1.
Org Biomol Chem
; 4(23): 4387-94, 2006 Dec 07.
Artigo
em Inglês
| MEDLINE
| ID: mdl-17102885
RESUMO
A chemical probe was developed for the detection of the emerging cancer marker galectin-3. The probe contains a benzophenone moiety which covalently attaches itself to the protein upon binding and irradiation. Introduction of a fluorescent label via'click' chemistry allows the labelled proteins to be visualized in a gel. With the probe, selective visualization of galectin-3 in protein mixtures was shown and remarkably even in cell lysates.