RESUMO
Using the phenomenon of the formation of the active forms of oxygen by phagocytes in the process of adhesion, changes occurring in the cell membrane under the influence of sodium caseinate, macrophage migration inhibition factor, aggregated IgG and hydrolytic enzymes could be registered. Sodium caseinate inhibited chemiluminescence during the first 10 minutes in the adhesion of mouse peritoneal macrophages, human lung macrophages and adhesive marrow cells, while large doses of this preparation activated chemiluminescence at the latter stages of adhesion. Macrophage migration inhibition factor induced the rapid and prolonged formation of the active forms of oxygen in the process of macrophage adhesion, aggregated IgG decreased this cell function, and the subsequent phagocytosis of zymosan was accompanied by increased chemiluminescence. The experimental and practical possibilities of this phenomenon are discussed.
Assuntos
Macrófagos/fisiologia , Animais , Adesão Celular/efeitos dos fármacos , Humanos , Medições Luminescentes , Pulmão/citologia , Ativação de Macrófagos/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Neutrófilos/fisiologia , Consumo de Oxigênio/efeitos dos fármacos , Cavidade Peritoneal/citologia , Fagocitose/efeitos dos fármacos , Propriedades de SuperfícieRESUMO
The method for the evaluation of the chemotaxis of neutrophils in the presence of agarose is presented, its advantages and some methodological aspects are discussed, and the detailed description of this method as developed by the authors is given.
Assuntos
Quimiotaxia de Leucócito/efeitos dos fármacos , Fagócitos/efeitos dos fármacos , Sefarose/farmacologia , Animais , Separação Celular , Géis , Humanos , Métodos , Camundongos , Neutrófilos/efeitos dos fármacos , CoelhosRESUMO
Secretions of hormones responsible for carbohydrate metabolism, STH, insulin, S-peptide and somatostatin, were measured in coronary patients in order to investigate hormonal mechanisms involved in disordered carbohydrate metabolism. A persistent hyperinsulinemia was found in patients with disordered carbohydrate metabolism, both in cases of glucose stimulation and insulin inhibition, where glandular function was assessed on the basis of S-peptide concentration as opposed to the control level. Baseline somatostatin concentrations were beyond the method's sensitivity limit in most of the patients. There was a tendency to elevated hormonal levels in the insulin test, whereas glucose administration produced an opposite response. Blood basal STH levels did not differ significantly in the study groups, however the increase in hormonal secretion following insulin administration was less pronounced in patients with disordered carbohydrate metabolism as compared to other groups.