Oral and genital human herpesvirus 8 and human papillomavirus in heterosexual partners.

BACKGROUND: The purpose of this study was to verify a possible co-infection of human herpesvirus 8 (HHV-8) in commonly associated human papillomavirus (HPV) penile lesions and to determine the frequency of detection of these viruses in the oral mucosa of their female counterparts. METHODS: Thirty-one male subjects underwent penile swabs from clinical HPV-related lesions. Their female counterparts underwent swabs of the vagina, uterine cervix, and oral mucosa. HPV and HHV-8 detection was performed by polymerase chain reaction using the consensus primers MY11/MY09 and KS1/KS2, respectively. RESULTS: HPV DNA was detected in 31/31 penile lesions. HPV DNA was also detected in 18/31 (58%) female genital brushings and 17/31 (54%) female oral brushings. HHV-8 DNA was detected in 1/31 (3.2%) male genital brushings and 3/31 (9.6%) female oral mucosa brushings. None of the female genital brushings were HHV-8 DNA-infected. CONCLUSIONS: Based upon the results of this study, co-infection between HPV and HHV-8 in malignant and pre-malignant penile lesions is an unlikely finding.

Chloroquine-induced hyperpigmentation of the hard palate.

This article reports a rare case of extensive palatal pigmentation secondary to long-term chloroquine treatment. Chloroquine was originally used as an antimalarial agent, but it is now widely used as an adjunct in the treatment of autoimmune diseases. Adverse effects of chloroquine usually include skin changes such as bullous pemphigoid, exacerbation of psoriasis, and pigmentation of the skin and mucous membranes as well as retinopathy, gastrointestinal alterations, and neuromuscular disorders. Extensive oral pigmentation is an uncommon feature of an adverse drug effect, and diagnosis should be based on clinicopathological findings.

Análise da microinfiltração em dentes decíduos utilizando diferentes técnicas adesivas / Microleakage assessment in primary teeth using different adhesive techniques

Objetivo: Avaliar in vitro a microinfiltracao em restauracoes confeccionadas em molares deciduos com diferentes tecnicas adesivas. Metodo: Foram confeccionados preparos cavitarios nas faces vestibular e lingual, divididos aleatoriamente em quatro grupos: G1 (n=10) sistema adesivo autocondicionante (Adper Prompt L-Pop); G2 (n=10) sistema adesivo convencional (Adper Single Bond 2); G3 (n=10) condicionamento com acido fosforico a 37% e sistema adesivo autocondicionante (Self-etch Bond); G4 (n=10) sistema adesivo autocondicionante (Self-etch Bond). Os grupos 1 e 2 foram restaurados com resina composta Concept Advanced (Vigodent) e os grupos 3 e 4 com a resina composta Z100 (3M). Em seguida, procederam-se a ciclagem termica (ñ5o e ñ55oC, 125 ciclos, 10 segundos em cada banho), impermeabilizacao (cola de presa rapida e esmalte de unha), imersao em Fucsina basica a 0,5% e seccionamento no senti do vestibulo-lingual. A avaliacao foi realizada em microscopio estereoscopico, com aumento de 25 vezes, por tres examinadores calibrados, seguindo uma escala preestabelecida (graus de 0 -1). Utilizou se a estatistica descritiva e o teste do Qui-quadrado, com nivel de significancia de 5%. Resultados: As tecnicas adesivas estudadas nao foram capazes de impedir a microinfiltracao. Na margem oclusal, o grupo 4 apresentou maior microinfiltracao com diferenca estatisticamente significante, quando comparado com os demais. Na margem cervical, o grupo 1 apresentou maior microinfiltracao com diferenca estatisticamente significante, quando comparado com os demais. Comparando-se os grupos, independentemente das margens, os grupos 2 e 3 apresentaram melhor performance em relacao a microinfiltracao, com diferenca estatisticamente significante em relacao aos demais. Conclusao: Nenhum dos sistemas adesivos foi capaz de selar completamente as margens cavitarias.

Objective: To evaluate in vitro the microleakage in restorations placed in primary molars using different adhesive techniques. Method: Cavities prepared in the buccal and lingual faces of the teeth were randomly assigned to four groups: G1 (n=10) selfetch adhesive system (Adper Prompt L-Pop); G2 (n=10) etchand- rinse adhesive system (Adper Single Bond 2); G3 (n=10) 37% phosphoric acid etching plus self-etch adhesive system (Self-Etch Bond); G4 (n=10) self-etch adhesive system (Self- Etch Bond). Groups 1 and 2 were restored with the composite resin Concept Advanced (Vigodent), while groups 3 and 4 were restored with the composite resin Z100 (3M). The teeth were subjected to a thermocycling regimen of 125 cycles between ñ5o and ñ 55o with dwell time of 10 seconds, and were rendered waterproof (quick-setting adhesive + nail polish), immersed in 0.5% basic fuchsine and sectioned in a buccolingual direction. The specimens were examined by three calibrated examiners under a stereomicroscope (Coleman) at ž25 magnification, according to a pre-established scoring system (0-1). Descriptive statistical analysis was done and the chi-squared test was used with a significance level of 5%. Results: The adhesive techniques did not prevent the occurrence microleakage. In the occlusal margin, group 4 presented significantly higher microleakage than the other groups. In the cervical margin, group 1 presented significantly higher microleakage than the other groups. Comparing the groups irrespectively of the margins, groups 2 and 3 presented lesser microleakage and differed significantly from the other groups. Conclusion: None of the adhesive systems was capable of sealing the cavity margins completely.

Propriedades e aspectos biológicos do agregado trióxido mineral: revisão da literatura / Properties and biological aspects of mineral trioxide aggregate: literature review

O material obturador ideal deve apresentar características específicas, como biocompatibilidade, selamento marginal adequado, capacidade de permitir ou induzir o reparo ósseo e facilidade de manipulação e inserção. O agregado trióxido mineral (MTA) foi desenvolvido como um material para obturação retrógrada com melhores propriedades químicas, físicas e biológicas do que os materiais existentes na época, como amálgama, material restaurador intermediário (IRM) e SuperEBA. Atualmente, o MTA é usado como material de preenchimento radicular no selamento apical, no reparo de perfurações e como agente de capeamento pulpar, além de ser usado como material de obturação retrógrada. O objetivo deste artigo foi realizar uma revisão da literatura acerca do agregado trióxido mineral, comparando-o com os principais cimentos endodônticos e materiais obturadores retrógrados disponíveis no mercado para a retrobturação do sistema de canais radiculares, analisando suas propriedades e características biológicas. Concluiu-se que o MTA é um material obturador retrógrado bastante satisfatório do ponto de vista químico-físico-biológico, especialmente por sua biocompatibilidade e seu potencial de ação osteocondutora, osteoindutora e cementocondutora.

The ideal root canal sealer should present specific characteristics, as biological compatibility, appropriate marginal sealing, ability to allow or induce bone repair, and ease of manipulation and insertion. Mineral trioxide aggregate (MTA) was developed such a retro-sealer with better chemistry, physics and biological properties than materials that exist at the time, as amalgam, intermediate restorative material (IRM), and SuperEBA. Actually, MTA is used such a canal filling material to seal root apical, at drilling repair, and as a pulp capping agent; in addition, it is used as a filling retrograde material. The purpose of this study was a literature review about mineral trioxide aggregate, comparing it to the main endodontic cements and retro-sealer materials available at market for root canal filling retrograde filling, analyzing its properties and biological characteristics. It was concluded MTA is a quite satisfactory retro-sealer material, in terms of its chemical-physical-biological properties, especially due to its biocompatibility, and potential actions do conduce and induce the repair of bone and cementum.

Avaliação das normas de biossegurança nas clínicas odontológicas da UFPE / Evaluation of biosecutrity norms in UFPE dental clinics

O objetivo deste trabalho foi observar o cumprimento das normas de biossegurança e os cuidados com os riscos ocupacionais pelos alunos que atenderam nas clínicas de Odontologia Preventiva, Odontopediatria, Dentística, Endodontia e nos procedimentos cirúrgicos nas clínicas de Periodontia e Cirurgia da Faculdade de Odontologia da Universidade Federal de Pernambuco. Este trabalho consistiu em um estudo observacional, no qual foram anotados em formulário o cumprimento ou não das normas de biossegurança e cuidados com os riscos ocupacionais pelos alunos durante o atendimento, no período de outubro/2006 a maio/2007. Os alunos foram observados em 09 clínicas, perfazendo no total de 488 fichas observadas. Procurou-se contemplar as clínicas em igual proporção de observações. Os resultados apontaram ausência de manilúvio, inadequações no uso dos EPI para alunos e pacientes, falta de local exclusivo para lavagem de instrumental e deficiências ergonômicas durante a prática clínica. Concluiu-se que é necessária a divulgação mais intensa de políticas públicas que orientem a utilização das normas de biossegurança entre os alunos da instituição, assim como reavaliação e mudanças na infra-estrutura das clínicas e conteúdos dos treinamentos em aula oferecidos aos alunos do curso de Odontologia da UFPE, no intuito de melhor conscientiza-los e prevenir a ocorrência de acidentes ocupacionais.

The purpose of this study was to observe the biosecurity norms execution, as well as the cares with the occupational risky by students who attended at Preventive Dentistry 2, Pediatric Dentistry 1 and 2, Dentistics 2 and 3, Endodontics 2 and 3 clinics, and surgical procedures in Periodontics and Surgery 1 clinics, of Faculdade de Odontologia at Universidade Federal de Pernambuco. This work consisted an observational study, in which were annotated in form the compliance or not of biosecurity norms and caution with occupational risks by the students during the service, in the period from October/2006 to May/2007. Students were observed at 09 clinics, for a total of 488 observed forms. It looked to comtemplate the clinics in equal observations proportion. Results pointed to the washing hands absence, inadequate use of individual protection equipments for students and patients, exclusive place to wash instruments lack and ergonomic deficiencies during the clinical practice. It was concluded it is necessary largest public policies divulgation to guide the biosecurity norms use among institution students, as well as revaluation and changes in clinical infrastructure and class training contents given to UFPE Dentistry students, in order to better awareness them and prevent occupational accidents occurrence.