Assuntos
Cesárea/estatística & dados numéricos , Complicações na Gravidez/epidemiologia , Prurido/epidemiologia , Terminologia como Assunto , Estudos de Casos e Controles , Feminino , Humanos , Incidência , Masculino , Gravidez , Gravidez Múltipla/estatística & dados numéricos , Razão de MasculinidadeRESUMO
BACKGROUND: The vulvovaginal gingival syndrome is an uncommon and severe variant of lichen planus characterized by erosions or desquamation of vulval, vaginal, and gingival mucosae with a predilection for scarring and stricture formation. OBJECTIVE: We sought to define the clinical, immunopathologic, and human leukocyte antigen findings in a large cohort of patients. METHODS: The clinical presentation and outcome during long-term follow-up were documented in 40 patients. In addition, human leukocyte antigen typing for class II by polymerase chain reaction and sequence-specific primers was performed. RESULTS: During a mean follow-up period of 8.7 (SD +/- 6.8) years, long-term sequelae included strictures of the esophagus, lachrymal ducts, and external auditory canal; loss of vulval architecture; vaginal stenosis; and buccal mucosal fibrosis. The DQB1( *)0201 allele was present in 80% of patients versus 41.8% of control subjects (P Assuntos
Doenças da Gengiva/imunologia
, Antígenos HLA-DQ/imunologia
, Líquen Plano/imunologia
, Doenças Vaginais/imunologia
, Doenças da Vulva/imunologia
, Adolescente
, Adulto
, Idoso
, Idoso de 80 Anos ou mais
, Alelos
, Feminino
, Doenças da Gengiva/diagnóstico
, Doenças da Gengiva/tratamento farmacológico
, Cadeias beta de HLA-DQ
, Humanos
, Líquen Plano/diagnóstico
, Líquen Plano/tratamento farmacológico
, Pessoa de Meia-Idade
, Índice de Gravidade de Doença
, Síndrome
, Doenças Vaginais/diagnóstico
, Doenças Vaginais/tratamento farmacológico
, Doenças da Vulva/diagnóstico
, Doenças da Vulva/tratamento farmacológico
RESUMO
OBJECTIVES: We sought to evaluate the frequency and clinical characteristics of pruritic dermatoses in pregnancy and to assess a rationalized classification. METHODS: Data of 505 pregnant patients seen at two university-based dermatologic hospitals (1994-2004) were retrospectively studied. RESULTS: Diagnoses included eczema in pregnancy (49.7%), polymorphic eruption of pregnancy (PEP) (21.6%), pemphigoid gestationis (PG) (4.2%), intrahepatic cholestasis of pregnancy (ICP) (3%), prurigo of pregnancy (0.8%), pruritic folliculitis of pregnancy (0.2%), and miscellaneous dermatoses (20.6%). Eczema in pregnancy, prurigo of pregnancy, and pruritic folliculitis of pregnancy showed considerable overlap and were summarized as atopic eruption of pregnancy (AEP). While PEP, PG, and ICP presented in late pregnancy, AEP started significantly earlier. Primigravidae and multiple gestations were characteristic for PEP, abdominal involvement for PEP and PG, and a history of affected pregnancies for ICP. LIMITATIONS: This was a retrospective study. CONCLUSION: We propose classifying the dermatoses of pregnancy as PG, PEP, AEP, and ICP. Stereotypic immunofluorescence and laboratory findings are diagnostic of PG and ICP, whereas distinct clinical characteristics facilitate discrimination between PEP and AEP.
Assuntos
Complicações na Gravidez/classificação , Complicações na Gravidez/diagnóstico , Dermatopatias/classificação , Dermatopatias/diagnóstico , Adolescente , Adulto , Algoritmos , Feminino , Humanos , Pessoa de Meia-Idade , Gravidez , Complicações na Gravidez/epidemiologia , Prurido/classificação , Prurido/diagnóstico , Prurido/epidemiologia , Estudos Retrospectivos , Dermatopatias/epidemiologiaRESUMO
BACKGROUND: Pemphigoid gestationis (PG) is a rare pregnancy-associated subepidermal immunobullous disease that targets hemidesmosomal proteins, particularly BP180. Clinically, PG can resemble the eruption known as polymorphic urticarial papules and plaques of pregnancy (PUPPP), and accurate differentiation between these 2 pruritic pregnancy dermatoses has important implications for fetal and maternal prognoses. Results of epitope mapping studies show that IgG autoantibodies in up to 90% of PG serum samples target the well-defined membrane-proximal NC16a domain of BP180. OBJECTIVE: To examine the usefulness of a commercially available NC16a domain enzyme-linked immunosorbent assay in the serodiagnosis of PG and in the differentiation of PG from PUPPP. PARTICIPANTS: A total of 412 women consisting of pretreatment patients with PG (n = 82), patients with PUPPP (n = 164), and age- and sex-matched controls (n = 166). METHODS: All serum samples were assayed in duplicate. Receiver operating characteristic analyses were performed to determine a cutoff value for the diagnosis of PG and for differentiation from PUPPP and controls. RESULTS: A cutoff value of 10 enzyme-linked immunosorbent assay units was associated with specificity and sensitivity of 96%. CONCLUSIONS: The NC16a enzyme-linked immunosorbent assay is highly sensitive and highly specific in differentiating PG from PUPPP, and it is potentially a valuable tool in the serodiagnosis of PG.
Assuntos
Autoantígenos , Penfigoide Bolhoso/diagnóstico , Complicações na Gravidez/diagnóstico , Urticária/diagnóstico , Adulto , Biópsia por Agulha , Estudos de Casos e Controles , Intervalos de Confiança , Diagnóstico Diferencial , Ensaio de Imunoadsorção Enzimática/métodos , Estudos de Avaliação como Assunto , Feminino , Idade Gestacional , Humanos , Imuno-Histoquímica , Colágenos não Fibrilares , Penfigoide Bolhoso/sangue , Gravidez , Probabilidade , Medição de Risco , Sensibilidade e Especificidade , Testes Sorológicos/métodos , Índice de Gravidade de Doença , Dermatopatias Vesiculobolhosas/diagnóstico , Urticária/sangue , Colágeno Tipo XVIIRESUMO
First described in 1990, paraneoplastic pemphigus is an autoimmune condition that causes considerable morbidity, is resistant to therapy and is frequently fatal. Clinical heterogeneity is being recognized as more cases are reported and the documented auto-antigen profile is also increasing. Target antigens are now known to be not restricted to the skin, suggesting that this condition is part of a paraneoplastic autoimmune multiorgan syndrome.
Assuntos
Pênfigo/diagnóstico , Pênfigo/terapia , Autoanticorpos , Doenças Autoimunes/diagnóstico , Doenças Autoimunes/imunologia , Doenças Autoimunes/patologia , Doenças Autoimunes/terapia , Humanos , Síndromes Paraneoplásicas/diagnóstico , Síndromes Paraneoplásicas/imunologia , Síndromes Paraneoplásicas/patologia , Síndromes Paraneoplásicas/terapia , Pênfigo/imunologia , Pênfigo/patologiaRESUMO
A 47-year-old man presented with two red-brown plaques on his back. The histology was consistent with erythema elevatum diutinum. The case was unusual in the site of presentation and in the paucity of lesions. Investigations were aimed at screening for associations such as infections, malignancies and inflammatory diseases. Dapsone remained an effective treatment.
Assuntos
Eritema/diagnóstico , Vasculite/diagnóstico , Dorso , Diagnóstico Diferencial , Eritema/sangue , Eritema/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/patologia , Vasculite/sangue , Vasculite/patologiaRESUMO
A 36-year-old woman presented with lesions on her scalp, face and trunk. Histopathological examination of these lesions demonstrated facial trichoepithelioma, and scalp cylindroma. A solitary nodule on the trunk had features of cylindroma, spiradenoma and trichoepithelioma, a previously unreported occurrence. Based on the clinical picture, the diagnosis of Brooke-Spiegler syndrome was established. Genetic studies confirmed the diagnosis, demonstrating a splice site mutation, designated 1518+2T>C, on the CYLD1 gene of chromosome 16q12-q13.
Assuntos
Carcinoma Adenoide Cístico/diagnóstico , Síndromes Neoplásicas Hereditárias/diagnóstico , Neoplasias Cutâneas/diagnóstico , Adulto , Carcinoma Adenoide Cístico/patologia , Enzima Desubiquitinante CYLD , Diagnóstico Diferencial , Face , Feminino , Humanos , Mutação , Síndromes Neoplásicas Hereditárias/patologia , Couro Cabeludo , Neoplasias Cutâneas/patologia , Proteínas Supressoras de Tumor/genéticaRESUMO
Lichen sclerosus is a common, acquired chronic inflammatory skin disease of unknown etiology, although circulating autoantibodies to the glycoprotein extracellular matrix protein 1 (ECM1) have been detected in most patients' sera. We have examined the nature of ECM1 epitopes in lichen sclerosus sera, developed an ELISA system for serologic diagnosis, and assessed clinicopathological correlation between ELISA titer and disease. Epitope-mapping studies revealed that lichen sclerosus sera most frequently recognized the distal second tandem repeat domain and carboxyl-terminus of ECM1. We analyzed serum autoantibody reactivity against this immunodominant epitope in 413 individuals (95 subjects with lichen sclerosus, 161 normal control subjects, and 157 subjects with other autoimmune basement membrane or sclerosing diseases). The ELISA assay was highly sensitive; 76 of 95 lichen sclerosus patients (80.0%) exhibited IgG reactivity. It was also highly specific (93.7%) in discriminating between lichen sclerosus and other disease/control sera. Higher anti-ECM1 titers also correlated with more longstanding and refractory disease and cases complicated by squamous cell carcinoma. Furthermore, passive transfer of affinity-purified patient IgG reproduced some histologic and immunopathologic features of lichen sclerosus skin. This new ELISA is valuable for the accurate detection and quantification of anti-ECM1 autoantibodies. Moreover, the values may have clinical significance in patients with lichen sclerosus.
Assuntos
Ensaio de Imunoadsorção Enzimática , Líquen Escleroso e Atrófico/diagnóstico , Animais , Autoanticorpos/imunologia , Autoanticorpos/farmacologia , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/imunologia , Glutationa Transferase/genética , Glutationa Transferase/imunologia , Humanos , Immunoblotting , Líquen Escleroso e Atrófico/imunologia , Camundongos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologiaRESUMO
Autoimmune bullous diseases are often associated with significant morbidity and some can even cause mortality, if left untreated. Multiple therapies are now available to treat these blistering conditions. However, few have been evaluated objectively.
Assuntos
Doenças Autoimunes/terapia , Dermatopatias Vesiculobolhosas/terapia , Anti-Inflamatórios/uso terapêutico , Anticorpos Monoclonais/uso terapêutico , Anticorpos Monoclonais Murinos , Doenças Autoimunes/classificação , Doenças Autoimunes/diagnóstico , Azatioprina/uso terapêutico , Ciclofosfamida/uso terapêutico , Ciclosporina/uso terapêutico , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Imunossupressores/uso terapêutico , Metotrexato/uso terapêutico , Ácido Micofenólico/uso terapêutico , Plasmaferese , Rituximab , Higiene da Pele/métodos , Dermatopatias Vesiculobolhosas/classificação , Dermatopatias Vesiculobolhosas/diagnósticoRESUMO
BACKGROUND: Lichen sclerosus is a common acquired inflammatory disorder of skin and mucous membranes. The aetiology is unknown, although HLA-subtype susceptibility and high rates of other autoimmune disorders suggest that autoantibodies to specific mucocutaneous antigens are involved. The clinicopathological similarities between lichen sclerosus and lipoid proteinosis, which results from mutations in extracellular matrix protein 1 (ECM1), suggest this protein as an autoantigen. METHODS: We analysed serum autoantibody profiles in 171 individuals (86 with lichen sclerosus, 85 healthy controls) by immunoblotting of extracts from normal human skin and lipoid proteinosis skin (lacking ECM1). We generated a full-length glutathione-S-transferase fusion protein for ECM1 to confirm specific immunoreactivity. We affinity-purified serum from patients with lichen sclerosus and did indirect immunofluorescence microscopy on normal skin with or without preabsorption with recombinant ECM1. FINDINGS: By immunoblotting, IgG autoantibodies were found in 20 (67% [95% CI 45-84]) of 30 lichen sclerosus serum samples. The highest titre was 1 in 20. The bands were not detected in ECM1-deficient substrate. These samples, and those from 56 other patients with lichen sclerosus, showed immunoreactivity to the recombinant ECM1 protein (64 of 86 positive; 74% [65-84]). Only six (7% [2-13]) of 85 control serum samples were positive. Affinity-purified IgG from serum of patients with lichen sclerosus labelled skin similarly to a polyclonal antibody to ECM1. The positive staining was blocked by preabsorption with excess recombinant ECM1 protein. INTERPRETATION: These findings provide evidence for a specific humoral immune response to ECM1 in lichen sclerosus and offer insight into disease diagnosis, monitoring, and approaches to treatment.
Assuntos
Autoanticorpos/sangue , Proteínas da Matriz Extracelular/imunologia , Líquen Escleroso e Atrófico/imunologia , Adulto , Estudos de Casos e Controles , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Immunoblotting , Imunoglobulina G/sangueRESUMO
We describe the first reported case of persistent oral and genital lichenoid reactions associated with circulating antibodies to 250- and 215-kd proteins, compatible with desmoplakin I and II, respectively. We discuss the potential role of epitope spreading, leading to the novel development of specific autoantibodies to desmoplakin.
Assuntos
Proteínas do Citoesqueleto/efeitos adversos , Doenças dos Genitais Femininos/patologia , Erupções Liquenoides/induzido quimicamente , Úlceras Orais/patologia , Úlcera/patologia , Idoso , Idoso de 80 Anos ou mais , Autoanticorpos/análise , Biópsia por Agulha , Desmoplaquinas , Quimioterapia Combinada , Feminino , Seguimentos , Doenças dos Genitais Femininos/tratamento farmacológico , Doenças dos Genitais Femininos/imunologia , Humanos , Imuno-Histoquímica , Erupções Liquenoides/tratamento farmacológico , Erupções Liquenoides/patologia , Úlceras Orais/induzido quimicamente , Úlceras Orais/tratamento farmacológico , Úlcera/tratamento farmacológico , Úlcera/imunologiaRESUMO
A gravidez é um estado em que ocorrem profundas mudanças endocrinológicas e metabólicas que säo toleradas pelo organismo por um curto período de tempo. A maioria das dermatoses específicas da gravidez säo de patogenia desconhecida, o que dificulta sua classificaçäo. A terminologia destas dermatoses é confusa, havendo vários nomes para designar condiçöes clínicas semelhantes. Em 1982 Black e Holmes propuseram uma nova classificaçäo mais simplificada e composta por quatro entidades: penfigóide gestacional, erupçäo polimórfica da gravidez, prurigo da gravidez, e foliculite pruriginosa da gravidez. A presente revisäo baseia-se nesta classificaçäo e apresenta cada dermatose separadamente