A methodological approach to compare continuous and instantaneous sampling and two methods to deal with animals out of sight on dairy cattle behavior and interaction with their calf in the first hours post-partum.

Animal behavior can provide useful information about animal welfare, but methods and tools used to gather behavioral data and data treatment can influence the results. Therefore, this study was carried out on dairy cow (Bos taurus) behavior and interaction with calves early post-partum aiming at comparing two sampling rules, namely continuous and instantaneous sampling at scan intervals of 30 s, 1, 2, 3, 4, 5, and 10 min, and of two methods to deal with out of sight animals. The study was based on three assumptions: (1) continuous sampling provides the most complete and accurate data, allowing the observation of seldom behaviors and short events; (2) instantaneous sampling can provide accurate measurements of frequency and duration, especially at short scan intervals; (3) differences in behavioral results may occur depending on whether a correction for time out of sight is applied or not. Thus, 10 dams were observed from videos in the 2 h post-parturition. Ruminating, stereotypies, calf-biting and calf-butting were not recorded during the observation period. Other behaviors were observed only with continuous sampling or with continuous and instantaneous at 30-s scan intervals. The recoding of several behaviors was less accurate when applying longer scan intervals. Data from continuous and instantaneous sampling at 30-s scan intervals were compared with Wilcoxon test. Results showed no significant differences for posture, position in the pen and all behaviors (p > 0.05) except vocalizing (p = 0.003). The same test did not highlight significant differences due to method of dealing with out of sight for both sampling rules (p > 0.05). Correlation between continuous and instantaneous sampling were prevalently high at 30-s intervals and they decreased as the length of scan intervals increased for most behaviors. Results confirmed the first two assumptions suggesting that continuous sampling is more accurate, in particular for short and rare behaviors, and caution against the suitability of dam behavioral data collected using instantaneous sampling at scan intervals of minutes. The third assumption was not proven by this study. Results should be considered in light of the development of new technologies that relies on data acquired by sensors and imaging to monitor cow-calf welfare and behavior post-parturition.

Exploring the Effectiveness of Problem-Based Learning in an International Undergraduate Program in Veterinary Sciences: Students' Satisfaction, Experience and Learning.

The systematic evaluation of the integration of problem-based learning (PBL) into educational programs in Italy is scarce and there are no published reports of its use in an Italian Bachelor of Veterinary Science degree program. This paper aims to assess the satisfaction of second-year students on an international Bachelor of Veterinary Science degree program after implementing two weeks of PBL with a multidisciplinary approach. Moreover, the impact of this methodological approach on the students' performance and their perceptions concerning their learning experience was investigated. The results showed that students expressed a high level of satisfaction and a positive attitude towards learning through PBL. A significant increase in the perception of students' soft skills was also found, based on self-evaluation. Moreover, a significant improvement was seen in the students' perception of their learning and teaching experiences and general life competencies, assessed using the validated questionnaire HowULearn. Negative effects were also identified, requiring further design modification of the tutors' feedback and pedagogical orchestration. Based on our findings, when planning bachelor's degree programs in veterinary science, PBL modules or activities should be considered to promote active learning, engagement among students, and the improvement of problem-solving and team-working skills.

A retrospective study on the prevalence of main clinical findings in brown bears (Ursus arctos) rescued from substandard husbandry conditions.

Brown bears (Ursus arctos) are kept under varied captive conditions, some of which may greatly compromise their welfare. FOUR PAWS is an NGO that rescues some of these bears kept in substandard conditions and houses them in species-appropriate sanctuaries, where preventive and reactive veterinary care is provided. This retrospective study aims to provide an overview of pathologies and clinical abnormalities reported in veterinary records and their prevalence according to body system affected and pre-rescue bear origin. Origin was categorised as subzoo (bears coming from substandard zoos), dancing (used to "dance" upon a music cue), restaurant (used to attract clients), private keeping (used for various purposes, such as photo props), circus (used for shows), and bear-baiting (exploited for hunting dog training in baiting stations). Clinical findings were extracted from reports of veterinary examinations done from 2006 to 2021, during rescue, routinely, in response to clinical signs, and/or post-mortem. Their prevalence was calculated according to the body system affected and neoplasia (specific group independent from the organ) over the findings' total number. Prevalence was also calculated according to pre-rescue origin (general and relative values in proportion to the number of reports per origin). Results refer to 302 veterinary reports of 114 bears examined, rescued from 1998 to 2021, with the age at rescue varying from a few months to 30 years (median 13 years). The total number of clinical findings was 1,003, and the systems with more findings were oral cavity (56.0%), abdominal cavity and digestive system (7.9%), integumentary (7.9%), ocular systems (7.7%), and musculoskeletal (7.6%). Findings involving other body systems and neoplasia were less prevalent (≤2.8%). Results showed a higher prevalence of some clinical findings for bears rescued from certain origins compared to others. Straightforward associations between pre-rescue origin and clinical findings were not feasible due to unknown anamnesis and details on pre-rescue conditions, and because some housing and management characteristics might be transversal to origins. Results suggest that bears rescued from certain origins were prone to specific clinical findings, supporting the need for the creation of ad hoc preventive veterinary and husbandry management plans after rescue, thus contributing to the improvement of captive bear welfare.

Challenging suicide, burnout, and depression among veterinary practitioners and students: text mining and topics modelling analysis of the scientific literature.

BACKGROUND: Worldwide, veterinary practitioners and students are reported to be at higher risk of suicide, burnout, and depression compared to other occupational groups. The aim of the current study was to apply text mining and topic modelling analysis on scientific literature regarding suicide, burnout, and depression among veterinary practitioners and students to extract meaningful and synthetic information. These statistical approaches can be used to comprehend more in deep the phenomena involving veterinarians and veterinary students and to suggest the potential changes needed in admission to veterinary school, veterinary curricula, and post-graduation initiatives as preventive actions. RESULTS: A systematic search protocol was set up to identify scientific literature that published on the topic from 1985 to 2019. Two-hundred-eleven records were selected with abstracts/texts submitted to text mining and topic modelling analysis. Student, stress, work, anim*, and euthanasia resulted the most frequent terms. Topics modelling allowed to differentiate groups of words and papers in 3 areas of interest: 1) students' difficulties encountered during their studies that increase stress and anxiety impairing their psychological health; 2) exposure to death and euthanasia as risk factor for mental health; and 3) need of support among those providing medical and health care, and of supportive group work to cope with such profession. CONCLUSION: Based on the most frequent words included in the clouds and on the contents of the papers clusterised in them, some suggestions are interfered. It is emphasized that the veterinary curricula should include courses that prepare them early to deal with animal death and post-death grief of pet owners, to handle ethical dilemmas and moral stressors, to communicate with clients and staff members, to work in team, to balance work-family life and to promote individual and team resources. Specific courses for veterinary practitioners could keep them updated on their new roles and ways to handle them among functioning as potential feedbacks to monitor their psychological wellbeing.

The Use of the General Animal-Based Measures Codified Terms in the Scientific Literature on Farm Animal Welfare.

Background: The approach to farm animal welfare evaluation has changed and animal-based measures (ABM), defined as the responses of an animal or effects on an animal, were introduced to assess animal welfare. Animal-based measures can be taken directly on the animal or indirectly and include the use of animal records. They can result from a specific event or be the cumulative outcome of many days, weeks, or months. The objective of the current study was to analyze the use of general ABM codified terms in the scientific literature, the presence of their definitions, and the gap mapping of their use across animal species, categories, years of publication, and geographical areas of the corresponding author's institution. The ultimate aim was to propose a common standard terminology to improve communication among stakeholders. In this study, data models were populated by collecting information coming from scientific papers extracted through a transparent and reproducible protocol using Web of ScienceTM and filtering for the general ABM codified terms (or synonyms/equivalents). A total of 199 papers were retained, and their full texts were assessed. The frequency of general codified ABM terms was analyzed according to the classification factors listed in the objectives. These papers were prevalently European (159 documents), and the most represented species was cattle. Fifty percent of the papers did not provide a definition of the general ABM terms, and 54% cited other sources as reference for their definition. The results of the study showed a very low penetration of the general codified ABM term in the literature on farm animal welfare, with only 1.5% of the papers including the term ABM. This does not mean that specific ABM are not studied, but rather that these specific ABM are not defined as such under a common umbrella, and there is no consensus on the use of terminology, not even among scientists. Thus, we cannot expect the stakeholders to use a common language and a standardized terminology. The recognition and the inclusion of ABM in the lists of commonly accepted abbreviations of scientific journals could be a first step to harmonize the terminology in the scientific literature.

Challenges and Tendencies of Automatic Milking Systems (AMS): A 20-Years Systematic Review of Literature and Patents.

Over the last two decades, the dairy industry has adopted the use of Automatic Milking Systems (AMS). AMS have the potential to increase the effectiveness of the milking process and sustain animal welfare. This study assessed the state of the art of research activities on AMS through a systematic review of scientific and industrial research. The papers and patents of the last 20 years (2000-2019) were analysed to assess the research tendencies. The words appearing in title, abstract and keywords of a total of 802 documents were processed with the text mining tool. Four clusters were identified (Components, Technology, Process and Animal). For each cluster, the words frequency analysis enabled us to identify the research tendencies and gaps. The results showed that focuses of the scientific and industrial research areas complementary, with scientific papers mainly dealing with topics related to animal and process, and patents giving priority to technology and components. Both scientific and industrial research converged on some crucial objectives, such as animal welfare, process sustainability and technological development. Despite the increasing interest in animal welfare, this review highlighted that further progress is needed to meet the consumers' demand. Moreover, milk yield is still regarded as more valuable compared to milk quality. Therefore, additional effort is necessary on the latter. At the process level, some gaps have been found related to cleaning operations, necessary to improve milk quality and animal health. The use of farm data and their incorporation on herd decision support systems (DSS) appeared optimal. The results presented in this review may be used as an overall assessment useful to address future research.

Refining consumer attitudes to milk and dairy product purchase and use to reduce food waste and improve animal welfare on-farm.

This Research Reflection raises awareness of the need to broaden perspectives and levels of multidisciplinary and interdisciplinary approaches when considering on-farm dairy cattle welfare. It starts with a brief overview of current animal welfare issues on dairy farms and how they are perceived by different stakeholders. Some divergences in points of view are discussed in more detail and the first steps in networking are mentioned. Particular emphasis is given to both milk and dairy product waste in industrialized countries and the potential effects of its reduction on changes in the production system. The needs for a quantification of such quota and retailer involvement are also analyzed from the perspective that on-farm animal welfare is directly linked to the amount of milk that might be removed from the food chain by adoption of welfare-friendly management, such as cow-calf systems.

Prevalence of gastrointestinal, liver and claw disorders in veal calves fed large amounts of solid feed through a cross-sectional study.

The impact of the current practice of feeding veal calves with large amounts of solid feed (SF) on the prevalence of specific disorders on rumen, abomasum, liver and claws was investigated through a post-mortem inspection at the abattoir. Forty-one batches ("batch" referred to a group of calves of the same breed, coming from the same farm and belonging to the same slaughter group) of crossbred male calves from dairy breed were randomly inspected at 213.6 days old. On average 16.0 rumens, 15.6 abomasa, 15.1 livers, and 30.5 hind claws were checked per batch. Rumens were evaluated for the presence of hyperkeratosis and plaques; abomasa for the presence of lesions in the pyloric area; livers for the presence of lipidosis, abscess or fibrous adherence; and claws for the presence of sole hemorrhages. More than 60% of rumens per batch had signs of hyperkeratosis and plaques, and 80 to 100% of abomasa per batch showed at least 1 lesion in the pyloric area. On average 24% of livers per batch were diseased and about 65% of claws per batch had sole hemorrhages. Affected abomasa were positively correlated to rumens with plaques. Claws with sole hemorrhages tended to be positively correlated to rumens with hyperkeratosis. Calves of inspected batches were fed 311 ± 31 kg DM/cycle of milk-replacer and 158 ± 44 kg DM/cycle of SF containing more than 85% of corn grain. Based on the recorded prevalence of alterations, this feeding strategy should be reconsidered in order to improve veal calves' welfare.

Effectiveness of Stocking Density Reduction on Mitigating Lameness in a Charolais Finishing Beef Cattle Farm.

This study aimed at assessing whether a reduction in stocking density (SKD) would mitigate lameness and positively affect the performance and health of Charolais bulls in an Italian commercial farm. Bulls were distributed in groups of 10 or 8 animals/pen for high (HD) or low density (LD) corresponding to an individual space of 3.5 or 4.7 m2, respectively. Bulls were fitted with collars that measured rumination time and activity. Three 8-h observational sessions were conducted to record behaviors. Data about health conditions were collected daily. No differences were found in the animals' performance. However, performance results might have been impaired by the culling rate experienced during the trial, which prevented from keeping a consistent SKD. Behaviors did not differ between groups, except for rumination time, which was higher for LD bulls during the third observation (p < 0.05). However, rumination time, recorded by collars, did not vary among treatments. There were no differences in the percentage of sick or lame bulls, but the percentage of animals treated repeatedly due to relapse was higher for the HD group (p < 0.05). It was concluded that a larger space allowance could improve the health of bulls kept on fully slatted floors.

Assessment of Rumen Mucosa, Lung, and Liver Lesions at Slaughter as Benchmarking Tool for the Improvement of Finishing Beef Cattle Health and Welfare.

Abattoir post-mortem inspections offer a useful tool for animal disease surveillance. The present cross-sectional study aimed at assessing the prevalence of rumen mucosa, lung, and liver lesions in 153 randomly selected batches of finishing beef cattle through a post-mortem inspection at the abattoir. At least 15 animals per batch were inspected at slaughter by two veterinarians for a total of 2,161 animals (1,376 bulls; 785 heifers) coming from 80 Italian commercial farms. Rumens were inspected by recording as binary variables (presence/absence) signs of hyperkeratosis, ruminitis, ulcer, and star scars. Similarly, lungs were inspected for signs of pneumonia and livers for signs of lipidosis, abscesses, and/or adherence. Hyperkeratosis of the mucosa and signs of ruminitis were detected in 58 and 30% of the inspected rumens, respectively. Ruminal star scars were more prevalent in bulls than in heifers (18 vs. 11%; P < 0.05). Signs of severe pneumonia were observed in 10% of the lungs; abscess and/or adherence in 4% of the livers. Hyperkeratosis of rumen mucosa was correlated to signs of ruminitis, and signs of ruminitis were correlated to star scars. No correlations were found between hepatic lesions and any other rumen or lung disorders. The wide variability observed among batches for the prevalence of specific lesions suggested the development of a benchmarking system to provide feedback to the farm veterinarians, as these lesions can be reflective of a subclinical disease status not easy to be detected in the live animal. Quartiles of the batch prevalence of rumen, lung, and liver alterations (if ≥1%) were calculated as a benchmarking tool, and third quartile value was proposed as an alarm threshold for each lesion. The use of the benchmarking system could allow to allocate each inspected batch to a specific "health class." Critical batches with a prevalence above the alarm threshold for a given lesion should be reported to veterinarians of the origin farms where actions should be taken in order to identify and lower the risk factors for that specific health issue. Knowledge of post-mortem inspection data along with the implementation of the proposed benchmarking system should help farm veterinarians to improve herd management from a health and welfare perspective.

Risk factors for claw disorders in intensively finished Charolais beef cattle.

This cross-sectional study aimed at performing a risk factor analysis of on-farm housing and management factors associated with infectious and non-infectious claw disorders of intensively finished Charolais young bulls and heifers. Claws' health condition of a total of 1305 animals belonging to 88 batches finished in farms located in the Eastern Po Valley (Italy) was assessed at slaughterhouse. Batch prevalence of feet affected by sole hemorrhage (SH) and white line abscess (WLA) was calculated and foot condition was summarized by infectious lesion (ILS) and non-infectious lesion (NILS) scores according to diseases' etiology. Batch prevalence of feet with SH, WLA, and assigned to the worst score classes were the outcomes in the risk factor analysis. Information about rearing facilities and health management of the tested batches were gathered in each origin farm along with a dietary sample used for chemical and physical analyses. Five categorized factors and 30 continuous covariates were considered as independent predictors and a cluster analysis of the continuous covariates was performed to select the most representative ones. Percentage of feet/batch affected by SH was on average 57.0 ±â€¯32.5 % (SD) and by WLA was 9.94 ±â€¯12.7 %. Percentage of feet/batch with the worst ILS and NILS was 29.6 ±â€¯32.8 % and 12.5 ±â€¯14.1 %, respectively. Charolais bulls showed a higher risk of SH and WLA than heifers and an increasing dietary NDF content acted as preventive factor. Concrete slats increased the risk of SH. Animals slaughtered in winter had the highest risk of WLA and the increasing level of dietary water-soluble carbohydrates (WSC) was a further risk factor. The risk for the worst NILS was lowest in spring and in presence of ventilation systems. It increased for bulls and in those batches fed diets with higher WSC content. Batches for which the farmer reported a higher prevalence of urgently slaughtered animals during finishing showed a higher risk of WLA and infectious claw diseases. The risk for infectious claw diseases was reduced in animals slaughtered in summer, housed on deep litter and fed diet with increased NDF content. It increased in farms where diet formulation was not under the nutritionist control. As general outcomes of the study, benefits for the claw health of finishing Charolaise may come from the use of the deep litter and ventilation systems, the involvement of a nutritionist in diet formulation, and the provision of diets with reduced WSC and increased NDF contents.

Do Dairy Farming Systems Differ in Antimicrobial Use?

The quantitative assessment of antimicrobial use (AMU) in food-producing animals contributes to the provision of essential information for developing relevant and effective policies to reduce use and to control antimicrobial resistance. Information on AMU is available mainly for intensive dairy farming systems and specialized high-yielding breeds. The aim of this study is to investigate AMU in different dairy farming systems by comparing the treatment incidence in mountain farms with specialized high-yield dairy breeds or with dual-purpose breeds raised for milk production to the treatment incidence in lowland farms with specialized high-yield dairy breeds or with dual-purpose breeds raised for milk production. Significant differences were found only between the overall treatment incidence, as well as the treatment incidence of highest-priority critically important antimicrobials for human medicine, in lowland farms with high-yielding breeds and mountain farms with dual-purpose breeds. Mountain farms have a generally lower milk production and smaller herd size than lowland farms, provide cows with access to pasture, and limit concentrates in the diet. These management practices and the use of local/dual-purpose breeds could reduce the risk of production diseases and the consequent need for AMU.

Investigation of a Standardized Qualitative Behaviour Assessment and Exploration of Potential Influencing Factors on the Emotional State of Dairy Calves.

Assessing emotional states of dairy calves is an essential part of welfare assessment, but standardized protocols are absent. The present study aims at assessing the emotional states of dairy calves and establishing a reliable standard procedure with Qualitative Behavioral Assessment (QBA) and 20 defined terms. Video material was used to compare multiple observer results. Further, live observations were performed on 49 dairy herds in Denmark and Italy. Principal Component Analysis (PCA) identified observer agreement and QBA dimensions (PC). For achieving overall welfare judgment, PC1-scores were turned into the Welfare Quality (WQ) criterion 'Positive Emotional State'. Finally, farm factors' influence on the WQ criterion was evaluated by mixed linear models. PCA summarized QBA descriptors as PC1 'Valence' and PC2 'Arousal' (explained variation 40.3% and 13.3%). The highest positive descriptor loadings on PC1 was Happy (0.92) and Nervous (0.72) on PC2. The WQ-criterion score (WQ-C12) was on average 51.1 ± 9.0 points (0: worst to 100: excellent state) and 'Number of calves', 'Farming style', and 'Breed' explained 18% of the variability of it. We conclude that the 20 terms achieved a high portion of explained variation providing a differentiated view on the emotional state of calves. The defined term list proved to need good training for observer agreement.

An overview of claw disorders at slaughter in finishing beef cattle reared in intensive indoor systems through a cross-sectional study.

This cross-sectional study aimed to assess in post-mortem the prevalence of specific claw disorders and their location on the sole in hind feet of finishing beef cattle reared indoors under intensive production systems. Evaluation was made on animals that were introduced in the ordinary slaughterhouse planning, presumably with no signs of impaired locomotion or severe lameness. A total of 4292 hind feet (right and left) belonging to 153 batches were collected (average feet/batch 28.1 ± 5.62 (SD)) in 3 abattoirs in Northern Italy at 3 time points (April-June and September-October 2016; February-March 2017). One veterinarian performed the claw trimming first and then scored the presence of specific claw disorders and their position on the sole considering 7 zones (in the digital and interdigital areas). All claw disorders in a specific zone were recorded as binary (presence/absence). Infectious (ILS), non-infectious (NILS), and global (GLS) scores were calculated considering both the type of claw disorder detected and the number of zones affected. Non-infectious disorders were the most common diagnoses among batches, mainly on the lateral claws and in the heel-sole junction area. In particular, white line abscesses and ulcers (sole and toe ulcers) were also found as two of the most debilitating and painful lesions with a non-negligible frequency. Infectious diseases, when occurring in a batch, spread to almost all feet. As expected, GLS distribution on the total feet inspected showed a non-harmful condition, given that the worst scores from 3 to 13 were assigned to a restricted sample of feet (15%) and were far from the maximum potential value of 50. However, the GLS of all batches monitored revealed 10 critical batches having no healthy feet or more than 50% of feet graded with the worst scores. Since right and left feet of the same animal showed similar clinical diagnoses, a more efficient claw health evaluation system should consider only one foot. Although all cattle inspected were supposed to have no evident locomotory problems before slaughter, the detection of several foot disorders and the considerable distribution in some batches might suggest poor conditions on farms affecting finishing beef cattle health, behaviour, and welfare. It is therefore advisable that possible predisposing factors of specific claw disorders on the farms of origin be investigated more deeply.