RESUMO
The fauna of bloodsucking mosquitoes in the Nizhny Novgorod Region is represented by 11 species from 5 genera of the family Culicidae. During 2014-2015, the predominant species were Ochlerotatus cantans and Aedes cinereus mosqui- toes in both a population aggregate and woodland. The infected mosquitoes accounted for 1.3% of their total number and were registered only in the village of Fokino. The investigators identified two human pathogenic nematode species: Diro- filaria immits and Dirofilaria repens (0.9% and 0.4% respectively). The effective carriers of Dirofilaria in the examined area can be Ae.cinereus and Och.cantans as only these species were found to have an invasive stage of the parasite. The symbiotic bacterium Wolbachia was detected in the mosquitoes that were not infected with dirofilariasis. This is the first study in Russia to investigate the effects of Wolbachia on the susceptibility of dirofilariasis vectors to infection.
Assuntos
Dirofilariose/microbiologia , Dirofilariose/parasitologia , Mosquitos Vetores/microbiologia , Mosquitos Vetores/parasitologia , Simbiose , Aedes/microbiologia , Aedes/parasitologia , Animais , DNA de Helmintos/genética , Dirofilaria immitis/microbiologia , Dirofilaria immitis/patogenicidade , Dirofilaria repens/microbiologia , Dirofilaria repens/patogenicidade , Cães , Humanos , Mosquitos Vetores/genética , Wolbachia/isolamento & purificação , Wolbachia/patogenicidadeRESUMO
Blood-sucking mosquitoes (n = 2277) collected in Tula and its Region in 2013-2014 were examined using a PCR assay for dirofilariae. A total of 12 species from 4 genera (Culiseta, Aedes, Ochlerotatus [foreign character] Culex) out of 18 found mosquito species were infected with Dirofilaria immitis and D. repens. The proportion of the infected mosquitoes was 2.5% (D. immitis, 1.5%; D.repens, 1%). According to preliminary data, the most efficient Dirofilaria vectors, in the Tula Region may be Ae. vexans, Ae. geniculatus, Och. cantans, and Cx. pipiens.
Assuntos
Culicidae/parasitologia , Dirofilaria immitis/crescimento & desenvolvimento , Dirofilaria repens/crescimento & desenvolvimento , Interações Hospedeiro-Parasita , Insetos Vetores/parasitologia , Estágios do Ciclo de Vida/fisiologia , Animais , Culicidae/classificação , DNA de Helmintos/genética , Dirofilaria immitis/genética , Dirofilaria immitis/patogenicidade , Dirofilaria repens/genética , Dirofilaria repens/patogenicidade , Dirofilariose/epidemiologia , Dirofilariose/parasitologia , Dirofilariose/patologia , Dirofilariose/transmissão , Cães , Monitoramento Epidemiológico , Feminino , Humanos , Masculino , Reação em Cadeia da Polimerase , Federação Russa/epidemiologiaRESUMO
Bloodsucking mosquitoes were collected in Tula and its Region in May to August 2013-2014. The fauna included 17 species from 5 genera in the subfamily Culicinae and Anopheles maculipennis complex in the subsystem Anophelinae. Ochlerotatus cantans was a dominant species in the collections. The dominant species also included Aedes einereus, Ae. vexans, Ae. geniculatus, Och. diantaeus, Och. intrudens, Och. Cataphylla, and Culex pipiens. The possible value of different mosquito species Dirofilaria repens and D. immitis as vectors of dirofilarasis was discussed.
Assuntos
Anopheles/classificação , Dirofilaria/classificação , Dirofilariose/transmissão , Animais , Anopheles/parasitologia , Dirofilaria/parasitologia , Dirofilariose/parasitologia , Ecologia , Humanos , Insetos Vetores/classificação , Filogeografia , Federação RussaRESUMO
Infusoria Tetrahymena pyriformis are effective as test objects to evaluate endotoxicity degree in acute occupational intoxications with carbon monoxide. Biologic test (for Tetrahymena pyriformis) and biochemical markers of endogenic intoxication appear to have strong correlation.
