[Molecular characterization of Babesia bovis msa-2c gene]. / Babesia bovis'in msa-2c geninin moleküler karakterizasyonu.

OBJECTIVE: This study was carried out to determine the molecular characterization of msa-2c gene of one Babesia bovis isolate from cattle in the Aegean Region and to compare identities with similar isolates from the World and Turkey. METHODS: Between 2008-2010 blood samples were collected from a total of 235 cattle localized in 9 provinces of the Marmara and Aegean Regions. Smears were prepared, genomic DNA's were extracted from the blood samples and investigated for Babesia species by RLB. PCR was performed on one sample determined as B. bovis, the obtained amplicon was purified, sequenced and deposited to GenBank. Identities with similar isolates from Turkey and the World were investigated. RESULTS: Bovine babesiosis was not determined in the microscopic examination. According to the RLB results there was no B. bovis positivity in cattle from the Marmara Region, while only one B. bovis positivity was detected in cattle from the Aegean Region. The molecular prevalence of B. bovis was determined as 0.42% in the total of the examined 235 cattle. The sequenced B. bovis isolate shared 91-92% and 89-96% identities with the isolates from Turkey and the World, respectively. CONCLUSION: Molecular characterization of msa-2c gene region of B. bovis detected from cattle in the Aegean Region was carried out in this study.

[The investigation of fasciolosis in cattle by copro-ELISA and stool examination techniques around the Derinkuyu region]. / Derinkuyu Yöresindeki Sigirlarda Fasciolosisin Kopro-ELISA ve Diski Muayene Yöntemleriyle Arastirilmasi

OBJECTIVE: This study was carried out between September 2008 and June 2009 to determine the prevalence of fasciolosis in cattle in Derinkuyu district of Nevsehir. METHODS: Fecal samples from 198 cattle were technically collected and examined by sedimentation-zinc sulphate flotation technique. Modified McMaster sedimentation technique was applied to the egg positive samples to determine the EPG values. F. hepatica coproantigens in samples were investigated by ELISA. RESULTS: The coprological and antigen ELISA prevalence of fasciolosis were determined as 2.02% and 3.03%, respectively. The mean EPG value in infected cattle was found as 75±22.9. The prevalence of other parasites, Trichostrongylus spp., Eimeria spp., Nematodirus spp., Moniezia spp., Toxocara vitulorum and Ostertagia spp. were determined as 28.3%, 12.6%, 1.5%, 1.0%, 1.0% and 0.5%, respectively. The prevalence of fasciolosis was observed to be higher in the > 3 age group (3.2%) than ≤3 age group (2.9%), however, this difference was not statistically significant (p > 0.05). The prevalence in female and male cattle was found as 3.4% and 4.8% This difference also was not found statistically significant (p > 0.05). The highest prevalence was observed in Fresian with the ratio of 3.5% and this was followed by 2.8% in Rubia Gallega and 2.4% in Brown Swiss. The differences among breeds were not statistically significant (p > 0.05). CONCLUSION: The presence and prevalence of fasciolosis was revealed with this study.

[The molecular diagnosis of Dirofilaria immitis in vector mosquitoes in Felahiye district of Kayseri]. / Kayseri'nin felahiye yöresinde dirofilaria immitis'in vektör sivrisineklerde moleküler biyolojik tanisi

This study was designed to determine the potential vectors of Dirofilaria immitis by molecular techniques in Felahiye district of Kayseri. Mosquitoes were sampled from 11 points between June-August 2008 and collected live samples were brought to laboratory. In order to allow the larval development, mosquitoes were incubated in in vitro conditions for seven days. After this mosquitoes were killed and species identifications were done. Among the totally collected 301 mosquitoes, 96 (31.9%) were belonging to Aedes vexans and 205 (68.1%) to Culex pipiens. Head-thorax and abdomens of each sample were dissected to determine the infective and infected mosquitoes and totally 54 pools (2-17 sample/pool) were constituted according to species and collected region. Genomic DNA was extracted from pools and analyzed by PCR using species specific primers. Dirofilaria immitis DNA was found in 2/54 of the pools formed with one head-thorax and one abdomen pool of Ae. vexans. The minimum infection rate (MIRs) was calculated as 0.33 % in the study area. MIRs of 20 pools consisted from Ae. vexans was determined as 1.04 %. No filarial DNA was detected in 34 pools consisted from Cx. pipiens. Consequently, Ae. vexans was the active potential vector of D. immitis in the study area.