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1.
Nat Phys ; 15: 393-402, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30984281

RESUMO

Cell migration over heterogeneous substrates during wound healing or morphogenetic processes leads to shape changes driven by different organizations of the actin cytoskeleton and by functional changes including lamellipodial protrusions and contractile actin cables. Cells distinguish between cell-sized positive and negative curvatures in their physical environment by forming protrusions at positive ones and actin cables at negative ones; however, the cellular mechanisms remain unclear. Here, we report that concave edges promote polarized actin structures with actin flow directed towards the cell edge, in contrast to well-documented retrograde flow at convex edges. Anterograde flow and contractility induce a tension anisotropy gradient. A polarized actin network is formed, accompanied by a local polymerization-depolymerization gradient, together with leading-edge contractile actin cables in the front. These cables extend onto non-adherent regions while still maintaining contact with the substrate through focal adhesions. The contraction and dynamic reorganization of this actin structure allows forward movements enabling cell migration over non-adherent regions on the substrate. These versatile functional structures may help cells sense and navigate their environment by adapting to external geometric and mechanical cues.

2.
Nat Mater ; 16(10): 1029-1037, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28892054

RESUMO

For an organism to develop and maintain homeostasis, cell types with distinct functions must often be separated by physical boundaries. The formation and maintenance of such boundaries are commonly attributed to mechanisms restricted to the cells lining the boundary. Here we show that, besides these local subcellular mechanisms, the formation and maintenance of tissue boundaries involves long-lived, long-ranged mechanical events. Following contact between two epithelial monolayers expressing, respectively, EphB2 and its ligand ephrinB1, both monolayers exhibit oscillatory patterns of traction forces and intercellular stresses that tend to pull cell-matrix adhesions away from the boundary. With time, monolayers jam, accompanied by the emergence of deformation waves that propagate away from the boundary. This phenomenon is not specific to EphB2/ephrinB1 repulsion but is also present during the formation of boundaries with an inert interface and during fusion of homotypic epithelial layers. Our findings thus unveil a global physical mechanism that sustains tissue separation independently of the biochemical and mechanical features of the local tissue boundary.


Assuntos
Relógios Biológicos , Efrina-B1/metabolismo , Células Epiteliais/metabolismo , Matriz Extracelular/metabolismo , Receptor EphB2/metabolismo , Estresse Fisiológico , Animais , Cães , Efrina-B1/genética , Células Epiteliais/citologia , Epitélio/metabolismo , Matriz Extracelular/genética , Células Madin Darby de Rim Canino , Receptor EphB2/genética
3.
Nat Cell Biol ; 19(3): 224-237, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-28218910

RESUMO

Cancer-associated fibroblasts (CAFs) promote tumour invasion and metastasis. We show that CAFs exert a physical force on cancer cells that enables their collective invasion. Force transmission is mediated by a heterophilic adhesion involving N-cadherin at the CAF membrane and E-cadherin at the cancer cell membrane. This adhesion is mechanically active; when subjected to force it triggers ß-catenin recruitment and adhesion reinforcement dependent on α-catenin/vinculin interaction. Impairment of E-cadherin/N-cadherin adhesion abrogates the ability of CAFs to guide collective cell migration and blocks cancer cell invasion. N-cadherin also mediates repolarization of the CAFs away from the cancer cells. In parallel, nectins and afadin are recruited to the cancer cell/CAF interface and CAF repolarization is afadin dependent. Heterotypic junctions between CAFs and cancer cells are observed in patient-derived material. Together, our findings show that a mechanically active heterophilic adhesion between CAFs and cancer cells enables cooperative tumour invasion.


Assuntos
Caderinas/metabolismo , Fibroblastos Associados a Câncer/metabolismo , Fibroblastos Associados a Câncer/patologia , Neoplasias/patologia , Adenocarcinoma/patologia , Adenocarcinoma de Pulmão , Fenômenos Biomecânicos , Fibroblastos Associados a Câncer/ultraestrutura , Adesão Celular , Moléculas de Adesão Celular/metabolismo , Linhagem Celular Tumoral , Ensaios de Migração Celular , Movimento Celular , Polaridade Celular , Técnicas de Cocultura , Feminino , Humanos , Imageamento Tridimensional , Neoplasias Pulmonares/patologia , Mecanotransdução Celular , Proteínas dos Microfilamentos , Nectinas , Invasividade Neoplásica , Neoplasias/metabolismo , Neoplasias de Células Escamosas/patologia , Pinças Ópticas , Esferoides Celulares/patologia , Neoplasias Vulvares/patologia
4.
J Cell Biol ; 212(2): 199-217, 2016 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-26783302

RESUMO

Collective cell migration (CCM) is essential for organism development, wound healing, and metastatic transition, the primary cause of cancer-related death, and it involves cell-cell adhesion molecules of the cadherin family. Increased P-cadherin expression levels are correlated with tumor aggressiveness in carcinoma and aggressive sarcoma; however, how P-cadherin promotes tumor malignancy remains unknown. Here, using integrated cell biology and biophysical approaches, we determined that P-cadherin specifically induces polarization and CCM through an increase in the strength and anisotropy of mechanical forces. We show that this mechanical regulation is mediated by the P-cadherin/ß-PIX/Cdc42 axis; P-cadherin specifically activates Cdc42 through ß-PIX, which is specifically recruited at cell-cell contacts upon CCM. This mechanism of cell polarization and migration is absent in cells expressing E- or R-cadherin. Thus, we identify a specific role of P-cadherin through ß-PIX-mediated Cdc42 activation in the regulation of cell polarity and force anisotropy that drives CCM.


Assuntos
Caderinas/metabolismo , Movimento Celular , Proteína cdc42 de Ligação ao GTP/metabolismo , Animais , Fenômenos Biomecânicos , Polaridade Celular , Células Cultivadas , Camundongos , Mioblastos/citologia , Mioblastos/metabolismo , Fatores de Troca de Nucleotídeo Guanina Rho/metabolismo
5.
Biophys J ; 109(8): 1533-6, 2015 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-26488643

RESUMO

Endothelial cells are constantly exposed to fluid shear stresses that regulate vascular morphogenesis, homeostasis, and disease. The mechanical responses of endothelial cells to relatively high shear flow such as that characteristic of arterial circulation has been extensively studied. Much less is known about the responses of endothelial cells to slow shear flow such as that characteristic of venous circulation, early angiogenesis, atherosclerosis, intracranial aneurysm, or interstitial flow. Here we used a novel, to our knowledge, microfluidic technique to measure traction forces exerted by confluent vascular endothelial cell monolayers under slow shear flow. We found that cells respond to flow with rapid and pronounced increases in traction forces and cell-cell stresses. These responses are reversible in time and do not involve reorientation of the cell body. Traction maps reveal that local cell responses to slow shear flow are highly heterogeneous in magnitude and sign. Our findings unveil a low-flow regime in which endothelial cell mechanics is acutely responsive to shear stress.


Assuntos
Circulação Sanguínea/fisiologia , Células Endoteliais/fisiologia , Estresse Fisiológico/fisiologia , Adaptação Fisiológica/fisiologia , Adesão Celular , Comunicação Celular , Células Cultivadas , Desenho de Equipamento , Humanos , Técnicas Analíticas Microfluídicas/métodos , Microscopia/métodos , Modelos Cardiovasculares , Veias Umbilicais
6.
Nat Commun ; 6: 7683, 2015 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-26158873

RESUMO

Closure of wounds and gaps in tissues is fundamental for the correct development and physiology of multicellular organisms and, when misregulated, may lead to inflammation and tumorigenesis. To re-establish tissue integrity, epithelial cells exhibit coordinated motion into the void by active crawling on the substrate and by constricting a supracellular actomyosin cable. Coexistence of these two mechanisms strongly depends on the environment. However, the nature of their coupling remains elusive because of the complexity of the overall process. Here we demonstrate that epithelial gap geometry in both in vitro and in vivo regulates these collective mechanisms. In addition, the mechanical coupling between actomyosin cable contraction and cell crawling acts as a large-scale regulator to control the dynamics of gap closure. Finally, our computational modelling clarifies the respective roles of the two mechanisms during this process, providing a robust and universal mechanism to explain how epithelial tissues restore their integrity.


Assuntos
Actomiosina/metabolismo , Movimento Celular/fisiologia , Células Epiteliais/fisiologia , Animais , Simulação por Computador , Cães , Drosophila melanogaster , Epitélio , Imunofluorescência , Técnicas In Vitro , Microscopia Intravital , Terapia a Laser , Células Madin Darby de Rim Canino , Microcirurgia , Cicatrização/fisiologia
7.
Nat Commun ; 6: 6111, 2015 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-25608921

RESUMO

The closure of gaps within epithelia is crucial to maintain its integrity during biological processes such as wound healing and gastrulation. Depending on the distribution of extracellular matrix, gap closure occurs through assembly of multicellular actin-based contractile cables or protrusive activity of border cells into the gap. Here we show that the supracellular actomyosin contractility of cells near the gap edge exerts sufficient tension on the surrounding tissue to promote closure of non-adherent gaps. Using traction force microscopy, we observe that cell-generated forces on the substrate at the gap edge first point away from the centre of the gap and then increase in the radial direction pointing into the gap as closure proceeds. Combining with numerical simulations, we show that the increase in force relies less on localized purse-string contractility and more on large-scale remodelling of the suspended tissue around the gap. Our results provide a framework for understanding the assembly and the mechanics of cellular contractility at the tissue level.


Assuntos
Citoesqueleto de Actina/metabolismo , Epitélio/metabolismo , Actinas/química , Actomiosina/metabolismo , Animais , Adesão Celular , Linhagem Celular Tumoral , Proliferação de Células , Simulação por Computador , Cães , Matriz Extracelular/metabolismo , Humanos , Células Madin Darby de Rim Canino , Microscopia de Força Atômica , Microscopia Confocal , Modelos Teóricos
8.
Nat Phys ; 10(9): 683-690, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27340423

RESUMO

A fundamental feature of multicellular organisms is their ability to self-repair wounds through the movement of epithelial cells into the damaged area. This collective cellular movement is commonly attributed to a combination of cell crawling and "purse-string" contraction of a supracellular actomyosin ring. Here we show by direct experimental measurement that these two mechanisms are insufficient to explain force patterns observed during wound closure. At early stages of the process, leading actin protrusions generate traction forces that point away from the wound, showing that wound closure is initially driven by cell crawling. At later stages, we observed unanticipated patterns of traction forces pointing towards the wound. Such patterns have strong force components that are both radial and tangential to the wound. We show that these force components arise from tensions transmitted by a heterogeneous actomyosin ring to the underlying substrate through focal adhesions. The structural and mechanical organization reported here provides cells with a mechanism to close the wound by cooperatively compressing the underlying substrate.

9.
Nat Mater ; 13(1): 87-96, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24292420

RESUMO

The ability of skin to act as a barrier is primarily determined by the efficiency of skin cells to maintain and restore its continuity and integrity. In fact, during wound healing keratinocytes migrate collectively to maintain their cohesion despite heterogeneities in the extracellular matrix. Here, we show that monolayers of human keratinocytes migrating along functionalized micropatterned surfaces comprising alternating strips of extracellular matrix (fibronectin) and non-adherent polymer form suspended multicellular bridges over the non-adherent areas. The bridges are held together by intercellular adhesion and are subjected to considerable tension, as indicated by the presence of prominent actin bundles. We also show that a model based on force propagation through an elastic material reproduces the main features of bridge maintenance and tension distribution. Our findings suggest that multicellular bridges maintain tissue integrity during wound healing when cell-substrate interactions are weak and may prove helpful in the design of artificial scaffolds for skin regeneration.


Assuntos
Movimento Celular , Queratinócitos/citologia , Materiais Biocompatíveis/farmacologia , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Movimento Celular/efeitos dos fármacos , Dimetilpolisiloxanos/farmacologia , Elasticidade , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Fibrinogênio/metabolismo , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Propriedades de Superfície , Alicerces Teciduais
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