The effect of warming and seasonality on bioaccumulation of selected pharmaceuticals in freshwater invertebrates.

Multiple human-induced environmental stressors significantly threaten global biodiversity and ecosystem functioning. Climate warming and chemical pollution are two widespread stressors whose impact on freshwaters is likely to increase. However, little is known about the combined effects of warming on the bioaccumulation of environmentally relevant mixtures of emerging contaminants, such as pharmaceutically active compounds (PhACs) in freshwater biota. This study investigated the bioaccumulation of a mixture of 15 selected PhACs at environmentally relevant concentrations in common freshwater macroinvertebrate taxa, exposed to ambient temperatures and warming (+4 °C) during the warm and cold seasons in two outdoor mesocosm experiments. Nine PhACs (carbamazepine, cetirizine, clarithromycin, clindamycin, fexofenadine, telmisartan, trimethoprim, valsartan and venlafaxine) were dissipated faster in the warm season experiment than in the cold season experiment, while lamotrigine showed the opposite trend. The most bioaccumulated PhACs in macroinvertebrates were tramadol, carbamazepine, telmisartan, venlafaxine, citalopram and cetirizine. The bioaccumulation was taxon, season and temperature dependent, but differences could not be fully explained by the different water stability of the PhACs and their partitioning between water and leaf litter. The highest water-based bioaccumulation factors were found in Asellus and Planorbarius. Moreover, the bioaccumulation of some PhACs increased with warming in Planorbarius, suggesting that it could be used as a sentinel taxon in environmental studies of the effects of climate warming on PhAC bioaccumulation.

Length-mass allometries of the larvae of aquatic dipterans: differences between taxa, morphological traits, and methods.

Body mass underpins many ecological processes at the level of individuals, populations, and communities. Often estimated in arthropods from linear morphological traits such as body length or head width, these relationships can vary even between closely related taxa. Length-mass relationships of mosquito (Diptera: Culicidae) larvae are poorly known despite the importance of this family to disease and aquatic ecology. To fill this gap, we measured ontogenetic changes in linear traits (body length, head width, and thorax width) and dry and wet masses and estimated length- and width-mass relationships in larvae of 3 culicid species inhabiting different niches: the tropical Aedes albopictus (Skuse, 1894), the temperate Culex pipiens (Linnaeus, 1758), and the snowmelt Ochlerotatus punctor (Kirby, 1837). We compared our results with published length-mass allometries of other aquatic dipteran larvae. We showed that thorax width and body length, but not head width, reliably predicted body mass for our 3 species. The length-mass allometry slopes in aquatic dipterans varied considerably between and within families but were independent of phylogeny, specimen handling, preservation techniques, and data fitting methods. Slope estimates became less precise with decreasing sample size and size range. To obtain reliable estimates of the allometric slopes, we have thus recommended using data on all larval stages for intraspecific allometries and a wide range of species for interspecific allometries. We also cautioned against the indiscriminate use of length-mass allometries obtained for other taxa or collected at lower taxonomic resolutions, e.g., when using length-mass relationships to estimate biomass production at a given site.

Body size and trophic position determine the outcomes of species invasions along temperature and productivity gradients.

Species invasions are predicted to increase in frequency with global change, but quantitative predictions of how environmental filters and species traits influence the success and consequences of invasions for local communities are lacking. Here we investigate how invaders alter the structure, diversity and stability regime of simple communities across environmental gradients (habitat productivity, temperature) and community size structure. We simulate all three-species trophic modules (apparent and exploitative competition, trophic chain and intraguild predation). We predict that invasions most often succeed in warm and productive habitats and that successful invaders include smaller competitors, intraguild predators and comparatively small top predators. This suggests that species invasions and global change may facilitate the downsizing of food webs. Furthermore, we show that successful invasions leading to species substitutions rarely alter system stability, while invasions leading to increased diversity can destabilize or stabilize community dynamics depending on the environmental conditions and invader's trophic position.

Combined effects of climate warming and pharmaceuticals on a tri-trophic freshwater food web.

Multiple anthropogenic stressors influence the functioning of lakes and ponds, but their combined effects are often little understood. We conducted two mesocosm experiments to evaluate the effects of warming (+4 °C above ambient temperature) and environmentally relevant concentrations of a mixture of commonly used pharmaceuticals (cardiovascular, psychoactive, antihistamines, antibiotics) on tri-trophic food webs representative of communities in ponds and other small standing waters. Communities were constituted of phyto- and zooplankton and macroinvertebrates (molluscs and insects) including benthic detritivores, grazers, omnivorous scrapers, omnivorous piercers, water column predators, benthic predators, and phytophilous predators. We quantified the main and interactive effects of warming and pharmaceuticals on each trophic level in the pelagic community and attributed them to the direct effects of both stressors and the indirect effects arising through biotic interactions. Warming and pharmaceuticals had stronger effects in the summer experiment, altering zooplankton community composition and causing delayed or accelerated emergence of top insect predators (odonates). In the summer experiment, both stressors and top predators reduced the biomass of filter-feeding zooplankton (cladocerans), while warming and pharmaceuticals had opposite effects on phytoplankton. In the winter experiment, the effects were much weaker and were limited to a positive effect of warming on phytoplankton biomass. Overall, we show that pharmaceuticals can exacerbate the effects of climate warming in freshwater ecosystems, especially during the warm season. Our results demonstrate the utility of community-level studies across seasons for risk assessment of multiple emerging stressors in freshwater ecosystems.

Bioaccumulation of chemical elements at post-industrial freshwater sites varies predictably between habitats, elements and taxa: A power law approach.

Elevated environmental levels of elements originating from anthropogenic activities threaten natural communities and public health, as these elements can persist and bioaccumulate in the environment. However, their environmental risks and bioaccumulation patterns are often habitat-, species- and element-specific. We studied the bioaccumulation patterns of 11 elements in seven freshwater taxa in post-mining habitats in the Czech Republic, ranging from less polluted mining ponds to highly polluted fly ash lagoons. We found nonlinear, power-law relationships between the environmental and tissue concentrations of the elements, which may explain differences in bioaccumulation factors (BAF) reported in the literature. Tissue concentrations were driven by the environmental concentrations in non-essential elements (Al, As, Co, Cr, Ni, Pb and V), but this dependence was limited in essential elements (Cu, Mn, Se and Zn). Tissue concentrations of most elements were also more closely related to substrate than to water concentrations. Bioaccumulation was habitat specific in eight elements: stronger in mining ponds for Al and Pb, and stronger in fly ash lagoons for As, Cu, Mn, Pb, Se, V and Zn, although the differences were often minor. Bioaccumulation of some elements further increased in mineral-rich localities. Proximity to substrate, rather than trophic level, drove increased bioaccumulation levels across taxa. This highlights the importance of substrate as a pollutant reservoir in standing freshwaters and suggests that benthic taxa, such as molluscs (e.g., Physella) and other macroinvertebrates (e.g., Nepa), constitute good bioindicators. Despite the higher environmental risks in fly ash lagoons than in mining ponds, the observed ability of freshwater biota to sustain pollution supports the conservation potential of post-industrial sites. The power law approach used here to quantify and disentangle the effects of various bioaccumulation drivers may be helpful in additional contexts, increasing our ability to predict the effects of other contaminants and environmental hazards on biota.

Muddying the unexplored post-industrial waters: Biodiversity and conservation potential of freshwater habitats in fly ash sedimentation lagoons.

Deposits of fly ash and other coal combustion wastes are common remnants of the energy industry. Despite their environmental risks from heavy metals and trace elements, they have been revealed as refuges for threatened terrestrial biodiversity. Surprisingly, freshwater biodiversity of fly ash sedimentation lagoons remains unknown despite such lack of knowledge strongly limits the efficient restoration of fly ash deposits. We bring the first comprehensive survey of freshwater biodiversity, including nekton, benthos, zooplankton, phytoplankton, and macrophytes, in fly ash lagoons across industrial regions of the Czech Republic. To assess their conservation potential, we compared their biodiversity with abandoned post-mining ponds, the known strongholds of endangered aquatic species in the region with a shortage of natural ponds. Of 28 recorded threatened species, 15 occurred in the studied fly ash lagoons, some of which were less abundant or even absent in the post-mining ponds. These are often species of nutrient-poor, fishless waters with rich vegetation, although some are specialised extremophiles. Species richness and conservation value of most groups in the fly ash lagoons did not significantly differ from the post-mining ponds, except for species richness of benthos, zooplankton, and macrophytes, which were slightly lower in the fly ash lagoons. Although the concentrations of some heavy metals (mainly Se, V, and As) were significantly higher in the fly ash lagoons, they did not significantly affect species richness or conservation value of the local communities. The differences in species composition therefore does not seem to be caused by water chemistry. Altogether, we have shown that fly ash lagoons are refuges for threatened aquatic species, and we thus suggest maintaining water bodies during site restoration after the cessation of fly ash deposition. Based on our analyses of environmental variables, we discuss suitable restoration practices that efficiently combine biodiversity protection and environmental risk reduction.

An evolutionary explanation of female-biased sexual size dimorphism in North Sea plaice, Pleuronectes platessa L.

Sexual size dimorphism (SSD) is caused by differences in selection pressures and life-history trade-offs faced by males and females. Proximate causes of SSD may involve sex-specific mortality, energy acquisition, and energy expenditure for maintenance, reproductive tissues, and reproductive behavior. Using a quantitative, individual-based, eco-genetic model parameterized for North Sea plaice, we explore the importance of these mechanisms for female-biased SSD, under which males are smaller and reach sexual maturity earlier than females (common among fish, but also arising in arthropods and mammals). We consider two mechanisms potentially serving as ultimate causes: (a) Male investments in male reproductive behavior might evolve to detract energy resources that would otherwise be available for somatic growth, and (b) diminishing returns on male reproductive investments might evolve to reduce energy acquisition. In general, both of these can bring about smaller male body sizes. We report the following findings. First, higher investments in male reproductive behavior alone cannot explain the North Sea plaice SSD. This is because such higher reproductive investments require increased energy acquisition, which would cause a delay in maturation, leading to male-biased SSD contrary to observations. When accounting for the observed differential (lower) male mortality, maturation is postponed even further, leading to even larger males. Second, diminishing returns on male reproductive investments alone can qualitatively account for the North Sea plaice SSD, even though the quantitative match is imperfect. Third, both mechanisms can be reconciled with, and thus provide a mechanistic basis for, the previously advanced Ghiselin-Reiss hypothesis, according to which smaller males will evolve if their reproductive success is dominated by scramble competition for fertilizing females, as males would consequently invest more in reproduction than growth, potentially implying lower survival rates, and thus relaxing male-male competition. Fourth, a good quantitative fit with the North Sea plaice SSD is achieved by combining both mechanisms while accounting for sex-specific costs males incur during their spawning season. Fifth, evolution caused by fishing is likely to have modified the North Sea plaice SSD.

Effects of a microplastic mixture differ across trophic levels and taxa in a freshwater food web: In situ mesocosm experiment.

The ubiquitous presence of microplastics (MP) in aquatic ecosystems can affect organisms and communities in multiple ways. While MP research on aquatic organisms has primarily focused on marine ecosystems and laboratory experiments, the community-level effects of MP in freshwaters, especially in lakes, are poorly understood. To examine the impact of MP on freshwater lake ecosystems, we conducted the first in situ community-level mesocosm experiment testing the effects of MP on a model food web with zooplankton as main herbivores, odonate larvae as predators, and chironomid larvae as detritivores for seven weeks. The mesocosms were exposed to a mixture of the most abundant MP polymers found in freshwaters, added at two different concentrations in a single pulse to the water surface, water column and sediment. Water column MP concentrations declined sharply during the first two weeks of the experiment. Contrary to expectations, MP ingestion by zooplankton was low and limited mainly to large-bodied Daphnia, causing a decrease in biomass. Biomass of the other zooplankton taxa did not decrease. Presence of MP in the faecal pellets of odonate larvae that fed on zooplankton was indicative of a trophic transfer of MP. The results demonstrated that MP ingestion varies predictably with MP size, as well as body size and feeding preference of the organism, which can be used to predict the rates of transfer and further effects of MP on freshwater food webs. For chironomids, MP had only a low, short-term impact on emergence patterns while their wing morphology was significantly changed. Overall, the impact of MP exposure on the experimental food web and cross-ecosystem biomass transfer was lower than expected, but the experiment provided the first in situ observation of MP transfer to terrestrial ecosystems by emerging chironomids.

Community structure and collapses in multichannel food webs: Role of consumer body sizes and mesohabitat productivities.

Multichannel food webs are shaped by the ability of apex predators to link asymmetric energy flows in mesohabitats differing in productivity and community traits. While body size is a fundamental trait underlying life histories and demography, its implications for structuring multichannel food webs are unexplored. To fill this gap, we develop a model that links population responses to predation, and resource availability to community-level patterns, using a tri-trophic food web model with two populations of intermediate consumers and a size-selective top predator. We show that asymmetries in mesohabitat productivities and consumer body sizes drive food web structure, merging previously separate theory on apparent competition and emergent Allee effects (i.e. abrupt population collapses) of top predators. Our results yield theoretical support for empirically observed stability of asymmetric multichannel food webs and discover three novel types of emergent Allee effects involving intermediate consumers, multiple populations or multiple alternative stable states.

Disentangling the nonlinear effects of habitat complexity on functional responses.

Structural complexity of habitats modifies trophic interactions by providing refuges and altering predator and prey behaviour. Nonlinear effects on trophic interaction strengths driven by these mechanisms may alter food web dynamics and community structure in response to habitat modifications. However, changes in functional response, the relationship between prey density and feeding rate, along habitat complexity (HC) gradients are little understood. We quantified functional responses along a HC gradient from an entirely unstructured to highly structured habitat in a freshwater system, using dragonfly larvae (Aeshna cyanea) preying on Chaoborus obscuripes larvae. To disentangle mechanisms by which changes in HC affect functional responses, we used two different approaches-a population-level and a behavioural experiment-applied an information theoretic approach to identify plausible links between HC and functional response parameters, and compared our results to previous studies. Functional response shape did not change, but we found strong evidence for nonlinear dependence of attack rate and handling time on HC in our study. Combined results from both experiments imply that attack rate increased stepwise between the unstructured and structured habitats in line with the threshold hypothesis, because the predators gained better access to the prey. Handling time was lowest at an intermediate HC level in the population-level experiment while the direct estimate of handling time did not vary with HC in the behavioural experiment. These differences point towards HC-driven changes in foraging activity and other predator and prey behaviour. Most previous studies reported stepwise decrease in attack rate in line with the threshold hypothesis or no change with increasing HC. Moreover, changes in the handling time parameter with HC appear to be relatively common but not conforming to the threshold hypothesis. Overall, increased HC appears to, respectively, weaken and strengthen trophic links in 2D and 3D predator-prey interactions. We conclude that detailed understanding of HC effects on food webs requires complementary experimental approaches across HC gradients that consider predator foraging strategies and predator and prey behaviour. Such studies can also help guide conservation efforts as addition of structural elements is frequently used for restoration of degraded aquatic habitats.

Impacts of predator-induced behavioural plasticity on the temperature dependence of predator-prey activity and population dynamics.

Predation is a key ecological interaction affecting populations and communities. Climate warming can modify this interaction both directly by the kinetic effects of temperature on biological rates and indirectly through integrated behavioural and physiological responses of the predators and prey. Temperature dependence of predation rates can further be altered by predator-induced plasticity of prey locomotor activity, but empirical data about this effect are lacking. We propose a general framework to understand the influence of predator-induced developmental plasticity on behavioural thermal reaction norms in prey and their consequences for predator-prey dynamics. Using a mesocosm experiment with dragonfly larvae (predator) and newt larvae (prey), we tested if the predator-induced plasticity alters the elevation or the slope of the thermal reaction norms for locomotor activity metrics in prey. We also estimated the joint predator-prey thermal response in mean locomotor speed, which determines prey encounter rate, and modelled the effect of both phenomena on predator-prey population dynamics. Thermal reaction norms for locomotor activity in prey were affected by predation risk cues but with minor influence on the joint predator-prey behavioural response. We found that predation risk cues significantly decreased the intercept of thermal reaction norm for total activity rate (i.e. all body movements) but not the other locomotor activity metrics in the prey, and that prey locomotor activity rate and locomotor speed increased with prey density. Temperature had opposite effects on the mean relative speed of predator and prey as individual speed increased with temperature in predators but decreased in prey. This led to a negligible effect of body temperature on predicted prey encounter rates and predator-prey dynamics. The behavioural component of predator-prey interaction varied much more between individuals than with temperature and the presence of predation risk cues in our system. We conclude that within-population variation in locomotor activity can buffer the influence of body temperature and predation risk cues on predator-prey interactions, and further research should focus on the magnitude and sources of behavioural variation in interacting species to predict the impact of climate change on predator-prey interactions and food web dynamics.

Meta-analysis of multidecadal biodiversity trends in Europe.

Local biodiversity trends over time are likely to be decoupled from global trends, as local processes may compensate or counteract global change. We analyze 161 long-term biological time series (15-91 years) collected across Europe, using a comprehensive dataset comprising ~6,200 marine, freshwater and terrestrial taxa. We test whether (i) local long-term biodiversity trends are consistent among biogeoregions, realms and taxonomic groups, and (ii) changes in biodiversity correlate with regional climate and local conditions. Our results reveal that local trends of abundance, richness and diversity differ among biogeoregions, realms and taxonomic groups, demonstrating that biodiversity changes at local scale are often complex and cannot be easily generalized. However, we find increases in richness and abundance with increasing temperature and naturalness as well as a clear spatial pattern in changes in community composition (i.e. temporal taxonomic turnover) in most biogeoregions of Northern and Eastern Europe.

Changing environmental conditions underpin long-term patterns of phytoplankton in a freshwater reservoir.

Environmental changes can exert strong pressure on freshwater biota and lead to unwanted alterations of local communities and deterioration of ecosystem services. Disentangling the links between environmental and community changes is, therefore, essential to understand and predict the impact of human activities on freshwater ecosystems. This is particularly relevant for man-made freshwater reservoirs that represent a nexus between anthropogenic, environmental, and biotic effects. Reservoir food webs depend strongly on phytoplankton dynamics, which are affected by abiotic conditions, nutrient availability and grazing pressure by zooplankton. We studied the effects of relevant environmental drivers (hydrochemistry, hydrodynamics and zooplankton) on the composition, diversity and community stability of main morpho-functional phytoplankton groups over 32 years in the Rímov Reservoir (Czech Republic). Environmental conditions in the reservoir are characterised by three distinct periods (1983-89, 1990-99, and 2000-14) defined by shifts and breakpoints in temporal trends in reservoir hydrochemistry and hydraulic conditions, and we examined if and how phytoplankton responded to these abrupt changes. We found significant differences in phytoplankton composition among the three periods. Phytoplankton underwent a substantial compositional shift towards a dominance of pennate diatoms. Time-lag analysis of dissimilarity in phytoplankton composition revealed higher and stochastic annual variations until 1999, followed by a lower variability and divergence in phytoplankton composition in subsequent years. Changes in overall phytoplankton assemblage and most abundant morpho-functional phytoplankton groups were driven mainly by hydrochemical (total nitrogen) and hydrodynamic variables (inflow rate, surface level and mixing depth) and less by zooplankton dynamics. These results suggest that phytoplankton are driven primarily by nutrient input and water regime, both of which can be appropriately managed to support valuable ecosystem services provided by phytoplankton in freshwater reservoirs.

Prey selectivity and the effect of diet on growth and development of a dragonfly, Sympetrum sanguineum.

Despite a long tradition of research, our understanding of mechanisms driving prey selectivity in predatory insects is limited. According to optimal foraging theory, predators should prefer prey which provides the highest amount of energy per unit time. However, prey selectivity may also depend on previous diet and specific nutritional demands of the predator. From the long-term perspective, diet composition affects predator fitness. An open question is whether short-term selectivity of predators provides a diet which is optimal in the long-term. To shed more light on these issues, we conducted laboratory experiments on prey selectivity and its long-term consequences in larvae of the dragonfly Sympetrum sanguineum. We conditioned the larvae to one of two prey types, the cladoceran Daphnia magna and larvae of a non-biting midge Chironomus sp., and then exposed them to various combinations of the two prey types. We found that dragonfly larvae conditioned to Chironomus larvae consumed the same amount of D. magna, but significantly less Chironomus larvae compared to dragonfly larvae conditioned to D. magna. However, there was no effect of previous diet on their success of capture and handling time, suggesting a limited role of learning in their ability to process prey. We then tested the long-term effects of diets with different proportions of both prey for survival and growth of the dragonfly larvae. Individuals fed Chironomus-only diet had higher mortality and slower growth than dragonflies fed D. magna, while larvae fed a mixed diet had the highest survival and growth rate. In conclusion, we show that dragonfly larvae fed by Chironomus larvae performed poorly and compensated by preferring D. magna when both prey types were available. The superiority of the mixed diet suggests that a diverse diet may be needed to satisfy nutritional demands in S. sanguineum larvae. We demonstrate that merging short-term predation experiments with relevant data on predator fitness may provide better understanding of predator-prey interactions and conclude that detailed information on the (mis)matches between prey composition and predator nutritional demands is needed for further progress.

Species interactions under climate change: connecting kinetic effects of temperature on individuals to community dynamics.

Human-induced climate change, dominated by warming trends, poses a major threat to global biodiversity and ecosystem functioning. Species interactions relay the direct and indirect effects of climate warming on individuals to communities, and detailed understanding across these levels is crucial to predict ecological consequences of climate change. We provide a conceptual framework that links temperature effects on insect physiology and behaviour to altered species interactions and community dynamics. We highlight key features of this framework with recent studies investigating the impacts of warming climate on insects and other ectotherms and identify methodological, taxonomic and geographic biases. While the effects of increased constant temperatures are now well understood, future studies should focus on temperature variation, interactions with other stressors and cross-system comparisons.

On the use of functional responses to quantify emergent multiple predator effects.

Non-independent interactions among predators can have important consequences for the structure and dynamics of ecological communities by enhancing or reducing prey mortality rate through, e.g., predator facilitation or interference. The multiplicative risk model, traditionally used to detect these emergent multiple predator effects (MPEs), is biased because it assumes linear functional response (FR) and no prey depletion. To rectify these biases, two approaches based on FR modelling have recently been proposed: the direct FR approach and the population-dynamic approach. Here we compare the strengths, limitations and predictions of the three approaches using simulated data sets. We found that the predictions of the direct FR and the multiplicative risk models are very similar and underestimate predation rates when prey density is high or prey depletion is substantial. As a consequence, these two approaches often fail in detecting risk reduction. Finally, parameters estimated with the direct FR approach lack mechanistic interpretation, which limits the understanding of the mechanisms driving multiple predator interactions and potential extension of this approach to more complex food webs. We thus strongly recommend using the population-dynamic approach because it is robust, precise, and provides a scalable mechanistic framework to detect and quantify MPEs.

Seasonality and weather conditions jointly drive flight activity patterns of aquatic and terrestrial chironomids.

BACKGROUND: Chironomids, a major invertebrate taxon in many standing freshwaters, rely on adult flight to reach new suitable sites, yet the impact of weather conditions on their flight activity is little understood. We investigated diel and seasonal flight activity patterns of aquatic and terrestrial chironomids in a reclaimed sandpit area and analysed how weather conditions and seasonality influenced their total abundance and species composition. RESULTS: Air temperature, relative humidity, wind speed, and air pressure significantly affected total flight activity of both groups, but not in the same way. We identified an intermediate temperature and humidity optimum for the flight activity of terrestrial chironomids, which contrasted with weaker, timescale-dependent relationships in aquatic species. Flight activity of both groups further declined with wind speed and increased with air pressure. Observed flight patterns also varied in time on both daily and seasonal scale. Flight activity of both groups peaked in the evenings after accounting for weather conditions but, surprisingly, aquatic and terrestrial chironomids used partly alternating time windows for dispersal during the season. This may be driven by different seasonal trends of key environmental variables in larval habitats and hence implies that species phenologies and conditions experienced by chironomid larvae (and probably other aquatic insects with short-lived adults) influence adult flight patterns more than weather conditions. CONCLUSIONS: Our results provide detailed insights into the drivers of chironomid flight activity and highlight the methodological challenges arising from the inherent collinearity of weather characteristics and their diurnal and seasonal cycles.

Effects of prey density, temperature and predator diversity on nonconsumptive predator-driven mortality in a freshwater food web.

Nonconsumptive predator-driven mortality (NCM), defined as prey mortality due to predation that does not result in prey consumption, is an underestimated component of predator-prey interactions with possible implications for population dynamics and ecosystem functioning. However, the biotic and abiotic factors influencing this mortality component remain largely unexplored, leaving a gap in our understanding of the impacts of environmental change on ecological communities. We investigated the effects of temperature, prey density, and predator diversity and density on NCM in an aquatic food web module composed of dragonfly larvae (Aeshna cyanea) and marbled crayfish (Procambarus fallax f. virginalis) preying on common carp (Cyprinus carpio) fry. We found that NCM increased with prey density and depended on the functional diversity and density of the predator community. Warming significantly reduced NCM only in the dragonfly larvae but the magnitude depended on dragonfly larvae density. Our results indicate that energy transfer across trophic levels is more efficient due to lower NCM in functionally diverse predator communities, at lower resource densities and at higher temperatures. This suggests that environmental changes such as climate warming and reduced resource availability could increase the efficiency of energy transfer in food webs only if functionally diverse predator communities are conserved.

Temperature-size responses alter food chain persistence across environmental gradients.

Body-size reduction is a ubiquitous response to global warming alongside changes in species phenology and distributions. However, ecological consequences of temperature-size (TS) responses for community persistence under environmental change remain largely unexplored. Here, we investigated the interactive effects of warming, enrichment, community size structure and TS responses on a three-species food chain using a temperature-dependent model with empirical parameterisation. We found that TS responses often increase community persistence, mainly by modifying consumer-resource size ratios and thereby altering interaction strengths and energetic efficiencies. However, the sign and magnitude of these effects vary with warming and enrichment levels, TS responses of constituent species, and community size structure. We predict that the consequences of TS responses are stronger in aquatic than in terrestrial ecosystems, especially when species show different TS responses. We conclude that considering the links between phenotypic plasticity, environmental drivers and species interactions is crucial to better predict global change impacts on ecosystem diversity and stability.

Predator diversity and environmental change modify the strengths of trophic and nontrophic interactions.

Understanding the dependence of species interaction strengths on environmental factors and species diversity is crucial to predict community dynamics and persistence in a rapidly changing world. Nontrophic (e.g. predator interference) and trophic components together determine species interaction strengths, but the effects of environmental factors on these two components remain largely unknown. This impedes our ability to fully understand the links between environmental drivers and species interactions. Here, we used a dynamical modelling framework based on measured predator functional responses to investigate the effects of predator diversity, prey density, and temperature on trophic and nontrophic interaction strengths within a freshwater food web. We found that (i) species interaction strengths cannot be predicted from trophic interactions alone, (ii) nontrophic interaction strengths vary strongly among predator assemblages, (iii) temperature has opposite effects on trophic and nontrophic interaction strengths, and (iv) trophic interaction strengths decrease with prey density, whereas the dependence of nontrophic interaction strengths on prey density is concave up. Interestingly, the qualitative impacts of temperature and prey density on the strengths of trophic and nontrophic interactions were independent of predator identity, suggesting a general pattern. Our results indicate that taking multiple environmental factors and the nonlinearity of density-dependent species interactions into account is an important step towards a better understanding of the effects of environmental variations on complex ecological communities. The functional response approach used in this study opens new avenues for (i) the quantification of the relative importance of the trophic and nontrophic components in species interactions and (ii) a better understanding how environmental factors affect these interactions and the dynamics of ecological communities.