RESUMO
Production of Enterobifidin comprises preparation of culture media, reparation of lyophilized Bifidobacterium adolescentis MS-42 culture, preparation of starters, cultivation of bacteria in fermenters, biomass conservation, and its biological control. The preparation contains physiologically active bifidobacterium cells with high activities of growth (mu = 0.7 h-1, g = 1.0 h) and acid formation (titratable acidity is approximately 120-140 degrees T; acetate concentration, 0.50-0.75%; and lactate concentration, 0.33-0.50%). The antagonistic activity of these bacteria towards Escherichia coli 08, E. coli 086, E. coli 015, E. coli 0115, and E. coli 0101 amounts to 98.2;, to Proteus vulgaris 102, to 87.2; and Staphylococcus aureus 209p, to 83.2%. The bifidobacteria (with a titer of 10(9) CFU/ml) remained viable for two to five months.
Assuntos
Antibacterianos/metabolismo , Bifidobacterium/metabolismo , Produtos Biológicos/biossíntese , Bifidobacterium/crescimento & desenvolvimento , CinéticaRESUMO
Morphologic heterogeneity of cells from developing populations of bifidobacteria correlates with ultrastructure peculiarities. Active proliferating cells in exponential phase are characterized by formation of intracytoplasmatic membrane complex represented by lamellar, myelinoform, vesicular structures. Nucleoid is localized as the central polybranched or disperse osmophobic zone. Nucleoid distribution is determined by morphogenesis processes--exobudding, branching or multiseptation. Electronograms reveal multiple polyphosphate and polysaccharide inclusions. Ageing of bifidobacterial populations is accompanied with ultrastructural changes: cell wall hypersynthesis, reorganization and increased size of intracytoplasmatic membrane complex, altered morphology and compactness of nuclei, formation and dissimilation of inclusions.
