RESUMO
Surface tegumental membrane of adult stage Schistosoma mansoni were examined using the latex sphere coated with Concanavalin A (Con A). Wheat germ agglutinin (WGA) and Protein A (PA). Competitive saccharide inhibitors glucose, mannose and methyl alpha-D-glucopyranoside were used for Con A.
Assuntos
Concanavalina A/metabolismo , Látex/metabolismo , Salmonella/metabolismo , Schistosoma mansoni/metabolismo , Proteína Estafilocócica A/metabolismo , Aglutininas do Germe de Trigo/metabolismo , Sítios de Ligação , Rotulagem de MedicamentosRESUMO
The authors describe a case of febrile disease acquired by a nursling in Africa: was it induced by Pl. falciparum or by Babesia sp.? In spite of numerous microscopic and serological appraisals from veterinary and medical authorities, it is difficult to state definitively this subject, but it is more probably a babesiosis of canine or rodent origin.
Assuntos
Babesiose/diagnóstico , Malária/diagnóstico , Animais , Babesiose/parasitologia , Diagnóstico Diferencial , Humanos , Lactente , Malária/parasitologia , Masculino , Plasmodium falciparumRESUMO
Special interaction between Salmonella typhimurium (STM) and Schistosoma mansoni is considered under two complementary aspects, in vivo and in vitro, using scanning and transmission electron microscopy. The resulting observations have obviously permitted us to discover a large specificity in these adhesion phenomena that seems to lead to the fusion of membranes between the two organisms. Microanalysis trials executed in these areas of strong affinity were attempted.
Assuntos
Salmonella typhimurium/patogenicidade , Schistosoma mansoni/patogenicidade , Animais , Microscopia Eletrônica de Varredura , Salmonella typhimurium/ultraestrutura , Schistosoma mansoni/ultraestrutura , Esquistossomose/parasitologiaRESUMO
Relations between Schistosoma mansoni and Salmonella typhimurium are studied in vivo and in vitro using scanning and transmission electron microscopy as complementary methods. Salmonellae adhesion is a specific process materialized in special places of male and mature schistosome tegumental surface. Interactions are marked by bacterial strong fibres creating a network all around Schistosoma where Salmonellae are dividing. Membrane junction is the last stage leading to symbiotic balance between two biologic systems.
Assuntos
Salmonella typhimurium/ultraestrutura , Schistosoma mansoni/parasitologia , Animais , Masculino , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Schistosoma mansoni/ultraestrutura , SimbioseRESUMO
Studies performed in vivo and in vitro, using scanning electron microscopy, on relations between Schistosoma mansoni and some enterobacteria show a strong association for certain bacterial strains (Salmonella typhimurium). Salmonellae adhesion on tegumental surface of male and mature Schistosoma is only possible in vitro owing to bacterial synthesis of strong fibres penetrating inside the worm. These interactions are not found with Escherichia coli, Proteus mirabilis or with young schistosomula.
Assuntos
Escherichia coli/fisiologia , Proteus mirabilis/fisiologia , Salmonella typhimurium/fisiologia , Schistosoma mansoni/microbiologia , Adesividade , Animais , Enterobacteriaceae/ultraestrutura , Feminino , Masculino , Microscopia Eletrônica de Varredura , Schistosoma mansoni/crescimento & desenvolvimento , Schistosoma mansoni/ultraestrutura , Especificidade da EspécieRESUMO
The adult stages of Litosomosoides carinii, Dirofilaria uniformis and Dirofilaria immitis have been successfully maintained in vitro though microfilaria production by the worms continued only for a period of one to 18 days. In this paper we describe the results obtained in a series of experiments in which adult L1 and L2 stages of Dipetalonema viteae were maintained in vitro.
Assuntos
Dipetalonema/ultraestrutura , Animais , Dipetalonema/crescimento & desenvolvimento , Técnicas In Vitro , Larva/ultraestrutura , Microscopia Eletrônica de VarreduraRESUMO
From our observation in vitro, we can suggest that : a direct contact between sarcomatous cells and physiologically active yeasts appears to be necessary for the transfer of the radioactive labelling; the presence of a filter with pores of 1.2 micrometer diameter prevents passage of fragments of DNA or RNA between the cells; this transfer is a very small fact, but implies about the third of the yeasts.