RESUMO
The research included evaluation of express-diagnosis capability of immunochromatographic assay (ICA) Binax NOW (Alere, Inc., USA) for diagnosis of the rhinosinusitis caused by to detect the Streptococcus pneumoniae antigen directly in clinical samples. The unique feature of the method included obtaining samples with an electric suction machine in order to evaluate aspirate from deep parts of the nasal cavity. Diagnostic capability of the Binax NOW was determined in a comparative study using classical bacteriological method in 100 clinical samples. Pneumococcus was isolated in 16 patients (16±3,7%) via bacteriological method. ICA utilization allowed to reveal pneumococcal antigen in 20 cases (20±4,0%). ICA test sensitivity equaled 87,5%, specificity - 92,9%. Obtained results allow us to recommend ICA for identification of pneumococcal infection in patients with sinusitis for practicing physicians. The advantages of the evaluated method were fast results (for up to 15 min) and possibility of non-invasive sampling technique of clinical specimens.
Assuntos
Infecções Pneumocócicas , Rinite , Sinusite , Antígenos de Bactérias , Humanos , Infecções Pneumocócicas/diagnóstico , Rinite/diagnóstico , Sensibilidade e Especificidade , Sinusite/diagnóstico , Streptococcus pneumoniaeRESUMO
AIM: Determine features of epidemic process (EP) of Sonnei dysentery in Khabarovsk Region in 2012 - 2014 due to atypical causative agent. MATERIALS AND METHODS: Detailed characteristics of 161 cultures of Shigella sonnei isolated from 81 patients from epidemic focus in children boarding school in Bikin as well as from 22 patients from sporadic and group foci of dysenteryin Khabarovsk (biochemical type, colicin-genotype, spectrum of drug resistance) is given. Molecular-biologic subtyping was carried out for 11 strains by Pulsed Field Gel Electrophoresis method (PFGE). RESULTS: Materials of observation of a prolonged foci of Sonnei dysentery with contact-domestic transmission route of the infection in children boarding house for disabled (October 2012 - September 2014) are presented. The diseases are etiologically connected with atypical mannitol- negative types of shigella isolated for the first time in 40 years of observation in Khabarovsk region. Epidemic process of shigellosis was supported by prolonged carriership of the causative agent in patients and special contingent ofthe nursing home. Shigella cultures isolated in the focus belonged to the same colicin-genotype and 2 distinct drug resistance clones, but a single genotype established by PFGE method. CONCLUSION: Results of the studies give evidence on the importance of determi- nation of traditional phenotypic and contemporary genotypic variants of shigella and the neces- sity of search for arguments, additional methodic approaches for establishing similarities and differences of shigella isolates from within the same outbreak of the diseases as well as for com- parison of strains circulating in different territories.
Assuntos
Técnicas de Tipagem Bacteriana , Surtos de Doenças , Farmacorresistência Bacteriana , Disenteria Bacilar , Genótipo , Shigella sonnei , Disenteria Bacilar/epidemiologia , Disenteria Bacilar/microbiologia , Feminino , Humanos , Masculino , Manitol/metabolismo , Shigella sonnei/classificação , Shigella sonnei/genética , Shigella sonnei/isolamento & purificação , Shigella sonnei/metabolismo , Sibéria/epidemiologiaRESUMO
On the basis of their experiences with the treatment of 1306 children the authors discuss problems of diagnostics and treatment of different congenital and acquired diseases of the lungs and esophagus.
Assuntos
Doenças Torácicas/cirurgia , Adolescente , Brônquios/anormalidades , Brônquios/cirurgia , Criança , Pré-Escolar , Humanos , Pulmão/anormalidades , Pulmão/cirurgia , Traumatismos Torácicos/cirurgia , UcrâniaRESUMO
On the basis of an experience with 24 reoperations on the lungs in children at late terms, indications have been developed for such operations and for their volume. A rational complex of treatment in the period of preoperative management was determined. Technical and tactical errors occupied the main place in the structure of causes resulting in reoperations. Recovery was achieved in all the 24 patients.
Assuntos
Pneumopatias/cirurgia , Complicações Pós-Operatórias , Atelectasia Pulmonar/etiologia , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Masculino , ReoperaçãoRESUMO
The experience of treatment of 253 cases of benign tumors and cysts of the mediastinum is summarized. Complex X-ray and endoscopic examination was used for diagnosis. Due to application of thoracoscopy, topical and morphologic diagnosis was improved. Surgery was the main method of treatment. Radical operations were carried out in 98.9% and in 6.3% they involved resection of the rib, lung, pericardium, the membranous part of the trachea and bronchus, esophageal diverticulum or removal of diaphragmatic hernia. The complicated and life-threatening nature of the procedure is mirrored in the postoperative lethality rate (1.57%) and pattern of complications and requires a strict observance of the suggested principles of surgery.
Assuntos
Cisto Mediastínico/diagnóstico , Neoplasias do Mediastino/diagnóstico , Diagnóstico Diferencial , Humanos , Cisto Mediastínico/cirurgia , Neoplasias do Mediastino/cirurgia , ToracoscopiaRESUMO
The pathological process due to Coxsackie A18 virus and hemolytic streptococcus infection was studied in adult white mice. Their synergistic effect was established as manifested by longer periods of virus detection in the blood and heart and the detection of the streptococcus in animals infected with an 8-fold lower dose. In cases of combined infection, signs of dystrophy, destruction and proliferation developed in the myocardium reaching the highest intensity when the virus and the microbe had been inoculated simultaneously.
