[Analysis of some species of magnolia introduced to west georgia, on content of aporphine alkaloids and their biological activity].

The goal of research was study of vegetative organs of Magnolia species introduced to west Georgia on qualitative and quantitative content of aporphine alkaloids and evaluate cytotoxic activity of total alkaloids from M. officinalis and M. glauca against A-549, DLD-1 and WS-1. Qualitative and quantitative content of aporphine alkaloids in different vegetative organs were determined by chromatographic methods. Based on the researches alkaloids - liriodenine and lanuginozine are characteristic for leaves, for bark of branches - liriodenine, d-caaverine; for bark of trunk - a liriodenine, a caaverine and a magnoflorine. Liriodenine may be considered as is chemotaxonomic sign of genus Magnolia, as it was found in all analyzed vegetative organs. In vitro cytotoxic activity of total alkaloids of M. officinalis and M. glauca has been investigated against DLD-1, WS-1 and A-549. Total alkaloids of M. glauca expressed significant cytotoxic activity against DLD-1 and WS-1, and medium cytotoxicity against A-549; M. officinalis exerted middle activity against A-549, DLD-1, WS-1.

[Study on the separation process of pharmacological active total alkaloids from Chelidonium majus L. growing in Georgia].

In present article was studied the separation process of pharmacological active total alkaloids from Chelidonium majus L. growing in Georgia. Alkaloids were extracted from medicinal herbal material and separated by liquid extraction, diluents gas and a microfiltration through membrane equipment. The obtained A1, A2, A3 fractions were analyzed by GC/MS method; in all cases separation proceeds by the principle of extraction of the target alkaloids. It was concluded that the A1 is enriched with α and ß cryptopins, and protopin, but homochelidonine and chelidonine are in low contents. As accompanying alkaloid is identified dihydrosanguinarine as an artifact; the A2 is enriched with the maximum contents of stylopine and protopin, but the poor contents of chelidonine and homochelidonine; the A3 is enriched with α and ß cryptopins and maximum content of chelidonine. Extraction of alkaloids from Chelidonium majus L. proceeds selectively, but depending on a way of separation of the total alkaloids allows varying qualitative and quantitative consistence of the final product.