RESUMO
The hypothesis of new class I antigens has been postulated in man, and several antigen systems have been proposed: HT (Gazit), TC (TCA, TCB) (Van Leeuwen). The present study describes a new class I antigenic marker system, expressed selectively on PHA-activated T lymphocytes and on lymphoblastoid B cell lines. These markers correlated at the cellular activation stage, have been called: human activation or HA markers. 7% of the sera from multiparous women present anti-HA antibodies. The definition of class I molecule (dimer 41K - 12K) has been established by a structural analysis of the molecule; the responsible gene, located on the 6th chromosome could be close to the HLA-A gene. The equivalence with the mouse Qa markers is postulated, but remains to be totally demonstrated.
Assuntos
Antígenos HLA/análise , Linfócitos B/imunologia , Feminino , Antígenos HLA/genética , Antígenos HLA/imunologia , Antígenos HLA-A , Humanos , Técnicas de Imunoadsorção , Isoanticorpos/análise , Leucemia Linfoide/imunologia , Substâncias Macromoleculares , Linfócitos T/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Linfócitos T Reguladores/imunologia , Acetato de Tetradecanoilforbol/farmacologiaRESUMO
An anti-HLA-B27 monoclonal antibody produced by the hybridoma technique is described. This BD.7 reagent is a cytotoxic IgM antibody. Its reactivity was studied by lymphocytotoxicity tests, indirect immunofluorescence tests and biochemical analysis against an extensive panel of peripheral blood mononuclear cells. All HLA-B27 positive samples, either from normal subjects or from patients with Ankylosing Spondylitis, were recognized by this reagent. Moreover, a cross-reaction was observed with HLA-B13 cells, and a new unexpected reaction with all HLA-B37 cell suspensions. The interest of such a reagent is discussed.
Assuntos
Epitopos/imunologia , Antígenos HLA/imunologia , Antígenos HLA-B , Anticorpos Monoclonais/imunologia , Reações Cruzadas , Testes Imunológicos de Citotoxicidade , Imunofluorescência , Antígeno HLA-B13 , Antígeno HLA-B27 , Antígeno HLA-B37 , HumanosRESUMO
New class I antigens in linkage disequilibrium with HLA-A antigen are demonstrated in PHA T and EBV preferential target cells using human alloantisera. These new antigens are defined as class I antigens by immunoprecipitation of a 41-12 k dimer. The molecule is shown to be distinct from the HLA-A, -B, -C molecule and in particular from the A3 molecule as in sequential immunoprecipitations, the depletion of the HLA-A, -B, -C molecule or A3 molecule (44-12 k) has no effect on the new molecule (41-12 k). Being present on the PHA T cells and lymphoblast lines, these antigens are considered as new epitopes involved in the the cell activation process.
Assuntos
Antígenos HLA/análise , Linfócitos/imunologia , Linfócitos B/imunologia , Células Cultivadas , Criança , DNA/análise , Feminino , Citometria de Fluxo , Antígenos HLA/genética , Humanos , Leucemia Linfoide/imunologia , Linfócitos/citologia , Linfócitos T/imunologiaRESUMO
Monocyte-enriched cell suspensions obtained by a plate adherence method from 57 blood donors were typed by microcytotoxicity for HLA-A, -B and -DR determinants. Parallel assays were performed with autologous unfractionated lymphocytes for HLA-A and -B determinants and with autologous B-lymphocytes for HLA-DR determinants. Correlation coefficients (r values) were calculated for nine HLA-A specificities (r greater than 0.79), 16 HLA-B specificities (r greater than 0.56) and seven HLA-DR specificities (r greater than 0.81). For certain HLA-A and -B specificities detection was less readily achieved on monocytes than on autologous lymphocytes. Extra reactions were observed in some instances. With sera defining HLA-DR specificities, monocytes and B-cells showed reactivity of almost identical strength and reliability.
