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1.
Med Oral Patol Oral Cir Bucal ; 29(2): e263-e272, 2024 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-38288853

RESUMO

BACKGROUND: Mandibular third molar (MTM) extraction is one of the most frequently performed surgeries in the oral cavity. Establishing the level of surgical difficulty pre-operatively is an essential step to ensure correct treatment planning. In Spain, MTM extraction - especially in cases presenting greater difficulty - is normally performed by doctors specializing in oral and maxillofacial surgery, or by dentists with postgraduate qualifications in oral surgery. The present work set out to analyze the extent to which perceptions of surgical difficulty of the said intervention vary in relation to professional training. MATERIAL AND METHODS: This cross-sectional, descriptive, observational study took the form of a survey. Using a visual analog scale (VAS), participants evaluated both the perceived difficulty of 30 cases of MTM extraction described by means of digital panoramic radiographs and the perceived difficulty deriving from a series of factors conditioning MTM extraction. The results underwent statistical analysis with SPSS Statistics 28.0 software. Non-parametric tests (Mann Whitney test for independent samples and the Kruskal-Wallis test) were applied. RESULTS: A total of 213 surveys were available for analysis. Both groups awarded the greatest importance to clinical experience, followed by anatomical and radiographic factors, root morphology obtaining the highest score among anatomical factors (9.01±1.42), while proximity of the MTM to the inferior alveolar nerve was regarded as the least important anatomical factor (8.11±2.54). Significant differences were only found for patient age, whereby maxillofacial surgeons awarded this factor more importance than dentists. CONCLUSIONS: The different training received by dentists specialized in oral surgery and maxillofacial surgeons did not influence either perceptions of surgical difficulty of MTM extraction, or opinions as to the factors influencing surgical difficulty.


Assuntos
Cirurgiões Bucomaxilofaciais , Cirurgia Bucal , Humanos , Estudos Transversais , Dente Serotino/cirurgia , Extração Dentária
2.
Med Oral Patol Oral Cir Bucal ; 29(1): e44-e50, 2024 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-37992147

RESUMO

BACKGROUND: Surgical extraction of the lower third molar (LTM) may trigger neurosensory injury of the inferior alveolar nerve, making extraction a real challenge. This study set out to assess whether is it possible to predict neurosensory alterations from preoperative imaging. MATERIAL AND METHODS: A total of 99 patients underwent 124 impacted lower third molar (ILTM) surgeries. Prior to surgery, panoramic and CBCT images were evaluated in an attempt to predict a neurosensory disturbance. Preoperative data (ILTM position, panoramic radiograph signs, inferior alveolar nerve (IAN) location and its contact with the ILTM roots) and intra/postoperative findings (extraction difficulty and sensitivity alterations) were recorded. Descriptive and bivariate data analysis was performed. Statistical comparison applied the chi-square test, Fisher test, and one-way ANOVA test. Statistical significance was established with a confidence interval (CI) of 95%. RESULTS: In 4.03% of cases, patients experienced neurosensory alterations. Of 124 ILTM positions in panoramic radiographs, 76 cases were considered to exhibit a potential neurosensory risk as they presented two or more types of superimposed relationships between ILTM and mandibular canal. Of these, alterations were reported in only three cases (3.95%). Of the 48 remaining ILTM images presenting only one sign, neurosensory alterations were observed in two cases (4.17%). No permanent alterations were recorded in any of the five cases observed. CONCLUSIONS: Within the limitations of the present study, prediction of neurosensory alterations prior to ILTM extraction by means of preoperative imaging did not show a significant statistical correlation with post-surgical incidence. Nevertheless, interruption of the canal´s white line (ICWL) or a diversion of the canal (DC) may predict an increased risk of IAN injury.


Assuntos
Dente Impactado , Traumatismos do Nervo Trigêmeo , Humanos , Dente Serotino/diagnóstico por imagem , Dente Serotino/cirurgia , Estudos Prospectivos , Tomografia Computadorizada de Feixe Cônico , Cuidados Pré-Operatórios , Extração Dentária/efeitos adversos , Dente Impactado/diagnóstico por imagem , Dente Impactado/cirurgia , Dente Impactado/complicações , Radiografia Panorâmica/efeitos adversos , Radiografia Panorâmica/métodos , Nervo Mandibular/diagnóstico por imagem , Traumatismos do Nervo Trigêmeo/etiologia , Mandíbula
3.
Int J Oral Maxillofac Surg ; 51(5): 680-689, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-34507879

RESUMO

This systematic literature review set out to investigate the clinical outcomes of autogenous tooth root blocks used for ridge augmentation: survival rates, block resorption, implant survival, post-surgical complications, and histology findings. This review followed PRISMA guidelines. An automated search was made in four databases, supplemented by a manual search for relevant articles published before December 2020. The quality of evidence provided was assessed with the Newcastle-Ottawa Quality Assessment Scale and the Joanna Briggs Institute Critical Appraisal tool. Seven articles fulfilled the inclusion criteria and underwent analysis. The articles included a total of 136 patients, who received 118 autogenous tooth root blocks and 26 autogenous bone blocks showing block survival rates of 99.15% and 100%, respectively. Tooth root blocks presented a mean bone gain that was similar to autologous bone blocks but showed less resorption. The implant survival rate was 98.32% for autogenous tooth root blocks. Reconstruction of alveolar crests by means of autogenous tooth root blocks appears to be a satisfactory option for single-tooth gaps and low grades of bone atrophy in terms of the survival of the bone block and the implants placed subsequently. More research providing long-term data is needed to confirm these findings.


Assuntos
Aumento do Rebordo Alveolar , Implantes Dentários , Processo Alveolar/cirurgia , Transplante Ósseo , Implantação Dentária Endóssea , Humanos , Raiz Dentária/cirurgia
5.
Br J Oral Maxillofac Surg ; 59(10): 1120-1129, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34503858

RESUMO

This work systematically reviews dental implant placement through impacted teeth or residual roots, as an alternative to invasive extraction surgeries, evaluated in terms of survival rates, marginal bone loss, surgical, and prosthetic complications. The authors conducted an electronic search of four databases up to September 2020; also a complementary handsearch was carried out. The quality of the included studies was assessed using a protocol for assessment of risk of bias in exposure studies. Ten studies fulfilled the inclusion criteria and were analysed. A total of 44 patients received 62 dental implants and were monitored for a minimum of 12-months follow-up. An overall mean implant survival rate was 90.32%, reporting 97.56 % for dental implants through impacted teeth and 76.19% through residual roots. No surgical or prosthetic complications were reported. Placing dental implants through impacted teeth may offer a valid therapeutic option for implant-supported restorations in patients for whom surgery and orthodontic traction are not possible, and/or patients who refuse to undergo more invasive extraction surgery. Moreover, additional caution is recommended when placing implants through retained root fragments, as this may involve long-term risk. Further research generating long-term data are needed to confirm these findings.


Assuntos
Implantes Dentários , Dente Impactado , Implantação Dentária Endóssea , Humanos
6.
Animal ; 15(8): 100301, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34245955

RESUMO

Animal welfare in livestock production is of great interest to consumers. The organic farming approach strives to ensure animal welfare based on preventive measures, but there are very few scientific studies that compare the actual differences in animal welfare between organic and conventional farms. Those studies that have been carried out frequently focus on specific aspects of animal welfare, mostly health issues. The aim of the present study, therefore, was to investigate the effects of the farming system on the welfare of dairy cows in a more holistic way. Although this study was carried out in just two federal states of Germany, the results could serve as a suitable model for the whole country. We used the Welfare Quality assessment protocol to measure welfare for dairy cattle (Welfare Quality®, 2009) and the results showed significant differences (P < 0.05) between organic and conventional farms, but there was also considerable variance between individual farms of the same farming system. Organic farms scored higher in all four Welfare Quality® principles: "Good Feeding", "Good Housing", "Good Health" and "Appropriate Behavior" compared to conventional farms. In particular, organic farms obtained higher scores with respect to Welfare Quality® measures of resting comfort, which contributes to a lower percentage of lameness; organic farms also implemented less painful methods for disbudding, or indeed carried out no disbudding, and provided access to pasture and outdoor exercise. However, organic farms still have room for improvement, especially with respect to animal health. Therefore, outcome-based specifications should be included in the current (purely action-oriented) European regulation of organic production (EC, 2008; EU, 2018) to safeguard the health-related aspects of animal welfare.


Assuntos
Indústria de Laticínios , Abrigo para Animais , Agricultura , Bem-Estar do Animal , Animais , Bovinos , Fazendas , Feminino
7.
Med. oral patol. oral cir. bucal (Internet) ; 25(2): e291-e298, mar. 2020. tab, graf
Artigo em Inglês | IBECS | ID: ibc-196260

RESUMO

BACKGROUND: This systematic literature review aimed to evaluate the efficacy of allogeneic bone blocks for ridge augmentation by assessing block survival rates and subsequent implant survival, including post-surgical complications and histomorphometric analysis. MATERIAL AND METHODS: An electronic and manual search among references, was conducted up to April 2019 by two independent authors. Inclusion criteria were: human clinical trials in which the outcomes of allogeneic bone block grafts were evaluated by means of their survival rates and subsequent implant success rates. RESULTS: Seven articles fulfilled the inclusion criteria and were analyzed. A total of 323 allogeneic block grafts were monitored for a minimum of 12 months follow-up after surgery, of which thirteen (4.02%) failed. Regarding the cumulative implant survival rate, the weighted mean was 97.36%, computed from 501 implants. Histologic and histomorphometric analysis showed that allogeneic block grafts presented some clinical and microstructural differences in comparison with autologous block grafts. CONCLUSIONS: Atrophic alveolar crest reconstruction with allogeneic bone block grafts would appear a feasible alternative to autologous bone block grafts, obtaining a low block graft failure rate, similar implant survival rate and fewer postoperative complications. Further investigations generating long term data are needed to confirm these findings


No disponible


Assuntos
Humanos , Masculino , Feminino , Aumento do Rebordo Alveolar/métodos , Transplante Ósseo/métodos , Transplante Homólogo/métodos , Complicações Pós-Operatórias , Resultado do Tratamento , Viés
8.
Med Oral Patol Oral Cir Bucal ; 25(2): e291-e298, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32040468

RESUMO

BACKGROUND: This systematic literature review aimed to evaluate the efficacy of allogeneic bone blocks for ridge augmentation by assessing block survival rates and subsequent implant survival, including post-surgical complications and histomorphometric analysis. MATERIAL AND METHODS: An electronic and manual search among references, was conducted up to April 2019 by two independent authors. Inclusion criteria were: human clinical trials in which the outcomes of allogeneic bone block grafts were evaluated by means of their survival rates and subsequent implant success rates. RESULTS: Seven articles fulfilled the inclusion criteria and were analyzed. A total of 323 allogeneic block grafts were monitored for a minimum of 12 months follow-up after surgery, of which thirteen (4.02%) failed. Regarding the cumulative implant survival rate, the weighted mean was 97.36%, computed from 501 implants. Histologic and histomorphometric analysis showed that allogeneic block grafts presented some clinical and microstructural differences in comparison with autologous block grafts. CONCLUSIONS: Atrophic alveolar crest reconstruction with allogeneic bone block grafts would appear a feasible alternative to autologous bone block grafts, obtaining a low block graft failure rate, similar implant survival rate and fewer postoperative complications. Further investigations generating long term data are needed to confirm these findings.


Assuntos
Aumento do Rebordo Alveolar , Implantes Dentários , Transplante de Células-Tronco Hematopoéticas , Atrofia , Transplante Ósseo , Implantação Dentária Endóssea , Humanos
9.
J Stomatol Oral Maxillofac Surg ; 121(4): 390-396, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31904530

RESUMO

INTRODUCTION: Surgical inferior alveolar nerve (IAN) reposition techniques offer an alternative approach to implant-based rehabilitation in patients with severe mandibular atrophy The aim of this systematic review, was to determine the complications associated with the technique and to determine which of two variants (lateralization or transposition) is less invasive. MATERIALS AND METHODS: An electronic search was conducted in databases complimented by a manual search to identify clinical studies investigating complications derived from these surgical techniques. Only studies of adult humans, published in English during the last seven years were included. The initial search located 78 articles, of which seven were included in analysis on the basis of the following characteristics: four investigated inferior alveolar nerve lateralization (IANL), one inferior alveolar nerve transposition (IANT), and two investigated both reposition techniques. RESULTS: This review included data from 289 patients who were recruited for lateralization (N=319) or transposition surgery (N=33) making a total of 352 reposition procedures. Five patients (1.73%) suffered persistent damage to the IAN at the end of the follow-up periods. The overall implant survival rate was 99.26% of a total of 817 implants. The most common complications were neurosensory problems, mandibular fracture, infection, implant loss, and insufficient anatomical reconstruction of the atrophic mandible; neurosensory complications (hypoesthesia, paraesthesia, and hyperesthesia caused by traumatic damage to the nerve) were the most prevalent. CONCLUSIONS: Lateralization of the inferior alveolar nerve would appear to be less invasive as it produces lower percentages of persistent neurosensory disorders (1.56%) than transposition (12.12%). Nevertheless, both techniques offer a viable approach to implant placement in edentulous atrophic mandibles, obtaining predictable clinical and radiological results after 5 years implant loading.


Assuntos
Implantação Dentária Endóssea , Implantes Dentários , Adulto , Atrofia/patologia , Implantação Dentária Endóssea/efeitos adversos , Implantes Dentários/efeitos adversos , Humanos , Mandíbula/patologia , Mandíbula/cirurgia , Nervo Mandibular/cirurgia
10.
Med Oral Patol Oral Cir Bucal ; 24(5): e615-e620, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31422412

RESUMO

BACKGROUND: To establish the prevalence of supernumerary canines (SNC) in a sector of the population of Madrid (Spain), as well possible complications associated with this unusual developmental variation. STUDY DESIGN: This observational study was performed between 2005 and 2017, among 21,615 patients seeking dental treatment at the Faculty of Dentistry, Complutense University of Madrid (Spain), and at the Virgen de la Paloma Hospital, Madrid (Spain); 22 patients with 26 SNCs were diagnosed. These 22 patients underwent clinical and radiological exploration, registering patient data. RESULTS: SNCs presented a prevalence of 0.10% of the study population. The supernumerary teeth (SNT) were located in the upper maxilla more frequently (61.54%) than the mandible (38.46%). 69.23% were found to be impacted, also causing the impaction of the permanent canine in 53.85% of these cases. In 15.38%, follicular expansion > 3mm was observed. SNCs were associated with other SNT in only four patients. CONCLUSIONS: Despite of the fact that the SNCs are usually diagnosed casually in the course of radiological exploration, in the present study over half of them (53.85%) caused impaction of the permanent canine. Early diagnosis allows optimal patient management and treatment planning, with intervention at an appropriate time to prevent complications in development and so reduce later treatment need.


Assuntos
Dente Impactado , Dente Supranumerário , Dente Canino , Humanos , Mandíbula , Maxila , Espanha
11.
Med Oral Patol Oral Cir Bucal ; 23(1): e92-e97, 2018 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-29274163

RESUMO

BACKGROUND: The main clinical application of electromyography is to detect abnormalities in muscle function, to assess muscle activity for purposes of recruitment, and in the biomechanics of movement. OBJECTIVES: To analyze electromyography (EMG) findings for masticatory muscles during chewing following surgical extraction of lower third molars, and to determine any correlation between pain, inflammation, trismus, and the EMG data registered. MATERIAL AND METHODS: This prospective study included 31 patients. Surface EMG was used to study masseter and temporalis muscle function before lower third molar extraction and 72 hours and seven days after surgery. Clinical variables, pain, inflammation, and trismus were registered before and after surgery. RESULTS: Studying the area and size of the masticatory muscles, higher values were found for temporalis than masseter muscles, regardless of the surgical side, which points to the greater involvement of the temporalis muscle in mastication. Comparing the side where surgery had been performed with the non-surgical side, a sharp and statistically significant reduction in amplitude and area were noted on the surgical side reflecting major functional affectation. One week after surgery, amplitude and area had almost returned to base-line values, indicating almost complete recovery. While pain decreased progressively after surgery, inflammation peaked at 72 hours, while mouth opening reached a minimum at this time, returning to normality within the week. CONCLUSIONS: Surgical extraction of lower third molars produces changes to electromyography activity that are more evident during the first hours after surgery and closely related to the intensity of pain suffered and the patient's inflammatory responses, although they are not related to mouth opening capacity.


Assuntos
Eletromiografia , Músculo Masseter/fisiologia , Dente Serotino/cirurgia , Músculo Temporal/fisiologia , Extração Dentária , Feminino , Humanos , Inflamação/diagnóstico , Inflamação/fisiopatologia , Estudos Longitudinais , Masculino , Mandíbula , Mastigação , Dor/fisiopatologia , Cuidados Pós-Operatórios , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/fisiopatologia , Cuidados Pré-Operatórios , Estudos Prospectivos , Trismo/diagnóstico , Trismo/fisiopatologia , Adulto Jovem
12.
Oral Implantol (Rome) ; 10(4): 448-456, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29682262

RESUMO

OBJECTIVE: The objective is to present a clinical case of a 38-year-old male with a maxillary unicystic ameloblastoma treated by means of tumor block resection followed by chin-harvested graft placement in order to place two dental implants for esthetic and functional rehabilitation. METHODS: Ameloblastoma is a benign odontogenic tumor characterized by local aggression and a high rate of recurrence; the latter partly depends on how it is treated. Complete resection of the tumor, which usually prevents recurrence, produces bone defects of varying size that must be reconstructed later on. In most cases this is done using bone grafts and implant-supported prostheses. Grafts harvested from the chin are relatively easy to obtain and enjoy a fairly uneventful post-operative with few complications; they are suitable for cases in which the defect generated by resection is of small size. RESULTS: Functional and esthetic rehabilitation and the tumor has not relapsed during a 7-year follow-up. CONCLUSION: Tumor block resection followed by chin-harvested graft placement and dental implants is a safe treatment for patients with unicystic ameloblastoma.

13.
J Dairy Sci ; 99(7): 5486-5497, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27108172

RESUMO

The casein genes are known to be highly variable in typical dairy species, such as cattle and goat, but the knowledge about equine casein genes is limited. Nevertheless, mare milk production and consumption is gaining importance because of its high nutritive value, use in naturopathy, and hypoallergenic properties with respect to cow milk protein allergies. In the current study, the open reading frames of the 4 casein genes CSN1S1 (αS1-casein), CSN2 (ß-casein), CSN1S2 (αS2-casein), and CSN3 (κ-casein) were resequenced in 253 horses of 14 breeds. The analysis revealed 21 nonsynonymous nucleotide exchanges, as well as 11 synonymous nucleotide exchanges, leading to a total of 31 putative protein isoforms predicted at the DNA level, 26 of which considered novel. Although the majority of the alleles need to be confirmed at the transcript and protein level, a preliminary nomenclature was established for the equine casein alleles.


Assuntos
Caseínas/genética , Variação Genética , Cavalos/genética , Alelos , Animais , Cruzamento , Caseínas/análise , DNA , Feminino , Leite/química , Fases de Leitura Aberta/genética
14.
Int J Oral Maxillofac Surg ; 45(7): 858-63, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26837718

RESUMO

The objectives of this study were to evaluate the survival after 5 years of implants placed using inferior alveolar nerve (IAN) lateralization in cases of mandibular atrophy and to determine the incidence of complications. Twenty-seven patients received 74 implants by means of the IAN lateralization technique. Implant survival after 5 years of loading was 98.6%. Eighteen months after surgery, the recovery of sensitivity was complete in 26 cases. Implant placement with IAN lateralization was seen to be a satisfactory and predictable technique. IAN lateralization requires a high level of technical skill, and strict criteria should be applied when prescribing this treatment.


Assuntos
Implantação Dentária Endóssea/métodos , Falha de Restauração Dentária/estatística & dados numéricos , Mandíbula/inervação , Mandíbula/patologia , Nervo Mandibular/cirurgia , Complicações Pós-Operatórias/epidemiologia , Perda do Osso Alveolar , Implantação Dentária Endóssea/efeitos adversos , Implantes Dentários , Prótese Dentária Fixada por Implante , Seguimentos , Humanos , Incidência , Estudos Prospectivos , Recuperação de Função Fisiológica , Sensação , Fatores de Tempo , Resultado do Tratamento
15.
Animal ; 10(2): 342-8, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26144555

RESUMO

In this study, a data set of 2922 lactating dairy cows in a sample of 64 conventional and organic dairy farms with Holstein Friesian cows in Germany and 31 conventional dairy farms with the dual purpose breed Fleckvieh in Austria was used to screen for correlations between the occurrences of different integument alterations. All cows were housed in cubicle systems. Alterations were classified as hairless areas (H), scabs or wounds (W) or swellings (S) and assessed at 15 locations of the cows' body. Highest median farm prevalences were found at the joints of the legs, which are already commonly included in studies on integumentary alterations: median farm prevalence was 83% for S and 48% for H at the carpal joints, followed by H (38%) and S (20%) at the lateral tarsal joints and H at the lateral calcanei (20%). Additional body parts with notable median prevalences for H were the hip bones (13%), pin bones (12%) and sacrum (11%). Three cluster models, with 2, 5 and 14 clusters, were built by hierarchical clustering of prevalences of the 30 most relevant alteration location combinations. Clustering revealed that location overruled type of lesion in most cases. Occasionally, clusters represented body segments significantly distant from each other, for example the carpal joints and lateral and dorsal calcanei. However, some neighbouring areas such as the medial and lateral hock area should be analysed separately from each other for causal analysis as they formed distinct clusters.


Assuntos
Bovinos/lesões , Bovinos/fisiologia , Abrigo para Animais/classificação , Pele/lesões , Ferimentos e Lesões/veterinária , Bem-Estar do Animal , Animais , Áustria , Cruzamento , Carpo Animal/lesões , Carpo Animal/patologia , Análise por Conglomerados , Indústria de Laticínios , Feminino , Alemanha , Abrigo para Animais/normas , Lactação , Prevalência , Pele/patologia , Tarso Animal/lesões , Tarso Animal/patologia , Ferimentos e Lesões/epidemiologia , Ferimentos e Lesões/patologia
16.
Cell Death Dis ; 3: e388, 2012 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-22951985

RESUMO

In this study, we have analysed the apoptotic effects of the ubiquitous environmental toxin benzo[a]pyrene (BP) in HaCaT cells and human keratinocytes. Although prolonged exposure to BP was not cytotoxic on its own, a strong enhancement of CD95 (Fas)-mediated apoptosis was observed with BP at concentrations activating the aryl hydrocarbon receptor (AhR). Importantly, the ultimately mutagenic BP-metabolite, that is, (+)-anti-BP-7,8-diol-9,10-epoxide (BPDE), failed to enhance CD95-mediated cell death, suggesting that the observed pro-apoptotic effect of BP is neither associated with DNA adducts nor DNA-damage related signalling. CD95-induced apoptosis was also enhanced by ß-naphtoflavone, a well-known agonist of the AhR that does not induce DNA damage, thus suggesting a crucial role for AhR activation. Consistently, BP failed to sensitise for CD95L-induced apoptosis in AhR knockdown HaCaT cells. Furthermore, inhibition of CYP1A1 and/or 1B1 expression did not affect the pro-apoptotic crosstalk. Exposure to BP did not increase expression of CD95, but led to augmented activation of caspase-8. Enhancement of apoptosis was also observed with the TRAIL death receptors that activate caspase-8 and apoptosis by similar mechanisms as CD95. Together, these observations indicate an interference of AhR signalling with the activity of receptor-associated signalling intermediates that are shared by CD95 and TRAIL receptors. Our data thus suggest that AhR agonists can enhance cytokine-mediated adversity upon dermal exposure.


Assuntos
Apoptose/efeitos dos fármacos , Benzo(a)pireno/toxicidade , Proteína Ligante Fas/metabolismo , Receptores de Hidrocarboneto Arílico/metabolismo , Receptores do Ligante Indutor de Apoptose Relacionado a TNF/metabolismo , 7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido/farmacologia , Hidrocarboneto de Aril Hidroxilases/antagonistas & inibidores , Hidrocarboneto de Aril Hidroxilases/metabolismo , Benzo(a)pireno/metabolismo , Caspase 8/metabolismo , Linhagem Celular , Citocromo P-450 CYP1A1/antagonistas & inibidores , Citocromo P-450 CYP1A1/metabolismo , Citocromo P-450 CYP1B1 , Adutos de DNA/química , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Receptores de Hidrocarboneto Arílico/agonistas , Receptores de Hidrocarboneto Arílico/antagonistas & inibidores , Transdução de Sinais , beta-Naftoflavona/farmacologia
17.
J Anim Sci ; 89(11): 3809-18, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21571890

RESUMO

The effects of floor space on the trailer and journey time during transport from the farm to the packing plant on indicators of stress (open-mouth breathing, muscle tremors, and skin discoloration) and on the incidence of transport losses (dead on arrival, nonambulatory, noninjured, and nonambulatory, injured) were evaluated in a study involving 160 loads of market-weight pigs (BW 124.7 ± 4.38 kg) using a split-plot design with a 2 × 6 factorial arrangement of treatments: 1) journey time [main plot; short (<1 h) and long (3 h)] and 2) floor space (subplot; 0.396, 0.415, 0.437, 0.462, 0.489, and 0.520 m(2)/pig, which is equivalent to 0.317, 0.332, 0.350, 0.370, 0.391, and 0.416 m(2)/100 kg of BW, respectively). Two consecutively loaded trailers were randomly allotted to journey time treatment. Floor space treatments were compared in the front 3 compartments on the top and bottom decks of the trailer and were created by varying the number of pigs per compartment, which confounds the effect of floor space with group size. Of the 17,652 pigs transported in 954 test compartments, 0.24% died or became nonambulatory. Neither journey time nor floor space had an effect (P > 0.05) on the incidence of dead and nonambulatory, injured pigs, or on total transport losses. There were interactions (P < 0.05) between journey time and floor space treatments for the incidences of nonambulatory, noninjured pigs and open-mouth breathing. For 2 of the smallest floor spaces (0.415 and 0.437 m(2)/pig), the incidence of nonambulatory, noninjured pigs was greater on short than on long journeys; for the other 4 floor spaces there was no effect (P > 0.05) of journey time. The incidence of open-mouth breathing for the 3 smallest floor spaces was greater (P < 0.05) for short than long journeys, whereas there was no effect (P > 0.05) of journey time for the 3 greatest floor spaces. The frequency of skin discoloration was greater (P < 0.001) for pigs transported at the 2 smallest floor spaces compared with the other 4 floor spaces. In summary, short journey time increased the frequency of indicators of stress after unloading at the plant for pigs transported at smaller floor spaces and also increased the incidence of nonambulatory, noninjured pigs at 2 of the 3 smallest floor spaces. However, neither transport floor space nor journey time had an effect on total losses.


Assuntos
Bem-Estar do Animal , Estresse Fisiológico/fisiologia , Suínos/fisiologia , Meios de Transporte , Animais , Distribuição de Qui-Quadrado , Feminino , Masculino , Distribuição Aleatória
18.
J Dairy Sci ; 92(11): 5476-86, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19841210

RESUMO

Lameness poses a considerable problem in modern dairy farming. Several new developments (e.g., herd health plans) strive to help farmers improve the health and welfare of their herd. It was thus our aim to identify lameness risk factors common across regions, breeds, and farming systems for freestall-housed dairy cows. We analyzed data from 103 nonorganic and organic dairy farms in Germany and Austria that kept 24 to 145 Holstein Friesian or Fleckvieh cows in the milking herd (mean = 48). Data on housing, management, behavior, and lameness scores for a total of 3,514 cows were collected through direct observations and an interview. Mean lameness prevalence was 34% (range = 0-81%). Data were analyzed applying logistic regression with generalized estimating equations in a split-sample design. The final model contained 1 animal-based parameter and 3 risk factors related to lying as well as 1 nutritional animal-based parameter, while correcting for the significant confounders parity and data subset. Risk for lameness increased with decreasing lying comfort, that is, more frequent abnormal lying behavior, mats or mattresses used as a stall base compared with deep-bedded stall bases, the presence of head lunge impediments, or neck rail-curb diagonals that were too short. Cows in the lowest body condition quartile (1.25-2.50 for Holstein Friesian and 2.50-3.50 for Fleckvieh) had the highest risk of being lame. In cross-validation the model correctly classified 71 and 70% of observations in the model-building and validation samples, respectively. Only 2 out of 15 significant odds ratios (including contrasts) changed direction. They pertained to the 2 variables with the highest P-values in the model. In conclusion, lying comfort and nutrition are key risk areas for lameness in freestall-housed dairy cows. Abnormal lying behavior in particular proved to be a good predictor of lameness risk and should thus be included in on-farm protocols. The study is part of the European Commission's Welfare Quality project.


Assuntos
Criação de Animais Domésticos/métodos , Cruzamento , Doenças dos Bovinos/epidemiologia , Abrigo para Animais/normas , Coxeadura Animal/epidemiologia , Animais , Áustria , Bovinos , Doenças dos Bovinos/prevenção & controle , Feminino , Alemanha , Coxeadura Animal/prevenção & controle , Reprodutibilidade dos Testes , Fatores de Risco
19.
J Anim Sci ; 86(11): 3137-45, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18567725

RESUMO

This study evaluated effects of trailer design and season on physical indicators of stress during loading and unloading and transport losses (dead and nonambulatory pigs) in market-weight pigs (BW = 129.6 +/- 0.40 kg). A total of 109 trailer loads of pigs (n = 17,256 pigs) from 1 farm were used in a randomized complete block design with a 2 x 4 factorial arrangement of treatments: 1) trailer design (potbelly vs. straight-deck) and 2) season (spring vs. summer vs. fall vs. winter). A subset of loads (n = 42) was used to examine effect of distance pigs were moved during loading [short (<24 m) vs. long (47 to 67 m)] on physical indicators of stress and transport losses. This study was conducted on 7 d per season at 1 farm with 4 loads (2 on potbelly and 2 on straight-deck trailers) being transported each day to 1 commercial packing plant. Pigs from different farm groups were mixed on the trailer and provided with 0.45 m(2)/pig floor space during an approximately 4-h journey to the plant. The percentage of pigs exhibiting open-mouth breathing, skin discoloration, and muscle tremors was recorded during loading and unloading. Additionally, dead pigs on arrival at the plant and nonambulatory pigs at the farm and at the plant were recorded. Effects of trailer design on open-mouth breathing and skin discoloration during unloading were dependent on season (trailer design x season interaction; P < 0.05). Pigs unloaded from potbelly trailers had a greater (P < or = 0.05) incidence of open-mouth breathing in the spring and summer and a greater (P < 0.05) incidence of skin discoloration in the spring, summer, and winter than pigs unloaded from straight-deck trailers. The incidence of total nonambulatory pigs at the plant was greater (P < 0.05) in the winter than in the spring and summer. The long compared with short distance moved treatment resulted in a greater (P = 0.001) incidence of open-mouth breathing and skin discoloration during loading and tended (P = 0.06) to increase the incidence of nonambulatory pigs at the farm. However, there was no effect of trailer design, season, or loading distance on total losses at the plant. In summary, physical indicators of stress (open-mouth breathing and skin discoloration) were increased with the long distance moved during loading treatment and were greater during unloading for potbelly than straight-deck trailers; however, trailer design, season, and loading distance had minimal effects on total transport losses.


Assuntos
Criação de Animais Domésticos/instrumentação , Veículos Automotores/normas , Estações do Ano , Suínos/fisiologia , Meios de Transporte , Criação de Animais Domésticos/métodos , Criação de Animais Domésticos/normas , Animais , Composição Corporal/fisiologia , Feminino , Masculino , Mortalidade , Estresse Fisiológico , Fatores de Tempo , Meios de Transporte/normas
20.
J Anim Sci ; 85(12): 3454-61, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17785596

RESUMO

Effects of distance moved during loading and floor space on the trailer during transport on the incidence of transport losses (dead and nonambulatory pigs) on arrival at the packing plant were evaluated in a study involving 42 loads of pigs (average BW = 131.2 kg, SD 5.05). A split-plot design was used with a 2 x 6 factorial arrangement of the following treatments: 1) distance moved from the pen to the exit of the building [short (0 to 30.5 m) vs. long (61.0 to 91.4 m)] and 2) transport floor space (0.396, 0.415, 0.437, 0.462, 0.489, or 0.520 m(2)/pig). Loading distance treatments (sub-plots) were compared within transport floor space treatments (main plot). Pigs were loaded at the farm using sorting boards and, if necessary, electric goads, transported approximately 3 h to a commercial packing plant and unloaded using livestock paddles. The number of nonambulatory pigs during loading and the number of dead and nonambulatory pigs at the plant were recorded. Nonambulatory pigs were classified as fatigued, injured, or injured and fatigued. In addition, the incidence of pigs exhibiting signs of stress (open-mouth breathing, skin discoloration, and muscle tremors) during loading and unloading was recorded. There were no interactions (P > 0.05) between distance moved and transport floor space treatments. Moving pigs long compared with short distances during loading increased (P < 0.001) the incidence of open-mouth breathing after loading (24.9 vs. 11.0 +/- 1.03%, respectively) and tended to increase the incidence of nonambulatory pigs during loading (0.32 vs. 0.08 +/- 0.09%, respectively; P = 0.09) and of nonambulatory, injured pigs at the plant (0.24 vs. 0.04 +/- 0.07%, respectively; P = 0.06). However, distance moved did not affect other losses at the plant. Total losses at the plant were greater (P < 0.05) for the 3 lowest floor spaces compared with the 2 highest floor spaces, and pigs provided 0.462 m(2)/pig during transport had similar transport losses to those provided 0.489 and 0.520 m(2)/pig (total losses at the plant = 2.84, 1.88, 1.87, 0.98, 0.13, and 0.98 +/- 0.43% of pigs transported, for 0.396, 0.415, 0.437, 0.462, 0.489, and 0.520 m(2)/pig, respectively). These data confirm previous findings that transport floor space has a major effect on transport losses and suggest that these losses are minimized at a floor space of 0.462 m(2)/pig or greater.


Assuntos
Criação de Animais Domésticos/métodos , Pisos e Cobertura de Pisos , Suínos/lesões , Suínos/fisiologia , Meios de Transporte , Bem-Estar do Animal , Animais , Peso Corporal , Feminino , Masculino , Densidade Demográfica , Distribuição Aleatória , Suínos/psicologia , Fatores de Tempo
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