RESUMO
The cultivation was done on Eagle's media as well as Eagle's media enriched with the supplement of 10 and 20% of bovine serum. In the media without serum the larvae lived significantly shorter and did not get through molting. They lived the longest and came through molting the fastest in the media with pH 2.0 supplemented with 20% SB. In the media with the pH 4.0 and 7.3 the larvae lived significantly shorter and only a small proportion reached stage IV.
Assuntos
Anisakis/crescimento & desenvolvimento , Meios de Cultura/química , Meios de Cultura/metabolismo , Larva/crescimento & desenvolvimento , Parasitologia/métodos , Animais , Bovinos , Peixes/parasitologia , Crescimento e Desenvolvimento , Concentração de Íons de Hidrogênio , Muda , Movimento/fisiologia , Taxa de SobrevidaRESUMO
The objective of this work was to examine the influence of heavy metals ions, zinc and lead, in external environment on the development of Ascaris suum to invasive stage. The culture mediums contained 25.5, and 0.5 mM Pb(NO3)2 or ZnSO4. After 6 weeks of development in 26 degrees C in the control 75.17% of eggs reached the invasive stage. Ions of lead and zinc, in a way depending on concentration inhibited development of embryos. The invasive stage in the presence of 25.5 and 0.5 mM Pb(NO3)2 were achieved by 37.89% , 44,75% and 64.63% of eggs and in ZnSO4 solutions with the same concentrations of ions 45.40%, 50.36% and 66.61% respectively. Many of eggs in 25 mM solutions of both metals stopped at the blastula stage.
