RESUMO
The SARS-CoV-2 pandemic has affected more than 70 million people worldwide and resulted in over 1.5 million deaths. A broad deployment of effective immunization campaigns to achieve population immunity at global scale will depend on the biological and logistical attributes of the vaccine. Here, two adeno-associated viral (AAV)-based vaccine candidates demonstrate potent immunogenicity in mouse and nonhuman primates following a single injection. Peak neutralizing antibody titers remain sustained at 5 months and are complemented by functional memory T-cells responses. The AAVrh32.33 capsid of the AAVCOVID vaccine is an engineered AAV to which no relevant pre-existing immunity exists in humans. Moreover, the vaccine is stable at room temperature for at least one month and is produced at high yields using established commercial manufacturing processes in the gene therapy industry. Thus, this methodology holds as a very promising single dose, thermostable vaccine platform well-suited to address emerging pathogens on a global scale.
RESUMO
Summary Sediment particles finer than 60m are not uniformly distributed in suspension in waters of the Amazon and Solimões Rivers. Concentrations of such tine particles at the river surface are only half as large as their concentrations in the full water column. Particles as small as 10m are less concentrated near the river surface than at greater depths. Estimates of sediment yield and flux of suspended chemical constituents that are based only on surface samples are likely to contain significant errors.
Resumo Partículas suspensas de sedimentos menores que 60m não estão uniformemente distribuídas nas águas dos rios Amazonas e Solimões. Concentrações destas partículas finas na superfície da água são somente a metade da concentração do que em amostras integradas através de profundidade. Até partículas de 10m estão menos concentradas perto da superfície do que a maiores profundidades. Estimativas de produção de sedimento e fluxo de constituintes químicos cm suspensões que são baseadas somente em amostra da superfície, estão passíveis de conter erros significativamente grandes.
