RESUMO
The combined approach of classical fingerprinting and DNA profiling is a powerful tool in forensic investigations of latent "touch" traces. However, little attention has been paid to the organic solvents frequently used in dactyloscopic laboratories to facilitate the separation of adhesive evidence prior to fingerprint development and downstream effects on subsequent DNA profiling. In the present study, we tested a selection of adhesive removers (n = 9) and assessed their potential impact on DNA recovery and amplification by PCR. Thereby, we identified and characterized novel PCR inhibitors. All investigated chemicals contain volatile organic compounds that evaporate under normal indoor atmospheric conditions. Exposure to certain solvents resulted in increased DNA degradation, but only if evaporation was prevented. A series of adhesive-removal experiments were conducted with prepared mock evidence (self-adhesive postage stamps affixed to paper envelope) to investigate the impact of treatment time and the location of applied traces on DNA recovery and dactyloscopy, respectively. Due to the early onset of print decomposition, we found that only a short treatment time was compatible with the development of fingerprints on the adhesive side of a stamp. Solvents also removed DNA from the adhesive surface, thus resulting in a marked shift in the substrate distribution of recovered DNA from the stamp to the envelope, but not in the reverse direction. Furthermore, we observed that treatment with conventional fingerprint reagents lead to a significant reduction in the amounts of DNA recovered from stamps, while the additional use of adhesive removers did not significantly enhance this effect.
Assuntos
Adesivos , Dermatoglifia , Humanos , Impressões Digitais de DNA/métodos , Solventes , DNA/análiseRESUMO
Although liposomes have many outstanding features such as biocompatibility, biodegradability, low toxicity and structural diversity, and are successfully applied in many areas of chemistry and biotechnology, a lack of characterization standards and quality control tools are still inhibiting the translation of liposome technology into clinical routine. The greatest obstacle to clinical scale commercialization is the inability to ensure liposome formulation stability because small size variations or altered surface chemistries can significantly influence in vivo distribution and excretion kinetics that could in turn lead to unpredictable therapy outcomes. To enhance the product development process we have developed a microfluidic biochip containing embedded dielectric microsensors capable of providing quantitative results on formulation composition and stability based on the monitoring of the unique electric properties of liposomes. Computational fluid dynamic (CFD) simulations confirmed that microfluidics offer reproducible and well-defined measurement conditions where a moving liposome suspension within a microchannel behaves like a bulk material. Results of this study demonstrate the ability of microfluidics, in combination with dielectric spectroscopy and multivariate data analysis methods, to identify nine different liposomes. We also show that various liposome modifications such as membrane-bound surface proteins, lipid bilayer soluble drugs, as well as protein and dye encapsulations, can be detected in the absence of any labels or indicators. Since shelf-life stability of a liposome formulation is regarded of prime importance for regulatory approval and clinical application, we further provide a possible practical application of the developed liposome analysis platform as a high-throughput tool for industrial quality insurance purposes.
Assuntos
Dispositivos Lab-On-A-Chip , Lipossomos/análise , Técnicas Analíticas Microfluídicas/métodos , Interpretação de Imagem Assistida por Computador , Cinética , Técnicas Analíticas Microfluídicas/instrumentação , Análise Multivariada , Propriedades de SuperfícieRESUMO
Interdigital electrode structures (IDES) play a major role in many technical and analytical applications. In particular, they are a key technology in modern lab-on-a-chip (LOC) devices. As high sensitivity is a key component of any (bio)analytical method, the presented work is aimed at designing a novel dielectric sensing system, which exhibits maximum sensor sensitivity using passivated dielectric microsensors. Although the implementation of high-ε(r) dielectric passivation materials such as tantalum oxide or titanium oxide showed increased sensor sensitivity by a factor of 5, simulations revealed that sensor sensitivity is ultimately determined by the dielectric properties of the analyte. Ideally, dielectric properties of the passivation material need to be adjusted to the dielectric properties of the material under investigation and any deviations (e.g. higher or lower dielectric constants) will result in significant loss of sensitivity. To address these shortcomings we have developed a novel dielectric sensing concept based on a dual-material passivation geometry. The novel design consists of electric flux barriers that are layered between the finger electrodes, as well as electric flux guides which are located above the electrode structures that direct the entire generated electric flux to the object under investigation. Our 3D numerical results clearly show that the novel design offers two main advantages: firstly, the measurement sensitivity is further increased by more than a factor of two in comparison to a homogeneous passivation material sensing strategy. Secondly, maximum sensitivity for a given set of finger geometries can be achieved using a single sensor design regardless of the frequency-dependent dielectric properties of the measured objects. Hence, the novel approach is capable of reducing design and manufacturing costs of lab-on-a-chip devices.
Assuntos
Simulação por Computador , Capacitância Elétrica , Dispositivos Lab-On-A-Chip , Eletrodos , SoftwareRESUMO
Rapid detection of viral contamination remains a pressing issue in various fields related to human health including clinical diagnostics, the monitoring of food-borne pathogens, the detection of biological warfare agents as well as in viral clearance studies for biopharmaceutical products. The majority of currently available assays for virus detection are expensive, time-consuming, and labor-intensive. In the present work we report the creation of a novel micro total analysis system (microTAS) capable of continuously monitoring viral contamination with high sensitivity and selectivity. The specific interaction between shape and surface chemistry between molecular imprinted polymer (MIP) and virus resulted in the elimination of non-specific interaction in the present sensor configuration. The additional integration of the blank (non-imprinted) polymer further allowed for the identification of non-specific adsorption events. The novel combination of microfluidics containing integrated native polymer and MIP with contact-less dielectric microsensors is evaluated using the Tobacco Mosaic Virus (TMV) and the Human Rhinovirus serotype 2 (HRV2). Results show that viral binding and dissociation events can be readily detected using contact-less bioimpedance spectroscopy optimized for specific frequencies. In the present study optimum sensor performance was achieved at 203 kHz within the applied frequency range of 5-500 kHz. Complete removal of the virus from the MIP and device reusability is successfully demonstrated following a 50-fold increase in fluid velocity. Evaluation of the microfluidic biochip revealed that microchip technology is ideally suited to detect a broader range of viral contaminations with high sensitivity by selectively adjusting microfluidic conditions, sensor geometries and choice of MIP polymeric material.
Assuntos
Técnicas Analíticas Microfluídicas/métodos , Impressão Molecular , Polímeros/química , Vírus/isolamento & purificação , Artefatos , Impedância Elétrica , Células HeLa , Humanos , Técnicas Analíticas Microfluídicas/instrumentação , Rhinovirus/isolamento & purificação , Vírus do Mosaico do Tabaco/isolamento & purificaçãoRESUMO
An under recognized cause of preventable mortality is healthcare-associated (nosocomial) infections such as biofilms found on implants and catheters. About 5% of U.S. and E.U. patients acquire nosocomial infections leading to prolonged hospitalization, increased patient suffering, and mortality rates. To date, no satisfactory solutions are available to monitor biofilm formation under near-native conditions. As a consequence, in the present work, we report the development of a disposable microfluidic biochip capable of continuously monitoring cell population dynamics under physiological shear force conditions. We demonstrate the simultaneous application of contactless bioimpedance spectroscopy and amperometric measurements to monitor fungal biofilm growth rates and metabolic activities. Quantitative cell analysis is accomplished by the use of high-density interdigitated capacitors (microIDC) isolated by a 700 nm epoxy (SU-8 resist) based passivation layer to noninvasively assess biofilm formation in predefined proliferation chambers. Additionally, biofilm respiration activity is measured using redox-mediators oxidized at band electrodes located downstream within microchannels. The disposable biofilm analysis platform is used to continuously monitor the dynamic responses of C. albicans to different glucose and galactose concentrations.