Cold and Hungry: Heterothermy Is Associated with Low Leptin Levels in a Bulk Grazer during a Drought.

AbstractReduced energy intake can compromise the ability of a mammal to maintain body temperature within a narrow 24-h range, leading to heterothermy. To investigate the main drivers of heterothermy in a bulk grazer, we compared abdominal temperature, body mass, body condition index, and serum leptin levels in 11 subadult Cape buffalo (Syncerus caffer caffer) during a drought year and a nondrought year. Low food availability during the drought year (as indexed by grass biomass, satellite imagery of vegetation greenness, and fecal chlorophyll) resulted in lower body condition index, lower body mass relative to that expected for an equivalent-aged buffalo, and lower leptin levels. The range of 24-h body temperature rhythm was 2°C during the nondrought year and more than double that during the drought year, and this was caused primarily by a lower minimum 24-h body temperature rhythm during the cool dry winter months. After rain fell and vegetation greenness increased, the minimum 24-h body temperature rhythm increased, and the range of 24-h body temperature rhythm was smaller than 2°C. In order of importance, poor body condition, low minimum 24-h air temperature, and low serum leptin levels were the best predictors of the increase in the range of 24-h body temperature rhythm. While the thermoregulatory role of leptin is not fully understood, the association between range of 24-h body temperature rhythm and serum leptin levels provides clues about the underlying mechanism behind the increased heterothermy in large mammals facing food restriction.

Viral dynamics and immune responses to foot-and-mouth disease virus in African buffalo (Syncerus caffer).

Foot-and-mouth disease (FMD) is one of the most important livestock diseases restricting international trade. While African buffalo (Syncerus caffer) act as the main wildlife reservoir, viral and immune response dynamics during FMD virus acute infection have not been described before in this species. We used experimental needle inoculation and contact infections with three Southern African Territories serotypes to assess clinical, virological and immunological dynamics for thirty days post infection. Clinical FMD in the needle inoculated buffalo was mild and characterised by pyrexia. Despite the absence of generalised vesicles, all contact animals were readily infected with their respective serotypes within the first two to nine days after being mixed with needle challenged buffalo. Irrespective of the route of infection or serotype, there were positive associations between the viral loads in blood and the induction of host innate pro-inflammatory cytokines and acute phase proteins. Viral loads in blood and tonsil swabs were tightly correlated during the acute phase of the infection, however, viraemia significantly declined after a peak at four days post-infection (dpi), which correlated with the presence of detectable neutralising antibodies. In contrast, infectious virus was isolated in the tonsil swabs until the last sampling point (30 dpi) in most animals. The pattern of virus detection in serum and tonsil swabs was similar for all three serotypes in the direct challenged and contact challenged animals. We have demonstrated for the first time that African buffalo are indeed systemically affected by FMD virus and clinical FMD in buffalo is characterized by a transient pyrexia. Despite the lack of FMD lesions, infection of African buffalo was characterised by high viral loads in blood and oropharynx, rapid and strong host innate and adaptive immune responses and high transmissibility.

Biologging subcutaneous temperatures to detect orientation to solar radiation remotely in savanna antelope.

Observations of animal thermoregulatory behavior are labor-intensive, and human presence may disturb the normal behavior of the animal. Therefore, we investigated whether a remote biologging technique could be used to detect orientation to solar radiation in savanna antelope. We predicted that when a mammal was orientated perpendicular to solar radiation, the subcutaneous temperature on the side of the body facing the sun would be greater than that on the opposite side, whereas when the mammal was orientated parallel to solar radiation, subcutaneous temperatures on both sides would be similar. A pilot study showed that the difference between left- and right-side temperatures under a pelt reflected orientation to solar radiation if a pelt-covered cylinder had been orientated for 15 min or longer. In addition, the rate of change in temperature difference could detect orientation that had changed within the previous 5 min. We implanted temperature-sensitive data loggers subcutaneously into the flanks of eight black (Connochaetes gnu) and eight blue (Connochaetes taurinus) wildebeest. By incorporating both the rate of change and subcutaneous temperature differences and excluding times when wildebeest were lying down, our predictions correctly matched behavioral observations of wildebeest orientation to solar radiation 71% of the time. Our technique tended to fail when wildebeest were lying down, wind speeds were high and the sun was overhead. But those are conditions in which the benefits of manipulating orientation to solar radiation is of diminishing importance to a free-living mammal. Therefore, subcutaneous temperatures provide physiologically relevant information on the importance of solar radiation to mammals.