Characterization and differentiation of Lactobacillus manihotivorans strains isolated from cassava sour starch.

Lactobacillus manihotivorans has been reported as one of the dominant species in cassava sour starch production process. Seven isolates that have previously been identified as belonging to this species were studied in the present work. Their molecular and phenotypic characteristics showed higher strain diversity than previously described. Differences were found in their fermentation profiles, whereas no major differences were observed in properties related to processing conditions (salt concentration, pH, temperature), or in potential functional properties (bile salt and pH 2.0 tolerance). Among the main characteristics of interest for the fermentation of cereals or cassava, blended or not with legumes, six out of seven strains were amylolytic and raffinose was fermented by all strains. Strains OND 32T and OLB 7 fermented the broadest range of carbohydrates. Most of the strains contained plasmids. Plasmid curing changed their phenotypic characteristics, particularly those of strain OND 32T, which, in addition, lost its starch and raffinose fermentation ability.

[Basidiomycetes: A new source of secondary metabolites.]. / Basidiomicetos: nueva fuente de metabolitos secundarios.

The area of natural products research is the most rapidly growing field of organic chemistry, due to the great technical developments in the isolation and identification techniques. Today, near to one million natural products -isolated from the most diverse living things- are known. Microorganisms are among the least-studied of these. Nevertheless, they offer large possibilities for the discovery of new structures and biological activities. Among the microorganisms, the Basidiomycetes present a production capacity and a range of biologically active metabolites, which have scarcely been investigated. The wide spectrum of natural products with biological activity produced by Basidiomycetes includes antimicrobial agents, antifungal, antiviral and cytotoxic activities, enzymes, plant growth regulators and flavors. These metabolites are generally grouped by their chemical origin, and the relationship between chemical structure and the different biological activities reported. The main objective of this review is to bring an updated revision of the numerous and interesting biosynthetic pathways from basidiomycetes.

Identification of a laccase gene family in the new lignin-degrading basidiomycete CECT 20197.

A new lignin-degrading basidiomycete, strain I-62 (CECT 20197), isolated from decayed wood exhibited both a high dephenolization activity and decolorization capacity when tested on effluents from the sugar cane by-product fermentation industry. It has been classified as a member of the Polyporaceae family. The major ligninolytic activity detected in culture supernatants of basidiomycete I-62 was a phenoloxidase (laccase), in conjunction with small amounts of manganese peroxidase. No lignin peroxidase was detected. Laccase activity was produced in either defined or complete media. Addition of veratryl alcohol as the inducer, in defined medium, enhanced laccase production 10-fold. The use of fructose instead of glucose as a carbon source resulted in a 100-fold increase in laccase specific activity. Native isoelectrofocusing gels stained with guaiacol revealed the presence of at least seven laccase isozymes, with the most intense band being detected at pI 3. Southern hybridization analysis indicated the presence of a laccase gene family in strain I-62. Three different genes coding for phenoloxidases, lcc1, lcc2, and lcc3, were cloned and characterized. The high degree of homology between laccases from strain I-62 and laccases from Trametes species suggests a phylogenetic proximity between this new isolated fungus and the genus Trametes.

Patterns in grass silicification: response to grazing history and defoliation.

Morphologically distinct populations of a North American perennial grass, Agropyron smithii, collected from a heavily grazed prairie dog (Cynomys ludovicianus) colony (PDC) and a grazing exclosure (EX), were grown in an environmental chamber to determine whether: (1) leaf silicon (Si) concentrations are greater in plant populations which differentiated under heavy grazing pressure, and (2) leaf silicification is inducible by defoliation. Mean shoot Si concentration of nondefoliated plants was greater in the PDC population (2.2%) than the EX population (1.9%) over the 18 wk experiment, largely as a result of differences in Si concentrations in leaf blades. However, leaf Si concentration was lower in defoliated plants of each population than in nondefoliated plants, indicating that leaf silicification was not an inducible herbivore defense mechanism in A. smithii. The higher leaf Si concentrations from the heavily grazed population may be associated with grazingrelated environmental stresses such as a warmer, drier microclimate or with morphological characteristics related to grazing tolerance or avoidance.