RESUMO
Anthracyclines are used in breast cancer both in early and advanced stages and their recommendation together with taxanes, either concurrently or sequentially, is debatable and individualized by phenotype. Circulating biomarkers have already been introduced in clinical practice for metastatic disease monitoring. We questioned whether it might be a role for these markers in neoadjuvant and adjuvant settings too and a general review was conducted. CK18 and CTC were found predictive for anthracycline related response in preoperative setting. Soluble E-cadherin is promising, a retrospective analysis showing a direct correlation with clinical response. CEA, CA 15-3 and HER2 ECD are not of interest for their predictive role.
RESUMO
An experimental latent herpes infection was established by intradermal inoculation of the mouse ear or of the footpad with a wild strain and a temperature-sensitive (ts) mutant of herpes virus type 2. The wild strain was latency-positive irrespective of the inoculation site; the ts mutant induced latent ganglionic infection only when inoculated in the footpad. Neutralizing antibodies could be detected only after inoculation of the wild strain. The establishment of latency was not prevented by the presence of either a humoral or a cell-mediated immune response.
Assuntos
Herpes Simples/imunologia , Animais , Anticorpos Antivirais/análise , Formação de Anticorpos , Imunidade Celular , Interferon gama/análise , Fatores Inibidores da Migração de Macrófagos/análise , Camundongos , Mutação , Testes de Neutralização , Simplexvirus/imunologia , Simplexvirus/patogenicidade , Baço/imunologiaRESUMO
The cell growth inhibitory action of rat interferon (IFN) was investigated in two systems: a cell line obtained by transformation of rat embryo fibroblasts under the action of a ts mutant of herpes simplex virus and a tumor cell line derived from tumors induced in rats by transformed cells. The growth inhibitory effect of IFN, evaluated by cell counting and colony formation in soft agar medium, was dependent on the exponential cell growth rate and on the concentration of IFN in the medium. Long-time subcultivation of the two cell lines in the presence of IFN did not result in the selection of a cell population resistant to the growth inhibitory effect of IFN, but led to a decrease in the oncogenic potency of the tumor cell line.
Assuntos
Divisão Celular , Transformação Celular Neoplásica , Transformação Celular Viral , Interferons/farmacologia , Animais , Linhagem Celular , Células Clonais , Relação Dose-Resposta a Droga , Neoplasias Experimentais/etiologia , Ratos , Ratos Endogâmicos , Simplexvirus/fisiologia , Fatores de TempoRESUMO
Transformation of rat embryo fibroblast was induced by a temperature-sensitive mutant of herpes simplex virus type 2. Transformation was scored using the morphological criterion of focus formation and the cell changes that lead to the final focus are described. Virus genome persistence in the transformed cell line was demonstrated by the presence of virus-specific membrane antigens and by the specificity of antibodies elicited in rabbits by inoculation of transformed cells. The cytogenetic analysis of the transformed cells revealed changes in chromosome number and the presence of marker chromosomes.
