RESUMO
OBJECTIVES: To evaluate the analytical performance of the Bio-Rad Variant II HbA(1C) analyzer (a completely automated system for the quantification of glycohemoglobin [HbA(1C)] in blood). DESIGN AND METHODS: The analytical parameters of precision, linearity and analytical range were assessed and HbA(1C) results from the Variant II were compared to HbA(1C) results from the Bio-Rad Variant (a method certified by the National Glycohemoglobin Standardization Program). The effect of a variety of hemoglobin variants on HbA(1C) obtained on the system was investigated. RESULTS: Total imprecision was less than 5% and the results compared well with those from an established method. The method has a wide analytical range with no carryover between specimens. CONCLUSION: The HbA(1C) method on the Variant II gives acceptable analytical performance.
Assuntos
Hemoglobinas Glicadas/análise , Automação , Análise Química do Sangue/instrumentação , Técnicas de Química Analítica/métodos , Cromatografia Líquida de Alta Pressão , Diabetes Mellitus/sangue , Hemoglobina Fetal/análise , Liofilização , Variação Genética , Hemoglobina E/análise , Hemoglobina J/análise , Hemoglobinas Anormais/análise , Humanos , Valores de Referência , Análise de Regressão , Reprodutibilidade dos Testes , Estatística como AssuntoAssuntos
Cromatografia Líquida de Alta Pressão , Hemoglobina Fetal/análise , Hemoglobina A2/análise , Homozigoto , Talassemia beta/diagnóstico , Contagem de Células Sanguíneas , China/etnologia , Feminino , Predisposição Genética para Doença , Humanos , Lactente , Talassemia beta/sangue , Talassemia beta/genéticaRESUMO
OBJECTIVES: The objective was to compare hemoglobin A1C (HbA1C) results obtained by two methods based on different analytical principles for individuals with a structural hemoglobin variant. DESIGN AND METHODS: Hemoglobin A1C results were obtained using the Bio-Rad Variant (based on cation exchange chromatography) and the Bayer DCA 2000 (based on an immunological reaction) on individuals with a structural hemoglobin variant. The identity of the hemoglobin variant was confirmed by high pressure liquid chromatography (HPLC) and electrophoresis. RESULTS: Hemoglobin A1C results obtained by the two methods on individuals with S, C, D, and E trait were in close agreement. CONCLUSION: The Bio-Rad Variant and Bayer DCA 2000 produce equivalent hemoglobin A1C results on patients with S, C, and E trait. With appropriate correction, correlation of hemoglobin A1C results from the Bio-Rad Variant for individuals with D trait was good (r = 0.927). Glycohemoglobin results obtained by the two methods for some unusual structural hemoglobin variants were in close agreement.
Assuntos
Cromatografia por Troca Iônica/métodos , Hemoglobinas Glicadas/análise , Hemoglobinas Anormais/análise , Testes Imunológicos/métodos , Hemoglobina C/análise , Hemoglobina E/análise , Hemoglobina Falciforme/análise , HumanosRESUMO
1. A spectrophotometric scanning technique was used to quantitate hemoglobin concentrations in cardiac surgery patients and patients with other hemolytic disorders. 2. The fractional absorption of a portion of the 577 nm band of oxyhemoglobin was used in conjunction was used in conjunction with the absorption coefficient of oxyhemoglobin to relate absorption to plasma hemoblogin concentration. 3. The spectrophotometric scanning technique offers a rapid and accurate means of assessing plasma hemoglobin concentrations even in the presence of usual interferences by bilirubin, turbidity and methemalbumin.