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Resumo Introdução: A obesidade vem crescendo em crianças e adolescentes e isso já se tornou uma preocupação de saúde pública. Objetivo: O objetivo do estudo foi comparar e verificar a concordância entre 3 métodos indiretos para estimar a gordura corporal em crianças e adolescentes. Materiais e métodos: Participaram da amostra 90 crianças de ambos os gêneros, sendo 33 (meninos) e 57 (meninas), com uma idade média de 9.45±0.72 anos. Os métodos utilizados para avaliar a gordura corporal foram: 1) Bioimpedância Tetrapolar (BIA); Dobras Cutâneas (DC) e; Índice de Adiposidade Corporal (IAC). Na análise estatística foi utilizado o teste de Shapiro Wilk para verificar a distribuição da amostra. Para comparar os métodos entre os grupos foi adotado o teste Anova de Medidas Repetidas com a utilização do Post Hock de Bonferroni. Para verificar a concordância entre os métodos nas variáveis do estudo foi utilizada análise visual de Bland-Altman. O valor de p foi de p<0.05. Resultados: Os resultados apontam que existiu concordância e correlação positiva entre os métodos estudados. Conclusão: Os métodos apresentaram confiabilidade na análise realizada, no entanto a BIA subestimou a gordura corporal, quando comparada aos demais métodos.
Abstract Introduction: The incidence of obesity has increased in children and adolescents, and this has become an obvious concern for public health. Objective: The aim of this study is to compare and verify the consistency of three indirect methods used for estimating body fat in children and adolescents. Materials and methods: Sample consisting of 90 children of both genders -33 (males) and 57 (females)-, and mean age of 9.45±0.72 years. To estimate body fat, three doubly indirect methods were applied: 1) bioelectrical impedance analysis method (BIA); 2) Skinfold methods (SM), and 3) body adiposity index method (BAI). Shapiro Wilk test was used for statistical analysis in order to verify sample distribution. The repeated measures ANOVA test with Bonferroni's Post Hock was used for comparing the methods among the groups. The Bland-Altman plot and analysis was used to verify the consistency between the methods regarding the studied variables. Results: The results suggest that there was consistency and a positive correlation between the methods studied. Conclusion: The methods were reliable regarding the performed analysis; however, BIA underestimated body fat, when compared to other methods.
RESUMO
BACKGROUND: We have recently shown that the acute infusion of angiotensin-(1-7) [Ang-(1-7)] or chronic increase in plasma Ang-(1-7) produces important changes in regional blood flow in rats. METHODS: To further assess whether these changes are related to Mas, in this study hemodynamic measurements were performed in Ang-(1-7) receptor Mas knockout C57BL/6 (Mas-KO) mice and age-matched wild type (WT) control mice, using fluorescent microspheres. RESULTS: Mean arterial pressure in urethane-anesthetized Mas-KO mice (12-16 weeks old) did not differ from that in WT mice (79 ± 2 and 80 ± 2 mmHg respectively). However, pronounced differences were observed in other hemodynamic measurements. Mas-KO mice exhibited a significant decrease in stroke volume (0.03 ± 0.01 versus 0.05 ± 0.01 ml/beat in WT) and decreased cardiac index (0.81 ± 0.08 versus 1.24 ± 0.24 ml/min/g in WT). Strikingly, Mas-KO mice exhibited a marked increase in vascular resistance and a decrease in blood flow in the kidney, lung, adrenal gland, mesentery, spleen and brown fat tissue. The decrease in blood flow ranged from 34% (spleen) to 55% (brown fat tissue). CONCLUSION: These results suggest that the Ang-(1-7)/Mas axis plays an important role in regional and systemic hemodynamic adjustments in mice.