RESUMO
Objective: To investigate the effect of transcription factor E2F1 on the invasion of prostate cancer and its clinical significance. Methods: A stable inhibition of E2F1 prostate cancer cell line PC3 was established. The E2F1 and relative invasion biomarker protein expression level of the transfected cells was detected by Western blot. The PC3 cells were divided into two groups: the control group and sh-E2F1 group, cell invasion assay and cell scratch test were used to detect the number of cell migration in the experiment time. The relationship between E2F1 mRNA expression level and clinical prognostic parameters was analyzed through microarray data of prostate cancer. Results: E2F1 inhibited PC3 cell line was constructed successfully. The results of Western blot showed that the expression of Vimentin, CD147, MMP-2 and MMP-9 protein in E2F1 suppression group was lower than those in control group, while the protein expression of E-cadherin increased. Compared to the control group, inhibiting the expression of E2F1 in prostate cancer cells significantly decreased the invasion and migration, with significant difference (P<0.05). High mRNA expression of E2F1 decreased biochemical recurrence rate and overall survival rate after surgery (P=0.047, 0.035), and the E2F1 expression level was related to pathological stage of prostate cancer. Gleason score and tumor metastasis (P<0.05). Conclusion: E2F1 enhances the invasion and metastasis of prostate cancer through a variety of mechanisms, and its expression level has an important relationship with the adverse prognosis of patients with prostate cancer.
