Positive relationship between soil fertility, plant diversity, and gall richnes

The complexity of nutrient distribution patterns in soils is a determinant environmental component of the structure of plant communities. Numerous insect species that interact with plants are associated with these communities, and some of these interactions result in the formation of unusual structures called galls. In this study, we investigated the relationship of galls, soil fertility and plant communities in three vegetation types, herbaceous restinga (HR), shrub restinga (SR) and shrub-tree restinga (STR), in an area of restinga in southern Brazil. We identified 217 species belonging to 159 genera and 82 families. The plant diversity recorded in the STR was 42.8% higher than the diversity in the other vegetation types. Gall richness increased significantly with increased plant richness. The edaphic gradient was correlated with the floristic diversity in the vegetation types. Our data suggest that an increment in soil fertility (organic matter and litter thickness), associated with climatic conditions, should increase the number of plants that can potentially host galls and, consequently, the richness of galling insects. Gall richness may also be influenced by a higher occurrence of woody plants, due to an increase in leaf surface area available in the tree canopy, especially in STR.

Positive relationship between soil fertility, plant diversity, and gall richnes

The complexity of nutrient distribution patterns in soils is a determinant environmental component of the structure of plant communities. Numerous insect species that interact with plants are associated with these communities, and some of these interactions result in the formation of unusual structures called galls. In this study, we investigated the relationship of galls, soil fertility and plant communities in three vegetation types, herbaceous restinga (HR), shrub restinga (SR) and shrub-tree restinga (STR), in an area of restinga in southern Brazil. We identified 217 species belonging to 159 genera and 82 families. The plant diversity recorded in the STR was 42.8% higher than the diversity in the other vegetation types. Gall richness increased significantly with increased plant richness. The edaphic gradient was correlated with the floristic diversity in the vegetation types. Our data suggest that an increment in soil fertility (organic matter and litter thickness), associated with climatic conditions, should increase the number of plants that can potentially host galls and, consequently, the richness of galling insects. Gall richness may also be influenced by a higher occurrence of woody plants, due to an increase in leaf surface area available in the tree canopy, especially in STR.(AU)

Leaf architecture and symmetry of understory tree species of anAraucaria forest / Arquitetura foliar e simetria de espécies do sub-bosque da floresta com Araucária

Shade plants of ombrophilous forests are subjected to light-limiting conditions and need toinvest in architectural structures associated with leaf symmetry to increase light capture. This study investigated the leaf architecture of six Araucaria forest tree species with distinct symmetry: Cupania vernalis, Casearia sylvestris, Schinus terebinthifolius, Piper gaudichaudianum, Roupala brasiliensis and Cedrela fissilis. We hypothesized that symmetry, associated with other traits, minimizes self-shading. Asymmetry index, petiole length, total leaf area, leaf angle, internode length and stem diameter were measured. The asymmetry index did not indicate a clear distinction between asymmetric and symmetric leaves. Leaves classified as asymmetric had higher values for the asymmetry index in the median and basal regions of the leaf, while symmetrical leaves had higher values in the apical region. The results also indicated an adjustment among structural leaf traits that facilitated a three-dimensional organization that produced an advantageous arrangement for light capture, which seems to be a response to selective pressure by the heterogeneous light conditions of the ombrophilous forest understory.

Nas florestas ombrófilas, as plantas de sombra estão sujeitas a condições limitantes de luz enecessitam investir em atributos estruturais associados à simetria foliar para maximizar a captura da luz. Esse estudo investigou a arquitetura foliar de seis espécies arbóreas (Cupania vernalis, Casearia sylvestris, Schinus terebinthifolius, Piper gaudichaudianum, Roupala brasiliensis e Cedrela fissilis) da Floresta com Araucária. Nossa hipótese é que a simetria foliar, associada a outros atributos foliares, minimiza o autosombreamento. Foram mensurados o índice de assimetria, o comprimento do pecíolo, a área foliar total, ângulo foliar, o comprimento do internó e o diâmetro do caule. O índice de assimetria não indicou uma distinção clara entre folhas assimétricas e simétricas. Folhas classificadas como assimétricas apresentaram maior índice na região mediana e basal da folha, enquanto as folhas simétricas apresentaram maior índice na região apical. Os resultados também indicaram um ajuste entre os atributos estruturais que permitiram um arranjo tridimensional das folhas vantajoso para a captura de luz, o que parece ser uma resposta à pressão seletiva pelas condições heterogêneas de luz do sub-bosque de floresta ombrófila.

Leaf architecture and symmetry of understory tree species of anAraucaria forest / Arquitetura foliar e simetria de espécies do sub-bosque da floresta com Araucária

Shade plants of ombrophilous forests are subjected to light-limiting conditions and need toinvest in architectural structures associated with leaf symmetry to increase light capture. This study investigated the leaf architecture of six Araucaria forest tree species with distinct symmetry: Cupania vernalis, Casearia sylvestris, Schinus terebinthifolius, Piper gaudichaudianum, Roupala brasiliensis and Cedrela fissilis. We hypothesized that symmetry, associated with other traits, minimizes self-shading. Asymmetry index, petiole length, total leaf area, leaf angle, internode length and stem diameter were measured. The asymmetry index did not indicate a clear distinction between asymmetric and symmetric leaves. Leaves classified as asymmetric had higher values for the asymmetry index in the median and basal regions of the leaf, while symmetrical leaves had higher values in the apical region. The results also indicated an adjustment among structural leaf traits that facilitated a three-dimensional organization that produced an advantageous arrangement for light capture, which seems to be a response to selective pressure by the heterogeneous light conditions of the ombrophilous forest understory.(AU)

Nas florestas ombrófilas, as plantas de sombra estão sujeitas a condições limitantes de luz enecessitam investir em atributos estruturais associados à simetria foliar para maximizar a captura da luz. Esse estudo investigou a arquitetura foliar de seis espécies arbóreas (Cupania vernalis, Casearia sylvestris, Schinus terebinthifolius, Piper gaudichaudianum, Roupala brasiliensis e Cedrela fissilis) da Floresta com Araucária. Nossa hipótese é que a simetria foliar, associada a outros atributos foliares, minimiza o autosombreamento. Foram mensurados o índice de assimetria, o comprimento do pecíolo, a área foliar total, ângulo foliar, o comprimento do internó e o diâmetro do caule. O índice de assimetria não indicou uma distinção clara entre folhas assimétricas e simétricas. Folhas classificadas como assimétricas apresentaram maior índice na região mediana e basal da folha, enquanto as folhas simétricas apresentaram maior índice na região apical. Os resultados também indicaram um ajuste entre os atributos estruturais que permitiram um arranjo tridimensional das folhas vantajoso para a captura de luz, o que parece ser uma resposta à pressão seletiva pelas condições heterogêneas de luz do sub-bosque de floresta ombrófila.(AU)

Distribution of Cu, Fe, Mn, and Zn in Two Mangroves of Southern Brazil

ABSTRACT This study assessed the relation between Cu, Fe, Mn, and Zn in the soils of the mangroves of Antonina and Guaratuba, on the coastline of the State of Paraná, and in the leaf tissues of A. shauerianaR. mangle, and L. racemosa through the analysis of correlation levels between these two compartments. Leaf samples were collected for ten individuals of each species in a 1000 m2 area delimited in each mangrove. Soil samples from 0-10 cm depth were taken from under the crown projection area of the selected trees to be submitted to chemical analysis. In the soils, metallic micronutrients presented the following order: Fe > Mn > Zn > Cu. In the leaves, concentrations were species dependent. InA. shaueriana and R. mangle, the profile was Mn > Fe > Zn > Cu, while in L. racemosa, the sequence was: Fe > Mn > Zn > Cu. Correlation analyses revealed only four significant correlations for Mn, Zn, and Cu in the soil and plant compartments. These results suggested that significant correlations depended on abiotic factors, inhibition between the elements, and immobilization and/or adsorptions of these metals by the soil.

Salt stress alters the cell wall polysaccharides and anatomy of coffee (Coffea arabica L.) leaf cells.

Coffea arabica is the most important agricultural commodity in the world, and salinity is a major threat to its sustainable irrigation. Coffee leaf polysaccharides from plants subjected to salt stress were extracted and the leaves visualized through optical and electron microscopy. Alterations were detected in the monosaccharide composition of the pectin and hemicelluloses, with increases in uronic acid in all fractions. Changes in the polysaccharides were confirmed by HPSEC and FTIR. Moreover, the monolignol content was increased in the final residue, which suggests increased lignin content. The cytoplasm was altered, and the chloroplasts appeared irregular in shape. The arrangement of the stroma lamellae was disordered, and no starch granules were present. It was concluded that leaves of C. arabica under salt stress showed alterations in cell wall polysaccharides, increased monolignol content and structural damage to the cells of the mesophyll.

Leaf Morphological Plasticity of Tree Species from Two Developmental Stages in Araucaria Forest

This study compared the morphological and anatomical variations of the leaves of four shade-tolerant tree species Allophylus edulis (St.-Hil.) Radlk (Sapindaceae), Casearia sylvestris Sw. (Salicaceae), Cupania vernalis Cambess. (Sapindaceae) and Luehea divaricata Mart. (Malvaceae) from a fragment of Araucaria forest in two developmental stages. Morphological and anatomical traits, such as leaf and tissue thickness, leaf area, leaf dry mass, specific leaf area, leaf density and stomata density were measured from 30 leaves of each developmental stage. The phenotypic plasticity index was also calculated for each quantitative trait. The results showed that the four species presented higher mean values ​​for specific leaf area and spongy/palisade parenchyma ratio at young stage, and higher mean values ​​for stomata density, total and palisade parenchyma thickness in the adult stage. The plasticity index demonstrated that L. divricata presented highest plasticity for both the morphological and anatomical traits while A. edulis displayed the lowest plasticity index. The results of this study indicated that the leaves of these species exhibited distinct morphological traits at each stage of development to cope with acting environmental factors.

Heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (Coffea arabica L.).

Coffee plants were subjected to heat stress (37 °C) and compared with control plants (24 °C). Cell wall polysaccharides were extracted using water (W), EDTA (E) and 4M NaOH (H30 and H70). In addition, monolignols were analyzed, and the leaves were observed by microscopy. Plants under heat stress accumulated higher contents of arabinose and galactose in fraction W. Xylose contents were observed to decrease in H30 fractions after the heat stress, whereas galactose and uronic acid increased. H70 fractions from plants exposed to heat stress showed increased xylose contents, whereas the contents of arabinose and glucose decreased. Differences in the molar-mass profiles of polysaccharides were also observed. The primary monolignol contents increased after the heat stress. Structural alterations in palisade cells and ultrastructural damage in chloroplasts were also observed. Our results demonstrate that the chemical profile of coffee cell-wall polymers and structural cell anatomy change under heat stress.

Testing hypotheses for morphological differences among populations of Miconia sellowiana (Melastomataceae) in southern Brazil / Testando hipóteses para as diferenças morfológicas entre populações de Miconia sellowiana (Melastomataceae) do sul do Brasil

Previous studies have uncovered considerable variability in foliar morphology and anatomy for Miconia sellowiana in different types of vegetation (Grassland, Montane Atlantic forest, Upper Montane Atlantic forest and Araucaria Pine forest). Although such variability could be due to phenotypic plasticity, an alternative explanation for this phenomenon is the existence of genetic differentiation among populations resulting from genetic drift or adaptation to different environments. The goal of the present study was to investigate the extent of genetic structures among populations of Miconia sellowiana using a neutral dominant genetic marker (RAPD - Random Amplification of Polymorphic DNA). There was considerable variability in the studied samples, resulting in 96.5% polymorphic loci and a Gst = 0.13. The analysis of molecular variance showed the populations are genetically structured (p < 0.001). The subpopulations of M. sellowiana were grouped similarly together using genetic (based on a neutral marker) or morphological dendrograms, suggesting that the morphological differences observed are the result of local genetic differentiation by genetic drift and not the alleged phenotypic plasticity of the species.

Estudos prévios relatam a variabilidade na morfologia e anatomia de Miconia sellowiana em diferentes formações vegetacionais (Estepe Gramínio-Lenhosa, Floresta Ombrófila Densa Montana, Floresta Ombrófila Densa Alto-Montana e Floresta Ombrófila Mista). Apesar dessa variabilidade poder ser devido à plasticidade fenotípica, uma explicação alternativa para o mesmo fenômeno é a existência de diferenciação genética entre as populações, resultado de deriva genética ou adaptação aos diferentes ambientes. O objetivo do presente estudo foi investigar a existência de estruturação genética entre as populações de M. sellowiana, utilizando um marcador genético dominante e neutro (RAPD - "Random Amplification of Polymorphic DNA"). Foi encontrado um grau considerável de variabilidade nas amostras estudadas, sendo que 96,5% dos locos foram polimórficos e o valor de Gst foi de 0,13. O número estimado de migrantes por geração foi de 3,19, o que consiste com a existência de um fluxo gênico reduzido entre os locais estudados. Esse resultado foi confirmado pela análise de variância molecular (p < 0,001). As subpopulações de M. sellowiana ficaram igualmente agrupadas nos dendrogramas dos dados genéticos (baseado no marcador molecular neutro) e morfológicos, sugerindo que as diferenças morfológicas encontradas são resultado da diferenciação genética local e não por plasticidade fenotípica da espécie.

Morpho-anatomical and micromorphometrical evaluations in soybean genotypes during water stress

In a greenhouse experiment, morpho-anatomical and micromorphometrical analyses of two soybean cultivars, MG/BR46 (Conquista) and BR16-tolerant and sensitive to drought, respectively-were used to study their water-deficit-tolerance strategies. Drought treatments were applied at reproductive stages from R2 to R7, where evaluations were conducted at 30 days and 45 days after stress started, respectively. The total length of Conquista plants (shoot + root) was greater than of BR16 plants. Pod dry weight was adversely affected due to the lack of moisture, decreasing productivity even of Conquista plants. Both the cultivars had normal development of root hairs; however, it was observed a decrease in the cortex:central cylinder ratio in BR16 stressed for 30 days, and they also showed similar leaflet thickness and stomata distribution. Differences in drought tolerance observed between the two cultivars seemed to be related to factors other than morphological traits since this species has a short lifecycle.

Análises morfo-anatômicas e micromorfométricas de duas cultivares de soja, MG/BR46 (Conquista) e BR16—tolerante e sensível à seca, respectivamente, em experimento conduzido em casa de vegetação—foram feitas para estudar as diferentes estratégias de tolerância ao déficit hídrico. Tratamentos de seca foram aplicados no estádio reprodutivo R2 e R7, onde avaliações foram conduzidas em 30 dias e 45 dias após o inicio do estresse, respectivamente. O comprimento total das plantas da cultivar Conquista (parte aérea e raiz) foi maior do que das plantas da cultivar BR16. A massa seca da vagem foi adversamente afetada pelo déficit hídrico, diminuindo a produtividade das plantas da cultivar Conquista. Ambas as cultivares tiveram o desenvolvimento de pêlos radiciais normais e, uma diminuição da razão córtex:cilindro central foi observada em BR16 estressada por 30 dias, além de apresentar uma espessura do folíolo e distribuição dos estômatos normais. Diferenças na tolerância à seca observada entre as duas cultivares devem estar relacionadas também a outros fatores, alm das características morfológicas, já que esta espécie possui um ciclo de vida curto.

Physiological aspects of sun and shade leaves of Lithraea molleoides (Vell.) Engl. (Anacardiaceae)

The aim of this work was to compare the physiological parameters of sun and shade leaves of a specimen of L. molleoides. The higher-positional leaves, classified as sun leaves, presented similar photosynthetic rate, lower chlorophyill contents (a, b and total), same a chlorophyll /b chlrorophyll rate, lower transpiratory rate, same stomatal conductance and intercellular concentration of CO2 as the lower-positional leaves, classified as shade leaves. Nutrient concentration, except for Ca and Mg, was the same for both sun and shade leaves.The physiological parameter responses indicated that although receiving lower light intensity, the shade leaves had the same capacity to grow and develop as the sun leaves.

O presente trabalho teve como objetivo avaliar de forma comparativa alguns parâmetros fisiológicos de folhas de sol e de sombra de um espécime de L. molleoides. Esta espécie é conhecida popularmente como aroeira-branca. As folhas da porção superior periférica, denominadas folhas de sol, apresentaram igual taxa fotossintética, menores teores de clorofilas (a, b e totais), mesma razão clorofila a/ clorofila b, menor taxa transpiratória, mesma condutância estomática e concentração intercelular de CO2 em relação às folhas da porção inferior interna, denominadas folhas de sombra. A concentração de nutrientes, excetuando Ca e Mg, foi igual em folhas de sol e de sombra. As respostas dos parâmetros fisiológicos avaliados indicam que as folhas de sombra, apesar de receberem menor intensidade luminosa, têm possibilidade de crescer e se desenvolver com a mesma capacidade das folhas de sol.

Leaf morphology of 89 tree species from a lowland tropical rain forest (Atlantic forest) in South Brazil

Este estudo examinou a morfologia e anatomia foliar de 89 espécies arbóreas de uma área de Floresta Atlântica Litorânea no Sul do Brasil. A maioria das espécies (>75%) possuem folhas pequenas (notófilas e micrófilas) elípticas e simples com margens inteiras. Essas folhas apresentam uma estrutura anatômica formada por epiderme uniseriada, parênquima paliçádico composto por um único estrato de células e o parênquima esponjoso composto por 5 to 8 estratos celulares. O tecido esclerenquimático é limitado aos feixes vasculares. A maioria das espécies arbóreas (91%) possui folhas com características mesomórficas Poucas espécies (9%) são xerófilas como era esperado em florestas que crescem sob solo arenoso oligotrófico. As características mesomórficas parecem estar associadas a eficientes mecanismos de ciclagem de nutrientes que compensam o baixo conteúdo de nutrientes do solo mineral.