RESUMO
Competency in flexible endoscopy is a major goal of small animal internal medicine residency training programs. Hands-on laboratories to teach entry-level skills have traditionally used anesthetized laboratory dogs (live dog laboratory [LDL]). Virtual-reality endoscopy trainers (VRET) are used for this purpose in human medicine with the clear benefits of avoiding live animal use, decreasing trainee stress, and allowing repeated, independent training sessions. However, there are currently no commercially available veterinary endoscopy simulators. The purpose of the study was to determine whether a human VRET can be a reasonable alternative to a LDL for teaching early veterinary endoscopy skills. Twelve veterinarians with limited or no endoscopy experience underwent training with a VRET (n = 6) or a LDL (n = 6), performed two recorded esophagogastroduodenoscopies (EGD) on anesthetized dogs for evaluation purposes (outcomes laboratory), and then underwent training with the alternative method. Participants completed questionnaires before any training and following each training session. No significant differences were found between training methods based on: measured parameters from the outcomes laboratory, including duration of time to perform EGD; evaluators' assessment of skills; and, assessment of skills through blinded review of the esophageal portion of EGD recordings. The VRET was less stressful for participants than the LDL (p = .02). All participants found that the VRET was a useful and acceptable alternative to the LDL for training of early endoscopy skills. Based on this limited study, VRET can serve as a reasonable alternative to LDL for teaching endoscopy skills to veterinarians.
Assuntos
Simulação por Computador , Educação em Veterinária , Endoscopia , Realidade Virtual , Animais , Competência Clínica , Simulação por Computador/normas , Cães , Educação em Veterinária/métodos , Educação em Veterinária/normas , Endoscopia/educação , Endoscopia/veterinária , Humanos , Inquéritos e QuestionáriosRESUMO
Canine granulomatous hepatitis is an uncommon morphologic diagnosis that has been associated with a variety of diseases, including a number of systemic infectious etiologies. Formalin-fixed, paraffin-embedded (FFPE) tissues are typically the only source of liver tissue remaining for additional testing for the presence of infectious disease within granulomas. It is unclear if the more common infectious culprits of granulomatous hepatitis can be identified from such specimens. The aim of the current study was to retrospectively investigate archival FFPE liver tissue from dogs with granulomatous hepatitis for the presence of infectious agents. Semiquantitative analysis of copper accumulation in liver specimens was also performed. Medical records were examined for recorded evidence of systemic infectious disease diagnosis. Formalin-fixed, paraffin-embedded liver was prospectively evaluated for infectious agents via differential staining techniques (n = 13), eubacterial fluorescent in situ hybridization (n = 11), and Bartonella polymerase chain reaction assays (n = 15). An infectious cause of granulomatous hepatitis was not identified within liver tissue from any dog using these diagnostic methodologies. Six out of 25 (24%) dogs were diagnosed with concurrent systemic or localized bacterial infections at the time of presentation. Nine out of 17 (53%) dogs had excessive hepatic copper accumulation when evaluated by a semiquantitative histologic grading scheme or quantitative copper analysis. As definitive infectious causes of granulomatous hepatitis were not identified within archival liver biopsy samples, it was concluded that investigation of infectious etiologies within FFPE liver specimens using these diagnostic approaches may be of low yield.
Assuntos
Doenças do Cão/diagnóstico , Granuloma/veterinária , Hepatite Animal/patologia , Fígado/patologia , Preservação de Tecido/veterinária , Animais , Bartonella/isolamento & purificação , Infecções por Bartonella/diagnóstico , Infecções por Bartonella/microbiologia , Infecções por Bartonella/patologia , Infecções por Bartonella/veterinária , Doenças do Cão/patologia , Cães , Feminino , Formaldeído , Granuloma/patologia , Hepatite Animal/diagnóstico , Masculino , Inclusão em Parafina , Estudos RetrospectivosRESUMO
OBJECTIVE: To determine outcome of bougienage for treatment of benign esophageal stricture (BES) in dogs and cats and identify risk factors for the condition. DESIGN: Retrospective case series. ANIMALS: 20 dogs and 8 cats with BES. PROCEDURES: Medical records were reviewed for information on signalment, clinical features, and outcome. Long-term outcome information was obtained with a questionnaire. RESULTS: Esophageal bougienage was performed with dilators ranging from 5 to 15 mm in diameter; median numbers of bougienage procedures were 3 (dogs) and 4.5 (cats). A good outcome, defined as tolerance of solid food with regurgitation less than once a week, was achieved in 14 dogs and 6 cats. Complications were minimal, with nonfatal esophageal perforation occurring in 1 cat. Four dogs and 1 cat were euthanized or died because of esophageal disease. Dogs with BES were more likely to be female, have a recent history of general anesthesia, have received an antimicrobial orally, or have a history of vomiting than were dogs in a reference population. Cats with BES were more likely to have a recent history of general anesthesia, vomiting, or gastrointestinal tract trichobezoars than were cats in a reference population. Doxycycline-induced esophagitis was the suspected cause of BES in 3 cats. Although general anesthesia was associated with development of BES in 18 dogs and 5 cats, concurrent potential causes of esophageal injury were common. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that esophageal bougienage was a safe and effective treatment for most dogs and cats with BES, with outcomes similar to those reported for balloon dilation.
Assuntos
Doenças do Gato/terapia , Dilatação/veterinária , Doenças do Cão/terapia , Estenose Esofágica/veterinária , Animais , Gatos , Dilatação/métodos , Cães , Estenose Esofágica/cirurgia , Esofagoscopia/veterinária , Feminino , Masculino , Estudos Retrospectivos , Fatores de Risco , Resultado do TratamentoRESUMO
CASE DESCRIPTION: A 4-year-old spayed female mixed-breed dog with a history of allergic skin disease was examined because of regurgitation, coughing, and dysphagia that began 15 days after abdominal surgery for correction of gastric dilatation and volvulus. CLINICAL FINDINGS: Severe diffuse esophagitis, esophageal dysmotility, and a benign esophageal stricture at the level of the base of the heart were identified via contrast videofluoroscopy and esophagoscopy. Severe diffuse eosinophilic ulcerative esophagitis was confirmed by histologic examination of esophageal biopsy specimens and cytologic evaluation of specimens obtained by use of a cytology brush. Esophageal eosinophils were evident (14% to 50% of the inflammatory cell population and > 25 eosinophils/hpf). TREATMENT AND OUTCOME: No clinical or endoscopic improvement was evident after treatment with antireflux medications, including a proton-pump inhibitor, following an initial esophageal bougienage procedure. An excellent response characterized by resolution of dysphagia and regurgitation with marked improvement of the esophageal mucosa was evident following intralesional and systemic administration of glucocorticoids, 2 additional esophageal bougienage procedures, and feeding of an elimination diet. CLINICAL RELEVANCE: To our knowledge, the information reported here is the first description of eosinophilic esophagitis (EE) in a dog. Many similarities exist between the condition in the dog reported here and EE in humans. This clinical report highlights the need to consider EE as a differential diagnosis for esophagitis and esophageal strictures in dogs. When appropriate, esophageal biopsy or cytologic specimens should be obtained and examined to investigate the possibility of EE.
Assuntos
Doenças do Cão/patologia , Eosinofilia/veterinária , Esofagite/veterinária , Animais , Anti-Inflamatórios/uso terapêutico , Cães , Eosinofilia/diagnóstico , Eosinofilia/terapia , Esofagite/diagnóstico , Esofagite/patologia , Esofagite/terapia , Feminino , Fármacos Gastrointestinais/uso terapêutico , Prednisona/uso terapêuticoRESUMO
Cattery-housed pedigree cats, located mostly within the USA, have the highest reported prevalence of Tritrichomonas foetus (T foetus) to date. This prospective, multi-institutional, cross sectional study examines the occurrence of T foetus and other enteric parasites in cattery-housed and shelter cats within Australia, where T foetus has only recently been identified. Faecal specimens were collected from 134 cats, including 82 cattery-housed pedigree cats and 52 shelter cats. Faecal examinations performed for most cats included concentration techniques, Snap Giardia test, culture in InPouch medium, and polymerase chain reaction (PCR) amplification of T foetus ribosomal ribonucleic acid (rRNA) genes using species-specific primers. Observed occurrence of T foetus, Giardia species, Isospora species and Toxascaris leonina for cattery-housed cats (and catteries) were 0%, 7.4 (13.8)%, 10.9 (22.6)% and 1.6 (3.2)%, respectively. Observed occurrence of T foetus, Giardia species, Isospora species and hookworms for shelter cats were 0%, 11.5%, 9.8% and 4.9%, respectively. These results suggest the prevalence of T foetus in cattery-housed cats is currently much lower in Australia than in the USA, while Isospora and Giardia species infections are common.
Assuntos
Doenças do Gato/epidemiologia , Doenças do Gato/parasitologia , Infecções Protozoárias em Animais/epidemiologia , Infecções Protozoárias em Animais/parasitologia , Criação de Animais Domésticos/estatística & dados numéricos , Animais , Austrália/epidemiologia , Cruzamento/estatística & dados numéricos , Gatos , Estudos Transversais , Fezes/parasitologia , Feminino , Giardia/isolamento & purificação , Isospora/isolamento & purificação , Masculino , Reação em Cadeia da Polimerase , Estudos Prospectivos , Inquéritos e Questionários , Toxascaris/isolamento & purificação , Tritrichomonas foetus/isolamento & purificaçãoRESUMO
OBJECTIVE: To determine prevalence, clinical features, and causes of epistaxis in dogs. DESIGN: Retrospective case series. ANIMALS: 176 dogs with epistaxis. PROCEDURES: Medical records were reviewed for information related to signalment, clinical features, diagnosis, and outcome. RESULTS: 132 (75%) dogs were initially examined by the hospital's emergency service; prevalence of epistaxis was 0.3%. Dogs with epistaxis were more likely to be old (> or = 6 years), male, and large (> or = 26 kg [58.5 lb]) than were dogs in a reference population. In 109 (62%) dogs with epistaxis, an underlying cause was identified; 115 underlying disorders were identified, with 90 classified as local and 25 classified as systemic. Local causes of epistaxis included nasal neoplasia (n = 35), trauma (33), idiopathic rhinitis (20), and periapical abscess (2). Systemic causes included thrombocytopenia (12), thrombocytopathia (7), coagulopathy (3), hypertension (2), and vasculitis (1). Dogs with local causes were more likely to have unilateral than bilateral epistaxis, but 11 of 21 (52%) dogs with systemic disorders also had unilateral epistaxis. Dogs with systemic disorders were more likely to have clinical signs of systemic disease. Duration of epistaxis (acute vs chronic), severity, and duration of hospitalization were similar for dogs with local versus systemic disorders. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that epistaxis was a common disorder in dogs and frequently regarded as an emergency. Local causes of epistaxis were predominant, but clinical features traditionally thought to be helpful in distinguishing local versus systemic causes could not be reliably used for this purpose.
Assuntos
Doenças do Cão/epidemiologia , Doenças do Cão/etiologia , Epistaxe/veterinária , Doenças Nasais/veterinária , Neoplasias Nasais/veterinária , Fatores Etários , Animais , Intervalos de Confiança , Diagnóstico Diferencial , Doenças do Cão/patologia , Cães , Epistaxe/epidemiologia , Epistaxe/etiologia , Epistaxe/patologia , Feminino , Masculino , Doenças Nasais/epidemiologia , Doenças Nasais/etiologia , Doenças Nasais/patologia , Neoplasias Nasais/epidemiologia , Neoplasias Nasais/etiologia , Neoplasias Nasais/patologia , Razão de Chances , Prevalência , Estudos Retrospectivos , Fatores de Risco , Fatores Sexuais , Resultado do TratamentoRESUMO
OBJECTIVE: To determine whether basal serum or plasma cortisol concentration can be used as a screening test to rule out hypoadrenocorticism in dogs. DESIGN: Retrospective case-control study. ANIMALS: 110 dogs with nonadrenal gland illnesses and 13 dogs with hypoadrenocorticism. PROCEDURES: Sensitivity and specificity of basal serum or plasma cortisol concentrations of either Assuntos
Glândulas Suprarrenais/patologia
, Insuficiência Adrenal/veterinária
, Doenças do Cão/sangue
, Hidrocortisona/sangue
, Insuficiência Adrenal/sangue
, Insuficiência Adrenal/diagnóstico
, Insuficiência Adrenal/genética
, Animais
, Estudos de Casos e Controles
, Diagnóstico Diferencial
, Doenças do Cão/diagnóstico
, Doenças do Cão/genética
, Cães
, Feminino
, Predisposição Genética para Doença
, Masculino
, Nova Escócia/epidemiologia
, Linhagem
, Valor Preditivo dos Testes
, Reprodutibilidade dos Testes
, Estudos Retrospectivos
, Sensibilidade e Especificidade
RESUMO
Gastrointestinal disorders, including chronic diarrhea, are common in canine general practice. Many of these diseases do not have a clearly defined underlying cause, despite thorough diagnostic investigation. This article reviews several syndromes with poorly understood causes that are associated with chronic diarrhea in dogs. Because the immune system plays a central role in the pathogenesis of many of these syndromes, gastrointestinal mucosal immunity is also reviewed. Therapeutic interventions discussed in this article, including diet, immunosuppressive agents, antibiotics, probiotics, and prebiotics, are mostly aimed at modulating the intestinal immune response.