the load capability value was set at 0 N. The median fracture load values (min/max) in (N) were RXU/CC=294 (209/445), RXU/RXU = 166 (0/726), RXU/LCD=241 (0/289) and LCD/CC=200 (0/371) (P=0.091). The RXU/CC had the highest (80%) and RXU/LCD the lowest (20%) percentage of restorable failures (P=0.028). CONCLUSIONS: These results imply that self-adhesive composite achieved similar load capabilities when used as core build-up materials in root filled teeth restored with glass fibre posts and all-ceramic crowns.

In vitro performance of self-adhesive resin cements for post-and-core build-ups: influence of chewing simulation or 1-year storage in 0.5% chloramine solution.

The aim of this investigation was to test the in vitro performance of a self-adhesive resin composite core build-up in comparison with two typical conventional etch-and-rinse composite core build-up materials, before and after 1year of storage in 0.5% chloramine solution (LTS). Sixty human maxillary central incisors were divided into three groups. Teeth were root filled and decoronated. Specimens were restored using glass fiber posts cemented with a self-adhesive resin cement. Core build-ups were made with a self-adhesive (U) and two core build-up materials (C and L) applied with their corresponding bonding systems. All specimens received adhesively luted lithium disilicate crowns. Ten specimens of each group were exposed to LTS and examined monthly for cracks or other alterations. All specimens were thermocycled, mechanically loaded (TCML) and finally loaded until failure occurred. There was no statistical significant difference in regard to the number of failures during TCML without and with LTS (log rank: p = 0.225 and 0.609, respectively). The median fracture load values after static loading without LTS and with LTS did not differ significantly (Kruskal-Wallis test: p = 0.057 and 0.106, respectively), though the fracture patterns between the groups without (p = 0.024) and with LTS (p = 0.027) did. Self-adhesive cements used for core build-up have no significantly higher risk of failure compared to conventional core build-up materials in both LTS and TCML test scenarios.

Marginal fit of 14-unit zirconia fixed dental prosthesis retainers.

The marginal fit of 14-unit fixed dental prosthesis retainers and single-crown copings fabricated by the same computer-aided design (CAD)/computer-aided manufacturing (CAM) system were evaluated in vitro hypothesizing that the marginal opening might be independent of the type of restoration. Eight ivorine maxillary teeth (FDI locations 27, 25, 23, 21, 11, 13, 15 and 17) were prepared to accommodate a 14-unit prosthesis. Ten fixed dental prosthesis retainers and 40 single-crown-copings were fabricated using Zeno CAD/CAM on 20 master dies. Four cross-sections were made from each tooth and the marginal gap dimensions were measured. One-way anova was used to test the difference between the experimental groups and two-way anova and a post hoc test (Student-Newman-Keuls) were carried out to determine the influence of the location of the abutment tooth and the measurement location within the abutment tooth in the experimental groups (P < 0.05). Mean marginal gap dimensions and standard deviations for fixed dental prosthesis retainers and single-crown copings were 25 +/- 29 and 13 +/- 12 microm, respectively. The type of restoration showed a significant influence (P < 0.001) on the marginal gap. The location of the abutment tooth (P < 0.001) and the measurement location (P < 0.001) exhibited significant influence on marginal gaps of fixed dental prosthesis retainers while no influence on single-crown copings could be detected. The highest marginal gaps were found at the palatal surface of the incisor and canine of the 14-unit fixed dental prosthesis retainers. Fourteen-unit fixed dental prosthesis showed significantly higher marginal openings than single crowns fabricated under the same conditions. However, both restorations showed clinically acceptable marginal openings.

Digital dentistry: an overview of recent developments for CAD/CAM generated restorations.

As in many other industries, production stages are increasingly becoming automated in dental technology. As the price of dental laboratory work has become a major factor in treatment planning and therapy, automation could enable more competitive production in high-wage areas like Western Europe and the USA. Advances in computer technology now enable cost-effective production of individual pieces. Dental restorations produced with computer assistance have become more common in recent years. Most dental companies have access to CAD/CAM procedures, either in the dental practice, the dental laboratory or in the form of production centres. The many benefits associated with CAD/CAM generated dental restorations include: the access to new, almost defect-free, industrially prefabricated and controlled materials; an increase in quality and reproducibility and also data storage commensurate with a standardised chain of production; an improvement in precision and planning, as well as an increase in efficiency. As a result of continual developments in computer hardware and software, new methods of production and new treatment concepts are to be expected, which will enable an additional reduction in costs. Dentists, who will be confronted with these techniques in the future, require certain basic knowledge if they are to benefit from these new procedures. This article gives an overview of CAD/CAM-technologies and systems available for dentistry today.