Biosolids recycling impact on biofilm extracellular enzyme activity and performance of hybrid rotating biological reactors.

Biological processes for wastewater treatment is limited by extracellular enzyme activity (EEA) of the biofilm on polymeric substrates. The efficiency of biodegradation / biosorption mechanisms causing EEA and organic load removal in biofilms remains unknown. Our hypothesis was that the limiting step of biological process can be overcome by biostimulation and/or bioaugmentation of the return sludge in hybrid biofilm reactors, which leads to competition between suspended and attached bacteria and lower effective substrate to microrganism ratio. Therefore, we considered more active biosolids to perform best at enhancing reactor removal rate. To test this, the efficacy of recycling distinct bio-solids types considered to have different bacterial activity such as final effluent (FE), humus solids (HS) and recycle activated sludge (RAS) on performance improvements of rotating biofilm reactors (RBRs). These bio-solids were investigated under high organic loading rates (OLR) and solids loading rates (SLR) using pilot scale reactors receiving real municipal wastewaters. Controlled overloading of RBRs revealed that EEA improved with increasing OLR/SLR. High SLR (>3.3 kg Total Suspended Solids m-2 d-1) delayed and decreased the reduction of organic and inorganic removal rates in the biological processes which commonly occurs under high OLRs. This effect was more pronounced in the highest activity solids (RAS > HS > FE) suggesting the activity and function of bio-solids was critical to improve performance of RBRs. High OLR and SLR induced efficient denitrification and organics removal within the biofilm reactor at residence times of <5 min. Recycling active solids permitted EEA despite overloading which was critical to the performance of the RBRs.

Microbial extracellular enzyme activity affects performance in a full-scale modified activated sludge process.

The rate-limiting step of wastewater treatment is the breakdown of polymers by extracellular enzyme activity (EEA). The efficacy of EEA on biomass from full scale conventional activated sludge (AS) and modified AS with bench scale and full scale rotating biofilm reactors (RBR) was compared. The maximum amino-peptidase EEA was 394 ±â€¯34 µmolL-1 min-1 for the bench RBR which was 11.7 and 4.5 times greater than maximum α-glucosidase and phosphatase EEA in these reactors. At full scale the RBR gave ~4.6, 13.5 and 6.3 times the EEA for amino-peptidase, α-glucosidase and phosphatase (based on enzyme Vmax) compared to the highest EEA in conventional AS biomass. Controlled overloading of the bench RBRs revealed that EEA increased with OLR up to 190 g tCOD m-2d-1 and further increases in OLR reduced the EEA. Pretreatment of wastewater by EEA in the RBR was linked to better performance of the modified activated sludge process. Maintaining high EEA of biofilms is critical for the design of high OLR wastewater treatment systems.