Molecular prevalence, epidemiology, and phylogenetic analysis of Babesia microti in dogs with a note on its impact on host hematological profile.

Babesia (B.) microti is an intra-erythrocytic protozoan parasite that infects humans as well as domestic and wild animals. Prevalence of B. microti was investigated in 654 apparently healthy dogs belonging to 55 different breeds from three districts in Punjab province (Muzaffargarh, Bahawalpur and Jhang) and two districts in Khyber Pakhtunkhwa province (Dir Upper and Charsadda) in Pakistan. The hematological profile of dogs, risk factors associated with the infection and phylogenetic diversity of the detected isolates were also evaluated. In total, 29 blood samples (4 %) scored PCR positive. Sanger sequencing of partial 18S rRNA gene confirmed the presence of B. microti. The phylogenetic analysis of the sequences based on the 18S rRNA gene displayed global phylogenetic similarity with the isolates that were previously documented from Russia, France, Poland, Spain, China, Japan and USA. The infection rate was consistent across different sampling sites and dog breeds. Sex or presence of ectoparasites on dog was also not associated with B. microti prevalence. Babesia microti infected dogs had elevated red cell distribution width-coefficient of variation (%) than uninfected animals. This study presents updated data about the prevalence of B. microti among local Pakistani dogs and will be helpful in designing control strategies against this tick-borne pathogen as the tick infesting a B. microti infected dog may transmit this parasites to human as well.

Diversity, Distribution, and applications of arbuscular mycorrhizal fungi in the Arabian Peninsula.

Investigations of arbuscular mycorrhizal fungi (AMF) received extreme interests among scientist including agronomists and environmental scientists. This interest is linked to advantages provided by AMF in enhancing the nutrients of their hosts via improving photosynthetic pigments and antioxidant production. Further, it also positively alters the production of plant hormones. AMF through its associations with plants obtain carbon while in exchange, provide nutrients. AMF have been reported to improve the growth of Tageteserecta, Zea mays, Panicum turgidum, Arachis hypogaea, Triticum aestivum and others. This review further documented the occurrence, diversity, distribution, and agricultural applications of AMF species reported in the Arabian Peninsula. Overall, we documented 20 genera and 61 species of Glomeromycota in the Arabian Peninsula representing 46.51 % of genera and 17.88 % of species of AMF known so far. Funneliformis mosseae has found to be the most widely distributed species followed by Claroideoglomus etuicatum. There are 35 research articles focused on Arabian Peninsula where the stress conditions like drought, salinity and pollutants are prevailed. Only one group studied the influence of AMF on disease resistance, while salinity, drought, and cadmium stresses were investigated in 18, 6, and 4 investigations, respectively. The genus Glomus was the focus of most studies. The conducted research in the Arabian Peninsula is not enough to understand AMF taxonomy and their functional role in plant growth. Expanding the scope of detection of AMF, especially in coastal areas is essential. Future studies on biodiversity of AMF are essential.

Isolation and evaluation of Qatari soil rhizobacteria for antagonistic potential against phytopathogens and growth promotion in tomato plants.

Plant growth promoting rhizobacteria are a diverse group of microorganisms that enhance the growth of plants under various conditions. In this study, 55 isolates of endogenous rhizobacteria were collected from the rhizosphere of Avicennia marina, Suaeda vermiculata, Salsola soda, Anabasis setifera, Salicornia europaea, Arthrocnemum macrostachyum, Limonium axillare, Tetraena qatarensis, Aeluropus lagopoides, and Prosopis juliflora. The isolates were evaluated in-vitro for their antagonist potential against Fusarium oxysporum and Botrytis cinerea using the dual culture technique, where the maximum growth inhibition reached 49% and 57%, respectively. In-vivo evaluation was accomplished to determine the growth-promoting potential of the rhizobacteria under greenhouse conditions where the strain ANABR3 (Bacillus subtilis) showed the strongest growth-promoting effects. Further in-vivo testing regarding the effectiveness of rhizobacteria in the presence of the phytopathogen was also completed using the Hoagland medium. LEMR3 and SALIR5 (both identified as two strains of B. subtilis) supported the tomato seedlings to overcome the disease and significantly (p ≤ 0.05) increased above and belowground biomass compared to the control. Additionally, several characterizing tests were carried out on the selected strains, these strains were found to possess numerous features that promote plant growth directly and indirectly such as the production of IAA, HCN, hydrolytic enzymes, ACC deaminase, NH3, and some rhizobacteria were capable of phosphate solubilization. In conclusion, this study showed that local rhizobacterial isolates collected from arid lands possess valuable traits, making them promising bio-control agents and bio-fertilizers for agricultural purposes.

Quality of Neonatal Care: A Health Facility Assessment in Balochistan Province, Pakistan.

Introduction Balochistan is the largest of Pakistan's four provinces, yet it is also the poorest and most impoverished, particularly in terms of neonatal healthcare. In order to build and tailor strategies to improve neonatal outcomes, it is necessary to identify barriers and facilitators for interventions. Therefore, we conducted this study to provide an overview of neonatal healthcare quality and assess the structural capacity for the improvement and further development of neonatal healthcare facilities in Balochistan. Methods A descriptive, observational, cross-sectional study was conducted in Balochistan, a province of Pakistan. The survey was designed to assess the level of staffing and facilities in the neonatal health care units. Data were gathered through trained staff either by in-person visits to the facility or via telephone. Results A total of 177 facilities were assessed in 25 districts of Balochistan. A majority (88.7%) of the facilities were from the public sector. Birth and neonatal care services were provided at only 63 (36%) of the assessed facilities and only three had newborn intensive care units (NICUs) with a 1:5 staff: patient ratio. Unfortunately, all NICUs lacked the basic advanced facilities. None of the hospitals had an infection control policy or staff nor any training program for doctors. Conclusion In conclusion, healthcare facilities to manage neonatal patients requiring hospital care are extremely limited in Balochistan and the ones that are available have very limited resources. To improve the healthcare system in Balochistan, all stakeholders should be involved in the planning, decision-making, and implementation of healthcare programs at all levels to ensure sustainability and efficiency.

Laboratory Blood Testing In Paediatric Intensive Care Unit Of A University Hospital: Are We Doing It Appropriately?

Retrospective chart review of all children (aged-one month to 16 years) admitted in our paediatric intensive care from June to November 2016 was done to determine the indication of different laboratory tests. LBT indications were defined into: diagnostic/case findings/screening tests to make a diagnosis; haemostatic tests (to monitor function or identify before clinical signs and symptoms) and therapeutic /monitoring tests to get the level of drug directly or getting level of marker as a guide to therapy. Laboratory tests reports which were within normal range more than once were labelled as in-appropriate tests. In total 274 patients, Haemostatic tests were performed for mean of 35.18±56.72 times (range of 0-429), monitoring for mean of 9.38±20 times (range 0-165), and therapeutic tests (3.26±11.25). Most common tests included serum Sodium levels (7.83±12.73), Serum Potassium (8.19±12.80), bicarbonate (7.75±11.9). 13.40±9.11 tests were done on first day and 13.0±8.49/day tests were performed afterwards. Cumulatively 54.31±74.21 tests were performed/ patients out of which 18.5±37.82 were inappropriate.

Microbially induced calcite precipitation in calcareous soils by endogenous Bacillus cereus, at high pH and harsh weather.

Microbially induced calcite precipitation (MICP) improves the physical properties of soils by increasing the solid content, decreasing the pore sizes and improving the rigidity of the particle-to-particle contact ending with a better mechanical and geotechnical performances of the soils. First, the physical characteristics of soils in Qatar showed similar grain size distributions with most falling in the category of fine particles (30 µm-1 µm), which is appropriate for MICP processes. MICP is ensured by the ureolytic activity of urease producing bacteria. However, Qatari soils are characterized with high carbonate contents exceeding 20%, high alkalinity and fluctuations of temperature and aeration. Although such properties can cause calcite dissolution as reported in literature, two Qatari endogenous B. cereus strains, QBB4 and QBB5, were shown able to adapt to the harsh conditions and induce mineral formations by MICP. Their optimal potentials was at wide ranges of temperature from 30 °C to 42 °C and pH from 7.0 to 8.0. Using Qatari bacteria in liquid cultures and at syringe level at laboratory conditions, as well as in plots at field conditions, 16% CaCO3 increase in minerals formation was obtained, leading to 7% increase of soils stability. Increase of carbonate contents was also shown by an increase in the stability of aggregates to disintegration when incubated in water using a 0.25 mm sieve. MICP in Qatari soils is feasible using B. cereus QBB4, as this is an endogenous bacterium that tolerates harsh conditions, high alkalinity and calcium contents. The performance of these bacterial strains was achieved with clear formation of aragonite, feldspars and quartz in the calcareous soils.

Isolation, differentiation and biodiversity of ureolytic bacteria of Qatari soil and their potential in microbially induced calcite precipitation (MICP) for soil stabilization.

Biomineralization plays a key role in modifying the geological properties of soil, thereby stabilizing it against wind erosion, especially in areas characterized by harsh weather and harsh soil (calcareous and arid); i.e. Arabic Gulf region. Among soil microorganisms, ureolytic bacteria are capable of modifying soil characteristics and thus, inducing biomineralization. This research investigated the occurrence and diversity of ureolytic bacteria in Qatari soils, specifically to study their acquired potential to adapt to harsh conditions exhibiting ureolytic activity. Soil samples were collected from various locations in Qatar and were used to isolate the indigenous ureolytic bacteria. It was noticed that most of the ureolytic bacteria in Qatari soil belong to the genus Bacillus mainly Bacillus cereus. Identification and differentiation of 18 ureolytic isolates were performed using MALDI-TOF MS techniques while ribotyping (16S rRNA) molecular technique was used mainly for 6 selected strains. This study not only shows the diversity of species of ureolytic bacteria in Qatari soil but also shows the diversity in their protein profiles, which confirms that bacteria have adapted well to the harsh environment. In addition, the strains were evaluated based on a newly modified screening method in this work; i.e. production of arbitrary urease activity (AUA). Thus, the strains showing the highest AUA, exhibited the highest capability to produce urease enzymes induced by urea. Analysis of calcium carbonate precipitation utilizing SEM-EDX showed that the ureolytic bacteria also play a significant role in the precipitation of minerals such as CaCO3, in the presence of urea in soil. Therefore, this research showed a high occurrence of indigenous Bacillus bacteria in Qatari soil that can perform biomineralization and thus can be helpful, if properly stimulated, in enhancing soil stabilization, and for other local applications as well, since they are adapted to these soil and weather conditions.

Comparison of the effectiveness of zinc supplementation in tablets form with that of the suspension form in the treatment of acute diarrhoea.

OBJECTIVE: To compare the effectiveness of zinc supplementation in tablet form with that of the suspension form in the treatment of acute diarrhoea. METHODS: A comparative study was carried out at the Liaquat University Hospital, Hyderabad, Pakistan from October 2008 to April 2009, and comprised children aged6-24 months suffering from acute diarrhoea. The patients were divided into two groups on the basis of even and odd numbers. Group A (even numbers) received dispersible zinc tablets, and group B (odd numbers) received zinc suspension. The patients were admitted for 3 days and improvement was checked at the end of 3rd day in terms of decrease in the frequency of stools/day. SPSS 15 was used for data analysis. RESULTS: The 88 patients were divided into two groups of 44(50%) each. Overall, 49(55.7%) patients were male and 39(44.3%) were female. At the end of the 3rd day 51(58%) patients improved, while 37(42%) did not. In the zinc tablet group, improvement was in 32(72%) patients compared to 19(43%) in the zinc suspension group (p<0.05).diarrhoea. CONCLUSIONS: The results of tablets preparation were clinically significant in reducing the duration and severity of diarrhoea.