Phytotoxicity of zinc and manganese to seedlings grown in soil contaminated by zinc smelting.

Historic emissions from two zinc smelters have injured the forest on Blue Mountain near Palmerton, Pennsylvania, USA. Seedlings of soybeans and five tree species were grown in a greenhouse in a series of mixtures of smelter-contaminated and reference soils and then phytotoxic thresholds were calculated. As little as 10% Palmerton soil mixed with reference soil killed or greatly stunted seedlings of most species. Zinc was the principal cause of the phytotoxicity to the tree seedlings, although Mn and Cd may also have been phytotoxic in the most contaminated soil mixtures. Calcium deficiency seemed to play a role in the observed phytotoxicity. Exposed soybeans showed symptoms of Mn toxicity. A test of the effect of liming on remediation of the Zn and Mn phytotoxicity caused a striking decrease in Sr-nitrate extractable metals in soils and demonstrated that liming was critical to remediation and restoration.

Zinc and lead poisoning in wild birds in the tri-state mining district (Oklahoma, Kansas, and Missouri).

The Tri-State Mining District (Oklahoma, Kansas, and Missouri) is contaminated with Pb, Cd, and Zn from mining, milling and smelting. Metals have been dispersed heterogeneously throughout the District in the form of milled mine waste ("chat"), as flotation tailings and from smelters as aerial deposition or slag. This study was conducted to determine if the habitat has been contaminated to the extent that the assessment populations of wild birds are exposed to toxic concentrations of metals. American robins (Turdus migratorius), northern cardinals (Cardinalis cardinalis), and waterfowl had increased Pb tissue concentrations (p < 0.05) compared with Pb tissue concentrations from reference birds, and the exposure of songbirds to Pb was comparable with that of birds observed at other sites severely contaminated with Pb. Mean activities of the Pb-sensitive enzyme delta-aminolevulinic acid dehydratase (ALAD) were decreased by >50% in red blood cells in these birds (p < 0.05). Several birds had tissue concentrations of Pb that have been associated with impaired biological functions and external signs of poisoning. Cadmium was increased in kidneys of songbirds (p < 0.05), but no proximal tubule cell necrosis associated with Cd poisoning was observed. Zinc concentrations in liver and kidney of waterfowl were significantly higher (p < 0.05) than reference values. The increased environmental concentrations of Zn associated with mining in the District accounted for the pancreatitis previously observed in five waterfowl from the District. The District is the first site at which free-flying wild birds have been found to be suffering severe effects of Zn poisoning.

Toxicity of lead-contaminated sediment to mute swans.

Most ecotoxicological risk assessments of wildlife emphasize contaminant exposure through ingestion of food and water. However, the role of incidental ingestion of sediment-bound contaminants has not been adequately appreciated in these assessments. This study evaluates the toxicological consequences of contamination of sediments with metals from hard-rock mining and smelting activities. Lead-contaminated sediments collected from the Coeur d'Alene River Basin in Idaho were combined with either a commercial avian maintenance diet or ground rice and fed to captive mute swans (Cygnus olor) for 6 weeks. Experimental treatments consisted of maintenance or rice diets containing 0, 12 (no rice group), or 24% highly contaminated (3,950 microg/g lead) sediment or 24% reference (9.7 microg/g lead) sediment. Although none of the swans died, the group fed a rice diet containing 24% lead-contaminated sediment were the most severely affected, experiencing a 24% decrease in mean body weight, including three birds that became emaciated. All birds in this treatment group had nephrosis; abnormally dark, viscous bile; and significant (p

Estimating toxic damage to soil ecosystems from soil organic matter profiles.

Concentrations of particulate and total organic matter were measured in upper soil profiles at 26 sites as a potential means to identify toxic damage to soil ecosystems. Because soil organic matter plays a role in cycling nutrients, aerating soil, retaining water, and maintaining tilth, a significant reduction in organic matter content in a soil profile is not just evidence of a change in ecosystem function, but of damage to that soil ecosystem. Reference sites were selected for comparison to contaminated sites, and additional sites were selected to illustrate how variables other than environmental contaminants might affect the soil organic matter profile. The survey was undertaken on the supposition that environmental contaminants and other stressors reduce the activity of earthworms and other macrofauna, inhibiting the incorporation of organic matter into the soil profile. The profiles of the unstressed soils showed a continuous decrease in organic matter content from the uppermost mineral soil layer (0-2.5 cm) down to 15 cm. Stressed soils showed an abrupt decrease in soil organic matter content below a depth of 2.5 cm. The 2.5-5.0 cm layer of stressed soils--such as found in a pine barren, an orchard, sites contaminated with zinc, and a site with compacted soil--had less than 4% total organic matter and less than 1% particulate organic matter. However, damaged soil ecosystems were best identified by comparison of their profiles to the profiles of closely matched reference soils, rather than by comparison to these absolute values. The presence or absence of earthworms offered a partial explanation of observed differences in soil organic matter profiles.

Lead poisoning of passerines at a trap and skeet range.

Our objective was to determine if ground foraging passerines in a woodland surrounding a trap and skeet range were subject to lead poisoning. Lead availability to birds was determined by shot counts and soil and earthworm analysis. Avian exposure to lead was identified by measuring free-erythrocyte protoporphyrin levels in blood and lead in tissues of three passerine species. Results showed that most shot were found in the top 3 cm of soil. Lead measurements ranged from 110 to 27,000 ppm (dry wt) in soil and were 660 and 840 ppm in earthworms. Sparrows held in an aviary at the range (p=0.02) and free-flying juncos (p=0.0005) mist-netted at the range displayed significantly higher protoporphyrin levels than those at an uncontaminated site. Sparrow and cowbird carcasses from the aviary carried 37 and 39 ppm lead (dry wt), respectively, whereas a junco liver contained 9.3 ppm lead.

Metal and sediment ingestion by dabbling ducks.

The chemical analysis of intestinal digesta from hunter-killed carcasses or of wildlife scat is a promising means of estimating the exposure of wildlife to those environmental contaminants that, like lead, are poorly absorbed in the digestive tract. When evaluating contaminants at a site, biologists may find the results of this non-destructive approach more straightforward to interpret in terms of exposure to wildlife than would be analyses of soils, sediments, water, or wildlife tissues. To illustrate the approach, we collected digesta from 47 waterfowl shot by hunters at Prime Hook National Wildlife Refuge, in Delaware, USA. The waterfowl digesta contained an average of approximately 2.4% sediment, estimated from the Al concentrations in the digesta, a marker for sediment. Al concentrations were significantly correlated with concentrations of Cr (Spearman's rank correlation coefficient, r = 0.57), V (r = 0.70), Ni (r = 0.31), and Pb (r = 0.55), and we concluded that these metals were ingested mainly with sediment. American widgeon (Anas americana) ingested sediment at a rate of about four times that of three other species of dabbling ducks (Anas crecca, A. acuta, A. rubripes) and had several times the exposure to the sediment-associated metals. The digesta of one American black duck contained a high concentration of lead (70 mg/kg, dry wt.), presumably from lead shot, but none of the other samples had notably elevated metal concentrations. We suggest that scat and digesta be analyzed more widely by biologists and resource managers seeking a simple, inexpensive assessment of contaminants in local wildlife habitat.

Retrospective study of the diagnostic criteria in a lead-poisoning survey of waterfowl.

Between 1983 and 1986 the National Wildlife Health Center (NWHC) conducted a nationwide study of lead poisoning of waterfowl from federal and state refuges. This survey was done to assist in identifying zones with lead-poisoning problems. One thousand forty one moribund or dead waterfowl were collected and examined. The presence or absence of 13 gross lesions selected as indicators of lead poisoning and three lesions indicating body condition was recorded. Lead-poisoning diagnoses were based on the finding of at least 6-8 ppm (wet weight) lead in the liver and either lead shot in the gizzard content or at least one convincing gross lesion indicative of lead poisoning. Four hundred twenty-one of these waterfowl were diagnosed as lead poisoned. The NWHC survey provided a comprehensive basis for estimating the sensitivities, specificities, and likelihood ratios of the gross lesions of lead poisoning and the associated hepatic lead concentrations for several species of waterfowl. Some of the 13 defined gross lesions were more common than others; frequencies ranged from 3% to 80% in the 421 lead-poisoned waterfowl. The most reliable indicators of lead poisoning were impactions of the upper alimentary tract, submandibular edema, myocardial necrosis, and biliary discoloration of the liver. Each of the 13 lesions occurred more frequently in the lead-poisoned birds, but each of the lesions also occurred in waterfowl that died of other causes. The number of lead shot present in a bird's gizzard was only weakly correlated with its hepatic lead concentration; however, this weak correlation may have been adequate to account for differences in hepatic lead concentrations among species, once the weights of the species were taken into account. Although lead-poisoned ducks tended to have higher hepatic mean lead concentrations than did lead-poisoned geese or swans, the differences were probably a result of a greater dose of shot per body weight than to kinetic differences between species. Hepatic lead concentrations were independent of age and sex. Ninety-five percent of waterfowl diagnosed as lead poisoned had hepatic lead concentrations of at least 38 ppm dry weight (10 ppm wet weight). Fewer than 1% of the waterfowl that died of other causes had a concentration that high. This fifth percentile, of 38 ppm dry weight (10 ppm wet weight), is a defensible criterion for identifying lead-poisoned waterfowl when interpreting hepatic lead concentrations in the absence of pathological observations.

Survey and evaluation of contaminants in earthworms and in soils derived from dredged material at confined disposal facilities in the Great Lakes Region.

Soils derived from dredged material were collected, together with earthworms from nine confined disposal facilities located in the Great Lakes Region. These samples were analyzed for 18 elements, 11 organochlorine pesticides, PCBs, and 24 polycyclic aromatic hydrocarbons. The concentrations detected in earthworms were evaluated in terms of their potential hazard to wildlife, which for the sake of the evaluation were assumed to prey entirely either on earthworms or on other soil invertebrates having similar concentrations. The soil concentrations (dry wt.) of the contaminants of greatest concern were <1.9 to 32 ppm Cd, <0.053 to 0.94 ppm Hg, 4.6 to 550 ppm Pb, and <0.1 to 1.0 ppm PCBs. The concentrations in earthworms (dry wt., ingested soil included) were as high as 91 ppm Cd, 1.6 ppm Hg, 200 ppm Pb, and 1.8 ppm PCBs. Based on laboratory toxicity studies of relatively sensitive species, and on concentration factors calculated from the earthworm and soil data, we estimated that lethal or serious sublethal effects on wildlife might be expected at concentrations of 10 ppm Cd, 3 ppm Hg, 670 ppm Pb, and 1.7 ppm PCBs in alkaline surface soils derived from dredged material. Concentrations of polycyclic aromatic hydrocarbons in earthworms were well below those in soil.

Trace elements in soil and biota in confined disposal facilities for dredged material.

We studied the relation of trace element concentrations in soil to those in house mice (Mus musculus), common reed (Phragmites australis) and ladybugs (Coccinella septempunctata at five disposal facilities for dredged material. The sites had a wide range of soil trace element concentrations, acid soils and a depauperate fauna. They were very poor wildlife habitat because they were dominated by the common reed. Bioassay earthworms exposed to surface soils from three of the five sites died, whereas those exposed to four of five soils collected a meter deep survived, presumably because the deeper, unoxidized soil, was not as acid. Concentrations of Ni and Cr in the biota from each of the sites did not seem to be related to the concentrations of the same elements in soil. Although Pb, Zn and Cu concentrations in biota were correlated with those in soil, the range of concentrations in the biota was quite small compared to that in soil. The concentrations of Pb detected in mice were about as high as the concentrations previously reported in control mice from other studies. Mice from the most contaminated site (530 ppm Pb in soil) contained only slightly more Pb (8 ppm dry wt) than did mice (2-6 ppm dry wt) from sites containing much less Pb (22-92 ppm in soil). Despite the acid soil conditions, very little Cd was incorporated into food chains. Rather, Cd was leaching from the surface soil. We concluded that even the relatively high concentrations of trace elements in the acid dredged material studied did not cause high concentrations of trace elements in the biota.

Accumulation of lead and organochlorine residues in captive American kestrels fed pine voles from apple orchards.

Pine voles (Microtus pinetorum) were collected from pesticide-treated orchards in New York (USA) and fed to three captive American kestrels (Falco sparverius) for 60 days to evaluate potential hazards from soil-borne persistent insecticides. Three control kestrels were fed uncontaminated laboratory mice (Mus musculus). The pine voles contained an average of 38 ppm lead, 48 ppm DDE and 1.2 ppm dieldrin (wet weight). The kestrels accumulated sublethal amounts of lead (1 ppm lead wet weight) in their livers. In contrast, DDE and dieldrin accumulated in the tissues and brains of kestrels to toxicologically significant concentrations. Control kestrels remained healthy and accumulated insignificant concentrations of the contaminants. The results indicated raptors may not be significantly at risk from lead residues in soil and biota following field applications of lead arsenate. However, sublethal effects may be expected from the level of contamination by organochlorine pesticides.

A survey of Pb, Cu, Zn, Cd, Cr, As, and Se in earthworms and soil from diverse sites.

Earthworms and soils were collected from 20 diverse sites in Maryland, Pennsylvania, and Virginia, and were analyzed for Pb, Cu, Zn, Cd, Cr, As, and Se. Correlation coefficients relating concentrations of the elements in earthworms to concentrations in soil were low (-0.20

Heavy metals in white-tailed deer living near a zinc smelter in Pennsylvania.

White-tailed deer (Odocoileus virginianus (Zimmermann) shot within 20 km of the zinc smelters in the Palmerton, Pennsylvania area contained extremely high renal concentrations of cadmium (372 ppm dry weight (dw] and zinc (600 ppm dw). The deer with the highest renal zinc concentration was shot 4 km from the smelters and had joint lesions similar to those seen in zinc-poisoned horses from the same area. The highest concentrations of lead in both hard and soft tissues were relatively low, 10.9 ppm dw in a sample of teeth, 17.4 ppm dw in a metacarpus, and 4.9 ppm dw in a kidney.

Organochlorine pesticide residues in moths from the Baltimore, MD-Washington, D.C. area.

Moths were collected with a light trap from 15 sites in the Baltimore, Maryland-Washington, D.C. area and analyzed for organochlorine pesticide residues. On the average, the species sampled contained 0.33 ppm heptachlor-chlordane compounds, 0.25 ppm DDE, and 0.11 ppm dieldrin. There were large differences in the concentrations detected in different species. Concentrations were especially high in moths whose larvae were cutworms, and were virtually absent from moths whose larvae fed on tree leaves. It was concluded that at least some species sampled could be an important source of insecticides to insectivorous wildlife. In some instances moths may be useful indicators of environmental contamination, especially when insectivorous wildlife species cannot be collected. However, the differences in residues observed among species means that only similar species should be compared, and this limits their potential for monitoring.