RESUMO
AIM: The aim of this study was to compare the efficacy of CanalBrush (CB), EndoActivator (EA), and Passive Ultrasonic Irrigation (PUI) on the removal of triple antibiotic paste (TAP) from root canal walls. MATERIALS AND METHODS: Thirty-six extracted human single-rooted teeth were prepared using ProTaper Universal rotary files (DentsplyMaillefer, Ballaigues, Switzerland) up to size F5. The root canals were filled with TAP, and after 21 days, roots were randomly assigned to three groups (n = 10) according to irrigation regimens used: CB, EA, and PUI. In three teeth, TAP was not removed (positive controls), and the other three teeth were not filled with TAP (negative controls). The roots were sectioned, and the amount of TAP remaining was evaluated at the mesial halves of each tooth at ×30 magnification under a stereomicroscope using a 4-grade scoring system. Data were evaluated using the Kruskal-Wallis and Mann-Whitney U tests. RESULTS: There were significant differences among the experimental groups according to the different parts of the root canals (P < 0.05). At the apical and middle third, EA and PUI groups removed more TAP than CB group; however, there was a statistically significant difference only between CB and PUI groups (<0.01 at apical third and <0.05 at middle third). At the coronal third, there was no statistically significant difference between all the three groups (P > 0.05). CONCLUSION: PUI led to superior results compared to CB in the middle and apical thirds. There was no significant difference between EA and PUI techniques.
RESUMO
AIM: This review intends to provide a brief overview regarding quorum sensing among bacteria in biofilms and also attempts to throw light on the new research focusing on interference with the quorum sensing. BACKGROUND: Dental plaque is an example of microbial biofilm leading to periodontal disease and dental caries. Quorum sensing is widely employed by a variety of gram-positive and gram-negative bacterial species to coordinate various activities in biofilms. Quorum-sensing-interfering compounds have either a positive or a negative effect on the expression of bacterial phenotypes regulated by quorum sensing. These studies of bacterial quorum sensing have also suggested several ideal targets for drug design which can be promising in preventive and therapeutic aspects of periodontal diseases and dental caries. RESULTS: Studies have shown that periodontal disease and dental caries is caused by plaque biofilm bacteria. Quorum sensing is the means of communication between these bacteria to regulate a wide range of behavior patterns among them. The in vitro studies reviewed here have a vital role in opening up this field, because they reveal the basic machinery of cell--cell signaling in microbial communities. The signal machinery bacteria use to coordinate a variety of their activities is identified by these studies. Further, this review aims to discuss several natural and synthetic methods which were used for manipulating bacterial quorum sensing. CONCLUSION: The future challenge lies in the ability of the dental research to develop additional mechanisms for interfering with bacterial quorum sensing which can be used as preventive and therapeutic tools for combating oral polymicrobial diseases. CLINICAL SIGNIFICANCE: This article aims at reviewing the literature and helping us to understand the ways of communication among bacteria in biofilms, which further open up the prospects in the treatment of diseases caused by biofilms.