[Autofluorescence visualization of the oral malignant lesions]. / Autofluorestsentnaya vizualizatsiya zlokachestvennykh novoobrazovanii slizistoi obolochki rta.

OBJECTIVE: The aim of the present paper was to observe the main features of the autofluorescence emission of the oral epithelial carcinomas. MATERIAL AND METHODS: The study included four patients aged 38-61 years with the oral epithelial carcinomas located at a cheek, a floor of the mouth, a bottom lip. All the diagnoses were later confirmed histologically. The oral cavity was inspected traditionally and then using autofluorescence visualization. For autofluorescence emission excitation we used the home-made LED-based device emitting near 400 nm. RESULTS: The tissue autofluorescence was visualized in the broad and narrow spectral bands. In comparison with the healthy mucosa the pathological lesions showed the large losses of the green autofluorescence emissions ("dark sport effect") which have been visualized at both spectral bands. The visualization in the broad spectral band allowed to reveal that three lesions had the local sites of red fluorescence of the endogenous porphyrins traditionally connected with the local inflammation. CONCLUSION: Autofluorescence stomatoscopy is not only important but also mandatory for oncological screening and should be widely used as the additional method of oral mucosa examination in the common dental practice.

[Role of endogenous porphyrins in laser therapy of experimental skin wounds].

The role of endogenous porphyrins in the effect of laser irradiation on the superoxide dismutase (SOD) activity of wound exudate and rat leukocyte activity has been studied on models of aceptic incised skin wounds. Wounds were irradiated by a He-Ne laser (632.8 nm, 1.5 J/cm2) on the 2nd, 3rd, and 4th days after the beginning of the experiment. Irradiation effects were evaluated by the SOD activity (NBT test) and the activity of leukocytes of wound exudate (as a chemiluminescent response to opsonized zymosan). It was found that in animals subjected to laser irradiation, the SOD activity sharply increased. This effect depended on endogenous porphyrin concentration and was retained throughout the experiment. The SOD activity in unirradiated animals decreased from the 2nd to the 5th day of experiment. The evaluation of the activity of wound exudate leukocytes did not reveal any distrinct dependence of the effect on the concentration of endogenous porphyrins.

[Fluorescence detection of chlorine E6 based photosensitizer in urinary bladder tumors].

The authors present pilot experience with accumulation and distribution of chlorine E6 based photosensitizer in unaffected mucosa and tumor of human urinary bladder (UB). Local fluorescence spectroscopy in vivo has shown that 2-3 hours after intravenous injection of the photosensitizer in a dose 1 mg/kg its exogenic fluorescence is detected in the tumor but not in unaffected UB mucosa. This indicates selective accumulation of the sensitizer in UB tumors. Thus, chlorine E6 based photosensitizer can be used for fluorescence-based diagnosis of UB cancer and treatment of such patients with photodynamic therapy. Application of local fluorescence spectroscopy allows monitoring of photobleaching in the course of treatment session.

[Fluorescence detection of photosensitizer photoditazin in the tissue of benign prostatic hyperplasia].

The authors present pilot experience in investigation of accumulation, distribution and elimination of photosensitizer photoditazin from hyperplastic tissue of human prostate. Fluorescent spectroscopy showed that 2-24 hours after intravenous injection of photoditazin in a dose 1 mg/kg, it is detected in hyperplastic tissue of human prostate with maximal accumulation 3 hours after introduction. Exogenic fluorescence of photoditazin lowered 12 and 24 hours after injection this indicating its elimination. The results favor use of photoditazin for photodynamic treatment of patients with benign prostatic hyperplasia.